Experiment: RPMI_TSB_serum temperature; 30C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR41_RS02855 and RR41_RS02860 are separated by 159 nucleotides RR41_RS02860 and RR41_RS02865 are separated by 91 nucleotides RR41_RS02865 and RR41_RS02870 are separated by 51 nucleotides
RR41_RS02855: RR41_RS02855 - tyrosine-type recombinase/integrase, at 580,344 to 581,621
_RS02855
RR41_RS02860: RR41_RS02860 - YajQ family cyclic di-GMP-binding protein, at 581,781 to 582,269
_RS02860
RR41_RS02865: RR41_RS02865 - sulfurtransferase, at 582,361 to 582,675
_RS02865
RR41_RS02870: RR41_RS02870 - FAD-dependent 5-carboxymethylaminomethyl-2-thiouridine(34) oxidoreductase MnmC, at 582,727 to 584,598
_RS02870
Position (kb)
582
583 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 581.411 kb on + strand, within RR41_RS02855 at 581.412 kb on - strand, within RR41_RS02855 at 581.417 kb on + strand, within RR41_RS02855 at 581.425 kb on + strand, within RR41_RS02855 at 581.425 kb on + strand, within RR41_RS02855 at 581.429 kb on - strand, within RR41_RS02855 at 581.449 kb on - strand, within RR41_RS02855 at 581.471 kb on + strand, within RR41_RS02855 at 581.472 kb on - strand, within RR41_RS02855 at 581.472 kb on - strand, within RR41_RS02855 at 581.472 kb on - strand, within RR41_RS02855 at 581.472 kb on - strand, within RR41_RS02855 at 581.474 kb on + strand, within RR41_RS02855 at 581.475 kb on - strand, within RR41_RS02855 at 581.475 kb on - strand, within RR41_RS02855 at 581.490 kb on - strand, within RR41_RS02855 at 581.494 kb on - strand at 581.541 kb on + strand at 581.541 kb on + strand at 581.541 kb on + strand at 581.541 kb on + strand at 581.542 kb on - strand at 581.542 kb on - strand at 581.542 kb on - strand at 581.542 kb on - strand at 581.542 kb on - strand at 581.542 kb on - strand at 581.542 kb on - strand at 581.544 kb on - strand at 581.545 kb on + strand at 581.545 kb on + strand at 581.545 kb on + strand at 581.545 kb on + strand at 581.546 kb on - strand at 581.546 kb on - strand at 581.546 kb on - strand at 581.552 kb on + strand at 581.567 kb on + strand at 581.568 kb on - strand at 581.616 kb on + strand at 581.617 kb on - strand at 581.674 kb on + strand at 581.675 kb on - strand at 581.694 kb on + strand at 581.694 kb on + strand at 581.694 kb on + strand at 581.695 kb on - strand at 581.695 kb on - strand at 581.695 kb on - strand at 581.697 kb on + strand at 581.697 kb on + strand at 581.698 kb on - strand at 581.704 kb on + strand at 581.704 kb on + strand at 581.707 kb on + strand at 581.708 kb on - strand at 581.778 kb on - strand at 581.796 kb on - strand at 581.799 kb on - strand at 581.805 kb on + strand at 581.806 kb on - strand at 581.806 kb on - strand at 581.853 kb on + strand, within RR41_RS02860 at 581.871 kb on - strand, within RR41_RS02860 at 581.871 kb on - strand, within RR41_RS02860 at 581.900 kb on + strand, within RR41_RS02860 at 581.900 kb on + strand, within RR41_RS02860 at 581.901 kb on - strand, within RR41_RS02860 at 581.901 kb on - strand, within RR41_RS02860 at 581.919 kb on - strand, within RR41_RS02860 at 581.939 kb on + strand, within RR41_RS02860 at 581.940 kb on - strand, within RR41_RS02860 at 581.957 kb on + strand, within RR41_RS02860 at 581.979 kb on + strand, within RR41_RS02860 at 581.995 kb on - strand, within RR41_RS02860 at 582.061 kb on + strand, within RR41_RS02860 at 582.061 kb on + strand, within RR41_RS02860 at 582.083 kb on + strand, within RR41_RS02860 at 582.147 kb on + strand, within RR41_RS02860 at 582.213 kb on + strand, within RR41_RS02860 at 582.235 kb on - strand at 582.235 kb on - strand at 582.241 kb on - strand at 582.275 kb on - strand at 582.275 kb on - strand at 582.282 kb on + strand at 582.298 kb on - strand at 582.309 kb on + strand at 582.309 kb on + strand at 582.310 kb on - strand at 582.342 kb on + strand at 582.342 kb on + strand at 582.342 kb on + strand at 582.343 kb on - strand at 582.457 kb on - strand, within RR41_RS02865 at 582.457 kb on - strand, within RR41_RS02865 at 582.488 kb on + strand, within RR41_RS02865 at 582.488 kb on + strand, within RR41_RS02865 at 582.489 kb on - strand, within RR41_RS02865 at 582.498 kb on + strand, within RR41_RS02865 at 582.540 kb on - strand, within RR41_RS02865 at 582.540 kb on - strand, within RR41_RS02865 at 582.581 kb on + strand, within RR41_RS02865 at 582.590 kb on + strand, within RR41_RS02865 at 582.607 kb on + strand, within RR41_RS02865 at 582.607 kb on + strand, within RR41_RS02865 at 582.608 kb on - strand, within RR41_RS02865 at 582.653 kb on + strand at 582.660 kb on - strand at 582.753 kb on + strand at 582.760 kb on - strand at 582.760 kb on - strand at 582.796 kb on + strand at 582.796 kb on + strand at 582.855 kb on + strand at 582.855 kb on + strand at 582.856 kb on - strand at 583.004 kb on - strand, within RR41_RS02870 at 583.004 kb on - strand, within RR41_RS02870 at 583.004 kb on - strand, within RR41_RS02870 at 583.016 kb on + strand, within RR41_RS02870 at 583.073 kb on - strand, within RR41_RS02870 at 583.117 kb on - strand, within RR41_RS02870 at 583.140 kb on + strand, within RR41_RS02870 at 583.141 kb on - strand, within RR41_RS02870 at 583.141 kb on - strand, within RR41_RS02870 at 583.154 kb on + strand, within RR41_RS02870 at 583.155 kb on - strand, within RR41_RS02870 at 583.158 kb on + strand, within RR41_RS02870 at 583.158 kb on + strand, within RR41_RS02870 at 583.159 kb on - strand, within RR41_RS02870 at 583.159 kb on - strand, within RR41_RS02870 at 583.159 kb on - strand, within RR41_RS02870 at 583.159 kb on - strand, within RR41_RS02870 at 583.159 kb on - strand, within RR41_RS02870 at 583.160 kb on + strand, within RR41_RS02870 at 583.178 kb on - strand, within RR41_RS02870 at 583.194 kb on + strand, within RR41_RS02870 at 583.220 kb on - strand, within RR41_RS02870 at 583.249 kb on + strand, within RR41_RS02870 at 583.249 kb on + strand, within RR41_RS02870 at 583.298 kb on - strand, within RR41_RS02870 at 583.303 kb on - strand, within RR41_RS02870 at 583.303 kb on - strand, within RR41_RS02870 at 583.303 kb on - strand, within RR41_RS02870 at 583.322 kb on - strand, within RR41_RS02870 at 583.322 kb on - strand, within RR41_RS02870 at 583.381 kb on - strand, within RR41_RS02870 at 583.458 kb on - strand, within RR41_RS02870 at 583.458 kb on - strand, within RR41_RS02870 at 583.516 kb on - strand, within RR41_RS02870 at 583.618 kb on + strand, within RR41_RS02870 at 583.618 kb on + strand, within RR41_RS02870 at 583.657 kb on + strand, within RR41_RS02870 at 583.657 kb on + strand, within RR41_RS02870 at 583.668 kb on + strand, within RR41_RS02870 at 583.668 kb on + strand, within RR41_RS02870
Per-strain Table
Position Strand Gene LocusTag Fraction RPMI_TSB_serum temperature; 30C remove 581,411 + RR41_RS02855 0.83 -1.0 581,412 - RR41_RS02855 0.84 +1.0 581,417 + RR41_RS02855 0.84 +3.5 581,425 + RR41_RS02855 0.85 +0.6 581,425 + RR41_RS02855 0.85 +1.9 581,429 - RR41_RS02855 0.85 +1.6 581,449 - RR41_RS02855 0.86 -0.8 581,471 + RR41_RS02855 0.88 +0.8 581,472 - RR41_RS02855 0.88 -1.6 581,472 - RR41_RS02855 0.88 +0.7 581,472 - RR41_RS02855 0.88 -1.2 581,472 - RR41_RS02855 0.88 +0.4 581,474 + RR41_RS02855 0.88 +0.0 581,475 - RR41_RS02855 0.88 +1.4 581,475 - RR41_RS02855 0.88 +1.6 581,490 - RR41_RS02855 0.90 -1.6 581,494 - +0.6 581,541 + +0.0 581,541 + +0.0 581,541 + -0.6 581,541 + +0.0 581,542 - -1.0 581,542 - -1.0 581,542 - +1.8 581,542 - -0.6 581,542 - -3.1 581,542 - -1.6 581,542 - -1.4 581,544 - +0.3 581,545 + -1.0 581,545 + +0.0 581,545 + -2.0 581,545 + +0.7 581,546 - +0.6 581,546 - -0.7 581,546 - -1.3 581,552 + +0.4 581,567 + -0.2 581,568 - -0.3 581,616 + +0.6 581,617 - -2.6 581,674 + +2.3 581,675 - +2.3 581,694 + -1.3 581,694 + -2.3 581,694 + +3.7 581,695 - +0.7 581,695 - -2.0 581,695 - -0.4 581,697 + -1.6 581,697 + -0.1 581,698 - +0.0 581,704 + -1.0 581,704 + +0.0 581,707 + -1.0 581,708 - +0.0 581,778 - -0.5 581,796 - +0.0 581,799 - +1.5 581,805 + +2.8 581,806 - +1.3 581,806 - +0.6 581,853 + RR41_RS02860 0.15 +1.6 581,871 - RR41_RS02860 0.18 -2.8 581,871 - RR41_RS02860 0.18 -2.8 581,900 + RR41_RS02860 0.24 +1.6 581,900 + RR41_RS02860 0.24 -2.1 581,901 - RR41_RS02860 0.25 -3.0 581,901 - RR41_RS02860 0.25 +0.0 581,919 - RR41_RS02860 0.28 +1.6 581,939 + RR41_RS02860 0.32 -1.0 581,940 - RR41_RS02860 0.33 +0.6 581,957 + RR41_RS02860 0.36 -1.0 581,979 + RR41_RS02860 0.40 +0.1 581,995 - RR41_RS02860 0.44 +0.0 582,061 + RR41_RS02860 0.57 +1.0 582,061 + RR41_RS02860 0.57 -2.0 582,083 + RR41_RS02860 0.62 +0.0 582,147 + RR41_RS02860 0.75 +0.0 582,213 + RR41_RS02860 0.88 +0.0 582,235 - -1.0 582,235 - +0.0 582,241 - -1.0 582,275 - +0.7 582,275 - -1.8 582,282 + -0.3 582,298 - -1.0 582,309 + -0.8 582,309 + -2.0 582,310 - -2.3 582,342 + -0.4 582,342 + +0.2 582,342 + -0.3 582,343 - -1.6 582,457 - RR41_RS02865 0.30 -1.0 582,457 - RR41_RS02865 0.30 -2.0 582,488 + RR41_RS02865 0.40 -1.0 582,488 + RR41_RS02865 0.40 -2.4 582,489 - RR41_RS02865 0.41 +1.6 582,498 + RR41_RS02865 0.43 +0.4 582,540 - RR41_RS02865 0.57 -2.3 582,540 - RR41_RS02865 0.57 +1.0 582,581 + RR41_RS02865 0.70 -1.0 582,590 + RR41_RS02865 0.73 +0.0 582,607 + RR41_RS02865 0.78 -0.6 582,607 + RR41_RS02865 0.78 -1.5 582,608 - RR41_RS02865 0.78 -1.0 582,653 + -1.0 582,660 - +1.0 582,753 + -1.3 582,760 - -0.7 582,760 - +0.0 582,796 + +1.0 582,796 + +0.0 582,855 + +0.2 582,855 + -3.0 582,856 - +0.3 583,004 - RR41_RS02870 0.15 -1.0 583,004 - RR41_RS02870 0.15 -1.0 583,004 - RR41_RS02870 0.15 +0.8 583,016 + RR41_RS02870 0.15 +1.3 583,073 - RR41_RS02870 0.18 -0.6 583,117 - RR41_RS02870 0.21 +0.0 583,140 + RR41_RS02870 0.22 -1.5 583,141 - RR41_RS02870 0.22 -2.6 583,141 - RR41_RS02870 0.22 -1.0 583,154 + RR41_RS02870 0.23 -1.0 583,155 - RR41_RS02870 0.23 +0.6 583,158 + RR41_RS02870 0.23 +0.0 583,158 + RR41_RS02870 0.23 -0.2 583,159 - RR41_RS02870 0.23 +0.0 583,159 - RR41_RS02870 0.23 -2.3 583,159 - RR41_RS02870 0.23 +1.2 583,159 - RR41_RS02870 0.23 +0.6 583,159 - RR41_RS02870 0.23 -1.0 583,160 + RR41_RS02870 0.23 +0.4 583,178 - RR41_RS02870 0.24 +0.0 583,194 + RR41_RS02870 0.25 +1.4 583,220 - RR41_RS02870 0.26 -1.7 583,249 + RR41_RS02870 0.28 +1.6 583,249 + RR41_RS02870 0.28 +0.6 583,298 - RR41_RS02870 0.31 -0.9 583,303 - RR41_RS02870 0.31 +1.2 583,303 - RR41_RS02870 0.31 -0.2 583,303 - RR41_RS02870 0.31 -1.6 583,322 - RR41_RS02870 0.32 +1.0 583,322 - RR41_RS02870 0.32 +1.6 583,381 - RR41_RS02870 0.35 +0.6 583,458 - RR41_RS02870 0.39 -1.0 583,458 - RR41_RS02870 0.39 -0.6 583,516 - RR41_RS02870 0.42 -0.6 583,618 + RR41_RS02870 0.48 +1.2 583,618 + RR41_RS02870 0.48 -1.0 583,657 + RR41_RS02870 0.50 -0.7 583,657 + RR41_RS02870 0.50 +1.6 583,668 + RR41_RS02870 0.50 -1.6 583,668 + RR41_RS02870 0.50 -1.6
Or see this region's nucleotide sequence