Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF977 and GFF978 are separated by 105 nucleotides GFF978 and GFF979 are separated by 25 nucleotides GFF979 and GFF980 are separated by 101 nucleotides
GFF977 - Protein MgtC, at 208,571 to 209,293
GFF977
GFF978 - Phosphate import ATP-binding protein PstB, at 209,399 to 210,178
GFF978
GFF979 - hypothetical protein, at 210,204 to 211,142
GFF979
GFF980 - hypothetical protein, at 211,244 to 212,170
GFF980
Position (kb)
210
211
212 Strain fitness (log2 ratio)
-2
-1
0
1 at 209.252 kb on + strand at 209.252 kb on + strand at 209.252 kb on + strand at 209.252 kb on + strand at 209.252 kb on + strand at 209.253 kb on - strand at 209.253 kb on - strand at 209.256 kb on + strand at 209.256 kb on + strand at 209.256 kb on + strand at 209.256 kb on + strand at 209.256 kb on + strand at 209.256 kb on + strand at 209.257 kb on - strand at 209.257 kb on - strand at 209.257 kb on - strand at 209.257 kb on - strand at 209.257 kb on - strand at 209.257 kb on - strand at 209.292 kb on - strand at 209.295 kb on + strand at 209.296 kb on - strand at 209.385 kb on + strand at 209.385 kb on + strand at 209.387 kb on + strand at 209.401 kb on - strand at 209.401 kb on - strand at 209.401 kb on - strand at 210.206 kb on - strand at 210.206 kb on - strand at 210.231 kb on - strand at 211.191 kb on - strand at 211.191 kb on - strand at 211.191 kb on - strand at 211.191 kb on - strand at 211.202 kb on - strand at 211.202 kb on - strand at 211.213 kb on - strand at 211.213 kb on - strand at 211.231 kb on + strand at 211.232 kb on - strand at 211.232 kb on - strand at 211.232 kb on - strand at 211.248 kb on + strand at 211.249 kb on - strand at 211.249 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 209,252 + -2.6 209,252 + +0.3 209,252 + -0.1 209,252 + -0.2 209,252 + -1.0 209,253 - -0.3 209,253 - -0.7 209,256 + +0.4 209,256 + -1.3 209,256 + +0.0 209,256 + -1.2 209,256 + -2.0 209,256 + +0.7 209,257 - -1.0 209,257 - +0.0 209,257 - -0.7 209,257 - -0.3 209,257 - +0.3 209,257 - -1.9 209,292 - -2.3 209,295 + -1.5 209,296 - -1.3 209,385 + -1.3 209,385 + +0.6 209,387 + +0.0 209,401 - -0.8 209,401 - -0.7 209,401 - -0.5 210,206 - +1.0 210,206 - -1.0 210,231 - -1.0 211,191 - -2.2 211,191 - -0.9 211,191 - -1.3 211,191 - -1.3 211,202 - -0.3 211,202 - -0.3 211,213 - -0.2 211,213 - -0.7 211,231 + -2.6 211,232 - +0.6 211,232 - +0.0 211,232 - -0.3 211,248 + -1.0 211,249 - +0.0 211,249 - -1.0
Or see this region's nucleotide sequence