Experiment: RPMI_TSB_serum temperature; 30C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR41_RS18655 and RR41_RS18660 are separated by 192 nucleotides RR41_RS18660 and RR41_RS18665 are separated by 48 nucleotides RR41_RS18665 and RR41_RS18670 are separated by 44 nucleotides
RR41_RS18655: RR41_RS18655 - polysaccharide biosynthesis tyrosine autokinase, at 3,880,493 to 3,882,676
_RS18655
RR41_RS18660: RR41_RS18660 - FKBP-type peptidyl-prolyl cis-trans isomerase, at 3,882,869 to 3,883,592
_RS18660
RR41_RS18665: RR41_RS18665 - FKBP-type peptidyl-prolyl cis-trans isomerase, at 3,883,641 to 3,884,336
_RS18665
RR41_RS18670: RR41_RS18670 - murein biosynthesis integral membrane protein MurJ, at 3,884,381 to 3,885,931
_RS18670
Position (kb)
3883
3884
3885 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3882.641 kb on + strand at 3882.641 kb on + strand at 3882.641 kb on + strand at 3882.641 kb on + strand at 3882.641 kb on + strand at 3882.641 kb on + strand at 3882.641 kb on + strand at 3882.642 kb on - strand at 3882.642 kb on - strand at 3882.642 kb on - strand at 3882.642 kb on - strand at 3882.647 kb on + strand at 3882.647 kb on + strand at 3882.647 kb on + strand at 3882.648 kb on - strand at 3882.648 kb on - strand at 3882.674 kb on + strand at 3882.675 kb on - strand at 3882.678 kb on + strand at 3882.678 kb on + strand at 3882.679 kb on - strand at 3882.679 kb on - strand at 3882.762 kb on - strand at 3882.764 kb on - strand at 3882.766 kb on - strand at 3882.799 kb on - strand at 3882.799 kb on - strand at 3882.804 kb on + strand at 3882.804 kb on + strand at 3882.820 kb on - strand at 3882.820 kb on - strand at 3882.820 kb on - strand at 3882.838 kb on + strand at 3882.839 kb on - strand at 3882.839 kb on - strand at 3882.924 kb on + strand at 3882.925 kb on - strand at 3882.925 kb on - strand at 3882.932 kb on + strand at 3882.932 kb on + strand at 3882.935 kb on + strand at 3882.971 kb on - strand, within RR41_RS18660 at 3883.011 kb on - strand, within RR41_RS18660 at 3883.054 kb on - strand, within RR41_RS18660 at 3883.054 kb on - strand, within RR41_RS18660 at 3883.057 kb on + strand, within RR41_RS18660 at 3883.058 kb on - strand, within RR41_RS18660 at 3883.058 kb on - strand, within RR41_RS18660 at 3883.060 kb on + strand, within RR41_RS18660 at 3883.061 kb on - strand, within RR41_RS18660 at 3883.096 kb on + strand, within RR41_RS18660 at 3883.097 kb on - strand, within RR41_RS18660 at 3883.112 kb on + strand, within RR41_RS18660 at 3883.112 kb on + strand, within RR41_RS18660 at 3883.112 kb on + strand, within RR41_RS18660 at 3883.114 kb on + strand, within RR41_RS18660 at 3883.265 kb on - strand, within RR41_RS18660 at 3883.290 kb on + strand, within RR41_RS18660 at 3883.290 kb on + strand, within RR41_RS18660 at 3883.291 kb on - strand, within RR41_RS18660 at 3883.291 kb on - strand, within RR41_RS18660 at 3883.291 kb on - strand, within RR41_RS18660 at 3883.292 kb on + strand, within RR41_RS18660 at 3883.292 kb on + strand, within RR41_RS18660 at 3883.292 kb on + strand, within RR41_RS18660 at 3883.292 kb on + strand, within RR41_RS18660 at 3883.292 kb on + strand, within RR41_RS18660 at 3883.292 kb on + strand, within RR41_RS18660 at 3883.292 kb on + strand, within RR41_RS18660 at 3883.292 kb on + strand, within RR41_RS18660 at 3883.293 kb on - strand, within RR41_RS18660 at 3883.293 kb on - strand, within RR41_RS18660 at 3883.293 kb on - strand, within RR41_RS18660 at 3883.298 kb on + strand, within RR41_RS18660 at 3883.299 kb on - strand, within RR41_RS18660 at 3883.299 kb on - strand, within RR41_RS18660 at 3883.302 kb on - strand, within RR41_RS18660 at 3883.338 kb on - strand, within RR41_RS18660 at 3883.338 kb on - strand, within RR41_RS18660 at 3883.338 kb on - strand, within RR41_RS18660 at 3883.344 kb on - strand, within RR41_RS18660 at 3883.349 kb on - strand, within RR41_RS18660 at 3883.349 kb on - strand, within RR41_RS18660 at 3883.353 kb on + strand, within RR41_RS18660 at 3883.354 kb on - strand, within RR41_RS18660 at 3883.354 kb on - strand, within RR41_RS18660 at 3883.354 kb on - strand, within RR41_RS18660 at 3883.388 kb on + strand, within RR41_RS18660 at 3883.395 kb on + strand, within RR41_RS18660 at 3883.395 kb on + strand, within RR41_RS18660 at 3883.395 kb on + strand, within RR41_RS18660 at 3883.395 kb on + strand, within RR41_RS18660 at 3883.396 kb on - strand, within RR41_RS18660 at 3883.396 kb on - strand, within RR41_RS18660 at 3883.396 kb on - strand, within RR41_RS18660 at 3883.396 kb on - strand, within RR41_RS18660 at 3883.396 kb on - strand, within RR41_RS18660 at 3883.397 kb on + strand, within RR41_RS18660 at 3883.498 kb on + strand, within RR41_RS18660 at 3883.498 kb on + strand, within RR41_RS18660 at 3883.499 kb on - strand, within RR41_RS18660 at 3883.570 kb on + strand at 3883.570 kb on + strand at 3883.570 kb on + strand at 3883.570 kb on + strand at 3883.594 kb on - strand at 3883.614 kb on - strand at 3883.614 kb on - strand at 3883.614 kb on - strand at 3883.615 kb on + strand at 3883.615 kb on + strand at 3883.615 kb on + strand at 3883.615 kb on + strand at 3883.615 kb on + strand at 3883.615 kb on + strand at 3883.615 kb on + strand at 3883.615 kb on + strand at 3883.616 kb on - strand at 3883.684 kb on + strand at 3883.715 kb on + strand, within RR41_RS18665 at 3883.740 kb on - strand, within RR41_RS18665 at 3883.745 kb on + strand, within RR41_RS18665 at 3883.746 kb on - strand, within RR41_RS18665 at 3883.746 kb on - strand, within RR41_RS18665 at 3883.746 kb on - strand, within RR41_RS18665 at 3883.749 kb on + strand, within RR41_RS18665 at 3883.750 kb on - strand, within RR41_RS18665 at 3883.750 kb on - strand, within RR41_RS18665 at 3883.755 kb on + strand, within RR41_RS18665 at 3883.773 kb on - strand, within RR41_RS18665 at 3883.776 kb on - strand, within RR41_RS18665 at 3883.776 kb on - strand, within RR41_RS18665 at 3883.842 kb on - strand, within RR41_RS18665 at 3883.883 kb on + strand, within RR41_RS18665 at 3883.883 kb on + strand, within RR41_RS18665 at 3883.893 kb on - strand, within RR41_RS18665 at 3883.918 kb on + strand, within RR41_RS18665 at 3884.052 kb on - strand, within RR41_RS18665 at 3884.060 kb on + strand, within RR41_RS18665 at 3884.084 kb on - strand, within RR41_RS18665 at 3884.089 kb on - strand, within RR41_RS18665 at 3884.089 kb on - strand, within RR41_RS18665 at 3884.089 kb on - strand, within RR41_RS18665 at 3884.124 kb on - strand, within RR41_RS18665 at 3884.139 kb on - strand, within RR41_RS18665 at 3884.217 kb on - strand, within RR41_RS18665 at 3884.251 kb on - strand, within RR41_RS18665 at 3884.273 kb on + strand at 3884.276 kb on + strand at 3884.276 kb on + strand at 3884.277 kb on - strand at 3884.277 kb on - strand at 3884.277 kb on - strand at 3884.277 kb on - strand at 3884.297 kb on - strand at 3884.317 kb on + strand at 3884.317 kb on + strand at 3884.318 kb on - strand at 3884.318 kb on - strand at 3884.334 kb on + strand at 3884.382 kb on + strand at 3884.383 kb on - strand at 3884.391 kb on - strand at 3884.418 kb on - strand at 3884.418 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RPMI_TSB_serum temperature; 30C remove 3,882,641 + -1.5 3,882,641 + -0.6 3,882,641 + -2.5 3,882,641 + -1.8 3,882,641 + +0.8 3,882,641 + -1.2 3,882,641 + -0.2 3,882,642 - -0.5 3,882,642 - -1.2 3,882,642 - -2.5 3,882,642 - -0.9 3,882,647 + -0.9 3,882,647 + -2.2 3,882,647 + +1.0 3,882,648 - -0.3 3,882,648 - +0.1 3,882,674 + +1.7 3,882,675 - -2.2 3,882,678 + +0.9 3,882,678 + -1.2 3,882,679 - -1.5 3,882,679 - +1.1 3,882,762 - -0.2 3,882,764 - +2.3 3,882,766 - -0.4 3,882,799 - +0.4 3,882,799 - +0.3 3,882,804 + +0.8 3,882,804 + +0.8 3,882,820 - +1.4 3,882,820 - -0.2 3,882,820 - -0.7 3,882,838 + -0.6 3,882,839 - -1.3 3,882,839 - -1.2 3,882,924 + -0.2 3,882,925 - -0.2 3,882,925 - +1.6 3,882,932 + +0.8 3,882,932 + -1.2 3,882,935 + -1.8 3,882,971 - RR41_RS18660 0.14 -1.2 3,883,011 - RR41_RS18660 0.20 -1.2 3,883,054 - RR41_RS18660 0.26 -0.8 3,883,054 - RR41_RS18660 0.26 -2.4 3,883,057 + RR41_RS18660 0.26 -0.8 3,883,058 - RR41_RS18660 0.26 -2.5 3,883,058 - RR41_RS18660 0.26 -1.8 3,883,060 + RR41_RS18660 0.26 -1.8 3,883,061 - RR41_RS18660 0.27 -2.5 3,883,096 + RR41_RS18660 0.31 +0.8 3,883,097 - RR41_RS18660 0.31 +2.1 3,883,112 + RR41_RS18660 0.34 +0.3 3,883,112 + RR41_RS18660 0.34 -0.2 3,883,112 + RR41_RS18660 0.34 -2.2 3,883,114 + RR41_RS18660 0.34 -0.2 3,883,265 - RR41_RS18660 0.55 -0.8 3,883,290 + RR41_RS18660 0.58 +1.6 3,883,290 + RR41_RS18660 0.58 -2.0 3,883,291 - RR41_RS18660 0.58 -2.2 3,883,291 - RR41_RS18660 0.58 +0.0 3,883,291 - RR41_RS18660 0.58 -1.2 3,883,292 + RR41_RS18660 0.58 -1.8 3,883,292 + RR41_RS18660 0.58 -0.7 3,883,292 + RR41_RS18660 0.58 -0.6 3,883,292 + RR41_RS18660 0.58 -0.2 3,883,292 + RR41_RS18660 0.58 -0.7 3,883,292 + RR41_RS18660 0.58 +0.7 3,883,292 + RR41_RS18660 0.58 +1.4 3,883,292 + RR41_RS18660 0.58 -2.5 3,883,293 - RR41_RS18660 0.59 +0.5 3,883,293 - RR41_RS18660 0.59 -2.5 3,883,293 - RR41_RS18660 0.59 -0.6 3,883,298 + RR41_RS18660 0.59 -0.2 3,883,299 - RR41_RS18660 0.59 -2.2 3,883,299 - RR41_RS18660 0.59 +0.8 3,883,302 - RR41_RS18660 0.60 -2.2 3,883,338 - RR41_RS18660 0.65 +0.2 3,883,338 - RR41_RS18660 0.65 -2.2 3,883,338 - RR41_RS18660 0.65 +3.6 3,883,344 - RR41_RS18660 0.66 +0.0 3,883,349 - RR41_RS18660 0.66 -0.8 3,883,349 - RR41_RS18660 0.66 -0.9 3,883,353 + RR41_RS18660 0.67 +1.1 3,883,354 - RR41_RS18660 0.67 -1.5 3,883,354 - RR41_RS18660 0.67 -1.8 3,883,354 - RR41_RS18660 0.67 +0.4 3,883,388 + RR41_RS18660 0.72 -0.5 3,883,395 + RR41_RS18660 0.73 -0.2 3,883,395 + RR41_RS18660 0.73 -2.2 3,883,395 + RR41_RS18660 0.73 -2.2 3,883,395 + RR41_RS18660 0.73 +0.6 3,883,396 - RR41_RS18660 0.73 +0.4 3,883,396 - RR41_RS18660 0.73 -1.8 3,883,396 - RR41_RS18660 0.73 +1.4 3,883,396 - RR41_RS18660 0.73 -1.5 3,883,396 - RR41_RS18660 0.73 +0.1 3,883,397 + RR41_RS18660 0.73 -1.6 3,883,498 + RR41_RS18660 0.87 -1.8 3,883,498 + RR41_RS18660 0.87 -0.1 3,883,499 - RR41_RS18660 0.87 -1.8 3,883,570 + +0.8 3,883,570 + -0.2 3,883,570 + +1.0 3,883,570 + -1.2 3,883,594 - -1.2 3,883,614 - -0.5 3,883,614 - -0.2 3,883,614 - -2.5 3,883,615 + -1.2 3,883,615 + -1.4 3,883,615 + +1.8 3,883,615 + -0.1 3,883,615 + +1.5 3,883,615 + +1.0 3,883,615 + -1.4 3,883,615 + -2.1 3,883,616 - +0.4 3,883,684 + +1.4 3,883,715 + RR41_RS18665 0.11 -1.8 3,883,740 - RR41_RS18665 0.14 -1.8 3,883,745 + RR41_RS18665 0.15 -0.4 3,883,746 - RR41_RS18665 0.15 -0.2 3,883,746 - RR41_RS18665 0.15 -1.8 3,883,746 - RR41_RS18665 0.15 -1.8 3,883,749 + RR41_RS18665 0.16 -2.8 3,883,750 - RR41_RS18665 0.16 -1.0 3,883,750 - RR41_RS18665 0.16 -3.7 3,883,755 + RR41_RS18665 0.16 -1.5 3,883,773 - RR41_RS18665 0.19 -1.2 3,883,776 - RR41_RS18665 0.19 -2.2 3,883,776 - RR41_RS18665 0.19 +0.3 3,883,842 - RR41_RS18665 0.29 -0.2 3,883,883 + RR41_RS18665 0.35 -1.8 3,883,883 + RR41_RS18665 0.35 +0.1 3,883,893 - RR41_RS18665 0.36 -3.0 3,883,918 + RR41_RS18665 0.40 -1.2 3,884,052 - RR41_RS18665 0.59 -1.8 3,884,060 + RR41_RS18665 0.60 -0.2 3,884,084 - RR41_RS18665 0.64 -1.8 3,884,089 - RR41_RS18665 0.64 -0.7 3,884,089 - RR41_RS18665 0.64 -1.4 3,884,089 - RR41_RS18665 0.64 -1.8 3,884,124 - RR41_RS18665 0.69 -0.2 3,884,139 - RR41_RS18665 0.72 +1.4 3,884,217 - RR41_RS18665 0.83 -3.4 3,884,251 - RR41_RS18665 0.88 -2.2 3,884,273 + -1.6 3,884,276 + -1.5 3,884,276 + -0.2 3,884,277 - -0.8 3,884,277 - +1.8 3,884,277 - -0.2 3,884,277 - -0.2 3,884,297 - -0.2 3,884,317 + -1.3 3,884,317 + -0.5 3,884,318 - -0.8 3,884,318 - -3.4 3,884,334 + -1.1 3,884,382 + +0.1 3,884,383 - -1.8 3,884,391 - -0.5 3,884,418 - +1.6 3,884,418 - -1.8
Or see this region's nucleotide sequence