Experiment: RPMI_TSB_serum temperature; 30C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR41_RS16040 and RR41_RS16045 are separated by 22 nucleotides RR41_RS16045 and RR41_RS16050 are separated by 3 nucleotides RR41_RS16050 and RR41_RS16055 are separated by 45 nucleotides RR41_RS16055 and RR41_RS16060 overlap by 4 nucleotides
RR41_RS16040: RR41_RS16040 - RNase adapter RapZ, at 3,329,915 to 3,330,766
_RS16040
RR41_RS16045: RR41_RS16045 - pantoate--beta-alanine ligase, at 3,330,789 to 3,331,637
_RS16045
RR41_RS16050: RR41_RS16050 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 3,331,641 to 3,332,450
_RS16050
RR41_RS16055: RR41_RS16055 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase, at 3,332,496 to 3,332,978
_RS16055
RR41_RS16060: RR41_RS16060 - polynucleotide adenylyltransferase PcnB, at 3,332,975 to 3,334,438
_RS16060
Position (kb)
3331
3332
3333 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3330.688 kb on - strand at 3330.701 kb on + strand at 3330.704 kb on + strand at 3330.752 kb on - strand at 3330.752 kb on - strand at 3330.755 kb on + strand at 3330.755 kb on + strand at 3330.756 kb on - strand at 3330.757 kb on + strand at 3330.783 kb on + strand at 3330.786 kb on - strand at 3330.786 kb on - strand at 3330.787 kb on + strand at 3330.788 kb on - strand at 3330.800 kb on + strand at 3331.798 kb on - strand, within RR41_RS16050 at 3331.809 kb on + strand, within RR41_RS16050 at 3331.896 kb on + strand, within RR41_RS16050 at 3331.994 kb on + strand, within RR41_RS16050 at 3332.052 kb on + strand, within RR41_RS16050 at 3332.439 kb on + strand at 3332.482 kb on + strand at 3332.482 kb on + strand at 3332.483 kb on - strand at 3332.485 kb on + strand at 3332.486 kb on - strand at 3332.497 kb on + strand at 3332.521 kb on - strand at 3333.098 kb on - strand at 3333.098 kb on - strand at 3333.244 kb on + strand, within RR41_RS16060 at 3333.245 kb on - strand, within RR41_RS16060 at 3333.245 kb on - strand, within RR41_RS16060 at 3333.245 kb on - strand, within RR41_RS16060 at 3333.245 kb on - strand, within RR41_RS16060 at 3333.245 kb on - strand, within RR41_RS16060 at 3333.247 kb on - strand, within RR41_RS16060 at 3333.247 kb on - strand, within RR41_RS16060 at 3333.350 kb on + strand, within RR41_RS16060 at 3333.350 kb on + strand, within RR41_RS16060 at 3333.351 kb on - strand, within RR41_RS16060 at 3333.351 kb on - strand, within RR41_RS16060 at 3333.351 kb on - strand, within RR41_RS16060 at 3333.351 kb on - strand, within RR41_RS16060 at 3333.353 kb on + strand, within RR41_RS16060 at 3333.354 kb on - strand, within RR41_RS16060 at 3333.354 kb on - strand, within RR41_RS16060 at 3333.354 kb on - strand, within RR41_RS16060
Per-strain Table
Position Strand Gene LocusTag Fraction RPMI_TSB_serum temperature; 30C remove 3,330,688 - -3.1 3,330,701 + +2.2 3,330,704 + -2.7 3,330,752 - -2.3 3,330,752 - -0.9 3,330,755 + -0.7 3,330,755 + -2.3 3,330,756 - -1.5 3,330,757 + +0.1 3,330,783 + -1.5 3,330,786 - +0.3 3,330,786 - +1.1 3,330,787 + -2.7 3,330,788 - +0.1 3,330,800 + +0.4 3,331,798 - RR41_RS16050 0.19 +0.1 3,331,809 + RR41_RS16050 0.21 -3.7 3,331,896 + RR41_RS16050 0.31 -0.9 3,331,994 + RR41_RS16050 0.44 +0.1 3,332,052 + RR41_RS16050 0.51 -0.9 3,332,439 + +0.1 3,332,482 + +0.7 3,332,482 + +0.1 3,332,483 - -1.5 3,332,485 + -3.1 3,332,486 - +0.9 3,332,497 + +1.3 3,332,521 - -2.5 3,333,098 - -1.9 3,333,098 - -0.5 3,333,244 + RR41_RS16060 0.18 +1.1 3,333,245 - RR41_RS16060 0.18 -0.5 3,333,245 - RR41_RS16060 0.18 -1.5 3,333,245 - RR41_RS16060 0.18 +0.8 3,333,245 - RR41_RS16060 0.18 -0.9 3,333,245 - RR41_RS16060 0.18 -0.9 3,333,247 - RR41_RS16060 0.19 -0.5 3,333,247 - RR41_RS16060 0.19 -0.9 3,333,350 + RR41_RS16060 0.26 -1.9 3,333,350 + RR41_RS16060 0.26 -1.9 3,333,351 - RR41_RS16060 0.26 -2.3 3,333,351 - RR41_RS16060 0.26 -3.3 3,333,351 - RR41_RS16060 0.26 -3.1 3,333,351 - RR41_RS16060 0.26 -0.7 3,333,353 + RR41_RS16060 0.26 -1.5 3,333,354 - RR41_RS16060 0.26 -1.9 3,333,354 - RR41_RS16060 0.26 -1.5 3,333,354 - RR41_RS16060 0.26 -2.6
Or see this region's nucleotide sequence