Experiment: RPMI_TSB_serum temperature; 30C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR41_RS04660 and RR41_RS04665 are separated by 3 nucleotides RR41_RS04665 and RR41_RS04670 overlap by 1 nucleotides RR41_RS04670 and RR41_RS04675 are separated by 23 nucleotides RR41_RS04675 and RR41_RS04680 are separated by 210 nucleotides
RR41_RS04660: RR41_RS04660 - cell envelope integrity protein TolA, at 964,354 to 965,700
_RS04660
RR41_RS04665: RR41_RS04665 - protein TolR, at 965,704 to 966,156
_RS04665
RR41_RS04670: RR41_RS04670 - protein TolQ, at 966,156 to 966,854
_RS04670
RR41_RS04675: RR41_RS04675 - tol-pal system-associated acyl-CoA thioesterase, at 966,878 to 967,285
_RS04675
RR41_RS04680: RR41_RS04680 - M20 family metallopeptidase, at 967,496 to 968,848
_RS04680
Position (kb) 966
967 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 965.288 kb on - strand, within RR41_RS04660 at 965.314 kb on + strand, within RR41_RS04660 at 965.337 kb on + strand, within RR41_RS04660 at 965.470 kb on + strand, within RR41_RS04660 at 965.471 kb on - strand, within RR41_RS04660 at 965.471 kb on - strand, within RR41_RS04660 at 965.498 kb on - strand, within RR41_RS04660 at 965.537 kb on - strand, within RR41_RS04660 at 965.551 kb on - strand, within RR41_RS04660 at 965.659 kb on + strand at 965.674 kb on - strand at 965.694 kb on + strand at 965.705 kb on + strand at 965.706 kb on - strand at 965.756 kb on - strand, within RR41_RS04665 at 965.773 kb on + strand, within RR41_RS04665 at 965.800 kb on - strand, within RR41_RS04665 at 965.820 kb on + strand, within RR41_RS04665 at 965.901 kb on + strand, within RR41_RS04665 at 965.909 kb on + strand, within RR41_RS04665 at 965.918 kb on + strand, within RR41_RS04665 at 965.918 kb on + strand, within RR41_RS04665 at 965.927 kb on + strand, within RR41_RS04665 at 965.928 kb on - strand, within RR41_RS04665 at 965.931 kb on + strand, within RR41_RS04665 at 965.991 kb on + strand, within RR41_RS04665 at 965.991 kb on + strand, within RR41_RS04665 at 966.015 kb on + strand, within RR41_RS04665 at 966.074 kb on - strand, within RR41_RS04665 at 966.084 kb on - strand, within RR41_RS04665 at 966.300 kb on + strand, within RR41_RS04670 at 966.430 kb on + strand, within RR41_RS04670 at 966.431 kb on - strand, within RR41_RS04670 at 966.431 kb on - strand, within RR41_RS04670 at 966.465 kb on + strand, within RR41_RS04670 at 966.472 kb on + strand, within RR41_RS04670 at 966.511 kb on - strand, within RR41_RS04670 at 966.515 kb on + strand, within RR41_RS04670 at 966.545 kb on + strand, within RR41_RS04670 at 966.545 kb on + strand, within RR41_RS04670 at 966.546 kb on - strand, within RR41_RS04670 at 966.582 kb on + strand, within RR41_RS04670 at 966.583 kb on - strand, within RR41_RS04670 at 966.583 kb on - strand, within RR41_RS04670 at 966.650 kb on + strand, within RR41_RS04670 at 966.650 kb on + strand, within RR41_RS04670 at 966.651 kb on - strand, within RR41_RS04670 at 966.714 kb on - strand, within RR41_RS04670 at 966.723 kb on - strand, within RR41_RS04670 at 966.723 kb on - strand, within RR41_RS04670 at 966.723 kb on - strand, within RR41_RS04670 at 966.723 kb on - strand, within RR41_RS04670 at 966.723 kb on - strand, within RR41_RS04670 at 966.763 kb on - strand, within RR41_RS04670 at 966.808 kb on - strand at 966.808 kb on - strand at 966.854 kb on + strand at 966.855 kb on - strand at 966.855 kb on - strand at 966.855 kb on - strand at 966.858 kb on + strand at 966.858 kb on + strand at 966.858 kb on + strand at 966.858 kb on + strand at 966.858 kb on + strand at 966.858 kb on + strand at 966.859 kb on - strand at 966.859 kb on - strand at 966.859 kb on - strand at 966.859 kb on - strand at 966.862 kb on + strand at 966.863 kb on - strand at 967.312 kb on - strand at 967.382 kb on - strand at 967.409 kb on + strand at 967.409 kb on + strand at 967.410 kb on - strand at 967.410 kb on - strand at 967.410 kb on - strand at 967.412 kb on + strand at 967.412 kb on + strand at 967.413 kb on - strand at 967.413 kb on - strand at 967.413 kb on - strand at 967.420 kb on - strand at 967.460 kb on + strand at 967.476 kb on - strand at 967.476 kb on - strand at 967.476 kb on - strand at 967.486 kb on + strand at 967.486 kb on + strand at 967.487 kb on - strand at 967.487 kb on - strand at 967.487 kb on - strand at 967.492 kb on + strand at 967.493 kb on - strand at 967.559 kb on + strand at 967.559 kb on + strand at 967.560 kb on - strand at 967.560 kb on - strand at 967.560 kb on - strand at 967.561 kb on + strand at 967.561 kb on + strand at 967.561 kb on + strand at 967.562 kb on - strand at 967.562 kb on - strand at 967.565 kb on + strand at 967.565 kb on + strand at 967.565 kb on + strand at 967.584 kb on - strand at 967.591 kb on + strand at 967.598 kb on + strand at 967.598 kb on + strand at 967.599 kb on - strand at 967.599 kb on - strand at 967.602 kb on - strand at 967.603 kb on + strand at 967.603 kb on + strand at 967.603 kb on + strand at 967.603 kb on + strand at 967.604 kb on - strand at 967.637 kb on + strand, within RR41_RS04680 at 967.637 kb on + strand, within RR41_RS04680 at 967.665 kb on - strand, within RR41_RS04680 at 967.688 kb on + strand, within RR41_RS04680 at 967.689 kb on - strand, within RR41_RS04680 at 967.791 kb on - strand, within RR41_RS04680 at 967.804 kb on + strand, within RR41_RS04680 at 967.813 kb on + strand, within RR41_RS04680 at 967.813 kb on + strand, within RR41_RS04680 at 967.845 kb on + strand, within RR41_RS04680
Per-strain Table
Position Strand Gene LocusTag Fraction RPMI_TSB_serum temperature; 30C remove 965,288 - RR41_RS04660 0.69 -1.3 965,314 + RR41_RS04660 0.71 -0.2 965,337 + RR41_RS04660 0.73 -0.7 965,470 + RR41_RS04660 0.83 +0.3 965,471 - RR41_RS04660 0.83 -2.7 965,471 - RR41_RS04660 0.83 -3.3 965,498 - RR41_RS04660 0.85 -1.7 965,537 - RR41_RS04660 0.88 -2.2 965,551 - RR41_RS04660 0.89 -2.6 965,659 + -0.7 965,674 - -3.2 965,694 + -0.7 965,705 + -0.5 965,706 - -2.3 965,756 - RR41_RS04665 0.11 -2.7 965,773 + RR41_RS04665 0.15 -0.7 965,800 - RR41_RS04665 0.21 -0.7 965,820 + RR41_RS04665 0.26 +1.3 965,901 + RR41_RS04665 0.43 -1.7 965,909 + RR41_RS04665 0.45 -0.5 965,918 + RR41_RS04665 0.47 +0.3 965,918 + RR41_RS04665 0.47 -0.7 965,927 + RR41_RS04665 0.49 -2.1 965,928 - RR41_RS04665 0.49 -1.3 965,931 + RR41_RS04665 0.50 +0.3 965,991 + RR41_RS04665 0.63 -0.7 965,991 + RR41_RS04665 0.63 -0.7 966,015 + RR41_RS04665 0.69 -3.3 966,074 - RR41_RS04665 0.82 -0.7 966,084 - RR41_RS04665 0.84 -2.3 966,300 + RR41_RS04670 0.21 +1.3 966,430 + RR41_RS04670 0.39 -1.7 966,431 - RR41_RS04670 0.39 -0.7 966,431 - RR41_RS04670 0.39 -1.7 966,465 + RR41_RS04670 0.44 -1.3 966,472 + RR41_RS04670 0.45 -0.7 966,511 - RR41_RS04670 0.51 -3.2 966,515 + RR41_RS04670 0.51 -0.1 966,545 + RR41_RS04670 0.56 -1.3 966,545 + RR41_RS04670 0.56 -0.7 966,546 - RR41_RS04670 0.56 -0.7 966,582 + RR41_RS04670 0.61 +0.3 966,583 - RR41_RS04670 0.61 -2.3 966,583 - RR41_RS04670 0.61 -2.3 966,650 + RR41_RS04670 0.71 -2.3 966,650 + RR41_RS04670 0.71 +0.3 966,651 - RR41_RS04670 0.71 +0.3 966,714 - RR41_RS04670 0.80 -0.1 966,723 - RR41_RS04670 0.81 -2.7 966,723 - RR41_RS04670 0.81 +1.8 966,723 - RR41_RS04670 0.81 +0.3 966,723 - RR41_RS04670 0.81 -2.6 966,723 - RR41_RS04670 0.81 -0.5 966,763 - RR41_RS04670 0.87 -1.7 966,808 - -0.8 966,808 - -1.1 966,854 + +0.7 966,855 - -0.7 966,855 - -0.7 966,855 - -0.7 966,858 + +1.8 966,858 + -0.7 966,858 + -1.1 966,858 + +1.3 966,858 + +0.8 966,858 + -0.7 966,859 - -0.7 966,859 - +1.3 966,859 - -0.7 966,859 - +1.5 966,862 + -3.1 966,863 - -2.6 967,312 - -0.4 967,382 - +1.0 967,409 + +2.3 967,409 + -0.0 967,410 - +1.6 967,410 - -2.6 967,410 - -1.3 967,412 + +0.3 967,412 + +0.3 967,413 - +1.8 967,413 - -0.7 967,413 - -0.3 967,420 - +0.1 967,460 + -1.3 967,476 - +1.1 967,476 - +0.3 967,476 - -1.7 967,486 + +0.1 967,486 + -1.3 967,487 - +1.8 967,487 - +1.0 967,487 - +1.0 967,492 + +0.3 967,493 - +0.3 967,559 + +1.4 967,559 + -0.7 967,560 - -1.7 967,560 - -2.3 967,560 - -0.6 967,561 + +0.3 967,561 + +0.3 967,561 + +1.9 967,562 - -3.1 967,562 - -2.6 967,565 + +0.8 967,565 + +0.3 967,565 + +0.3 967,584 - -1.3 967,591 + -2.6 967,598 + +0.3 967,598 + +0.3 967,599 - -1.3 967,599 - -0.7 967,602 - +2.0 967,603 + +1.8 967,603 + +1.6 967,603 + -2.7 967,603 + +1.0 967,604 - -2.3 967,637 + RR41_RS04680 0.10 +1.1 967,637 + RR41_RS04680 0.10 -2.7 967,665 - RR41_RS04680 0.12 +0.3 967,688 + RR41_RS04680 0.14 -1.2 967,689 - RR41_RS04680 0.14 +2.3 967,791 - RR41_RS04680 0.22 -2.1 967,804 + RR41_RS04680 0.23 +4.0 967,813 + RR41_RS04680 0.23 +3.3 967,813 + RR41_RS04680 0.23 +2.3 967,845 + RR41_RS04680 0.26 -1.3
Or see this region's nucleotide sequence