Experiment: Methanol and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF155 and GFF156 are separated by 24 nucleotides GFF156 and GFF157 are separated by 13 nucleotides GFF157 and GFF158 are separated by 25 nucleotides GFF158 and GFF159 overlap by 8 nucleotides
GFF155 - FeMo cofactor biosynthesis protein NifB, at 154,893 to 156,452
GFF155
GFF156 - Ferredoxin-1, at 156,477 to 156,695
GFF156
GFF157 - Protein HesB, vegetative, at 156,709 to 157,101
GFF157
GFF158 - hypothetical protein, at 157,127 to 157,648
GFF158
GFF159 - hypothetical protein, at 157,641 to 158,501
GFF159
Position (kb)
156
157 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 155.477 kb on - strand, within GFF155 at 155.536 kb on - strand, within GFF155 at 155.536 kb on - strand, within GFF155 at 155.661 kb on + strand, within GFF155 at 155.661 kb on + strand, within GFF155 at 155.705 kb on + strand, within GFF155 at 155.745 kb on + strand, within GFF155 at 155.931 kb on + strand, within GFF155 at 156.040 kb on + strand, within GFF155 at 156.040 kb on + strand, within GFF155 at 156.120 kb on + strand, within GFF155 at 156.208 kb on + strand, within GFF155 at 156.208 kb on + strand, within GFF155 at 156.209 kb on - strand, within GFF155 at 156.209 kb on - strand, within GFF155 at 156.209 kb on - strand, within GFF155 at 156.215 kb on + strand, within GFF155 at 156.215 kb on + strand, within GFF155 at 156.216 kb on - strand, within GFF155 at 156.216 kb on - strand, within GFF155 at 156.216 kb on - strand, within GFF155 at 156.301 kb on + strand at 156.319 kb on - strand at 156.319 kb on - strand at 156.401 kb on + strand at 156.441 kb on - strand at 156.457 kb on + strand at 156.458 kb on - strand at 156.462 kb on + strand at 156.463 kb on - strand at 156.463 kb on - strand at 156.483 kb on + strand at 156.484 kb on - strand at 156.594 kb on - strand, within GFF156 at 156.646 kb on + strand, within GFF156 at 156.646 kb on + strand, within GFF156 at 156.662 kb on + strand, within GFF156 at 156.663 kb on - strand, within GFF156 at 156.667 kb on - strand, within GFF156 at 156.668 kb on + strand, within GFF156 at 156.669 kb on - strand, within GFF156 at 156.676 kb on - strand at 156.693 kb on + strand at 156.744 kb on + strand at 156.800 kb on + strand, within GFF157 at 156.801 kb on - strand, within GFF157 at 156.801 kb on - strand, within GFF157 at 156.801 kb on - strand, within GFF157 at 156.801 kb on - strand, within GFF157 at 156.893 kb on + strand, within GFF157 at 156.894 kb on - strand, within GFF157 at 156.927 kb on - strand, within GFF157 at 156.927 kb on - strand, within GFF157 at 156.951 kb on + strand, within GFF157 at 156.952 kb on - strand, within GFF157 at 156.952 kb on - strand, within GFF157 at 156.994 kb on - strand, within GFF157 at 157.087 kb on - strand at 157.181 kb on + strand, within GFF158 at 157.182 kb on - strand, within GFF158 at 157.228 kb on + strand, within GFF158 at 157.235 kb on + strand, within GFF158 at 157.236 kb on - strand, within GFF158 at 157.284 kb on + strand, within GFF158 at 157.328 kb on + strand, within GFF158 at 157.379 kb on - strand, within GFF158 at 157.482 kb on + strand, within GFF158 at 157.483 kb on - strand, within GFF158 at 157.499 kb on + strand, within GFF158 at 157.500 kb on - strand, within GFF158 at 157.500 kb on - strand, within GFF158 at 157.500 kb on - strand, within GFF158 at 157.500 kb on - strand, within GFF158 at 157.602 kb on + strand at 157.603 kb on - strand at 157.658 kb on + strand at 157.658 kb on + strand at 157.658 kb on + strand at 157.658 kb on + strand at 157.659 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol and casaminos (C) remove 155,477 - GFF155 0.37 -1.0 155,536 - GFF155 0.41 -0.8 155,536 - GFF155 0.41 +0.0 155,661 + GFF155 0.49 -0.3 155,661 + GFF155 0.49 +0.0 155,705 + GFF155 0.52 -2.2 155,745 + GFF155 0.55 +0.0 155,931 + GFF155 0.67 +0.2 156,040 + GFF155 0.74 -0.2 156,040 + GFF155 0.74 -0.8 156,120 + GFF155 0.79 +0.3 156,208 + GFF155 0.84 -1.3 156,208 + GFF155 0.84 -1.3 156,209 - GFF155 0.84 -1.0 156,209 - GFF155 0.84 -1.0 156,209 - GFF155 0.84 -0.6 156,215 + GFF155 0.85 -0.1 156,215 + GFF155 0.85 -1.5 156,216 - GFF155 0.85 -1.3 156,216 - GFF155 0.85 -0.5 156,216 - GFF155 0.85 +0.3 156,301 + -0.6 156,319 - -1.4 156,319 - -1.0 156,401 + -1.8 156,441 - +0.4 156,457 + -0.5 156,458 - -1.3 156,462 + -0.8 156,463 - +0.3 156,463 - +0.3 156,483 + +0.3 156,484 - -0.7 156,594 - GFF156 0.53 -0.1 156,646 + GFF156 0.77 -1.0 156,646 + GFF156 0.77 -0.2 156,662 + GFF156 0.84 -1.7 156,663 - GFF156 0.85 -2.0 156,667 - GFF156 0.87 +0.0 156,668 + GFF156 0.87 -0.8 156,669 - GFF156 0.88 -1.6 156,676 - -0.5 156,693 + +0.0 156,744 + -1.2 156,800 + GFF157 0.23 -1.2 156,801 - GFF157 0.23 -1.0 156,801 - GFF157 0.23 -1.0 156,801 - GFF157 0.23 -0.6 156,801 - GFF157 0.23 -0.6 156,893 + GFF157 0.47 +0.4 156,894 - GFF157 0.47 -1.0 156,927 - GFF157 0.55 -0.4 156,927 - GFF157 0.55 +0.5 156,951 + GFF157 0.62 -1.0 156,952 - GFF157 0.62 +0.4 156,952 - GFF157 0.62 -2.3 156,994 - GFF157 0.73 +0.4 157,087 - -0.8 157,181 + GFF158 0.10 -0.3 157,182 - GFF158 0.11 -0.3 157,228 + GFF158 0.19 +0.0 157,235 + GFF158 0.21 -2.6 157,236 - GFF158 0.21 +0.0 157,284 + GFF158 0.30 -1.1 157,328 + GFF158 0.39 -0.2 157,379 - GFF158 0.48 -2.2 157,482 + GFF158 0.68 -3.2 157,483 - GFF158 0.68 -1.0 157,499 + GFF158 0.71 +0.7 157,500 - GFF158 0.71 -1.6 157,500 - GFF158 0.71 -0.3 157,500 - GFF158 0.71 -0.6 157,500 - GFF158 0.71 -0.4 157,602 + -1.6 157,603 - -0.8 157,658 + -0.8 157,658 + -1.3 157,658 + +0.7 157,658 + -0.4 157,659 - -1.3
Or see this region's nucleotide sequence