Experiment: Tween 20 (C), 20C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS00960 and A4249_RS00965 are separated by 100 nucleotides A4249_RS00965 and A4249_RS00970 overlap by 1 nucleotides A4249_RS00970 and A4249_RS00975 are separated by 129 nucleotides A4249_RS00975 and A4249_RS00980 are separated by 209 nucleotides
A4249_RS00960: A4249_RS00960 - fasciclin domain-containing protein, at 178,954 to 179,850
_RS00960
A4249_RS00965: A4249_RS00965 - glycine--tRNA ligase subunit alpha, at 179,951 to 180,844
_RS00965
A4249_RS00970: A4249_RS00970 - glycine--tRNA ligase subunit beta, at 180,844 to 183,009
_RS00970
A4249_RS00975: A4249_RS00975 - DUF559 domain-containing protein, at 183,139 to 183,492
_RS00975
A4249_RS00980: A4249_RS00980 - pyruvate, phosphate dikinase, at 183,702 to 186,383
_RS00980
Position (kb) 180
181
182
183
184 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3 at 179.908 kb on - strand at 179.908 kb on - strand at 180.815 kb on + strand at 183.140 kb on + strand at 183.140 kb on + strand at 183.140 kb on + strand at 183.140 kb on + strand at 183.140 kb on + strand at 183.141 kb on - strand at 183.141 kb on - strand at 183.141 kb on - strand at 183.141 kb on - strand at 183.141 kb on - strand at 183.141 kb on - strand at 183.338 kb on + strand, within A4249_RS00975 at 183.338 kb on + strand, within A4249_RS00975 at 183.338 kb on + strand, within A4249_RS00975 at 183.339 kb on - strand, within A4249_RS00975 at 183.339 kb on - strand, within A4249_RS00975 at 183.339 kb on - strand, within A4249_RS00975 at 183.339 kb on - strand, within A4249_RS00975 at 183.339 kb on - strand, within A4249_RS00975 at 183.353 kb on + strand, within A4249_RS00975 at 183.353 kb on + strand, within A4249_RS00975 at 183.353 kb on + strand, within A4249_RS00975 at 183.353 kb on + strand, within A4249_RS00975 at 183.353 kb on + strand, within A4249_RS00975 at 183.353 kb on + strand, within A4249_RS00975 at 183.353 kb on + strand, within A4249_RS00975 at 183.353 kb on + strand, within A4249_RS00975 at 183.354 kb on - strand, within A4249_RS00975 at 183.354 kb on - strand, within A4249_RS00975 at 183.354 kb on - strand, within A4249_RS00975 at 183.354 kb on - strand, within A4249_RS00975 at 183.354 kb on - strand, within A4249_RS00975 at 183.354 kb on - strand, within A4249_RS00975 at 183.354 kb on - strand, within A4249_RS00975 at 183.354 kb on - strand, within A4249_RS00975 at 183.354 kb on - strand, within A4249_RS00975 at 183.354 kb on - strand, within A4249_RS00975 at 183.574 kb on + strand at 183.574 kb on + strand at 183.575 kb on - strand at 183.575 kb on - strand at 183.575 kb on - strand at 183.575 kb on - strand at 183.575 kb on - strand at 183.604 kb on + strand at 183.604 kb on + strand at 183.605 kb on - strand at 183.605 kb on - strand at 183.605 kb on - strand at 183.605 kb on - strand at 183.638 kb on + strand at 183.638 kb on + strand at 183.638 kb on + strand at 183.638 kb on + strand at 183.639 kb on - strand at 183.639 kb on - strand at 183.720 kb on + strand at 183.720 kb on + strand at 183.720 kb on + strand at 183.721 kb on - strand at 183.721 kb on - strand at 183.721 kb on - strand at 183.721 kb on - strand at 183.721 kb on - strand at 183.721 kb on - strand at 183.721 kb on - strand at 183.721 kb on - strand at 183.864 kb on + strand at 183.864 kb on + strand at 183.865 kb on - strand at 183.865 kb on - strand at 183.865 kb on - strand at 183.867 kb on + strand at 183.867 kb on + strand at 183.867 kb on + strand at 183.867 kb on + strand at 183.867 kb on + strand at 183.867 kb on + strand at 183.867 kb on + strand at 183.867 kb on + strand at 183.867 kb on + strand at 183.867 kb on + strand at 183.867 kb on + strand at 183.867 kb on + strand at 183.867 kb on + strand at 183.867 kb on + strand at 183.867 kb on + strand at 183.868 kb on - strand at 183.868 kb on - strand at 183.868 kb on - strand at 183.868 kb on - strand at 183.868 kb on - strand at 183.868 kb on - strand at 183.868 kb on - strand at 183.868 kb on - strand at 183.868 kb on - strand at 183.872 kb on + strand at 183.873 kb on - strand at 183.885 kb on + strand at 183.885 kb on + strand at 183.886 kb on - strand at 183.886 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C), 20C remove 179,908 - +2.6 179,908 - -1.0 180,815 + +0.0 183,140 + +0.2 183,140 + -1.7 183,140 + -1.2 183,140 + +0.0 183,140 + +0.4 183,141 - +0.8 183,141 - -0.3 183,141 - +1.2 183,141 - -2.0 183,141 - +0.6 183,141 - -0.1 183,338 + A4249_RS00975 0.56 -0.5 183,338 + A4249_RS00975 0.56 -1.0 183,338 + A4249_RS00975 0.56 -2.0 183,339 - A4249_RS00975 0.56 -0.1 183,339 - A4249_RS00975 0.56 -1.6 183,339 - A4249_RS00975 0.56 -1.5 183,339 - A4249_RS00975 0.56 -1.4 183,339 - A4249_RS00975 0.56 +1.2 183,353 + A4249_RS00975 0.60 -2.3 183,353 + A4249_RS00975 0.60 -2.2 183,353 + A4249_RS00975 0.60 +0.0 183,353 + A4249_RS00975 0.60 +0.4 183,353 + A4249_RS00975 0.60 -0.6 183,353 + A4249_RS00975 0.60 -2.3 183,353 + A4249_RS00975 0.60 -0.0 183,353 + A4249_RS00975 0.60 -0.4 183,354 - A4249_RS00975 0.61 +0.9 183,354 - A4249_RS00975 0.61 +0.0 183,354 - A4249_RS00975 0.61 -0.0 183,354 - A4249_RS00975 0.61 -1.0 183,354 - A4249_RS00975 0.61 -0.6 183,354 - A4249_RS00975 0.61 -0.3 183,354 - A4249_RS00975 0.61 -1.3 183,354 - A4249_RS00975 0.61 +0.0 183,354 - A4249_RS00975 0.61 -1.6 183,354 - A4249_RS00975 0.61 -2.0 183,574 + -3.3 183,574 + +0.7 183,575 - -0.5 183,575 - -0.1 183,575 - -0.6 183,575 - +0.1 183,575 - +0.3 183,604 + +1.2 183,604 + -0.4 183,605 - +0.0 183,605 - -1.4 183,605 - +2.8 183,605 - +1.4 183,638 + -1.3 183,638 + -2.8 183,638 + -3.4 183,638 + -2.9 183,639 - -4.7 183,639 - -1.0 183,720 + -3.4 183,720 + -5.9 183,720 + -4.1 183,721 - -4.5 183,721 - -3.4 183,721 - -3.9 183,721 - -4.4 183,721 - -2.3 183,721 - -3.4 183,721 - -4.9 183,721 - -4.2 183,864 + -3.8 183,864 + -4.0 183,865 - -3.1 183,865 - -5.3 183,865 - -5.4 183,867 + -2.6 183,867 + -4.3 183,867 + -3.7 183,867 + -4.7 183,867 + -4.3 183,867 + -4.5 183,867 + -2.6 183,867 + -4.7 183,867 + -3.0 183,867 + -4.4 183,867 + -4.9 183,867 + -4.9 183,867 + -3.9 183,867 + -3.9 183,867 + -4.4 183,868 - +0.0 183,868 - -3.3 183,868 - -2.6 183,868 - -1.6 183,868 - -3.3 183,868 - -4.4 183,868 - -4.1 183,868 - -5.3 183,868 - -4.7 183,872 + -5.6 183,873 - -5.3 183,885 + -3.4 183,885 + -3.6 183,886 - -4.6 183,886 - -4.4
Or see this region's nucleotide sequence