Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF29 and GFF30 are separated by 9 nucleotides GFF30 and GFF31 are separated by 49 nucleotides GFF31 and GFF32 are separated by 122 nucleotides
GFF29 - hypothetical protein, at 40,177 to 41,802
GFF29
GFF30 - hypothetical protein, at 41,812 to 42,486
GFF30
GFF31 - hypothetical protein, at 42,536 to 43,222
GFF31
GFF32 - hypothetical protein, at 43,345 to 44,301
GFF32
Position (kb) 41
42
43 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 40.860 kb on + strand, within GFF29 at 40.894 kb on - strand, within GFF29 at 40.901 kb on - strand, within GFF29 at 40.901 kb on - strand, within GFF29 at 40.917 kb on - strand, within GFF29 at 40.947 kb on + strand, within GFF29 at 41.028 kb on + strand, within GFF29 at 41.028 kb on + strand, within GFF29 at 41.029 kb on - strand, within GFF29 at 41.034 kb on + strand, within GFF29 at 41.035 kb on - strand, within GFF29 at 41.100 kb on + strand, within GFF29 at 41.118 kb on + strand, within GFF29 at 41.119 kb on - strand, within GFF29 at 41.126 kb on + strand, within GFF29 at 41.126 kb on + strand, within GFF29 at 41.126 kb on + strand, within GFF29 at 41.127 kb on - strand, within GFF29 at 41.127 kb on - strand, within GFF29 at 41.127 kb on - strand, within GFF29 at 41.127 kb on - strand, within GFF29 at 41.127 kb on - strand, within GFF29 at 41.128 kb on + strand, within GFF29 at 41.128 kb on + strand, within GFF29 at 41.128 kb on + strand, within GFF29 at 41.128 kb on + strand, within GFF29 at 41.128 kb on + strand, within GFF29 at 41.128 kb on + strand, within GFF29 at 41.129 kb on - strand, within GFF29 at 41.129 kb on - strand, within GFF29 at 41.129 kb on - strand, within GFF29 at 41.129 kb on - strand, within GFF29 at 41.129 kb on - strand, within GFF29 at 41.164 kb on - strand, within GFF29 at 41.172 kb on + strand, within GFF29 at 41.173 kb on - strand, within GFF29 at 41.173 kb on - strand, within GFF29 at 41.194 kb on + strand, within GFF29 at 41.194 kb on + strand, within GFF29 at 41.195 kb on - strand, within GFF29 at 41.195 kb on - strand, within GFF29 at 41.197 kb on + strand, within GFF29 at 41.197 kb on + strand, within GFF29 at 41.198 kb on - strand, within GFF29 at 41.198 kb on - strand, within GFF29 at 41.198 kb on - strand, within GFF29 at 41.198 kb on - strand, within GFF29 at 41.220 kb on + strand, within GFF29 at 41.221 kb on - strand, within GFF29 at 41.244 kb on + strand, within GFF29 at 41.244 kb on + strand, within GFF29 at 41.244 kb on + strand, within GFF29 at 41.245 kb on - strand, within GFF29 at 41.245 kb on - strand, within GFF29 at 41.251 kb on + strand, within GFF29 at 41.252 kb on - strand, within GFF29 at 41.292 kb on + strand, within GFF29 at 41.292 kb on + strand, within GFF29 at 41.292 kb on + strand, within GFF29 at 41.293 kb on - strand, within GFF29 at 41.293 kb on - strand, within GFF29 at 41.293 kb on - strand, within GFF29 at 41.375 kb on + strand, within GFF29 at 41.376 kb on - strand, within GFF29 at 41.386 kb on - strand, within GFF29 at 41.443 kb on + strand, within GFF29 at 41.443 kb on + strand, within GFF29 at 41.443 kb on + strand, within GFF29 at 41.444 kb on - strand, within GFF29 at 41.444 kb on - strand, within GFF29 at 41.444 kb on - strand, within GFF29 at 41.444 kb on - strand, within GFF29 at 41.444 kb on - strand, within GFF29 at 41.444 kb on - strand, within GFF29 at 41.444 kb on - strand, within GFF29 at 41.448 kb on + strand, within GFF29 at 41.449 kb on - strand, within GFF29 at 41.454 kb on + strand, within GFF29 at 41.454 kb on + strand, within GFF29 at 41.478 kb on - strand, within GFF29 at 41.497 kb on + strand, within GFF29 at 41.498 kb on + strand, within GFF29 at 41.498 kb on + strand, within GFF29 at 41.498 kb on + strand, within GFF29 at 41.498 kb on + strand, within GFF29 at 41.498 kb on + strand, within GFF29 at 41.499 kb on - strand, within GFF29 at 41.499 kb on - strand, within GFF29 at 41.499 kb on - strand, within GFF29 at 41.499 kb on - strand, within GFF29 at 41.499 kb on - strand, within GFF29 at 41.500 kb on + strand, within GFF29 at 41.500 kb on + strand, within GFF29 at 41.500 kb on + strand, within GFF29 at 41.501 kb on - strand, within GFF29 at 41.501 kb on - strand, within GFF29 at 41.501 kb on - strand, within GFF29 at 41.590 kb on + strand, within GFF29 at 41.590 kb on + strand, within GFF29 at 41.592 kb on + strand, within GFF29 at 41.592 kb on + strand, within GFF29 at 41.592 kb on + strand, within GFF29 at 41.592 kb on + strand, within GFF29 at 41.592 kb on + strand, within GFF29 at 41.592 kb on + strand, within GFF29 at 41.592 kb on + strand, within GFF29 at 41.592 kb on + strand, within GFF29 at 41.592 kb on + strand, within GFF29 at 41.592 kb on + strand, within GFF29 at 41.593 kb on - strand, within GFF29 at 41.593 kb on - strand, within GFF29 at 41.593 kb on - strand, within GFF29 at 41.593 kb on - strand, within GFF29 at 41.593 kb on - strand, within GFF29 at 41.593 kb on - strand, within GFF29 at 41.593 kb on - strand, within GFF29 at 41.593 kb on - strand, within GFF29 at 41.613 kb on + strand, within GFF29 at 41.613 kb on + strand, within GFF29 at 41.613 kb on + strand, within GFF29 at 41.614 kb on - strand, within GFF29 at 41.614 kb on - strand, within GFF29 at 41.614 kb on - strand, within GFF29 at 41.638 kb on - strand, within GFF29 at 41.675 kb on - strand at 41.725 kb on + strand at 41.725 kb on + strand at 41.726 kb on - strand at 41.726 kb on - strand at 41.726 kb on - strand at 41.769 kb on + strand at 41.787 kb on + strand at 41.811 kb on + strand at 42.125 kb on + strand, within GFF30 at 42.125 kb on + strand, within GFF30 at 42.125 kb on + strand, within GFF30 at 42.126 kb on - strand, within GFF30 at 42.126 kb on - strand, within GFF30 at 42.316 kb on + strand, within GFF30 at 42.317 kb on - strand, within GFF30 at 42.317 kb on - strand, within GFF30 at 42.322 kb on + strand, within GFF30 at 42.346 kb on + strand, within GFF30 at 42.346 kb on + strand, within GFF30 at 42.401 kb on + strand, within GFF30 at 42.450 kb on - strand at 42.484 kb on + strand at 42.500 kb on + strand at 42.508 kb on + strand at 42.508 kb on + strand at 42.547 kb on - strand at 42.604 kb on + strand at 42.677 kb on + strand, within GFF31 at 42.677 kb on + strand, within GFF31 at 42.678 kb on - strand, within GFF31 at 42.716 kb on + strand, within GFF31 at 42.721 kb on + strand, within GFF31 at 42.781 kb on - strand, within GFF31 at 43.028 kb on + strand, within GFF31 at 43.028 kb on + strand, within GFF31 at 43.028 kb on + strand, within GFF31 at 43.028 kb on + strand, within GFF31 at 43.028 kb on + strand, within GFF31 at 43.029 kb on - strand, within GFF31 at 43.029 kb on - strand, within GFF31 at 43.041 kb on + strand, within GFF31 at 43.041 kb on + strand, within GFF31 at 43.042 kb on - strand, within GFF31 at 43.042 kb on - strand, within GFF31 at 43.045 kb on + strand, within GFF31 at 43.045 kb on + strand, within GFF31 at 43.050 kb on + strand, within GFF31 at 43.050 kb on + strand, within GFF31 at 43.050 kb on + strand, within GFF31 at 43.051 kb on - strand, within GFF31 at 43.053 kb on + strand, within GFF31 at 43.053 kb on + strand, within GFF31 at 43.054 kb on - strand, within GFF31 at 43.104 kb on + strand, within GFF31 at 43.104 kb on + strand, within GFF31 at 43.104 kb on + strand, within GFF31 at 43.104 kb on + strand, within GFF31 at 43.104 kb on + strand, within GFF31 at 43.104 kb on + strand, within GFF31 at 43.104 kb on + strand, within GFF31 at 43.104 kb on + strand, within GFF31 at 43.104 kb on + strand, within GFF31 at 43.104 kb on + strand, within GFF31 at 43.104 kb on + strand, within GFF31 at 43.105 kb on - strand, within GFF31 at 43.105 kb on - strand, within GFF31 at 43.105 kb on - strand, within GFF31 at 43.105 kb on - strand, within GFF31 at 43.105 kb on - strand, within GFF31 at 43.105 kb on - strand, within GFF31 at 43.106 kb on + strand, within GFF31 at 43.106 kb on + strand, within GFF31 at 43.106 kb on + strand, within GFF31 at 43.106 kb on + strand, within GFF31 at 43.106 kb on + strand, within GFF31 at 43.106 kb on + strand, within GFF31 at 43.107 kb on - strand, within GFF31 at 43.116 kb on + strand, within GFF31 at 43.116 kb on + strand, within GFF31 at 43.116 kb on + strand, within GFF31 at 43.116 kb on + strand, within GFF31 at 43.118 kb on + strand, within GFF31 at 43.118 kb on + strand, within GFF31 at 43.118 kb on + strand, within GFF31 at 43.118 kb on + strand, within GFF31 at 43.119 kb on - strand, within GFF31 at 43.191 kb on + strand at 43.215 kb on - strand at 43.215 kb on - strand at 43.233 kb on + strand at 43.233 kb on + strand at 43.234 kb on - strand at 43.278 kb on - strand at 43.323 kb on + strand at 43.330 kb on + strand at 43.331 kb on - strand at 43.331 kb on - strand at 43.333 kb on + strand at 43.385 kb on + strand at 43.419 kb on + strand at 43.443 kb on + strand, within GFF32 at 43.465 kb on - strand, within GFF32
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 40,860 + GFF29 0.42 -1.1 40,894 - GFF29 0.44 -0.4 40,901 - GFF29 0.45 -0.4 40,901 - GFF29 0.45 +0.6 40,917 - GFF29 0.46 -0.6 40,947 + GFF29 0.47 -0.6 41,028 + GFF29 0.52 +0.0 41,028 + GFF29 0.52 -0.8 41,029 - GFF29 0.52 +0.0 41,034 + GFF29 0.53 -1.3 41,035 - GFF29 0.53 -0.8 41,100 + GFF29 0.57 -0.3 41,118 + GFF29 0.58 -1.1 41,119 - GFF29 0.58 +0.6 41,126 + GFF29 0.58 +0.0 41,126 + GFF29 0.58 -1.3 41,126 + GFF29 0.58 -0.4 41,127 - GFF29 0.58 -0.6 41,127 - GFF29 0.58 -0.8 41,127 - GFF29 0.58 -2.1 41,127 - GFF29 0.58 -0.0 41,127 - GFF29 0.58 +0.4 41,128 + GFF29 0.58 -1.2 41,128 + GFF29 0.58 -2.3 41,128 + GFF29 0.58 +0.4 41,128 + GFF29 0.58 -0.3 41,128 + GFF29 0.58 +0.0 41,128 + GFF29 0.58 -1.2 41,129 - GFF29 0.59 -2.1 41,129 - GFF29 0.59 -0.4 41,129 - GFF29 0.59 -0.9 41,129 - GFF29 0.59 +0.4 41,129 - GFF29 0.59 +0.6 41,164 - GFF29 0.61 -0.6 41,172 + GFF29 0.61 -1.2 41,173 - GFF29 0.61 +0.6 41,173 - GFF29 0.61 -0.4 41,194 + GFF29 0.63 -1.0 41,194 + GFF29 0.63 -1.9 41,195 - GFF29 0.63 -0.3 41,195 - GFF29 0.63 -0.1 41,197 + GFF29 0.63 -0.0 41,197 + GFF29 0.63 -0.2 41,198 - GFF29 0.63 -0.3 41,198 - GFF29 0.63 -0.4 41,198 - GFF29 0.63 -1.0 41,198 - GFF29 0.63 -1.0 41,220 + GFF29 0.64 +0.0 41,221 - GFF29 0.64 -1.0 41,244 + GFF29 0.66 +1.0 41,244 + GFF29 0.66 +1.0 41,244 + GFF29 0.66 -0.1 41,245 - GFF29 0.66 +0.4 41,245 - GFF29 0.66 +0.0 41,251 + GFF29 0.66 +1.0 41,252 - GFF29 0.66 -0.8 41,292 + GFF29 0.69 +0.6 41,292 + GFF29 0.69 -0.1 41,292 + GFF29 0.69 -0.6 41,293 - GFF29 0.69 -0.7 41,293 - GFF29 0.69 -1.5 41,293 - GFF29 0.69 -1.6 41,375 + GFF29 0.74 +0.9 41,376 - GFF29 0.74 -0.6 41,386 - GFF29 0.74 -1.0 41,443 + GFF29 0.78 +0.2 41,443 + GFF29 0.78 -0.3 41,443 + GFF29 0.78 -0.8 41,444 - GFF29 0.78 -1.1 41,444 - GFF29 0.78 -0.8 41,444 - GFF29 0.78 +0.4 41,444 - GFF29 0.78 -0.9 41,444 - GFF29 0.78 -0.3 41,444 - GFF29 0.78 +0.0 41,444 - GFF29 0.78 -3.1 41,448 + GFF29 0.78 +0.6 41,449 - GFF29 0.78 +0.0 41,454 + GFF29 0.79 -0.5 41,454 + GFF29 0.79 +0.0 41,478 - GFF29 0.80 -0.7 41,497 + GFF29 0.81 -0.3 41,498 + GFF29 0.81 +0.9 41,498 + GFF29 0.81 -0.1 41,498 + GFF29 0.81 -2.1 41,498 + GFF29 0.81 -0.7 41,498 + GFF29 0.81 -0.3 41,499 - GFF29 0.81 +0.4 41,499 - GFF29 0.81 -2.0 41,499 - GFF29 0.81 -1.0 41,499 - GFF29 0.81 +0.6 41,499 - GFF29 0.81 +0.6 41,500 + GFF29 0.81 -1.0 41,500 + GFF29 0.81 -0.8 41,500 + GFF29 0.81 +0.2 41,501 - GFF29 0.81 -0.3 41,501 - GFF29 0.81 -2.1 41,501 - GFF29 0.81 +0.7 41,590 + GFF29 0.87 -0.4 41,590 + GFF29 0.87 -1.0 41,592 + GFF29 0.87 -0.1 41,592 + GFF29 0.87 +0.3 41,592 + GFF29 0.87 -1.3 41,592 + GFF29 0.87 -0.4 41,592 + GFF29 0.87 -0.3 41,592 + GFF29 0.87 -0.7 41,592 + GFF29 0.87 +0.8 41,592 + GFF29 0.87 +0.4 41,592 + GFF29 0.87 -0.3 41,592 + GFF29 0.87 -1.6 41,593 - GFF29 0.87 +0.3 41,593 - GFF29 0.87 -1.6 41,593 - GFF29 0.87 -0.3 41,593 - GFF29 0.87 -1.0 41,593 - GFF29 0.87 -0.4 41,593 - GFF29 0.87 +0.0 41,593 - GFF29 0.87 +0.0 41,593 - GFF29 0.87 -1.8 41,613 + GFF29 0.88 +0.0 41,613 + GFF29 0.88 -0.4 41,613 + GFF29 0.88 +0.8 41,614 - GFF29 0.88 +0.3 41,614 - GFF29 0.88 -0.6 41,614 - GFF29 0.88 -1.1 41,638 - GFF29 0.90 -0.6 41,675 - -1.1 41,725 + -1.0 41,725 + -1.0 41,726 - -1.3 41,726 - +1.3 41,726 - -1.3 41,769 + +0.2 41,787 + -1.6 41,811 + +0.0 42,125 + GFF30 0.46 -2.1 42,125 + GFF30 0.46 -0.7 42,125 + GFF30 0.46 -1.2 42,126 - GFF30 0.47 -0.4 42,126 - GFF30 0.47 -2.1 42,316 + GFF30 0.75 -1.6 42,317 - GFF30 0.75 -0.1 42,317 - GFF30 0.75 -0.4 42,322 + GFF30 0.76 -0.1 42,346 + GFF30 0.79 -0.8 42,346 + GFF30 0.79 +0.0 42,401 + GFF30 0.87 -1.0 42,450 - -2.1 42,484 + -0.9 42,500 + -0.4 42,508 + -1.0 42,508 + +0.3 42,547 - -0.1 42,604 + +0.5 42,677 + GFF31 0.21 -1.8 42,677 + GFF31 0.21 -0.4 42,678 - GFF31 0.21 -0.8 42,716 + GFF31 0.26 +0.2 42,721 + GFF31 0.27 +0.5 42,781 - GFF31 0.36 -0.5 43,028 + GFF31 0.72 -0.3 43,028 + GFF31 0.72 -1.6 43,028 + GFF31 0.72 -1.0 43,028 + GFF31 0.72 -0.6 43,028 + GFF31 0.72 +0.9 43,029 - GFF31 0.72 +0.4 43,029 - GFF31 0.72 -0.4 43,041 + GFF31 0.74 -0.4 43,041 + GFF31 0.74 +0.2 43,042 - GFF31 0.74 -1.3 43,042 - GFF31 0.74 -0.7 43,045 + GFF31 0.74 -1.0 43,045 + GFF31 0.74 -0.1 43,050 + GFF31 0.75 -2.0 43,050 + GFF31 0.75 -1.6 43,050 + GFF31 0.75 +0.0 43,051 - GFF31 0.75 +0.4 43,053 + GFF31 0.75 -0.4 43,053 + GFF31 0.75 -0.1 43,054 - GFF31 0.75 -0.7 43,104 + GFF31 0.83 -1.0 43,104 + GFF31 0.83 -0.8 43,104 + GFF31 0.83 +0.6 43,104 + GFF31 0.83 +0.0 43,104 + GFF31 0.83 -1.0 43,104 + GFF31 0.83 +0.0 43,104 + GFF31 0.83 -0.4 43,104 + GFF31 0.83 -1.8 43,104 + GFF31 0.83 +0.4 43,104 + GFF31 0.83 -1.1 43,104 + GFF31 0.83 -1.6 43,105 - GFF31 0.83 -0.5 43,105 - GFF31 0.83 +0.6 43,105 - GFF31 0.83 -0.3 43,105 - GFF31 0.83 +1.3 43,105 - GFF31 0.83 +0.0 43,105 - GFF31 0.83 -0.3 43,106 + GFF31 0.83 -0.6 43,106 + GFF31 0.83 -0.4 43,106 + GFF31 0.83 +0.1 43,106 + GFF31 0.83 -0.5 43,106 + GFF31 0.83 -1.6 43,106 + GFF31 0.83 -1.0 43,107 - GFF31 0.83 -1.3 43,116 + GFF31 0.84 -1.3 43,116 + GFF31 0.84 -0.6 43,116 + GFF31 0.84 -0.4 43,116 + GFF31 0.84 +0.3 43,118 + GFF31 0.85 -0.8 43,118 + GFF31 0.85 -0.3 43,118 + GFF31 0.85 -0.7 43,118 + GFF31 0.85 +0.0 43,119 - GFF31 0.85 -1.3 43,191 + +0.1 43,215 - -0.9 43,215 - +0.0 43,233 + -0.3 43,233 + -0.4 43,234 - +0.6 43,278 - -0.3 43,323 + -0.4 43,330 + -0.2 43,331 - -0.6 43,331 - -0.7 43,333 + -1.3 43,385 + -0.3 43,419 + +0.0 43,443 + GFF32 0.10 -2.3 43,465 - GFF32 0.13 -1.0
Or see this region's nucleotide sequence