Experiment: PCN 300 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QF045_RS04330 and QF045_RS04335 are separated by 121 nucleotides QF045_RS04335 and QF045_RS04340 are separated by 179 nucleotides
QF045_RS04330: QF045_RS04330 - phosphatase PAP2 family protein, at 972,422 to 973,225
_RS04330
QF045_RS04335: QF045_RS04335 - Hsp33 family molecular chaperone HslO, at 973,347 to 974,249
_RS04335
QF045_RS04340: QF045_RS04340 - phosphoenolpyruvate carboxykinase, at 974,429 to 975,970
_RS04340
Position (kb) 973
974
975 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 972.397 kb on - strand at 972.397 kb on - strand at 972.397 kb on - strand at 972.397 kb on - strand at 972.421 kb on + strand at 972.421 kb on + strand at 972.429 kb on - strand at 972.476 kb on - strand at 972.512 kb on + strand, within QF045_RS04330 at 972.512 kb on - strand, within QF045_RS04330 at 972.539 kb on + strand, within QF045_RS04330 at 972.539 kb on + strand, within QF045_RS04330 at 972.546 kb on + strand, within QF045_RS04330 at 972.554 kb on - strand, within QF045_RS04330 at 972.554 kb on - strand, within QF045_RS04330 at 972.557 kb on + strand, within QF045_RS04330 at 972.598 kb on + strand, within QF045_RS04330 at 972.803 kb on + strand, within QF045_RS04330 at 972.803 kb on + strand, within QF045_RS04330 at 972.803 kb on + strand, within QF045_RS04330 at 972.811 kb on - strand, within QF045_RS04330 at 972.811 kb on - strand, within QF045_RS04330 at 972.839 kb on + strand, within QF045_RS04330 at 972.849 kb on + strand, within QF045_RS04330 at 972.849 kb on + strand, within QF045_RS04330 at 972.931 kb on - strand, within QF045_RS04330 at 972.948 kb on + strand, within QF045_RS04330 at 972.950 kb on + strand, within QF045_RS04330 at 972.956 kb on - strand, within QF045_RS04330 at 972.956 kb on - strand, within QF045_RS04330 at 972.987 kb on - strand, within QF045_RS04330 at 973.029 kb on - strand, within QF045_RS04330 at 973.039 kb on + strand, within QF045_RS04330 at 973.114 kb on + strand, within QF045_RS04330 at 973.135 kb on + strand, within QF045_RS04330 at 973.174 kb on + strand at 973.244 kb on + strand at 973.250 kb on - strand at 973.352 kb on - strand at 973.496 kb on + strand, within QF045_RS04335 at 973.496 kb on + strand, within QF045_RS04335 at 973.504 kb on - strand, within QF045_RS04335 at 973.515 kb on + strand, within QF045_RS04335 at 973.554 kb on + strand, within QF045_RS04335 at 973.608 kb on - strand, within QF045_RS04335 at 973.608 kb on - strand, within QF045_RS04335 at 973.748 kb on + strand, within QF045_RS04335 at 973.755 kb on + strand, within QF045_RS04335 at 973.763 kb on - strand, within QF045_RS04335 at 973.883 kb on + strand, within QF045_RS04335 at 973.889 kb on + strand, within QF045_RS04335 at 973.891 kb on - strand, within QF045_RS04335 at 973.934 kb on + strand, within QF045_RS04335 at 973.965 kb on + strand, within QF045_RS04335 at 973.991 kb on - strand, within QF045_RS04335 at 973.991 kb on - strand, within QF045_RS04335 at 974.079 kb on + strand, within QF045_RS04335 at 974.082 kb on - strand, within QF045_RS04335 at 974.087 kb on - strand, within QF045_RS04335 at 974.087 kb on - strand, within QF045_RS04335 at 974.089 kb on + strand, within QF045_RS04335 at 974.092 kb on - strand, within QF045_RS04335 at 974.097 kb on - strand, within QF045_RS04335 at 974.097 kb on - strand, within QF045_RS04335 at 974.097 kb on - strand, within QF045_RS04335 at 974.185 kb on - strand at 974.204 kb on - strand at 974.204 kb on - strand at 974.219 kb on + strand at 974.270 kb on - strand at 974.297 kb on + strand at 974.406 kb on + strand at 974.406 kb on + strand at 974.406 kb on + strand at 974.414 kb on - strand at 974.414 kb on - strand at 974.487 kb on - strand at 974.502 kb on + strand at 974.599 kb on - strand, within QF045_RS04340 at 974.653 kb on + strand, within QF045_RS04340 at 974.689 kb on - strand, within QF045_RS04340 at 974.835 kb on + strand, within QF045_RS04340 at 974.860 kb on - strand, within QF045_RS04340 at 975.109 kb on - strand, within QF045_RS04340 at 975.218 kb on - strand, within QF045_RS04340
Per-strain Table
Position Strand Gene LocusTag Fraction PCN 300 uM remove 972,397 - -1.4 972,397 - -0.2 972,397 - -0.1 972,397 - +0.4 972,421 + +1.2 972,421 + -1.5 972,429 - +0.8 972,476 - -0.7 972,512 + QF045_RS04330 0.11 +0.0 972,512 - QF045_RS04330 0.11 +0.1 972,539 + QF045_RS04330 0.15 -1.2 972,539 + QF045_RS04330 0.15 -0.9 972,546 + QF045_RS04330 0.15 -0.5 972,554 - QF045_RS04330 0.16 -0.3 972,554 - QF045_RS04330 0.16 -1.2 972,557 + QF045_RS04330 0.17 +0.1 972,598 + QF045_RS04330 0.22 -0.5 972,803 + QF045_RS04330 0.47 -1.9 972,803 + QF045_RS04330 0.47 -0.5 972,803 + QF045_RS04330 0.47 -0.4 972,811 - QF045_RS04330 0.48 +0.6 972,811 - QF045_RS04330 0.48 -0.1 972,839 + QF045_RS04330 0.52 +1.2 972,849 + QF045_RS04330 0.53 -1.7 972,849 + QF045_RS04330 0.53 +0.5 972,931 - QF045_RS04330 0.63 +0.1 972,948 + QF045_RS04330 0.65 +4.1 972,950 + QF045_RS04330 0.66 -0.2 972,956 - QF045_RS04330 0.66 +0.3 972,956 - QF045_RS04330 0.66 +2.0 972,987 - QF045_RS04330 0.70 -0.4 973,029 - QF045_RS04330 0.75 -0.2 973,039 + QF045_RS04330 0.77 -0.3 973,114 + QF045_RS04330 0.86 -0.5 973,135 + QF045_RS04330 0.89 -2.4 973,174 + -0.3 973,244 + -2.4 973,250 - -0.1 973,352 - -0.9 973,496 + QF045_RS04335 0.17 -0.4 973,496 + QF045_RS04335 0.17 -0.2 973,504 - QF045_RS04335 0.17 -0.9 973,515 + QF045_RS04335 0.19 -1.3 973,554 + QF045_RS04335 0.23 -0.6 973,608 - QF045_RS04335 0.29 -0.9 973,608 - QF045_RS04335 0.29 -0.6 973,748 + QF045_RS04335 0.44 +1.7 973,755 + QF045_RS04335 0.45 -1.7 973,763 - QF045_RS04335 0.46 -0.5 973,883 + QF045_RS04335 0.59 -1.9 973,889 + QF045_RS04335 0.60 -0.3 973,891 - QF045_RS04335 0.60 +0.4 973,934 + QF045_RS04335 0.65 -0.7 973,965 + QF045_RS04335 0.68 -1.7 973,991 - QF045_RS04335 0.71 -0.2 973,991 - QF045_RS04335 0.71 -0.1 974,079 + QF045_RS04335 0.81 -0.3 974,082 - QF045_RS04335 0.81 -0.5 974,087 - QF045_RS04335 0.82 -0.3 974,087 - QF045_RS04335 0.82 +0.7 974,089 + QF045_RS04335 0.82 +0.7 974,092 - QF045_RS04335 0.83 -0.3 974,097 - QF045_RS04335 0.83 -0.6 974,097 - QF045_RS04335 0.83 -0.7 974,097 - QF045_RS04335 0.83 +1.5 974,185 - +0.7 974,204 - -0.1 974,204 - -0.4 974,219 + +0.7 974,270 - +0.0 974,297 + -0.2 974,406 + +0.2 974,406 + -1.3 974,406 + -0.5 974,414 - +1.0 974,414 - -0.4 974,487 - -0.3 974,502 + -0.4 974,599 - QF045_RS04340 0.11 -1.2 974,653 + QF045_RS04340 0.15 -0.7 974,689 - QF045_RS04340 0.17 +0.7 974,835 + QF045_RS04340 0.26 +0.2 974,860 - QF045_RS04340 0.28 +2.2 975,109 - QF045_RS04340 0.44 -1.5 975,218 - QF045_RS04340 0.51 +0.2
Or see this region's nucleotide sequence