Experiment: RPMI_TSB_serum temperature; 30C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR41_RS02580 and RR41_RS02585 are separated by 23 nucleotides RR41_RS02585 and RR41_RS02590 are separated by 0 nucleotides RR41_RS02590 and RR41_RS02595 are separated by 40 nucleotides RR41_RS02595 and RR41_RS02600 are separated by 84 nucleotides
RR41_RS02580: RR41_RS02580 - 4-hydroxythreonine-4-phosphate dehydrogenase PdxA, at 523,341 to 524,324
_RS02580
RR41_RS02585: RR41_RS02585 - 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA, at 524,348 to 525,160
_RS02585
RR41_RS02590: RR41_RS02590 - symmetrical bis(5'-nucleosyl)-tetraphosphatase, at 525,161 to 526,000
_RS02590
RR41_RS02595: RR41_RS02595 - DUF2147 domain-containing protein, at 526,041 to 526,484
_RS02595
RR41_RS02600: RR41_RS02600 - LrgB family protein, at 526,569 to 527,243
_RS02600
Position (kb) 525
526
527 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 524.347 kb on + strand at 524.347 kb on + strand at 524.347 kb on + strand at 524.347 kb on + strand at 524.348 kb on - strand at 524.348 kb on - strand at 524.352 kb on - strand at 524.422 kb on - strand at 524.505 kb on + strand, within RR41_RS02585 at 524.575 kb on - strand, within RR41_RS02585 at 524.620 kb on + strand, within RR41_RS02585 at 524.625 kb on + strand, within RR41_RS02585 at 524.665 kb on - strand, within RR41_RS02585 at 524.801 kb on - strand, within RR41_RS02585 at 524.801 kb on - strand, within RR41_RS02585 at 524.804 kb on - strand, within RR41_RS02585 at 524.839 kb on - strand, within RR41_RS02585 at 524.856 kb on - strand, within RR41_RS02585 at 524.902 kb on + strand, within RR41_RS02585 at 525.159 kb on - strand at 525.174 kb on - strand at 525.174 kb on - strand at 525.175 kb on + strand at 525.179 kb on + strand at 525.180 kb on - strand at 525.183 kb on + strand at 525.183 kb on + strand at 525.183 kb on + strand at 525.184 kb on - strand at 525.184 kb on - strand at 525.334 kb on - strand, within RR41_RS02590 at 525.442 kb on + strand, within RR41_RS02590 at 525.442 kb on + strand, within RR41_RS02590 at 525.501 kb on + strand, within RR41_RS02590 at 525.501 kb on + strand, within RR41_RS02590 at 525.502 kb on - strand, within RR41_RS02590 at 525.523 kb on - strand, within RR41_RS02590 at 525.540 kb on + strand, within RR41_RS02590 at 525.540 kb on + strand, within RR41_RS02590 at 525.615 kb on + strand, within RR41_RS02590 at 525.624 kb on + strand, within RR41_RS02590 at 525.688 kb on + strand, within RR41_RS02590 at 525.735 kb on + strand, within RR41_RS02590 at 525.740 kb on - strand, within RR41_RS02590 at 525.829 kb on + strand, within RR41_RS02590 at 525.830 kb on - strand, within RR41_RS02590 at 525.830 kb on - strand, within RR41_RS02590 at 525.884 kb on - strand, within RR41_RS02590 at 525.889 kb on + strand, within RR41_RS02590 at 525.889 kb on + strand, within RR41_RS02590 at 525.942 kb on + strand at 525.994 kb on + strand at 525.994 kb on + strand at 525.995 kb on - strand at 525.995 kb on - strand at 525.995 kb on - strand at 526.042 kb on + strand at 526.084 kb on - strand at 526.116 kb on - strand, within RR41_RS02595 at 526.141 kb on - strand, within RR41_RS02595 at 526.141 kb on - strand, within RR41_RS02595 at 526.154 kb on + strand, within RR41_RS02595 at 526.161 kb on - strand, within RR41_RS02595 at 526.188 kb on - strand, within RR41_RS02595 at 526.189 kb on + strand, within RR41_RS02595 at 526.190 kb on - strand, within RR41_RS02595 at 526.199 kb on + strand, within RR41_RS02595 at 526.200 kb on - strand, within RR41_RS02595 at 526.230 kb on + strand, within RR41_RS02595 at 526.230 kb on + strand, within RR41_RS02595 at 526.230 kb on + strand, within RR41_RS02595 at 526.231 kb on - strand, within RR41_RS02595 at 526.231 kb on - strand, within RR41_RS02595 at 526.231 kb on - strand, within RR41_RS02595 at 526.242 kb on - strand, within RR41_RS02595 at 526.248 kb on - strand, within RR41_RS02595 at 526.253 kb on + strand, within RR41_RS02595 at 526.254 kb on - strand, within RR41_RS02595 at 526.256 kb on - strand, within RR41_RS02595 at 526.272 kb on - strand, within RR41_RS02595 at 526.292 kb on + strand, within RR41_RS02595 at 526.292 kb on + strand, within RR41_RS02595 at 526.292 kb on + strand, within RR41_RS02595 at 526.292 kb on + strand, within RR41_RS02595 at 526.292 kb on + strand, within RR41_RS02595 at 526.292 kb on + strand, within RR41_RS02595 at 526.293 kb on - strand, within RR41_RS02595 at 526.311 kb on - strand, within RR41_RS02595 at 526.333 kb on + strand, within RR41_RS02595 at 526.334 kb on - strand, within RR41_RS02595 at 526.334 kb on - strand, within RR41_RS02595 at 526.334 kb on - strand, within RR41_RS02595 at 526.334 kb on - strand, within RR41_RS02595 at 526.338 kb on - strand, within RR41_RS02595 at 526.338 kb on - strand, within RR41_RS02595 at 526.356 kb on + strand, within RR41_RS02595 at 526.362 kb on + strand, within RR41_RS02595 at 526.363 kb on - strand, within RR41_RS02595 at 526.363 kb on - strand, within RR41_RS02595 at 526.374 kb on - strand, within RR41_RS02595 at 526.374 kb on - strand, within RR41_RS02595 at 526.374 kb on - strand, within RR41_RS02595 at 526.415 kb on + strand, within RR41_RS02595 at 526.416 kb on - strand, within RR41_RS02595 at 526.416 kb on - strand, within RR41_RS02595 at 526.433 kb on + strand, within RR41_RS02595 at 526.440 kb on - strand at 526.440 kb on - strand at 526.444 kb on - strand at 526.444 kb on - strand at 526.522 kb on - strand at 526.527 kb on + strand at 526.532 kb on - strand at 526.532 kb on - strand at 526.554 kb on + strand at 526.555 kb on - strand at 526.555 kb on - strand at 526.566 kb on + strand at 526.611 kb on - strand at 526.616 kb on + strand at 526.617 kb on - strand at 526.620 kb on + strand at 526.620 kb on + strand at 526.662 kb on + strand, within RR41_RS02600 at 526.716 kb on + strand, within RR41_RS02600 at 526.717 kb on - strand, within RR41_RS02600 at 526.722 kb on + strand, within RR41_RS02600 at 526.747 kb on - strand, within RR41_RS02600 at 526.747 kb on - strand, within RR41_RS02600 at 526.749 kb on - strand, within RR41_RS02600 at 526.749 kb on - strand, within RR41_RS02600 at 526.749 kb on - strand, within RR41_RS02600 at 526.754 kb on + strand, within RR41_RS02600 at 526.758 kb on + strand, within RR41_RS02600 at 526.800 kb on + strand, within RR41_RS02600 at 526.816 kb on - strand, within RR41_RS02600 at 526.816 kb on - strand, within RR41_RS02600 at 526.833 kb on + strand, within RR41_RS02600 at 526.833 kb on + strand, within RR41_RS02600 at 526.833 kb on + strand, within RR41_RS02600 at 526.833 kb on + strand, within RR41_RS02600 at 526.833 kb on + strand, within RR41_RS02600 at 526.833 kb on + strand, within RR41_RS02600 at 526.836 kb on + strand, within RR41_RS02600 at 526.837 kb on - strand, within RR41_RS02600 at 526.837 kb on - strand, within RR41_RS02600 at 526.837 kb on - strand, within RR41_RS02600 at 526.910 kb on + strand, within RR41_RS02600 at 526.910 kb on + strand, within RR41_RS02600 at 526.910 kb on + strand, within RR41_RS02600 at 526.911 kb on - strand, within RR41_RS02600 at 526.914 kb on + strand, within RR41_RS02600 at 526.914 kb on + strand, within RR41_RS02600 at 526.914 kb on + strand, within RR41_RS02600 at 526.914 kb on + strand, within RR41_RS02600 at 526.914 kb on + strand, within RR41_RS02600 at 526.915 kb on - strand, within RR41_RS02600 at 526.915 kb on - strand, within RR41_RS02600 at 526.915 kb on - strand, within RR41_RS02600 at 526.915 kb on - strand, within RR41_RS02600 at 526.915 kb on - strand, within RR41_RS02600 at 526.918 kb on - strand, within RR41_RS02600 at 526.921 kb on + strand, within RR41_RS02600 at 526.939 kb on - strand, within RR41_RS02600 at 526.953 kb on - strand, within RR41_RS02600 at 526.969 kb on - strand, within RR41_RS02600 at 526.995 kb on + strand, within RR41_RS02600
Per-strain Table
Position Strand Gene LocusTag Fraction RPMI_TSB_serum temperature; 30C remove 524,347 + -3.2 524,347 + -2.0 524,347 + -0.0 524,347 + -3.0 524,348 - -2.0 524,348 - -2.0 524,352 - -2.8 524,422 - -0.0 524,505 + RR41_RS02585 0.19 -1.6 524,575 - RR41_RS02585 0.28 -2.0 524,620 + RR41_RS02585 0.33 -1.0 524,625 + RR41_RS02585 0.34 +1.6 524,665 - RR41_RS02585 0.39 -0.0 524,801 - RR41_RS02585 0.56 -1.6 524,801 - RR41_RS02585 0.56 -2.8 524,804 - RR41_RS02585 0.56 -1.6 524,839 - RR41_RS02585 0.60 -1.8 524,856 - RR41_RS02585 0.62 -2.0 524,902 + RR41_RS02585 0.68 -0.0 525,159 - -0.0 525,174 - -1.0 525,174 - -1.0 525,175 + -0.0 525,179 + +0.4 525,180 - -0.0 525,183 + -1.3 525,183 + +0.5 525,183 + -1.0 525,184 - -0.8 525,184 - -1.0 525,334 - RR41_RS02590 0.21 -2.0 525,442 + RR41_RS02590 0.33 -0.6 525,442 + RR41_RS02590 0.33 +0.8 525,501 + RR41_RS02590 0.40 -2.6 525,501 + RR41_RS02590 0.40 -1.6 525,502 - RR41_RS02590 0.41 -1.6 525,523 - RR41_RS02590 0.43 -2.0 525,540 + RR41_RS02590 0.45 -1.2 525,540 + RR41_RS02590 0.45 +2.0 525,615 + RR41_RS02590 0.54 -3.1 525,624 + RR41_RS02590 0.55 +0.6 525,688 + RR41_RS02590 0.63 +0.4 525,735 + RR41_RS02590 0.68 +1.7 525,740 - RR41_RS02590 0.69 -1.3 525,829 + RR41_RS02590 0.80 -3.3 525,830 - RR41_RS02590 0.80 -1.0 525,830 - RR41_RS02590 0.80 -2.0 525,884 - RR41_RS02590 0.86 -2.8 525,889 + RR41_RS02590 0.87 +0.2 525,889 + RR41_RS02590 0.87 -1.0 525,942 + -2.0 525,994 + -0.0 525,994 + +0.3 525,995 - -2.8 525,995 - -4.4 525,995 - +0.3 526,042 + -1.6 526,084 - +1.0 526,116 - RR41_RS02595 0.17 -0.0 526,141 - RR41_RS02595 0.23 -1.0 526,141 - RR41_RS02595 0.23 -0.6 526,154 + RR41_RS02595 0.25 -1.6 526,161 - RR41_RS02595 0.27 +1.4 526,188 - RR41_RS02595 0.33 -2.8 526,189 + RR41_RS02595 0.33 -1.6 526,190 - RR41_RS02595 0.34 -1.0 526,199 + RR41_RS02595 0.36 -0.0 526,200 - RR41_RS02595 0.36 +2.3 526,230 + RR41_RS02595 0.43 -1.0 526,230 + RR41_RS02595 0.43 -2.0 526,230 + RR41_RS02595 0.43 +0.5 526,231 - RR41_RS02595 0.43 -1.0 526,231 - RR41_RS02595 0.43 +1.0 526,231 - RR41_RS02595 0.43 +1.1 526,242 - RR41_RS02595 0.45 -1.6 526,248 - RR41_RS02595 0.47 +2.0 526,253 + RR41_RS02595 0.48 +1.6 526,254 - RR41_RS02595 0.48 -1.3 526,256 - RR41_RS02595 0.48 +0.8 526,272 - RR41_RS02595 0.52 -1.0 526,292 + RR41_RS02595 0.57 -0.0 526,292 + RR41_RS02595 0.57 +3.0 526,292 + RR41_RS02595 0.57 -2.3 526,292 + RR41_RS02595 0.57 -2.8 526,292 + RR41_RS02595 0.57 -0.0 526,292 + RR41_RS02595 0.57 -1.2 526,293 - RR41_RS02595 0.57 -3.2 526,311 - RR41_RS02595 0.61 -2.3 526,333 + RR41_RS02595 0.66 +0.4 526,334 - RR41_RS02595 0.66 -0.0 526,334 - RR41_RS02595 0.66 +0.1 526,334 - RR41_RS02595 0.66 +1.0 526,334 - RR41_RS02595 0.66 -0.0 526,338 - RR41_RS02595 0.67 -2.8 526,338 - RR41_RS02595 0.67 -1.6 526,356 + RR41_RS02595 0.71 -0.7 526,362 + RR41_RS02595 0.72 -0.0 526,363 - RR41_RS02595 0.73 +0.3 526,363 - RR41_RS02595 0.73 -0.6 526,374 - RR41_RS02595 0.75 +1.0 526,374 - RR41_RS02595 0.75 -2.6 526,374 - RR41_RS02595 0.75 -0.0 526,415 + RR41_RS02595 0.84 -0.0 526,416 - RR41_RS02595 0.84 -3.0 526,416 - RR41_RS02595 0.84 -1.0 526,433 + RR41_RS02595 0.88 -1.0 526,440 - +2.3 526,440 - -2.4 526,444 - -1.0 526,444 - -0.0 526,522 - -2.6 526,527 + +1.0 526,532 - +0.3 526,532 - -2.0 526,554 + -0.8 526,555 - -1.3 526,555 - -1.6 526,566 + -3.0 526,611 - -3.0 526,616 + +1.2 526,617 - -0.3 526,620 + -1.6 526,620 + -1.6 526,662 + RR41_RS02600 0.14 +2.0 526,716 + RR41_RS02600 0.22 -0.0 526,717 - RR41_RS02600 0.22 +1.3 526,722 + RR41_RS02600 0.23 +0.8 526,747 - RR41_RS02600 0.26 -2.3 526,747 - RR41_RS02600 0.26 +0.6 526,749 - RR41_RS02600 0.27 -0.0 526,749 - RR41_RS02600 0.27 -2.8 526,749 - RR41_RS02600 0.27 +0.3 526,754 + RR41_RS02600 0.27 -1.0 526,758 + RR41_RS02600 0.28 +1.3 526,800 + RR41_RS02600 0.34 +0.7 526,816 - RR41_RS02600 0.37 -2.0 526,816 - RR41_RS02600 0.37 +4.0 526,833 + RR41_RS02600 0.39 -2.6 526,833 + RR41_RS02600 0.39 +2.0 526,833 + RR41_RS02600 0.39 +0.6 526,833 + RR41_RS02600 0.39 +2.6 526,833 + RR41_RS02600 0.39 -0.0 526,833 + RR41_RS02600 0.39 -0.4 526,836 + RR41_RS02600 0.40 -0.4 526,837 - RR41_RS02600 0.40 +0.8 526,837 - RR41_RS02600 0.40 -0.7 526,837 - RR41_RS02600 0.40 -1.6 526,910 + RR41_RS02600 0.51 -0.6 526,910 + RR41_RS02600 0.51 +0.6 526,910 + RR41_RS02600 0.51 -0.0 526,911 - RR41_RS02600 0.51 +1.0 526,914 + RR41_RS02600 0.51 -2.0 526,914 + RR41_RS02600 0.51 -2.0 526,914 + RR41_RS02600 0.51 -0.0 526,914 + RR41_RS02600 0.51 -0.0 526,914 + RR41_RS02600 0.51 +1.2 526,915 - RR41_RS02600 0.51 +2.0 526,915 - RR41_RS02600 0.51 +2.0 526,915 - RR41_RS02600 0.51 -3.2 526,915 - RR41_RS02600 0.51 -1.6 526,915 - RR41_RS02600 0.51 -0.9 526,918 - RR41_RS02600 0.52 -3.3 526,921 + RR41_RS02600 0.52 -1.6 526,939 - RR41_RS02600 0.55 +0.2 526,953 - RR41_RS02600 0.57 -0.9 526,969 - RR41_RS02600 0.59 -3.2 526,995 + RR41_RS02600 0.63 +1.3
Or see this region's nucleotide sequence