Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2324 and GFF2325 are separated by 59 nucleotides GFF2325 and GFF2326 overlap by 4 nucleotides GFF2326 and GFF2327 are separated by 129 nucleotides
GFF2324 - Transcriptional regulatory protein PmpR, at 40,071 to 40,799
GFF2324
GFF2325 - Crossover junction endodeoxyribonuclease RuvC, at 40,859 to 41,419
GFF2325
GFF2326 - Holliday junction ATP-dependent DNA helicase RuvA, at 41,416 to 41,991
GFF2326
GFF2327 - Holliday junction ATP-dependent DNA helicase RuvB, at 42,121 to 43,167
GFF2327
Position (kb) 41
42 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 40.466 kb on + strand, within GFF2324 at 40.466 kb on + strand, within GFF2324 at 40.582 kb on + strand, within GFF2324 at 40.582 kb on + strand, within GFF2324 at 40.582 kb on + strand, within GFF2324 at 40.582 kb on + strand, within GFF2324 at 40.582 kb on + strand, within GFF2324 at 40.582 kb on + strand, within GFF2324 at 40.770 kb on + strand at 40.797 kb on + strand at 40.797 kb on + strand at 40.798 kb on - strand at 40.798 kb on - strand at 40.864 kb on + strand at 40.884 kb on + strand at 40.920 kb on + strand, within GFF2325 at 40.920 kb on + strand, within GFF2325 at 40.920 kb on + strand, within GFF2325 at 40.946 kb on + strand, within GFF2325 at 40.946 kb on + strand, within GFF2325 at 40.947 kb on - strand, within GFF2325 at 41.072 kb on + strand, within GFF2325 at 41.073 kb on - strand, within GFF2325 at 41.111 kb on - strand, within GFF2325 at 41.202 kb on - strand, within GFF2325 at 41.287 kb on + strand, within GFF2325 at 41.287 kb on + strand, within GFF2325 at 41.367 kb on + strand at 41.367 kb on + strand at 41.398 kb on + strand at 41.398 kb on + strand at 41.398 kb on + strand at 41.399 kb on - strand at 41.415 kb on + strand at 41.415 kb on + strand at 41.416 kb on - strand at 41.492 kb on - strand, within GFF2326 at 41.492 kb on - strand, within GFF2326 at 41.498 kb on + strand, within GFF2326 at 41.565 kb on + strand, within GFF2326 at 41.565 kb on + strand, within GFF2326 at 41.566 kb on - strand, within GFF2326 at 41.566 kb on - strand, within GFF2326 at 41.596 kb on + strand, within GFF2326 at 41.596 kb on + strand, within GFF2326 at 41.596 kb on + strand, within GFF2326 at 41.752 kb on - strand, within GFF2326 at 41.866 kb on - strand, within GFF2326 at 41.977 kb on + strand at 42.017 kb on + strand at 42.017 kb on + strand at 42.018 kb on - strand at 42.018 kb on - strand at 42.020 kb on + strand at 42.020 kb on + strand at 42.021 kb on - strand at 42.021 kb on - strand at 42.026 kb on + strand at 42.026 kb on + strand at 42.026 kb on + strand at 42.027 kb on - strand at 42.027 kb on - strand at 42.027 kb on - strand at 42.027 kb on - strand at 42.027 kb on - strand at 42.027 kb on - strand at 42.027 kb on - strand at 42.038 kb on + strand at 42.038 kb on + strand at 42.038 kb on + strand at 42.038 kb on + strand at 42.039 kb on - strand at 42.052 kb on + strand at 42.091 kb on + strand at 42.091 kb on + strand at 42.214 kb on + strand at 42.214 kb on + strand at 42.215 kb on - strand at 42.215 kb on - strand at 42.456 kb on + strand, within GFF2327 at 42.926 kb on + strand, within GFF2327 at 42.926 kb on + strand, within GFF2327
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 40,466 + GFF2324 0.54 -0.7 40,466 + GFF2324 0.54 +0.5 40,582 + GFF2324 0.70 -1.1 40,582 + GFF2324 0.70 -0.7 40,582 + GFF2324 0.70 -1.4 40,582 + GFF2324 0.70 -1.4 40,582 + GFF2324 0.70 -2.1 40,582 + GFF2324 0.70 +0.5 40,770 + -0.3 40,797 + -1.2 40,797 + -1.7 40,798 - -0.9 40,798 - -3.0 40,864 + -1.1 40,884 + +0.5 40,920 + GFF2325 0.11 -1.1 40,920 + GFF2325 0.11 -1.1 40,920 + GFF2325 0.11 -1.1 40,946 + GFF2325 0.16 -1.0 40,946 + GFF2325 0.16 -0.2 40,947 - GFF2325 0.16 -0.1 41,072 + GFF2325 0.38 -2.7 41,073 - GFF2325 0.38 -1.1 41,111 - GFF2325 0.45 -0.1 41,202 - GFF2325 0.61 -2.4 41,287 + GFF2325 0.76 -0.4 41,287 + GFF2325 0.76 -2.9 41,367 + -0.5 41,367 + -0.8 41,398 + -0.4 41,398 + +0.4 41,398 + -0.9 41,399 - -1.4 41,415 + -1.4 41,415 + -1.7 41,416 - -1.1 41,492 - GFF2326 0.13 -1.4 41,492 - GFF2326 0.13 +0.5 41,498 + GFF2326 0.14 -3.2 41,565 + GFF2326 0.26 -1.1 41,565 + GFF2326 0.26 -1.7 41,566 - GFF2326 0.26 -1.4 41,566 - GFF2326 0.26 +0.5 41,596 + GFF2326 0.31 -0.7 41,596 + GFF2326 0.31 -1.7 41,596 + GFF2326 0.31 +1.5 41,752 - GFF2326 0.58 -0.2 41,866 - GFF2326 0.78 -1.4 41,977 + -1.1 42,017 + -0.9 42,017 + +0.2 42,018 - -0.1 42,018 - +0.9 42,020 + -1.0 42,020 + +0.7 42,021 - -1.1 42,021 - -0.8 42,026 + -1.1 42,026 + -0.2 42,026 + -1.0 42,027 - +0.5 42,027 - -0.1 42,027 - -0.5 42,027 - -1.4 42,027 - -0.3 42,027 - +0.2 42,027 - -0.1 42,038 + -1.1 42,038 + -1.4 42,038 + +0.7 42,038 + -1.7 42,039 - +0.9 42,052 + -1.7 42,091 + -0.7 42,091 + +0.2 42,214 + -1.9 42,214 + -0.7 42,215 - -1.4 42,215 - -1.1 42,456 + GFF2327 0.32 -2.5 42,926 + GFF2327 0.77 -0.4 42,926 + GFF2327 0.77 -1.6
Or see this region's nucleotide sequence