Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF69 and GFF70 are separated by 0 nucleotides GFF70 and GFF71 are separated by 140 nucleotides GFF71 and GFF72 are separated by 340 nucleotides
GFF69 - Phosphomannomutase/phosphoglucomutase, at 78,278 to 79,684
GFF69
GFF70 - Protein IscX, at 79,685 to 79,879
GFF70
GFF71 - hypothetical protein, at 80,020 to 82,059
GFF71
GFF72 - Serine hydroxymethyltransferase, at 82,400 to 83,647
GFF72
Position (kb)
80
81
82
83 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 79.104 kb on - strand, within GFF69 at 79.212 kb on + strand, within GFF69 at 79.221 kb on + strand, within GFF69 at 79.221 kb on + strand, within GFF69 at 79.222 kb on - strand, within GFF69 at 79.222 kb on - strand, within GFF69 at 79.222 kb on - strand, within GFF69 at 79.225 kb on + strand, within GFF69 at 79.225 kb on + strand, within GFF69 at 79.226 kb on - strand, within GFF69 at 79.226 kb on - strand, within GFF69 at 79.230 kb on + strand, within GFF69 at 79.231 kb on - strand, within GFF69 at 79.231 kb on - strand, within GFF69 at 79.231 kb on - strand, within GFF69 at 79.231 kb on - strand, within GFF69 at 79.231 kb on - strand, within GFF69 at 79.264 kb on - strand, within GFF69 at 79.342 kb on + strand, within GFF69 at 79.353 kb on + strand, within GFF69 at 79.353 kb on + strand, within GFF69 at 79.354 kb on - strand, within GFF69 at 79.354 kb on - strand, within GFF69 at 79.354 kb on - strand, within GFF69 at 79.354 kb on - strand, within GFF69 at 79.354 kb on - strand, within GFF69 at 79.354 kb on - strand, within GFF69 at 79.367 kb on - strand, within GFF69 at 79.410 kb on + strand, within GFF69 at 79.410 kb on + strand, within GFF69 at 79.410 kb on + strand, within GFF69 at 79.411 kb on - strand, within GFF69 at 79.425 kb on + strand, within GFF69 at 79.425 kb on + strand, within GFF69 at 79.425 kb on + strand, within GFF69 at 79.534 kb on - strand, within GFF69 at 79.549 kb on + strand at 79.549 kb on + strand at 79.549 kb on + strand at 79.549 kb on + strand at 79.550 kb on - strand at 79.550 kb on - strand at 79.550 kb on - strand at 79.648 kb on - strand at 79.648 kb on - strand at 79.687 kb on - strand at 79.700 kb on + strand at 79.839 kb on + strand, within GFF70 at 79.839 kb on + strand, within GFF70 at 79.839 kb on + strand, within GFF70 at 79.839 kb on + strand, within GFF70 at 79.840 kb on - strand, within GFF70 at 79.840 kb on - strand, within GFF70 at 79.840 kb on - strand, within GFF70 at 79.840 kb on - strand, within GFF70 at 79.919 kb on + strand at 79.919 kb on + strand at 79.920 kb on - strand at 79.921 kb on + strand at 79.921 kb on + strand at 79.921 kb on + strand at 79.922 kb on - strand at 79.931 kb on + strand at 79.931 kb on + strand at 79.931 kb on + strand at 79.931 kb on + strand at 79.931 kb on + strand at 79.932 kb on - strand at 79.932 kb on - strand at 79.932 kb on - strand at 79.932 kb on - strand at 79.958 kb on + strand at 80.019 kb on + strand at 80.019 kb on + strand at 80.028 kb on + strand at 80.028 kb on + strand at 80.029 kb on - strand at 80.034 kb on + strand at 80.035 kb on - strand at 80.035 kb on - strand at 80.081 kb on + strand at 80.081 kb on + strand at 80.081 kb on + strand at 80.081 kb on + strand at 80.081 kb on + strand at 80.081 kb on + strand at 80.081 kb on + strand at 80.082 kb on - strand at 80.082 kb on - strand at 80.082 kb on - strand at 80.082 kb on - strand at 80.082 kb on - strand at 80.100 kb on - strand at 80.100 kb on - strand at 80.100 kb on - strand at 80.100 kb on - strand at 80.100 kb on - strand at 80.171 kb on + strand at 80.171 kb on + strand at 80.189 kb on + strand at 80.190 kb on - strand at 80.238 kb on - strand, within GFF71 at 80.238 kb on - strand, within GFF71 at 80.238 kb on - strand, within GFF71 at 80.387 kb on + strand, within GFF71 at 80.387 kb on + strand, within GFF71 at 80.387 kb on + strand, within GFF71 at 80.395 kb on + strand, within GFF71 at 80.395 kb on + strand, within GFF71 at 80.395 kb on + strand, within GFF71 at 80.395 kb on + strand, within GFF71 at 80.395 kb on + strand, within GFF71 at 80.396 kb on - strand, within GFF71 at 80.396 kb on - strand, within GFF71 at 80.396 kb on - strand, within GFF71 at 80.396 kb on - strand, within GFF71 at 80.396 kb on - strand, within GFF71 at 80.396 kb on - strand, within GFF71 at 80.396 kb on - strand, within GFF71 at 80.396 kb on - strand, within GFF71 at 80.396 kb on - strand, within GFF71 at 80.557 kb on - strand, within GFF71 at 80.577 kb on + strand, within GFF71 at 80.660 kb on + strand, within GFF71 at 80.698 kb on - strand, within GFF71 at 80.698 kb on - strand, within GFF71 at 80.698 kb on - strand, within GFF71 at 80.719 kb on + strand, within GFF71 at 80.719 kb on + strand, within GFF71 at 80.719 kb on + strand, within GFF71 at 80.719 kb on + strand, within GFF71 at 80.719 kb on + strand, within GFF71 at 80.720 kb on - strand, within GFF71 at 80.720 kb on - strand, within GFF71 at 80.720 kb on - strand, within GFF71 at 80.720 kb on - strand, within GFF71 at 80.720 kb on - strand, within GFF71 at 80.753 kb on + strand, within GFF71 at 80.753 kb on + strand, within GFF71 at 80.753 kb on + strand, within GFF71 at 80.754 kb on - strand, within GFF71 at 80.754 kb on - strand, within GFF71 at 80.754 kb on - strand, within GFF71 at 80.754 kb on - strand, within GFF71 at 80.755 kb on + strand, within GFF71 at 80.755 kb on + strand, within GFF71 at 80.755 kb on + strand, within GFF71 at 80.755 kb on + strand, within GFF71 at 80.756 kb on - strand, within GFF71 at 80.792 kb on + strand, within GFF71 at 80.793 kb on - strand, within GFF71 at 80.798 kb on + strand, within GFF71 at 80.799 kb on - strand, within GFF71 at 81.093 kb on - strand, within GFF71 at 81.093 kb on - strand, within GFF71 at 81.093 kb on - strand, within GFF71 at 81.124 kb on + strand, within GFF71 at 81.124 kb on + strand, within GFF71 at 81.124 kb on + strand, within GFF71 at 81.124 kb on + strand, within GFF71 at 81.124 kb on + strand, within GFF71 at 81.125 kb on - strand, within GFF71 at 81.125 kb on - strand, within GFF71 at 81.156 kb on + strand, within GFF71 at 81.157 kb on - strand, within GFF71 at 81.157 kb on - strand, within GFF71 at 81.157 kb on - strand, within GFF71 at 81.174 kb on - strand, within GFF71 at 81.207 kb on + strand, within GFF71 at 81.207 kb on + strand, within GFF71 at 81.207 kb on + strand, within GFF71 at 81.207 kb on + strand, within GFF71 at 81.208 kb on - strand, within GFF71 at 81.239 kb on + strand, within GFF71 at 81.239 kb on + strand, within GFF71 at 81.239 kb on + strand, within GFF71 at 81.239 kb on + strand, within GFF71 at 81.240 kb on - strand, within GFF71 at 81.240 kb on - strand, within GFF71 at 81.240 kb on - strand, within GFF71 at 81.240 kb on - strand, within GFF71 at 81.264 kb on + strand, within GFF71 at 81.399 kb on - strand, within GFF71 at 81.399 kb on - strand, within GFF71 at 81.475 kb on + strand, within GFF71 at 81.476 kb on - strand, within GFF71 at 81.620 kb on + strand, within GFF71 at 81.620 kb on + strand, within GFF71 at 81.741 kb on + strand, within GFF71 at 81.741 kb on + strand, within GFF71 at 81.742 kb on - strand, within GFF71 at 81.823 kb on - strand, within GFF71 at 81.823 kb on - strand, within GFF71 at 81.855 kb on + strand, within GFF71 at 81.855 kb on + strand, within GFF71 at 81.855 kb on + strand, within GFF71 at 81.856 kb on - strand at 81.856 kb on - strand at 81.938 kb on + strand at 81.938 kb on + strand at 81.939 kb on - strand at 81.939 kb on - strand at 82.005 kb on + strand at 82.006 kb on - strand at 82.006 kb on - strand at 82.013 kb on + strand at 82.013 kb on + strand at 82.013 kb on + strand at 82.013 kb on + strand at 82.014 kb on - strand at 82.014 kb on - strand at 82.014 kb on - strand at 82.014 kb on - strand at 82.014 kb on - strand at 82.132 kb on + strand at 82.132 kb on + strand at 82.133 kb on - strand at 82.133 kb on - strand at 82.134 kb on + strand at 82.134 kb on + strand at 82.134 kb on + strand at 82.134 kb on + strand at 82.135 kb on - strand at 82.135 kb on - strand at 82.135 kb on - strand at 82.135 kb on - strand at 82.135 kb on - strand at 82.153 kb on - strand at 82.159 kb on - strand at 82.200 kb on + strand at 82.201 kb on - strand at 82.201 kb on - strand at 82.201 kb on - strand at 82.262 kb on + strand at 82.263 kb on - strand at 82.263 kb on - strand at 82.298 kb on + strand at 82.298 kb on + strand at 82.299 kb on - strand at 82.306 kb on + strand at 82.307 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 79,104 - GFF69 0.59 +0.6 79,212 + GFF69 0.66 +0.3 79,221 + GFF69 0.67 +0.1 79,221 + GFF69 0.67 -1.2 79,222 - GFF69 0.67 +1.0 79,222 - GFF69 0.67 -0.7 79,222 - GFF69 0.67 -1.6 79,225 + GFF69 0.67 +0.0 79,225 + GFF69 0.67 +0.0 79,226 - GFF69 0.67 -1.3 79,226 - GFF69 0.67 -1.1 79,230 + GFF69 0.68 -1.0 79,231 - GFF69 0.68 -1.0 79,231 - GFF69 0.68 -1.6 79,231 - GFF69 0.68 -0.1 79,231 - GFF69 0.68 -0.7 79,231 - GFF69 0.68 -0.1 79,264 - GFF69 0.70 -0.2 79,342 + GFF69 0.76 -0.1 79,353 + GFF69 0.76 -0.5 79,353 + GFF69 0.76 +0.6 79,354 - GFF69 0.76 +0.3 79,354 - GFF69 0.76 -0.5 79,354 - GFF69 0.76 +1.3 79,354 - GFF69 0.76 +0.0 79,354 - GFF69 0.76 -1.6 79,354 - GFF69 0.76 -0.4 79,367 - GFF69 0.77 -0.8 79,410 + GFF69 0.80 +0.1 79,410 + GFF69 0.80 -0.1 79,410 + GFF69 0.80 +1.8 79,411 - GFF69 0.81 -1.0 79,425 + GFF69 0.82 +0.8 79,425 + GFF69 0.82 -0.6 79,425 + GFF69 0.82 -0.8 79,534 - GFF69 0.89 +1.3 79,549 + +0.7 79,549 + -0.3 79,549 + -0.3 79,549 + +0.0 79,550 - -0.6 79,550 - -0.4 79,550 - +0.6 79,648 - -1.0 79,648 - -0.6 79,687 - -0.2 79,700 + -1.8 79,839 + GFF70 0.79 +0.4 79,839 + GFF70 0.79 -1.8 79,839 + GFF70 0.79 -1.0 79,839 + GFF70 0.79 +0.4 79,840 - GFF70 0.79 +0.4 79,840 - GFF70 0.79 +0.0 79,840 - GFF70 0.79 -1.5 79,840 - GFF70 0.79 -0.5 79,919 + -1.0 79,919 + -0.0 79,920 - -1.8 79,921 + -0.2 79,921 + -1.3 79,921 + -0.6 79,922 - -0.3 79,931 + -1.0 79,931 + -2.0 79,931 + -2.0 79,931 + +1.3 79,931 + -0.9 79,932 - -0.8 79,932 - -0.2 79,932 - +0.6 79,932 - -1.7 79,958 + -0.4 80,019 + -1.0 80,019 + -0.3 80,028 + +0.2 80,028 + -0.1 80,029 - +0.6 80,034 + -0.2 80,035 - +0.0 80,035 - +1.3 80,081 + -0.8 80,081 + -0.3 80,081 + -1.3 80,081 + +0.0 80,081 + -1.9 80,081 + +0.2 80,081 + -0.8 80,082 - -2.6 80,082 - -0.6 80,082 - -0.2 80,082 - -1.3 80,082 - +0.1 80,100 - -0.2 80,100 - -0.9 80,100 - -1.2 80,100 - -0.3 80,100 - +0.7 80,171 + -1.4 80,171 + -1.7 80,189 + +0.0 80,190 - +0.2 80,238 - GFF71 0.11 -0.7 80,238 - GFF71 0.11 -0.3 80,238 - GFF71 0.11 -0.4 80,387 + GFF71 0.18 -1.0 80,387 + GFF71 0.18 -1.0 80,387 + GFF71 0.18 +0.7 80,395 + GFF71 0.18 -0.2 80,395 + GFF71 0.18 +0.0 80,395 + GFF71 0.18 -1.2 80,395 + GFF71 0.18 -0.0 80,395 + GFF71 0.18 -0.4 80,396 - GFF71 0.18 -1.0 80,396 - GFF71 0.18 -1.0 80,396 - GFF71 0.18 -0.2 80,396 - GFF71 0.18 -0.4 80,396 - GFF71 0.18 -2.3 80,396 - GFF71 0.18 -1.3 80,396 - GFF71 0.18 +1.0 80,396 - GFF71 0.18 -1.3 80,396 - GFF71 0.18 -0.3 80,557 - GFF71 0.26 -1.1 80,577 + GFF71 0.27 -0.8 80,660 + GFF71 0.31 -0.4 80,698 - GFF71 0.33 +0.6 80,698 - GFF71 0.33 -1.3 80,698 - GFF71 0.33 -0.9 80,719 + GFF71 0.34 -0.8 80,719 + GFF71 0.34 -0.6 80,719 + GFF71 0.34 -0.3 80,719 + GFF71 0.34 +0.2 80,719 + GFF71 0.34 -0.3 80,720 - GFF71 0.34 -0.3 80,720 - GFF71 0.34 -0.3 80,720 - GFF71 0.34 -1.0 80,720 - GFF71 0.34 -0.4 80,720 - GFF71 0.34 -1.3 80,753 + GFF71 0.36 +0.0 80,753 + GFF71 0.36 -0.9 80,753 + GFF71 0.36 -1.7 80,754 - GFF71 0.36 -0.2 80,754 - GFF71 0.36 -0.8 80,754 - GFF71 0.36 -1.3 80,754 - GFF71 0.36 -1.0 80,755 + GFF71 0.36 -0.8 80,755 + GFF71 0.36 +0.3 80,755 + GFF71 0.36 +0.8 80,755 + GFF71 0.36 -1.6 80,756 - GFF71 0.36 -0.3 80,792 + GFF71 0.38 +0.4 80,793 - GFF71 0.38 -0.3 80,798 + GFF71 0.38 +1.0 80,799 - GFF71 0.38 -1.2 81,093 - GFF71 0.53 -1.8 81,093 - GFF71 0.53 +0.4 81,093 - GFF71 0.53 +0.7 81,124 + GFF71 0.54 -1.6 81,124 + GFF71 0.54 -0.6 81,124 + GFF71 0.54 -0.7 81,124 + GFF71 0.54 +0.0 81,124 + GFF71 0.54 -0.6 81,125 - GFF71 0.54 +0.4 81,125 - GFF71 0.54 -0.2 81,156 + GFF71 0.56 +0.0 81,157 - GFF71 0.56 -1.5 81,157 - GFF71 0.56 -0.8 81,157 - GFF71 0.56 -1.3 81,174 - GFF71 0.57 +0.5 81,207 + GFF71 0.58 -1.3 81,207 + GFF71 0.58 -1.3 81,207 + GFF71 0.58 +0.8 81,207 + GFF71 0.58 -2.1 81,208 - GFF71 0.58 +0.6 81,239 + GFF71 0.60 +0.0 81,239 + GFF71 0.60 -0.3 81,239 + GFF71 0.60 +0.6 81,239 + GFF71 0.60 -0.8 81,240 - GFF71 0.60 +0.0 81,240 - GFF71 0.60 -0.6 81,240 - GFF71 0.60 -1.2 81,240 - GFF71 0.60 +0.0 81,264 + GFF71 0.61 +0.6 81,399 - GFF71 0.68 -1.2 81,399 - GFF71 0.68 +0.0 81,475 + GFF71 0.71 +0.6 81,476 - GFF71 0.71 +0.7 81,620 + GFF71 0.78 -0.2 81,620 + GFF71 0.78 -0.3 81,741 + GFF71 0.84 +0.6 81,741 + GFF71 0.84 +0.0 81,742 - GFF71 0.84 +0.2 81,823 - GFF71 0.88 +0.7 81,823 - GFF71 0.88 +0.0 81,855 + GFF71 0.90 -0.5 81,855 + GFF71 0.90 -1.0 81,855 + GFF71 0.90 -0.8 81,856 - -0.8 81,856 - -0.6 81,938 + -0.8 81,938 + +0.2 81,939 - -0.6 81,939 - -1.6 82,005 + -0.3 82,006 - +0.3 82,006 - -0.6 82,013 + -0.4 82,013 + -1.3 82,013 + -1.3 82,013 + -0.1 82,014 - -0.1 82,014 - -1.0 82,014 - -0.8 82,014 - -0.5 82,014 - -0.9 82,132 + -0.9 82,132 + -1.8 82,133 - -1.2 82,133 - -1.3 82,134 + +0.6 82,134 + -1.5 82,134 + -2.6 82,134 + +0.3 82,135 - +0.0 82,135 - -1.1 82,135 - -0.1 82,135 - -0.2 82,135 - -1.6 82,153 - -1.0 82,159 - -1.4 82,200 + +0.0 82,201 - -0.6 82,201 - -0.9 82,201 - -0.2 82,262 + -1.1 82,263 - -0.1 82,263 - +0.2 82,298 + +0.0 82,298 + -1.7 82,299 - -0.3 82,306 + +0.0 82,307 - +0.8
Or see this region's nucleotide sequence