Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2125 and GFF2126 are separated by 15 nucleotides GFF2126 and GFF2127 are separated by 6 nucleotides GFF2127 and GFF2128 are separated by 44 nucleotides GFF2128 and GFF2129 overlap by 4 nucleotides
GFF2125 - ATP-dependent DNA helicase RecG, at 171,032 to 173,095
GFF2125
GFF2126 - Bifunctional (p)ppGpp synthase/hydrolase SpoT, at 173,111 to 175,327
GFF2126
GFF2127 - DNA-directed RNA polymerase subunit omega, at 175,334 to 175,549
GFF2127
GFF2128 - Guanylate kinase, at 175,594 to 176,208
GFF2128
GFF2129 - hypothetical protein, at 176,205 to 176,939
GFF2129
Position (kb) 173
174
175
176 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 172.111 kb on + strand, within GFF2125 at 172.125 kb on + strand, within GFF2125 at 172.125 kb on + strand, within GFF2125 at 172.126 kb on - strand, within GFF2125 at 172.194 kb on - strand, within GFF2125 at 172.407 kb on + strand, within GFF2125 at 172.425 kb on - strand, within GFF2125 at 172.449 kb on + strand, within GFF2125 at 172.449 kb on + strand, within GFF2125 at 172.449 kb on + strand, within GFF2125 at 172.450 kb on - strand, within GFF2125 at 172.569 kb on + strand, within GFF2125 at 172.569 kb on + strand, within GFF2125 at 172.569 kb on + strand, within GFF2125 at 172.569 kb on + strand, within GFF2125 at 172.570 kb on - strand, within GFF2125 at 172.570 kb on - strand, within GFF2125 at 172.570 kb on - strand, within GFF2125 at 172.570 kb on - strand, within GFF2125 at 172.570 kb on - strand, within GFF2125 at 172.570 kb on - strand, within GFF2125 at 172.570 kb on - strand, within GFF2125 at 172.570 kb on - strand, within GFF2125 at 172.570 kb on - strand, within GFF2125 at 172.570 kb on - strand, within GFF2125 at 172.571 kb on + strand, within GFF2125 at 172.572 kb on - strand, within GFF2125 at 172.572 kb on - strand, within GFF2125 at 172.572 kb on - strand, within GFF2125 at 172.572 kb on - strand, within GFF2125 at 172.640 kb on - strand, within GFF2125 at 172.669 kb on - strand, within GFF2125 at 172.741 kb on - strand, within GFF2125 at 172.778 kb on + strand, within GFF2125 at 172.827 kb on - strand, within GFF2125 at 172.893 kb on + strand at 172.893 kb on + strand at 173.009 kb on + strand at 173.010 kb on - strand at 173.027 kb on - strand at 173.027 kb on - strand at 173.112 kb on + strand at 173.231 kb on - strand at 173.231 kb on - strand at 173.586 kb on - strand, within GFF2126 at 173.599 kb on + strand, within GFF2126 at 173.783 kb on - strand, within GFF2126 at 173.783 kb on - strand, within GFF2126 at 173.789 kb on - strand, within GFF2126 at 174.087 kb on + strand, within GFF2126 at 174.088 kb on - strand, within GFF2126 at 174.088 kb on - strand, within GFF2126 at 174.088 kb on - strand, within GFF2126 at 175.560 kb on - strand at 175.596 kb on - strand at 175.631 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 172,111 + GFF2125 0.52 -1.6 172,125 + GFF2125 0.53 -0.5 172,125 + GFF2125 0.53 -1.1 172,126 - GFF2125 0.53 -1.2 172,194 - GFF2125 0.56 +0.3 172,407 + GFF2125 0.67 -0.5 172,425 - GFF2125 0.67 +0.4 172,449 + GFF2125 0.69 +0.9 172,449 + GFF2125 0.69 -1.5 172,449 + GFF2125 0.69 -0.1 172,450 - GFF2125 0.69 -0.3 172,569 + GFF2125 0.74 -1.1 172,569 + GFF2125 0.74 -0.1 172,569 + GFF2125 0.74 -2.0 172,569 + GFF2125 0.74 -0.3 172,570 - GFF2125 0.75 -0.5 172,570 - GFF2125 0.75 -2.3 172,570 - GFF2125 0.75 -0.1 172,570 - GFF2125 0.75 -0.6 172,570 - GFF2125 0.75 -1.0 172,570 - GFF2125 0.75 -0.1 172,570 - GFF2125 0.75 -0.6 172,570 - GFF2125 0.75 -1.6 172,570 - GFF2125 0.75 -0.1 172,570 - GFF2125 0.75 -0.4 172,571 + GFF2125 0.75 -1.0 172,572 - GFF2125 0.75 +0.9 172,572 - GFF2125 0.75 -1.1 172,572 - GFF2125 0.75 -1.4 172,572 - GFF2125 0.75 -0.6 172,640 - GFF2125 0.78 -2.1 172,669 - GFF2125 0.79 -0.4 172,741 - GFF2125 0.83 -1.1 172,778 + GFF2125 0.85 -2.0 172,827 - GFF2125 0.87 -0.7 172,893 + +0.3 172,893 + -1.7 173,009 + +0.2 173,010 - -0.2 173,027 - -0.6 173,027 - -0.6 173,112 + -2.7 173,231 - -2.1 173,231 - -3.1 173,586 - GFF2126 0.21 -0.7 173,599 + GFF2126 0.22 -1.1 173,783 - GFF2126 0.30 -1.1 173,783 - GFF2126 0.30 -1.0 173,789 - GFF2126 0.31 -2.3 174,087 + GFF2126 0.44 -0.7 174,088 - GFF2126 0.44 -1.1 174,088 - GFF2126 0.44 -0.7 174,088 - GFF2126 0.44 -0.6 175,560 - -1.1 175,596 - +0.0 175,631 - -1.0
Or see this region's nucleotide sequence