Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF280 and GFF281 are separated by 116 nucleotides GFF281 and GFF282 are separated by 6 nucleotides GFF282 and GFF283 overlap by 4 nucleotides
GFF280 - Lipid A biosynthesis lauroyltransferase, at 297,337 to 298,212
GFF280
GFF281 - Protein InaA, at 298,329 to 299,114
GFF281
GFF282 - hypothetical protein, at 299,121 to 300,086
GFF282
GFF283 - hypothetical protein, at 300,083 to 301,531
GFF283
Position (kb) 298
299
300 Strain fitness (log2 ratio) -2
-1
0
1 at 297.338 kb on + strand at 297.367 kb on - strand at 297.390 kb on - strand at 298.283 kb on + strand at 298.283 kb on + strand at 298.304 kb on + strand at 298.313 kb on + strand at 298.313 kb on + strand at 298.314 kb on - strand at 298.314 kb on - strand at 298.324 kb on + strand at 298.521 kb on + strand, within GFF281 at 298.618 kb on + strand, within GFF281 at 298.754 kb on + strand, within GFF281 at 298.822 kb on + strand, within GFF281 at 298.837 kb on + strand, within GFF281 at 298.931 kb on + strand, within GFF281 at 298.931 kb on + strand, within GFF281 at 298.931 kb on + strand, within GFF281 at 298.931 kb on + strand, within GFF281 at 298.931 kb on + strand, within GFF281 at 298.932 kb on - strand, within GFF281 at 299.013 kb on + strand, within GFF281 at 299.031 kb on + strand, within GFF281 at 299.102 kb on - strand at 299.112 kb on + strand at 299.112 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 297,338 + -1.1 297,367 - -1.3 297,390 - +0.1 298,283 + +1.5 298,283 + +0.5 298,304 + -1.1 298,313 + -1.3 298,313 + -0.6 298,314 - -1.3 298,314 - -1.5 298,324 + +0.5 298,521 + GFF281 0.24 +0.1 298,618 + GFF281 0.37 +0.6 298,754 + GFF281 0.54 -0.9 298,822 + GFF281 0.63 -1.5 298,837 + GFF281 0.65 -1.3 298,931 + GFF281 0.77 -1.3 298,931 + GFF281 0.77 -2.0 298,931 + GFF281 0.77 -2.4 298,931 + GFF281 0.77 -1.3 298,931 + GFF281 0.77 -1.3 298,932 - GFF281 0.77 -0.9 299,013 + GFF281 0.87 -0.7 299,031 + GFF281 0.89 +1.1 299,102 - -0.9 299,112 + -1.8 299,112 + -1.2
Or see this region's nucleotide sequence