Experiment: D-Glucose and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1766 and GFF1767 are separated by 4 nucleotides GFF1767 and GFF1768 are separated by 68 nucleotides GFF1768 and GFF1769 overlap by 4 nucleotides
GFF1766 - GTPase Obg, at 403,605 to 404,657
GFF1766
GFF1767 - Glutamate 5-kinase, at 404,662 to 405,783
GFF1767
GFF1768 - N5-carboxyaminoimidazole ribonucleotide mutase, at 405,852 to 406,346
GFF1768
GFF1769 - N5-carboxyaminoimidazole ribonucleotide synthase, at 406,343 to 407,491
GFF1769
Position (kb) 404
405
406 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 404.596 kb on + strand at 405.507 kb on + strand, within GFF1767 at 405.507 kb on + strand, within GFF1767 at 405.683 kb on + strand at 405.683 kb on + strand at 405.683 kb on + strand at 405.716 kb on + strand at 405.716 kb on + strand at 405.755 kb on + strand at 405.755 kb on + strand at 405.755 kb on + strand at 405.755 kb on + strand at 405.779 kb on + strand at 405.779 kb on + strand at 405.779 kb on + strand at 405.779 kb on + strand at 405.779 kb on + strand at 405.779 kb on + strand at 405.779 kb on + strand at 405.779 kb on + strand at 405.781 kb on + strand at 405.781 kb on + strand at 405.781 kb on + strand at 405.781 kb on + strand at 405.791 kb on + strand at 405.791 kb on + strand at 405.791 kb on + strand at 405.791 kb on + strand at 405.791 kb on + strand at 405.791 kb on + strand at 405.791 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose and casaminos (C) remove 404,596 + -1.8 405,507 + GFF1767 0.75 -2.0 405,507 + GFF1767 0.75 -0.2 405,683 + -1.0 405,683 + -1.8 405,683 + -3.1 405,716 + -1.0 405,716 + -0.0 405,755 + -1.3 405,755 + -1.6 405,755 + -1.0 405,755 + -1.7 405,779 + +0.2 405,779 + +0.2 405,779 + -0.3 405,779 + -0.0 405,779 + -0.7 405,779 + -1.0 405,779 + -1.6 405,779 + +0.6 405,781 + -0.3 405,781 + -0.3 405,781 + -0.9 405,781 + -0.4 405,791 + +0.8 405,791 + -1.0 405,791 + +1.3 405,791 + -1.3 405,791 + -0.3 405,791 + -1.6 405,791 + -1.3
Or see this region's nucleotide sequence