Experiment: RPMI_TSB_serum temperature; 30C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR41_RS16180 and RR41_RS16185 are separated by 124 nucleotides RR41_RS16185 and RR41_RS16190 overlap by 1 nucleotides RR41_RS16190 and RR41_RS16195 are separated by 87 nucleotides RR41_RS16195 and RR41_RS16200 are separated by 69 nucleotides
RR41_RS16180: RR41_RS16180 - aconitate hydratase AcnA, at 3,359,381 to 3,362,137
_RS16180
RR41_RS16185: RR41_RS16185 - TatD family hydrolase, at 3,362,262 to 3,363,074
_RS16185
RR41_RS16190: RR41_RS16190 - RsiV family protein, at 3,363,074 to 3,364,015
_RS16190
RR41_RS16195: RR41_RS16195 - hypothetical protein, at 3,364,103 to 3,364,945
_RS16195
RR41_RS16200: RR41_RS16200 - peptide chain release factor 3, at 3,365,015 to 3,366,604
_RS16200
Position (kb)
3363
3364
3365 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3362.084 kb on + strand at 3362.085 kb on - strand at 3362.085 kb on - strand at 3362.126 kb on - strand at 3362.126 kb on - strand at 3362.127 kb on + strand at 3362.127 kb on + strand at 3362.127 kb on + strand at 3362.238 kb on - strand at 3362.240 kb on + strand at 3362.240 kb on + strand at 3362.240 kb on + strand at 3362.241 kb on - strand at 3362.260 kb on + strand at 3362.328 kb on - strand at 3362.328 kb on - strand at 3362.355 kb on + strand, within RR41_RS16185 at 3362.356 kb on - strand, within RR41_RS16185 at 3362.356 kb on - strand, within RR41_RS16185 at 3362.365 kb on - strand, within RR41_RS16185 at 3362.365 kb on - strand, within RR41_RS16185 at 3362.466 kb on + strand, within RR41_RS16185 at 3362.466 kb on + strand, within RR41_RS16185 at 3362.469 kb on - strand, within RR41_RS16185 at 3362.547 kb on - strand, within RR41_RS16185 at 3362.623 kb on - strand, within RR41_RS16185 at 3362.655 kb on + strand, within RR41_RS16185 at 3362.697 kb on + strand, within RR41_RS16185 at 3362.713 kb on + strand, within RR41_RS16185 at 3362.731 kb on + strand, within RR41_RS16185 at 3362.731 kb on + strand, within RR41_RS16185 at 3362.731 kb on + strand, within RR41_RS16185 at 3362.731 kb on + strand, within RR41_RS16185 at 3362.732 kb on - strand, within RR41_RS16185 at 3362.732 kb on - strand, within RR41_RS16185 at 3362.732 kb on - strand, within RR41_RS16185 at 3362.732 kb on - strand, within RR41_RS16185 at 3362.732 kb on - strand, within RR41_RS16185 at 3362.733 kb on + strand, within RR41_RS16185 at 3362.733 kb on + strand, within RR41_RS16185 at 3362.734 kb on - strand, within RR41_RS16185 at 3362.735 kb on + strand, within RR41_RS16185 at 3362.735 kb on + strand, within RR41_RS16185 at 3362.765 kb on + strand, within RR41_RS16185 at 3362.766 kb on - strand, within RR41_RS16185 at 3362.769 kb on + strand, within RR41_RS16185 at 3362.770 kb on - strand, within RR41_RS16185 at 3362.855 kb on + strand, within RR41_RS16185 at 3362.857 kb on + strand, within RR41_RS16185 at 3362.857 kb on + strand, within RR41_RS16185 at 3362.881 kb on - strand, within RR41_RS16185 at 3362.891 kb on + strand, within RR41_RS16185 at 3362.892 kb on - strand, within RR41_RS16185 at 3362.896 kb on - strand, within RR41_RS16185 at 3362.955 kb on + strand, within RR41_RS16185 at 3362.956 kb on - strand, within RR41_RS16185 at 3362.964 kb on + strand, within RR41_RS16185 at 3362.968 kb on - strand, within RR41_RS16185 at 3363.060 kb on - strand at 3363.060 kb on - strand at 3363.146 kb on + strand at 3363.146 kb on + strand at 3363.147 kb on - strand at 3363.155 kb on + strand at 3363.156 kb on - strand at 3363.172 kb on + strand, within RR41_RS16190 at 3363.198 kb on + strand, within RR41_RS16190 at 3363.423 kb on - strand, within RR41_RS16190 at 3363.435 kb on + strand, within RR41_RS16190 at 3363.471 kb on - strand, within RR41_RS16190 at 3363.474 kb on + strand, within RR41_RS16190 at 3363.474 kb on + strand, within RR41_RS16190 at 3363.475 kb on - strand, within RR41_RS16190 at 3363.479 kb on - strand, within RR41_RS16190 at 3363.479 kb on - strand, within RR41_RS16190 at 3363.481 kb on + strand, within RR41_RS16190 at 3363.489 kb on - strand, within RR41_RS16190 at 3363.559 kb on + strand, within RR41_RS16190 at 3363.560 kb on - strand, within RR41_RS16190 at 3363.562 kb on + strand, within RR41_RS16190 at 3363.562 kb on + strand, within RR41_RS16190 at 3363.563 kb on - strand, within RR41_RS16190 at 3363.596 kb on - strand, within RR41_RS16190 at 3363.741 kb on - strand, within RR41_RS16190 at 3363.769 kb on + strand, within RR41_RS16190 at 3363.932 kb on - strand at 3364.044 kb on - strand at 3364.044 kb on - strand at 3364.050 kb on - strand at 3364.074 kb on - strand at 3364.074 kb on - strand at 3364.079 kb on + strand at 3364.079 kb on + strand at 3364.079 kb on + strand at 3364.080 kb on - strand at 3364.080 kb on - strand at 3364.097 kb on - strand at 3364.161 kb on - strand at 3364.164 kb on + strand at 3364.164 kb on + strand at 3364.164 kb on + strand at 3364.164 kb on + strand at 3364.165 kb on - strand at 3364.209 kb on + strand, within RR41_RS16195 at 3364.344 kb on - strand, within RR41_RS16195 at 3364.344 kb on - strand, within RR41_RS16195 at 3364.385 kb on + strand, within RR41_RS16195 at 3364.394 kb on - strand, within RR41_RS16195 at 3364.397 kb on + strand, within RR41_RS16195 at 3364.397 kb on + strand, within RR41_RS16195 at 3364.398 kb on - strand, within RR41_RS16195 at 3364.463 kb on + strand, within RR41_RS16195 at 3364.486 kb on - strand, within RR41_RS16195 at 3364.486 kb on - strand, within RR41_RS16195 at 3364.513 kb on + strand, within RR41_RS16195 at 3364.523 kb on + strand, within RR41_RS16195 at 3364.530 kb on + strand, within RR41_RS16195 at 3364.552 kb on + strand, within RR41_RS16195 at 3364.582 kb on - strand, within RR41_RS16195 at 3364.607 kb on + strand, within RR41_RS16195 at 3364.669 kb on + strand, within RR41_RS16195 at 3364.669 kb on + strand, within RR41_RS16195 at 3364.669 kb on + strand, within RR41_RS16195 at 3364.673 kb on + strand, within RR41_RS16195 at 3364.673 kb on + strand, within RR41_RS16195 at 3364.674 kb on - strand, within RR41_RS16195 at 3364.717 kb on + strand, within RR41_RS16195 at 3364.775 kb on + strand, within RR41_RS16195 at 3364.819 kb on + strand, within RR41_RS16195 at 3364.820 kb on - strand, within RR41_RS16195 at 3364.820 kb on - strand, within RR41_RS16195 at 3364.875 kb on - strand at 3364.882 kb on + strand at 3364.882 kb on + strand at 3364.883 kb on - strand at 3364.898 kb on + strand at 3364.898 kb on + strand at 3364.898 kb on + strand at 3364.898 kb on + strand at 3364.899 kb on - strand at 3364.921 kb on + strand at 3364.986 kb on - strand at 3365.008 kb on + strand at 3365.008 kb on + strand at 3365.008 kb on + strand at 3365.009 kb on - strand at 3365.009 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RPMI_TSB_serum temperature; 30C remove 3,362,084 + -0.4 3,362,085 - -2.0 3,362,085 - -0.4 3,362,126 - -2.5 3,362,126 - -0.7 3,362,127 + -2.3 3,362,127 + -2.5 3,362,127 + +0.0 3,362,238 - -2.0 3,362,240 + -0.8 3,362,240 + -1.3 3,362,240 + -0.4 3,362,241 - -1.3 3,362,260 + -2.3 3,362,328 - -1.0 3,362,328 - -2.5 3,362,355 + RR41_RS16185 0.11 +0.0 3,362,356 - RR41_RS16185 0.12 +0.0 3,362,356 - RR41_RS16185 0.12 -1.1 3,362,365 - RR41_RS16185 0.13 +0.0 3,362,365 - RR41_RS16185 0.13 +1.8 3,362,466 + RR41_RS16185 0.25 -1.4 3,362,466 + RR41_RS16185 0.25 -1.0 3,362,469 - RR41_RS16185 0.25 +2.0 3,362,547 - RR41_RS16185 0.35 +2.0 3,362,623 - RR41_RS16185 0.44 -0.2 3,362,655 + RR41_RS16185 0.48 -1.2 3,362,697 + RR41_RS16185 0.54 -1.5 3,362,713 + RR41_RS16185 0.55 -1.3 3,362,731 + RR41_RS16185 0.58 -1.5 3,362,731 + RR41_RS16185 0.58 +0.6 3,362,731 + RR41_RS16185 0.58 -2.0 3,362,731 + RR41_RS16185 0.58 +1.9 3,362,732 - RR41_RS16185 0.58 +0.0 3,362,732 - RR41_RS16185 0.58 -0.2 3,362,732 - RR41_RS16185 0.58 -0.1 3,362,732 - RR41_RS16185 0.58 +1.0 3,362,732 - RR41_RS16185 0.58 -1.0 3,362,733 + RR41_RS16185 0.58 -1.4 3,362,733 + RR41_RS16185 0.58 +0.7 3,362,734 - RR41_RS16185 0.58 -1.3 3,362,735 + RR41_RS16185 0.58 -2.3 3,362,735 + RR41_RS16185 0.58 -0.4 3,362,765 + RR41_RS16185 0.62 -0.5 3,362,766 - RR41_RS16185 0.62 -1.5 3,362,769 + RR41_RS16185 0.62 -0.9 3,362,770 - RR41_RS16185 0.62 +0.0 3,362,855 + RR41_RS16185 0.73 +0.0 3,362,857 + RR41_RS16185 0.73 -2.3 3,362,857 + RR41_RS16185 0.73 +1.6 3,362,881 - RR41_RS16185 0.76 +0.6 3,362,891 + RR41_RS16185 0.77 -2.0 3,362,892 - RR41_RS16185 0.77 -2.5 3,362,896 - RR41_RS16185 0.78 +1.0 3,362,955 + RR41_RS16185 0.85 +2.6 3,362,956 - RR41_RS16185 0.85 +1.0 3,362,964 + RR41_RS16185 0.86 -1.5 3,362,968 - RR41_RS16185 0.87 +0.5 3,363,060 - -0.4 3,363,060 - -2.3 3,363,146 + -1.0 3,363,146 + +0.0 3,363,147 - -1.7 3,363,155 + -0.5 3,363,156 - +0.3 3,363,172 + RR41_RS16190 0.10 -2.0 3,363,198 + RR41_RS16190 0.13 +0.0 3,363,423 - RR41_RS16190 0.37 -1.5 3,363,435 + RR41_RS16190 0.38 -2.3 3,363,471 - RR41_RS16190 0.42 -0.3 3,363,474 + RR41_RS16190 0.42 -1.1 3,363,474 + RR41_RS16190 0.42 -2.0 3,363,475 - RR41_RS16190 0.43 -3.4 3,363,479 - RR41_RS16190 0.43 +0.0 3,363,479 - RR41_RS16190 0.43 -1.2 3,363,481 + RR41_RS16190 0.43 +0.0 3,363,489 - RR41_RS16190 0.44 -2.0 3,363,559 + RR41_RS16190 0.51 +0.0 3,363,560 - RR41_RS16190 0.52 -2.0 3,363,562 + RR41_RS16190 0.52 -1.2 3,363,562 + RR41_RS16190 0.52 -1.0 3,363,563 - RR41_RS16190 0.52 +0.7 3,363,596 - RR41_RS16190 0.55 +1.0 3,363,741 - RR41_RS16190 0.71 +0.0 3,363,769 + RR41_RS16190 0.74 +0.0 3,363,932 - +0.0 3,364,044 - -0.2 3,364,044 - -0.4 3,364,050 - -1.0 3,364,074 - -2.0 3,364,074 - -1.0 3,364,079 + +0.0 3,364,079 + +0.6 3,364,079 + -1.5 3,364,080 - -0.6 3,364,080 - +0.0 3,364,097 - +0.0 3,364,161 - -1.5 3,364,164 + -0.5 3,364,164 + +0.0 3,364,164 + -0.3 3,364,164 + -1.5 3,364,165 - -1.8 3,364,209 + RR41_RS16195 0.13 +1.5 3,364,344 - RR41_RS16195 0.29 +1.4 3,364,344 - RR41_RS16195 0.29 +0.0 3,364,385 + RR41_RS16195 0.33 +0.2 3,364,394 - RR41_RS16195 0.35 +1.9 3,364,397 + RR41_RS16195 0.35 +0.0 3,364,397 + RR41_RS16195 0.35 +0.5 3,364,398 - RR41_RS16195 0.35 -0.2 3,364,463 + RR41_RS16195 0.43 -1.0 3,364,486 - RR41_RS16195 0.45 +1.9 3,364,486 - RR41_RS16195 0.45 +1.4 3,364,513 + RR41_RS16195 0.49 -2.0 3,364,523 + RR41_RS16195 0.50 -2.0 3,364,530 + RR41_RS16195 0.51 +0.0 3,364,552 + RR41_RS16195 0.53 +0.7 3,364,582 - RR41_RS16195 0.57 -1.5 3,364,607 + RR41_RS16195 0.60 -2.5 3,364,669 + RR41_RS16195 0.67 +1.2 3,364,669 + RR41_RS16195 0.67 +1.1 3,364,669 + RR41_RS16195 0.67 -0.5 3,364,673 + RR41_RS16195 0.68 +1.6 3,364,673 + RR41_RS16195 0.68 -1.0 3,364,674 - RR41_RS16195 0.68 -0.3 3,364,717 + RR41_RS16195 0.73 +1.0 3,364,775 + RR41_RS16195 0.80 -1.5 3,364,819 + RR41_RS16195 0.85 +0.9 3,364,820 - RR41_RS16195 0.85 +0.9 3,364,820 - RR41_RS16195 0.85 -2.5 3,364,875 - -3.1 3,364,882 + -1.0 3,364,882 + -1.0 3,364,883 - -2.0 3,364,898 + -0.5 3,364,898 + +0.0 3,364,898 + -1.8 3,364,898 + -3.0 3,364,899 - +0.0 3,364,921 + -1.0 3,364,986 - -0.5 3,365,008 + -1.7 3,365,008 + -2.0 3,365,008 + -1.2 3,365,009 - +0.0 3,365,009 - -2.8
Or see this region's nucleotide sequence