Experiment: FE-KB2-T1 (Control KB Rep 2 Time 1)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OLJFJH_05560 and OLJFJH_05565 are separated by 19 nucleotides OLJFJH_05565 and OLJFJH_05570 are separated by 56 nucleotides OLJFJH_05570 and OLJFJH_05575 are separated by 259 nucleotides
OLJFJH_05560: OLJFJH_05560 - bifunctional GTP diphosphokinase/guanosine-3' 5'-bis pyrophosphate 3'-pyrophosphohydrolase, at 1,133,229 to 1,135,352
_05560
OLJFJH_05565: OLJFJH_05565 - DNA-directed RNA polymerase subunit omega, at 1,135,372 to 1,135,647
_05565
OLJFJH_05570: OLJFJH_05570 - guanylate kinase, at 1,135,704 to 1,136,327
_05570
OLJFJH_05575: OLJFJH_05575 - NAD-dependent DNA ligase LigB, at 1,136,587 to 1,138,251
_05575
Position (kb)
1135
1136 Strain fitness (log2 ratio)
-2
-1
0
1 at 1135.349 kb on - strand at 1135.619 kb on - strand, within OLJFJH_05565 at 1135.648 kb on - strand at 1135.648 kb on - strand at 1135.657 kb on - strand at 1136.375 kb on - strand at 1136.375 kb on - strand at 1136.377 kb on - strand at 1136.377 kb on - strand at 1136.423 kb on + strand at 1136.449 kb on + strand at 1136.536 kb on + strand at 1136.537 kb on - strand at 1136.537 kb on - strand at 1136.537 kb on - strand at 1136.567 kb on + strand at 1136.568 kb on - strand at 1136.568 kb on - strand at 1136.594 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction FE-KB2-T1 (Control KB Rep 2 Time 1) remove 1,135,349 - -0.3 1,135,619 - OLJFJH_05565 0.89 -1.1 1,135,648 - -0.3 1,135,648 - -1.8 1,135,657 - -1.1 1,136,375 - +0.1 1,136,375 - -0.3 1,136,377 - -0.2 1,136,377 - +0.5 1,136,423 + +0.0 1,136,449 + -0.1 1,136,536 + +0.4 1,136,537 - +0.3 1,136,537 - -0.1 1,136,537 - +0.0 1,136,567 + +0.4 1,136,568 - +0.0 1,136,568 - -0.1 1,136,594 - -0.0
Or see this region's nucleotide sequence