Experiment: NL-CCM_agar
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX19_01045 and MPMX19_01046 are separated by 354 nucleotides MPMX19_01046 and MPMX19_01047 are separated by 716 nucleotides
MPMX19_01045: MPMX19_01045 - hypothetical protein, at 1,084,986 to 1,086,332
_01045
MPMX19_01046: MPMX19_01046 - RNA polymerase sigma factor RpoH, at 1,086,687 to 1,087,577
_01046
MPMX19_01047: MPMX19_01047 - Transcriptional regulatory protein ros, at 1,088,294 to 1,088,704
_01047
Position (kb)
1086
1087
1088 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1085.858 kb on + strand, within MPMX19_01045 at 1085.859 kb on - strand, within MPMX19_01045 at 1085.859 kb on - strand, within MPMX19_01045 at 1085.859 kb on - strand, within MPMX19_01045 at 1085.859 kb on - strand, within MPMX19_01045 at 1085.860 kb on + strand, within MPMX19_01045 at 1085.860 kb on + strand, within MPMX19_01045 at 1085.860 kb on + strand, within MPMX19_01045 at 1085.860 kb on + strand, within MPMX19_01045 at 1085.860 kb on + strand, within MPMX19_01045 at 1085.860 kb on + strand, within MPMX19_01045 at 1085.860 kb on + strand, within MPMX19_01045 at 1085.860 kb on + strand, within MPMX19_01045 at 1085.860 kb on + strand, within MPMX19_01045 at 1085.860 kb on + strand, within MPMX19_01045 at 1085.860 kb on + strand, within MPMX19_01045 at 1085.860 kb on + strand, within MPMX19_01045 at 1085.860 kb on + strand, within MPMX19_01045 at 1085.860 kb on + strand, within MPMX19_01045 at 1085.860 kb on + strand, within MPMX19_01045 at 1085.860 kb on + strand, within MPMX19_01045 at 1085.861 kb on - strand, within MPMX19_01045 at 1085.861 kb on - strand, within MPMX19_01045 at 1085.861 kb on - strand, within MPMX19_01045 at 1085.861 kb on - strand, within MPMX19_01045 at 1085.861 kb on - strand, within MPMX19_01045 at 1085.861 kb on - strand, within MPMX19_01045 at 1085.861 kb on - strand, within MPMX19_01045 at 1085.861 kb on - strand, within MPMX19_01045 at 1085.861 kb on - strand, within MPMX19_01045 at 1085.861 kb on - strand, within MPMX19_01045 at 1085.861 kb on - strand, within MPMX19_01045 at 1085.861 kb on - strand, within MPMX19_01045 at 1085.992 kb on + strand, within MPMX19_01045 at 1085.992 kb on + strand, within MPMX19_01045 at 1085.992 kb on + strand, within MPMX19_01045 at 1085.992 kb on + strand, within MPMX19_01045 at 1085.993 kb on - strand, within MPMX19_01045 at 1085.993 kb on - strand, within MPMX19_01045 at 1085.993 kb on - strand, within MPMX19_01045 at 1085.993 kb on - strand, within MPMX19_01045 at 1086.004 kb on + strand, within MPMX19_01045 at 1086.004 kb on + strand, within MPMX19_01045 at 1086.004 kb on + strand, within MPMX19_01045 at 1086.004 kb on + strand, within MPMX19_01045 at 1086.004 kb on + strand, within MPMX19_01045 at 1086.005 kb on - strand, within MPMX19_01045 at 1086.005 kb on - strand, within MPMX19_01045 at 1086.005 kb on - strand, within MPMX19_01045 at 1086.005 kb on - strand, within MPMX19_01045 at 1086.005 kb on - strand, within MPMX19_01045 at 1086.005 kb on - strand, within MPMX19_01045 at 1086.067 kb on + strand, within MPMX19_01045 at 1086.067 kb on + strand, within MPMX19_01045 at 1086.067 kb on + strand, within MPMX19_01045 at 1086.067 kb on + strand, within MPMX19_01045 at 1086.067 kb on + strand, within MPMX19_01045 at 1086.067 kb on + strand, within MPMX19_01045 at 1086.067 kb on + strand, within MPMX19_01045 at 1086.067 kb on + strand, within MPMX19_01045 at 1086.068 kb on - strand, within MPMX19_01045 at 1086.068 kb on - strand, within MPMX19_01045 at 1086.068 kb on - strand, within MPMX19_01045 at 1086.068 kb on - strand, within MPMX19_01045 at 1086.068 kb on - strand, within MPMX19_01045 at 1086.068 kb on - strand, within MPMX19_01045 at 1086.068 kb on - strand, within MPMX19_01045 at 1086.068 kb on - strand, within MPMX19_01045 at 1086.190 kb on + strand, within MPMX19_01045 at 1086.191 kb on - strand, within MPMX19_01045 at 1086.196 kb on + strand, within MPMX19_01045 at 1086.196 kb on + strand, within MPMX19_01045 at 1086.196 kb on + strand, within MPMX19_01045 at 1086.196 kb on + strand, within MPMX19_01045 at 1086.196 kb on + strand, within MPMX19_01045 at 1086.196 kb on + strand, within MPMX19_01045 at 1086.196 kb on + strand, within MPMX19_01045 at 1086.196 kb on + strand, within MPMX19_01045 at 1086.196 kb on + strand, within MPMX19_01045 at 1086.196 kb on + strand, within MPMX19_01045 at 1086.196 kb on + strand, within MPMX19_01045 at 1086.197 kb on - strand, within MPMX19_01045 at 1086.197 kb on - strand, within MPMX19_01045 at 1086.197 kb on - strand, within MPMX19_01045 at 1086.197 kb on - strand, within MPMX19_01045 at 1086.197 kb on - strand, within MPMX19_01045 at 1086.197 kb on - strand, within MPMX19_01045 at 1086.197 kb on - strand, within MPMX19_01045 at 1086.197 kb on - strand, within MPMX19_01045 at 1086.235 kb on + strand at 1086.235 kb on + strand at 1086.235 kb on + strand at 1086.235 kb on + strand at 1086.236 kb on - strand at 1086.236 kb on - strand at 1086.236 kb on - strand at 1086.369 kb on - strand at 1086.369 kb on - strand at 1086.463 kb on - strand at 1086.463 kb on - strand at 1086.566 kb on + strand at 1086.566 kb on + strand at 1086.567 kb on - strand at 1086.626 kb on - strand at 1086.639 kb on + strand at 1086.639 kb on + strand at 1086.639 kb on + strand at 1086.639 kb on + strand at 1086.639 kb on + strand at 1086.639 kb on + strand at 1086.639 kb on + strand at 1086.639 kb on + strand at 1086.639 kb on + strand at 1086.639 kb on + strand at 1086.640 kb on - strand at 1086.640 kb on - strand at 1086.640 kb on - strand at 1086.640 kb on - strand at 1086.640 kb on - strand at 1086.640 kb on - strand at 1086.640 kb on - strand at 1087.240 kb on + strand, within MPMX19_01046 at 1087.240 kb on + strand, within MPMX19_01046 at 1087.240 kb on + strand, within MPMX19_01046 at 1087.240 kb on + strand, within MPMX19_01046 at 1087.240 kb on + strand, within MPMX19_01046 at 1087.240 kb on + strand, within MPMX19_01046 at 1087.240 kb on + strand, within MPMX19_01046 at 1087.240 kb on + strand, within MPMX19_01046 at 1087.240 kb on + strand, within MPMX19_01046 at 1087.240 kb on + strand, within MPMX19_01046 at 1087.240 kb on + strand, within MPMX19_01046 at 1087.240 kb on + strand, within MPMX19_01046 at 1087.240 kb on + strand, within MPMX19_01046 at 1087.240 kb on + strand, within MPMX19_01046 at 1087.240 kb on + strand, within MPMX19_01046 at 1087.240 kb on + strand, within MPMX19_01046 at 1087.240 kb on + strand, within MPMX19_01046 at 1087.240 kb on + strand, within MPMX19_01046 at 1087.240 kb on + strand, within MPMX19_01046 at 1087.240 kb on + strand, within MPMX19_01046 at 1087.240 kb on + strand, within MPMX19_01046 at 1087.240 kb on + strand, within MPMX19_01046 at 1087.276 kb on + strand, within MPMX19_01046 at 1087.276 kb on + strand, within MPMX19_01046 at 1087.276 kb on + strand, within MPMX19_01046 at 1087.276 kb on + strand, within MPMX19_01046 at 1087.276 kb on + strand, within MPMX19_01046 at 1087.276 kb on + strand, within MPMX19_01046 at 1087.276 kb on + strand, within MPMX19_01046 at 1087.277 kb on - strand, within MPMX19_01046 at 1087.277 kb on - strand, within MPMX19_01046 at 1087.277 kb on - strand, within MPMX19_01046 at 1087.277 kb on - strand, within MPMX19_01046 at 1087.277 kb on - strand, within MPMX19_01046 at 1087.531 kb on + strand at 1087.532 kb on - strand at 1087.659 kb on + strand at 1087.659 kb on + strand at 1087.659 kb on + strand at 1087.659 kb on + strand at 1087.660 kb on - strand at 1087.660 kb on - strand at 1087.660 kb on - strand at 1087.660 kb on - strand at 1087.692 kb on + strand at 1087.692 kb on + strand at 1087.693 kb on - strand at 1087.693 kb on - strand at 1087.743 kb on + strand at 1087.743 kb on + strand at 1087.743 kb on + strand at 1087.743 kb on + strand at 1087.743 kb on + strand at 1087.744 kb on - strand at 1087.744 kb on - strand at 1087.744 kb on - strand at 1087.761 kb on - strand at 1087.761 kb on - strand at 1087.776 kb on + strand at 1087.776 kb on + strand at 1087.776 kb on + strand at 1087.776 kb on + strand at 1087.776 kb on + strand at 1087.787 kb on + strand at 1087.788 kb on - strand at 1087.788 kb on - strand at 1087.852 kb on + strand at 1087.852 kb on + strand at 1087.852 kb on + strand at 1087.853 kb on - strand at 1087.854 kb on + strand at 1087.854 kb on + strand at 1087.906 kb on + strand at 1087.907 kb on - strand at 1087.936 kb on - strand at 1087.936 kb on - strand at 1087.974 kb on + strand at 1087.974 kb on + strand at 1087.974 kb on + strand at 1087.975 kb on - strand at 1087.975 kb on - strand at 1087.975 kb on - strand at 1087.975 kb on - strand at 1087.995 kb on + strand at 1088.061 kb on + strand at 1088.061 kb on + strand at 1088.061 kb on + strand at 1088.062 kb on - strand at 1088.062 kb on - strand at 1088.062 kb on - strand at 1088.062 kb on - strand at 1088.062 kb on - strand at 1088.086 kb on + strand at 1088.086 kb on + strand at 1088.086 kb on + strand at 1088.086 kb on + strand at 1088.086 kb on + strand at 1088.087 kb on - strand at 1088.087 kb on - strand at 1088.087 kb on - strand at 1088.087 kb on - strand at 1088.087 kb on - strand at 1088.087 kb on - strand at 1088.087 kb on - strand at 1088.087 kb on - strand at 1088.087 kb on - strand at 1088.113 kb on - strand at 1088.123 kb on + strand at 1088.123 kb on + strand at 1088.123 kb on + strand at 1088.123 kb on + strand at 1088.123 kb on + strand at 1088.123 kb on + strand at 1088.123 kb on + strand at 1088.124 kb on - strand at 1088.124 kb on - strand at 1088.124 kb on - strand at 1088.124 kb on - strand at 1088.124 kb on - strand at 1088.124 kb on - strand at 1088.166 kb on + strand at 1088.296 kb on - strand at 1088.361 kb on + strand, within MPMX19_01047 at 1088.361 kb on + strand, within MPMX19_01047 at 1088.361 kb on + strand, within MPMX19_01047 at 1088.361 kb on + strand, within MPMX19_01047 at 1088.361 kb on + strand, within MPMX19_01047 at 1088.361 kb on + strand, within MPMX19_01047 at 1088.362 kb on - strand, within MPMX19_01047 at 1088.362 kb on - strand, within MPMX19_01047 at 1088.362 kb on - strand, within MPMX19_01047 at 1088.362 kb on - strand, within MPMX19_01047 at 1088.362 kb on - strand, within MPMX19_01047 at 1088.383 kb on - strand, within MPMX19_01047 at 1088.433 kb on + strand, within MPMX19_01047 at 1088.433 kb on + strand, within MPMX19_01047 at 1088.434 kb on - strand, within MPMX19_01047
Per-strain Table
Position Strand Gene LocusTag Fraction NL-CCM_agar remove 1,085,858 + MPMX19_01045 0.65 +0.2 1,085,859 - MPMX19_01045 0.65 +0.6 1,085,859 - MPMX19_01045 0.65 +0.5 1,085,859 - MPMX19_01045 0.65 +0.1 1,085,859 - MPMX19_01045 0.65 +0.3 1,085,860 + MPMX19_01045 0.65 +0.7 1,085,860 + MPMX19_01045 0.65 +0.2 1,085,860 + MPMX19_01045 0.65 +1.4 1,085,860 + MPMX19_01045 0.65 +0.7 1,085,860 + MPMX19_01045 0.65 +0.6 1,085,860 + MPMX19_01045 0.65 +0.6 1,085,860 + MPMX19_01045 0.65 +1.6 1,085,860 + MPMX19_01045 0.65 +0.5 1,085,860 + MPMX19_01045 0.65 -0.2 1,085,860 + MPMX19_01045 0.65 +0.5 1,085,860 + MPMX19_01045 0.65 +1.5 1,085,860 + MPMX19_01045 0.65 -1.8 1,085,860 + MPMX19_01045 0.65 +0.3 1,085,860 + MPMX19_01045 0.65 -0.1 1,085,860 + MPMX19_01045 0.65 +1.6 1,085,860 + MPMX19_01045 0.65 +0.6 1,085,861 - MPMX19_01045 0.65 +0.8 1,085,861 - MPMX19_01045 0.65 +0.6 1,085,861 - MPMX19_01045 0.65 +0.2 1,085,861 - MPMX19_01045 0.65 +0.0 1,085,861 - MPMX19_01045 0.65 -0.3 1,085,861 - MPMX19_01045 0.65 +0.7 1,085,861 - MPMX19_01045 0.65 -0.8 1,085,861 - MPMX19_01045 0.65 +1.5 1,085,861 - MPMX19_01045 0.65 +1.1 1,085,861 - MPMX19_01045 0.65 +2.1 1,085,861 - MPMX19_01045 0.65 +0.6 1,085,861 - MPMX19_01045 0.65 +0.6 1,085,992 + MPMX19_01045 0.75 +0.9 1,085,992 + MPMX19_01045 0.75 +0.8 1,085,992 + MPMX19_01045 0.75 +0.5 1,085,992 + MPMX19_01045 0.75 +1.8 1,085,993 - MPMX19_01045 0.75 +0.5 1,085,993 - MPMX19_01045 0.75 +1.3 1,085,993 - MPMX19_01045 0.75 +0.5 1,085,993 - MPMX19_01045 0.75 +0.6 1,086,004 + MPMX19_01045 0.76 -1.2 1,086,004 + MPMX19_01045 0.76 +0.9 1,086,004 + MPMX19_01045 0.76 -0.8 1,086,004 + MPMX19_01045 0.76 +1.2 1,086,004 + MPMX19_01045 0.76 +0.6 1,086,005 - MPMX19_01045 0.76 +1.1 1,086,005 - MPMX19_01045 0.76 +0.4 1,086,005 - MPMX19_01045 0.76 -0.1 1,086,005 - MPMX19_01045 0.76 -0.4 1,086,005 - MPMX19_01045 0.76 -0.6 1,086,005 - MPMX19_01045 0.76 -0.4 1,086,067 + MPMX19_01045 0.80 -0.5 1,086,067 + MPMX19_01045 0.80 +0.1 1,086,067 + MPMX19_01045 0.80 +1.2 1,086,067 + MPMX19_01045 0.80 -0.1 1,086,067 + MPMX19_01045 0.80 -1.3 1,086,067 + MPMX19_01045 0.80 -0.5 1,086,067 + MPMX19_01045 0.80 -1.1 1,086,067 + MPMX19_01045 0.80 +0.2 1,086,068 - MPMX19_01045 0.80 +0.8 1,086,068 - MPMX19_01045 0.80 -1.3 1,086,068 - MPMX19_01045 0.80 -0.5 1,086,068 - MPMX19_01045 0.80 +0.2 1,086,068 - MPMX19_01045 0.80 +0.3 1,086,068 - MPMX19_01045 0.80 +0.0 1,086,068 - MPMX19_01045 0.80 +0.2 1,086,068 - MPMX19_01045 0.80 +1.4 1,086,190 + MPMX19_01045 0.89 +0.0 1,086,191 - MPMX19_01045 0.89 +0.1 1,086,196 + MPMX19_01045 0.90 +0.9 1,086,196 + MPMX19_01045 0.90 -0.5 1,086,196 + MPMX19_01045 0.90 +0.9 1,086,196 + MPMX19_01045 0.90 +1.8 1,086,196 + MPMX19_01045 0.90 -0.3 1,086,196 + MPMX19_01045 0.90 +0.5 1,086,196 + MPMX19_01045 0.90 +1.5 1,086,196 + MPMX19_01045 0.90 +0.1 1,086,196 + MPMX19_01045 0.90 +0.0 1,086,196 + MPMX19_01045 0.90 +1.1 1,086,196 + MPMX19_01045 0.90 +1.7 1,086,197 - MPMX19_01045 0.90 -0.5 1,086,197 - MPMX19_01045 0.90 +0.2 1,086,197 - MPMX19_01045 0.90 +0.2 1,086,197 - MPMX19_01045 0.90 -1.6 1,086,197 - MPMX19_01045 0.90 -1.2 1,086,197 - MPMX19_01045 0.90 +0.8 1,086,197 - MPMX19_01045 0.90 +0.7 1,086,197 - MPMX19_01045 0.90 -2.2 1,086,235 + -0.1 1,086,235 + -0.5 1,086,235 + -1.5 1,086,235 + +0.8 1,086,236 - +0.3 1,086,236 - -1.2 1,086,236 - +0.6 1,086,369 - -0.2 1,086,369 - -2.1 1,086,463 - -1.7 1,086,463 - +0.3 1,086,566 + -0.2 1,086,566 + -0.5 1,086,567 - +0.4 1,086,626 - +0.9 1,086,639 + +1.5 1,086,639 + -2.4 1,086,639 + -0.2 1,086,639 + +0.3 1,086,639 + +0.7 1,086,639 + -0.7 1,086,639 + +0.0 1,086,639 + -0.9 1,086,639 + -1.7 1,086,639 + +0.2 1,086,640 - -1.7 1,086,640 - +1.1 1,086,640 - -1.2 1,086,640 - -0.5 1,086,640 - -0.6 1,086,640 - -1.4 1,086,640 - -1.1 1,087,240 + MPMX19_01046 0.62 +0.3 1,087,240 + MPMX19_01046 0.62 -0.6 1,087,240 + MPMX19_01046 0.62 -0.6 1,087,240 + MPMX19_01046 0.62 -0.2 1,087,240 + MPMX19_01046 0.62 -0.5 1,087,240 + MPMX19_01046 0.62 +1.3 1,087,240 + MPMX19_01046 0.62 -0.4 1,087,240 + MPMX19_01046 0.62 -1.5 1,087,240 + MPMX19_01046 0.62 -0.7 1,087,240 + MPMX19_01046 0.62 +0.2 1,087,240 + MPMX19_01046 0.62 -2.9 1,087,240 + MPMX19_01046 0.62 -1.1 1,087,240 + MPMX19_01046 0.62 +0.3 1,087,240 + MPMX19_01046 0.62 +1.0 1,087,240 + MPMX19_01046 0.62 -2.6 1,087,240 + MPMX19_01046 0.62 -1.1 1,087,240 + MPMX19_01046 0.62 +0.1 1,087,240 + MPMX19_01046 0.62 -0.6 1,087,240 + MPMX19_01046 0.62 +0.2 1,087,240 + MPMX19_01046 0.62 -0.3 1,087,240 + MPMX19_01046 0.62 +0.0 1,087,240 + MPMX19_01046 0.62 -0.5 1,087,276 + MPMX19_01046 0.66 +0.0 1,087,276 + MPMX19_01046 0.66 -0.4 1,087,276 + MPMX19_01046 0.66 -0.5 1,087,276 + MPMX19_01046 0.66 -0.2 1,087,276 + MPMX19_01046 0.66 -0.1 1,087,276 + MPMX19_01046 0.66 +0.3 1,087,276 + MPMX19_01046 0.66 -1.3 1,087,277 - MPMX19_01046 0.66 -0.2 1,087,277 - MPMX19_01046 0.66 -0.6 1,087,277 - MPMX19_01046 0.66 -1.1 1,087,277 - MPMX19_01046 0.66 -0.7 1,087,277 - MPMX19_01046 0.66 -0.7 1,087,531 + +0.5 1,087,532 - -1.2 1,087,659 + -0.8 1,087,659 + -2.0 1,087,659 + +0.1 1,087,659 + +1.2 1,087,660 - -0.6 1,087,660 - +1.1 1,087,660 - -0.2 1,087,660 - +0.2 1,087,692 + +0.0 1,087,692 + -1.7 1,087,693 - -0.4 1,087,693 - -0.4 1,087,743 + +0.2 1,087,743 + -1.3 1,087,743 + -1.3 1,087,743 + -1.3 1,087,743 + -0.0 1,087,744 - +0.6 1,087,744 - -0.6 1,087,744 - +0.5 1,087,761 - +0.5 1,087,761 - +0.7 1,087,776 + +0.3 1,087,776 + -0.5 1,087,776 + +0.3 1,087,776 + +0.3 1,087,776 + -0.9 1,087,787 + -0.8 1,087,788 - +0.3 1,087,788 - +0.6 1,087,852 + -0.1 1,087,852 + +1.3 1,087,852 + -0.8 1,087,853 - +0.9 1,087,854 + +0.3 1,087,854 + +0.2 1,087,906 + +0.2 1,087,907 - +0.1 1,087,936 - +0.2 1,087,936 - -0.8 1,087,974 + -0.4 1,087,974 + -0.1 1,087,974 + -1.2 1,087,975 - +1.8 1,087,975 - +1.5 1,087,975 - +0.3 1,087,975 - -0.3 1,087,995 + +0.4 1,088,061 + -0.3 1,088,061 + +0.5 1,088,061 + -0.5 1,088,062 - +0.6 1,088,062 - +0.5 1,088,062 - -0.6 1,088,062 - -1.5 1,088,062 - -0.7 1,088,086 + -0.2 1,088,086 + -0.3 1,088,086 + -0.5 1,088,086 + -0.0 1,088,086 + -0.9 1,088,087 - +0.0 1,088,087 - -1.5 1,088,087 - -0.7 1,088,087 - +0.5 1,088,087 - -0.7 1,088,087 - +2.0 1,088,087 - +0.3 1,088,087 - +0.8 1,088,087 - -0.7 1,088,113 - +0.5 1,088,123 + -0.3 1,088,123 + +1.1 1,088,123 + -2.6 1,088,123 + -1.7 1,088,123 + -0.7 1,088,123 + -0.7 1,088,123 + -0.2 1,088,124 - +0.8 1,088,124 - -2.2 1,088,124 - -1.8 1,088,124 - -0.5 1,088,124 - +0.1 1,088,124 - -1.8 1,088,166 + -1.4 1,088,296 - -0.7 1,088,361 + MPMX19_01047 0.16 +0.5 1,088,361 + MPMX19_01047 0.16 +1.2 1,088,361 + MPMX19_01047 0.16 +0.3 1,088,361 + MPMX19_01047 0.16 -0.1 1,088,361 + MPMX19_01047 0.16 +1.0 1,088,361 + MPMX19_01047 0.16 +0.5 1,088,362 - MPMX19_01047 0.17 -0.1 1,088,362 - MPMX19_01047 0.17 -0.0 1,088,362 - MPMX19_01047 0.17 +0.2 1,088,362 - MPMX19_01047 0.17 -0.8 1,088,362 - MPMX19_01047 0.17 -0.2 1,088,383 - MPMX19_01047 0.22 +1.1 1,088,433 + MPMX19_01047 0.34 +0.3 1,088,433 + MPMX19_01047 0.34 +1.2 1,088,434 - MPMX19_01047 0.34 -1.0
Or see this region's nucleotide sequence