Strain Fitness in Pseudomonas orientalis W4I3 around QF045_RS04335

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntQF045_RS04330 and QF045_RS04335 are separated by 121 nucleotidesQF045_RS04335 and QF045_RS04340 are separated by 179 nucleotides QF045_RS04330: QF045_RS04330 - phosphatase PAP2 family protein, at 972,422 to 973,225 _RS04330 QF045_RS04335: QF045_RS04335 - Hsp33 family molecular chaperone HslO, at 973,347 to 974,249 _RS04335 QF045_RS04340: QF045_RS04340 - phosphoenolpyruvate carboxykinase, at 974,429 to 975,970 _RS04340 Position (kb) 973 974 975Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 972.397 kb on - strandat 972.397 kb on - strandat 972.397 kb on - strandat 972.397 kb on - strandat 972.421 kb on + strandat 972.421 kb on + strandat 972.429 kb on - strandat 972.476 kb on - strandat 972.512 kb on + strand, within QF045_RS04330at 972.512 kb on - strand, within QF045_RS04330at 972.539 kb on + strand, within QF045_RS04330at 972.539 kb on + strand, within QF045_RS04330at 972.546 kb on + strand, within QF045_RS04330at 972.554 kb on - strand, within QF045_RS04330at 972.554 kb on - strand, within QF045_RS04330at 972.557 kb on + strand, within QF045_RS04330at 972.598 kb on + strand, within QF045_RS04330at 972.803 kb on + strand, within QF045_RS04330at 972.803 kb on + strand, within QF045_RS04330at 972.803 kb on + strand, within QF045_RS04330at 972.811 kb on - strand, within QF045_RS04330at 972.811 kb on - strand, within QF045_RS04330at 972.839 kb on + strand, within QF045_RS04330at 972.849 kb on + strand, within QF045_RS04330at 972.849 kb on + strand, within QF045_RS04330at 972.931 kb on - strand, within QF045_RS04330at 972.948 kb on + strand, within QF045_RS04330at 972.950 kb on + strand, within QF045_RS04330at 972.956 kb on - strand, within QF045_RS04330at 972.956 kb on - strand, within QF045_RS04330at 972.987 kb on - strand, within QF045_RS04330at 973.029 kb on - strand, within QF045_RS04330at 973.039 kb on + strand, within QF045_RS04330at 973.114 kb on + strand, within QF045_RS04330at 973.135 kb on + strand, within QF045_RS04330at 973.174 kb on + strandat 973.244 kb on + strandat 973.250 kb on - strandat 973.352 kb on - strandat 973.496 kb on + strand, within QF045_RS04335at 973.496 kb on + strand, within QF045_RS04335at 973.504 kb on - strand, within QF045_RS04335at 973.515 kb on + strand, within QF045_RS04335at 973.554 kb on + strand, within QF045_RS04335at 973.608 kb on - strand, within QF045_RS04335at 973.608 kb on - strand, within QF045_RS04335at 973.748 kb on + strand, within QF045_RS04335at 973.755 kb on + strand, within QF045_RS04335at 973.763 kb on - strand, within QF045_RS04335at 973.883 kb on + strand, within QF045_RS04335at 973.889 kb on + strand, within QF045_RS04335at 973.891 kb on - strand, within QF045_RS04335at 973.934 kb on + strand, within QF045_RS04335at 973.965 kb on + strand, within QF045_RS04335at 973.991 kb on - strand, within QF045_RS04335at 973.991 kb on - strand, within QF045_RS04335at 974.079 kb on + strand, within QF045_RS04335at 974.082 kb on - strand, within QF045_RS04335at 974.087 kb on - strand, within QF045_RS04335at 974.087 kb on - strand, within QF045_RS04335at 974.089 kb on + strand, within QF045_RS04335at 974.092 kb on - strand, within QF045_RS04335at 974.097 kb on - strand, within QF045_RS04335at 974.097 kb on - strand, within QF045_RS04335at 974.097 kb on - strand, within QF045_RS04335at 974.185 kb on - strandat 974.204 kb on - strandat 974.204 kb on - strandat 974.219 kb on + strandat 974.270 kb on - strandat 974.297 kb on + strandat 974.406 kb on + strandat 974.406 kb on + strandat 974.406 kb on + strandat 974.414 kb on - strandat 974.414 kb on - strandat 974.487 kb on - strandat 974.502 kb on + strandat 974.599 kb on - strand, within QF045_RS04340at 974.653 kb on + strand, within QF045_RS04340at 974.689 kb on - strand, within QF045_RS04340at 974.835 kb on + strand, within QF045_RS04340at 974.860 kb on - strand, within QF045_RS04340at 975.109 kb on - strand, within QF045_RS04340at 975.218 kb on - strand, within QF045_RS04340

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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972,397 - -0.2
972,397 - +0.5
972,397 - +0.9
972,397 - +0.2
972,421 + +0.5
972,421 + -2.7
972,429 - -0.5
972,476 - +0.1
972,512 + QF045_RS04330 0.11 -1.5
972,512 - QF045_RS04330 0.11 -1.1
972,539 + QF045_RS04330 0.15 -2.0
972,539 + QF045_RS04330 0.15 -0.6
972,546 + QF045_RS04330 0.15 +0.4
972,554 - QF045_RS04330 0.16 +0.4
972,554 - QF045_RS04330 0.16 -0.2
972,557 + QF045_RS04330 0.17 -0.2
972,598 + QF045_RS04330 0.22 +0.1
972,803 + QF045_RS04330 0.47 -0.1
972,803 + QF045_RS04330 0.47 -1.4
972,803 + QF045_RS04330 0.47 -2.1
972,811 - QF045_RS04330 0.48 -2.7
972,811 - QF045_RS04330 0.48 -0.8
972,839 + QF045_RS04330 0.52 -1.3
972,849 + QF045_RS04330 0.53 -2.8
972,849 + QF045_RS04330 0.53 -2.7
972,931 - QF045_RS04330 0.63 -2.2
972,948 + QF045_RS04330 0.65 +0.5
972,950 + QF045_RS04330 0.66 -0.3
972,956 - QF045_RS04330 0.66 +1.0
972,956 - QF045_RS04330 0.66 +0.5
972,987 - QF045_RS04330 0.70 -1.5
973,029 - QF045_RS04330 0.75 -1.3
973,039 + QF045_RS04330 0.77 +0.9
973,114 + QF045_RS04330 0.86 -0.9
973,135 + QF045_RS04330 0.89 -2.5
973,174 + +0.2
973,244 + -2.5
973,250 - +0.5
973,352 - -2.6
973,496 + QF045_RS04335 0.17 -0.2
973,496 + QF045_RS04335 0.17 -2.7
973,504 - QF045_RS04335 0.17 -1.1
973,515 + QF045_RS04335 0.19 +0.8
973,554 + QF045_RS04335 0.23 -0.3
973,608 - QF045_RS04335 0.29 -2.1
973,608 - QF045_RS04335 0.29 -2.3
973,748 + QF045_RS04335 0.44 +0.5
973,755 + QF045_RS04335 0.45 -1.8
973,763 - QF045_RS04335 0.46 -3.0
973,883 + QF045_RS04335 0.59 -1.8
973,889 + QF045_RS04335 0.60 -2.1
973,891 - QF045_RS04335 0.60 -0.7
973,934 + QF045_RS04335 0.65 -1.8
973,965 + QF045_RS04335 0.68 -1.8
973,991 - QF045_RS04335 0.71 +0.0
973,991 - QF045_RS04335 0.71 +0.2
974,079 + QF045_RS04335 0.81 -0.5
974,082 - QF045_RS04335 0.81 +0.8
974,087 - QF045_RS04335 0.82 -0.8
974,087 - QF045_RS04335 0.82 -1.1
974,089 + QF045_RS04335 0.82 -1.8
974,092 - QF045_RS04335 0.83 +0.5
974,097 - QF045_RS04335 0.83 -0.1
974,097 - QF045_RS04335 0.83 -1.8
974,097 - QF045_RS04335 0.83 -1.3
974,185 - +0.5
974,204 - +0.5
974,204 - +0.5
974,219 + +2.1
974,270 - +0.7
974,297 + +0.5
974,406 + +0.7
974,406 + -1.5
974,406 + +0.4
974,414 - +0.7
974,414 - +0.0
974,487 - -0.1
974,502 + -0.4
974,599 - QF045_RS04340 0.11 -2.3
974,653 + QF045_RS04340 0.15 +0.1
974,689 - QF045_RS04340 0.17 -1.4
974,835 + QF045_RS04340 0.26 -2.5
974,860 - QF045_RS04340 0.28 -0.5
975,109 - QF045_RS04340 0.44 -1.1
975,218 - QF045_RS04340 0.51 +0.3

Or see this region's nucleotide sequence