Experiment: RPMI_TSB_serum temperature; 30C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR41_RS05340 and RR41_RS05345 are separated by 102 nucleotides RR41_RS05345 and RR41_RS05350 are separated by 114 nucleotides RR41_RS05350 and RR41_RS05355 are separated by 101 nucleotides
RR41_RS05340: RR41_RS05340 - hypothetical protein, at 1,108,278 to 1,108,649
_RS05340
RR41_RS05345: RR41_RS05345 - 5'/3'-nucleotidase SurE, at 1,108,752 to 1,109,519
_RS05345
RR41_RS05350: RR41_RS05350 - peptidoglycan DD-metalloendopeptidase family protein, at 1,109,634 to 1,110,464
_RS05350
RR41_RS05355: RR41_RS05355 - LysR family transcriptional regulator, at 1,110,566 to 1,111,495
_RS05355
Position (kb)
1109
1110
1111 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1108.671 kb on + strand at 1108.681 kb on + strand at 1108.686 kb on - strand at 1108.686 kb on - strand at 1108.686 kb on - strand at 1108.691 kb on + strand at 1108.762 kb on + strand at 1108.762 kb on + strand at 1108.783 kb on + strand at 1108.784 kb on - strand at 1108.798 kb on + strand at 1108.799 kb on - strand at 1108.885 kb on + strand, within RR41_RS05345 at 1108.904 kb on - strand, within RR41_RS05345 at 1108.930 kb on + strand, within RR41_RS05345 at 1108.930 kb on + strand, within RR41_RS05345 at 1108.930 kb on + strand, within RR41_RS05345 at 1108.931 kb on - strand, within RR41_RS05345 at 1108.941 kb on - strand, within RR41_RS05345 at 1108.966 kb on - strand, within RR41_RS05345 at 1108.966 kb on - strand, within RR41_RS05345 at 1108.969 kb on + strand, within RR41_RS05345 at 1109.137 kb on + strand, within RR41_RS05345 at 1109.137 kb on + strand, within RR41_RS05345 at 1109.137 kb on + strand, within RR41_RS05345 at 1109.137 kb on + strand, within RR41_RS05345 at 1109.138 kb on - strand, within RR41_RS05345 at 1109.138 kb on - strand, within RR41_RS05345 at 1109.138 kb on - strand, within RR41_RS05345 at 1109.138 kb on - strand, within RR41_RS05345 at 1109.138 kb on - strand, within RR41_RS05345 at 1109.145 kb on + strand, within RR41_RS05345 at 1109.146 kb on - strand, within RR41_RS05345 at 1109.186 kb on - strand, within RR41_RS05345 at 1109.196 kb on - strand, within RR41_RS05345 at 1109.209 kb on + strand, within RR41_RS05345 at 1109.210 kb on - strand, within RR41_RS05345 at 1109.237 kb on + strand, within RR41_RS05345 at 1109.243 kb on + strand, within RR41_RS05345 at 1109.244 kb on - strand, within RR41_RS05345 at 1109.246 kb on + strand, within RR41_RS05345 at 1109.258 kb on - strand, within RR41_RS05345 at 1109.258 kb on - strand, within RR41_RS05345 at 1109.258 kb on - strand, within RR41_RS05345 at 1109.282 kb on + strand, within RR41_RS05345 at 1109.282 kb on + strand, within RR41_RS05345 at 1109.283 kb on - strand, within RR41_RS05345 at 1109.287 kb on - strand, within RR41_RS05345 at 1109.309 kb on + strand, within RR41_RS05345 at 1109.309 kb on + strand, within RR41_RS05345 at 1109.329 kb on + strand, within RR41_RS05345 at 1109.330 kb on - strand, within RR41_RS05345 at 1109.330 kb on - strand, within RR41_RS05345 at 1109.330 kb on - strand, within RR41_RS05345 at 1109.337 kb on - strand, within RR41_RS05345 at 1109.380 kb on + strand, within RR41_RS05345 at 1109.402 kb on + strand, within RR41_RS05345 at 1109.411 kb on - strand, within RR41_RS05345 at 1109.411 kb on - strand, within RR41_RS05345 at 1109.492 kb on - strand at 1109.520 kb on + strand at 1109.520 kb on + strand at 1109.520 kb on + strand at 1109.537 kb on + strand at 1109.540 kb on - strand at 1109.619 kb on + strand at 1109.641 kb on + strand at 1109.707 kb on + strand at 1109.747 kb on + strand, within RR41_RS05350 at 1109.759 kb on + strand, within RR41_RS05350 at 1109.765 kb on + strand, within RR41_RS05350 at 1109.765 kb on + strand, within RR41_RS05350 at 1109.766 kb on - strand, within RR41_RS05350 at 1109.766 kb on - strand, within RR41_RS05350 at 1109.793 kb on + strand, within RR41_RS05350 at 1109.793 kb on + strand, within RR41_RS05350 at 1109.821 kb on + strand, within RR41_RS05350 at 1109.828 kb on + strand, within RR41_RS05350 at 1109.901 kb on + strand, within RR41_RS05350 at 1109.901 kb on + strand, within RR41_RS05350 at 1110.014 kb on + strand, within RR41_RS05350 at 1110.026 kb on + strand, within RR41_RS05350 at 1110.026 kb on + strand, within RR41_RS05350 at 1110.068 kb on + strand, within RR41_RS05350 at 1110.069 kb on - strand, within RR41_RS05350 at 1110.229 kb on + strand, within RR41_RS05350 at 1110.463 kb on - strand at 1110.467 kb on - strand at 1110.475 kb on - strand at 1110.480 kb on + strand at 1110.493 kb on - strand at 1110.493 kb on - strand at 1110.494 kb on + strand at 1110.497 kb on + strand at 1110.498 kb on - strand at 1110.524 kb on + strand at 1110.549 kb on + strand at 1110.565 kb on + strand at 1110.570 kb on + strand at 1110.625 kb on + strand at 1110.626 kb on - strand at 1110.645 kb on + strand at 1110.645 kb on + strand at 1110.652 kb on + strand at 1110.705 kb on + strand, within RR41_RS05355 at 1110.705 kb on + strand, within RR41_RS05355 at 1110.706 kb on - strand, within RR41_RS05355 at 1110.737 kb on - strand, within RR41_RS05355 at 1110.890 kb on - strand, within RR41_RS05355 at 1110.890 kb on - strand, within RR41_RS05355 at 1110.901 kb on - strand, within RR41_RS05355 at 1110.930 kb on + strand, within RR41_RS05355 at 1110.959 kb on + strand, within RR41_RS05355 at 1110.960 kb on - strand, within RR41_RS05355 at 1110.961 kb on + strand, within RR41_RS05355 at 1110.962 kb on - strand, within RR41_RS05355 at 1111.009 kb on + strand, within RR41_RS05355 at 1111.018 kb on - strand, within RR41_RS05355 at 1111.082 kb on + strand, within RR41_RS05355 at 1111.122 kb on + strand, within RR41_RS05355 at 1111.144 kb on + strand, within RR41_RS05355 at 1111.240 kb on + strand, within RR41_RS05355 at 1111.241 kb on - strand, within RR41_RS05355 at 1111.245 kb on + strand, within RR41_RS05355 at 1111.299 kb on - strand, within RR41_RS05355 at 1111.302 kb on + strand, within RR41_RS05355 at 1111.302 kb on + strand, within RR41_RS05355 at 1111.302 kb on + strand, within RR41_RS05355 at 1111.303 kb on - strand, within RR41_RS05355 at 1111.377 kb on + strand, within RR41_RS05355 at 1111.377 kb on + strand, within RR41_RS05355 at 1111.378 kb on - strand, within RR41_RS05355 at 1111.380 kb on - strand, within RR41_RS05355
Per-strain Table
Position Strand Gene LocusTag Fraction RPMI_TSB_serum temperature; 30C remove 1,108,671 + -1.7 1,108,681 + +1.8 1,108,686 - +0.5 1,108,686 - -1.5 1,108,686 - +0.3 1,108,691 + -0.7 1,108,762 + +0.8 1,108,762 + -2.1 1,108,783 + -2.3 1,108,784 - -0.3 1,108,798 + -1.9 1,108,799 - -2.5 1,108,885 + RR41_RS05345 0.17 +0.3 1,108,904 - RR41_RS05345 0.20 -1.3 1,108,930 + RR41_RS05345 0.23 -1.1 1,108,930 + RR41_RS05345 0.23 -2.3 1,108,930 + RR41_RS05345 0.23 +0.8 1,108,931 - RR41_RS05345 0.23 +3.4 1,108,941 - RR41_RS05345 0.25 +0.6 1,108,966 - RR41_RS05345 0.28 -0.6 1,108,966 - RR41_RS05345 0.28 -1.7 1,108,969 + RR41_RS05345 0.28 +1.5 1,109,137 + RR41_RS05345 0.50 -1.7 1,109,137 + RR41_RS05345 0.50 +0.3 1,109,137 + RR41_RS05345 0.50 -0.5 1,109,137 + RR41_RS05345 0.50 +2.3 1,109,138 - RR41_RS05345 0.50 -0.7 1,109,138 - RR41_RS05345 0.50 +1.5 1,109,138 - RR41_RS05345 0.50 -1.7 1,109,138 - RR41_RS05345 0.50 -0.7 1,109,138 - RR41_RS05345 0.50 -1.7 1,109,145 + RR41_RS05345 0.51 -2.5 1,109,146 - RR41_RS05345 0.51 -1.3 1,109,186 - RR41_RS05345 0.57 -1.7 1,109,196 - RR41_RS05345 0.58 +0.5 1,109,209 + RR41_RS05345 0.60 +1.3 1,109,210 - RR41_RS05345 0.60 +0.3 1,109,237 + RR41_RS05345 0.63 +0.3 1,109,243 + RR41_RS05345 0.64 -0.7 1,109,244 - RR41_RS05345 0.64 -1.7 1,109,246 + RR41_RS05345 0.64 +0.4 1,109,258 - RR41_RS05345 0.66 -2.9 1,109,258 - RR41_RS05345 0.66 -2.1 1,109,258 - RR41_RS05345 0.66 -0.7 1,109,282 + RR41_RS05345 0.69 -2.1 1,109,282 + RR41_RS05345 0.69 -0.6 1,109,283 - RR41_RS05345 0.69 -2.5 1,109,287 - RR41_RS05345 0.70 -0.3 1,109,309 + RR41_RS05345 0.73 -2.7 1,109,309 + RR41_RS05345 0.73 +1.3 1,109,329 + RR41_RS05345 0.75 +0.3 1,109,330 - RR41_RS05345 0.75 -2.1 1,109,330 - RR41_RS05345 0.75 -0.7 1,109,330 - RR41_RS05345 0.75 +2.8 1,109,337 - RR41_RS05345 0.76 +0.3 1,109,380 + RR41_RS05345 0.82 -1.3 1,109,402 + RR41_RS05345 0.85 +0.6 1,109,411 - RR41_RS05345 0.86 +0.0 1,109,411 - RR41_RS05345 0.86 +0.3 1,109,492 - -3.6 1,109,520 + -0.2 1,109,520 + -0.7 1,109,520 + -0.7 1,109,537 + -0.2 1,109,540 - +0.0 1,109,619 + -0.4 1,109,641 + -2.1 1,109,707 + +0.3 1,109,747 + RR41_RS05350 0.14 +2.4 1,109,759 + RR41_RS05350 0.15 -1.7 1,109,765 + RR41_RS05350 0.16 -2.1 1,109,765 + RR41_RS05350 0.16 -1.7 1,109,766 - RR41_RS05350 0.16 -0.7 1,109,766 - RR41_RS05350 0.16 -1.7 1,109,793 + RR41_RS05350 0.19 -2.5 1,109,793 + RR41_RS05350 0.19 -1.7 1,109,821 + RR41_RS05350 0.23 -2.5 1,109,828 + RR41_RS05350 0.23 +0.3 1,109,901 + RR41_RS05350 0.32 -1.7 1,109,901 + RR41_RS05350 0.32 -2.5 1,110,014 + RR41_RS05350 0.46 -1.3 1,110,026 + RR41_RS05350 0.47 -0.3 1,110,026 + RR41_RS05350 0.47 -2.3 1,110,068 + RR41_RS05350 0.52 +0.3 1,110,069 - RR41_RS05350 0.52 +3.1 1,110,229 + RR41_RS05350 0.72 +0.3 1,110,463 - -0.7 1,110,467 - +0.9 1,110,475 - -2.3 1,110,480 + -1.7 1,110,493 - -1.0 1,110,493 - -0.7 1,110,494 + +0.9 1,110,497 + -0.2 1,110,498 - -2.1 1,110,524 + -1.1 1,110,549 + -1.1 1,110,565 + -0.7 1,110,570 + -0.7 1,110,625 + +0.9 1,110,626 - -1.3 1,110,645 + -1.3 1,110,645 + +0.3 1,110,652 + +1.9 1,110,705 + RR41_RS05355 0.15 -0.7 1,110,705 + RR41_RS05355 0.15 +1.3 1,110,706 - RR41_RS05355 0.15 +0.0 1,110,737 - RR41_RS05355 0.18 -2.5 1,110,890 - RR41_RS05355 0.35 +1.3 1,110,890 - RR41_RS05355 0.35 -0.8 1,110,901 - RR41_RS05355 0.36 +1.9 1,110,930 + RR41_RS05355 0.39 -2.5 1,110,959 + RR41_RS05355 0.42 -1.3 1,110,960 - RR41_RS05355 0.42 -1.7 1,110,961 + RR41_RS05355 0.42 +0.7 1,110,962 - RR41_RS05355 0.43 -1.1 1,111,009 + RR41_RS05355 0.48 -1.1 1,111,018 - RR41_RS05355 0.49 +2.6 1,111,082 + RR41_RS05355 0.55 -0.4 1,111,122 + RR41_RS05355 0.60 +0.9 1,111,144 + RR41_RS05355 0.62 -1.3 1,111,240 + RR41_RS05355 0.72 -0.7 1,111,241 - RR41_RS05355 0.73 +1.9 1,111,245 + RR41_RS05355 0.73 -1.3 1,111,299 - RR41_RS05355 0.79 +0.9 1,111,302 + RR41_RS05355 0.79 +2.1 1,111,302 + RR41_RS05355 0.79 -1.7 1,111,302 + RR41_RS05355 0.79 +1.9 1,111,303 - RR41_RS05355 0.79 -0.1 1,111,377 + RR41_RS05355 0.87 -2.5 1,111,377 + RR41_RS05355 0.87 +1.5 1,111,378 - RR41_RS05355 0.87 -1.3 1,111,380 - RR41_RS05355 0.88 -2.1
Or see this region's nucleotide sequence