Experiment: RPMI_TSB_serum temperature; 30C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR41_RS01505 and RR41_RS01510 are separated by 222 nucleotides RR41_RS01510 and RR41_RS01515 overlap by 1 nucleotides RR41_RS01515 and RR41_RS01520 are separated by 11 nucleotides
RR41_RS01505: RR41_RS01505 - sulfate ABC transporter substrate-binding protein, at 316,557 to 317,558
_RS01505
RR41_RS01510: RR41_RS01510 - 16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH, at 317,781 to 318,704
_RS01510
RR41_RS01515: RR41_RS01515 - cell division protein FtsL, at 318,704 to 319,036
_RS01515
RR41_RS01520: RR41_RS01520 - penicillin-binding protein PBP3, at 319,048 to 320,880
_RS01520
Position (kb) 317
318
319 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 316.883 kb on + strand, within RR41_RS01505 at 316.883 kb on + strand, within RR41_RS01505 at 316.896 kb on + strand, within RR41_RS01505 at 316.896 kb on + strand, within RR41_RS01505 at 316.896 kb on + strand, within RR41_RS01505 at 316.897 kb on - strand, within RR41_RS01505 at 316.899 kb on - strand, within RR41_RS01505 at 316.904 kb on + strand, within RR41_RS01505 at 316.918 kb on + strand, within RR41_RS01505 at 316.919 kb on - strand, within RR41_RS01505 at 316.964 kb on - strand, within RR41_RS01505 at 316.968 kb on - strand, within RR41_RS01505 at 316.968 kb on - strand, within RR41_RS01505 at 316.989 kb on - strand, within RR41_RS01505 at 317.029 kb on - strand, within RR41_RS01505 at 317.029 kb on - strand, within RR41_RS01505 at 317.029 kb on - strand, within RR41_RS01505 at 317.029 kb on - strand, within RR41_RS01505 at 317.046 kb on + strand, within RR41_RS01505 at 317.046 kb on + strand, within RR41_RS01505 at 317.047 kb on - strand, within RR41_RS01505 at 317.047 kb on - strand, within RR41_RS01505 at 317.047 kb on - strand, within RR41_RS01505 at 317.078 kb on + strand, within RR41_RS01505 at 317.079 kb on - strand, within RR41_RS01505 at 317.079 kb on - strand, within RR41_RS01505 at 317.150 kb on + strand, within RR41_RS01505 at 317.150 kb on + strand, within RR41_RS01505 at 317.150 kb on + strand, within RR41_RS01505 at 317.153 kb on + strand, within RR41_RS01505 at 317.153 kb on + strand, within RR41_RS01505 at 317.153 kb on + strand, within RR41_RS01505 at 317.154 kb on - strand, within RR41_RS01505 at 317.154 kb on - strand, within RR41_RS01505 at 317.154 kb on - strand, within RR41_RS01505 at 317.154 kb on - strand, within RR41_RS01505 at 317.154 kb on - strand, within RR41_RS01505 at 317.185 kb on + strand, within RR41_RS01505 at 317.185 kb on + strand, within RR41_RS01505 at 317.185 kb on + strand, within RR41_RS01505 at 317.209 kb on + strand, within RR41_RS01505 at 317.210 kb on - strand, within RR41_RS01505 at 317.237 kb on + strand, within RR41_RS01505 at 317.237 kb on + strand, within RR41_RS01505 at 317.237 kb on + strand, within RR41_RS01505 at 317.238 kb on - strand, within RR41_RS01505 at 317.238 kb on - strand, within RR41_RS01505 at 317.241 kb on + strand, within RR41_RS01505 at 317.241 kb on + strand, within RR41_RS01505 at 317.356 kb on + strand, within RR41_RS01505 at 317.368 kb on + strand, within RR41_RS01505 at 317.395 kb on - strand, within RR41_RS01505 at 317.430 kb on + strand, within RR41_RS01505 at 317.431 kb on - strand, within RR41_RS01505 at 317.431 kb on - strand, within RR41_RS01505 at 317.431 kb on - strand, within RR41_RS01505 at 317.459 kb on - strand at 317.541 kb on + strand at 317.541 kb on + strand at 317.541 kb on + strand at 317.542 kb on - strand at 317.542 kb on - strand at 317.577 kb on - strand at 317.604 kb on + strand at 317.607 kb on - strand at 317.697 kb on - strand at 317.761 kb on + strand at 317.850 kb on + strand at 317.923 kb on + strand, within RR41_RS01510 at 317.925 kb on + strand, within RR41_RS01510 at 318.079 kb on + strand, within RR41_RS01510 at 318.079 kb on + strand, within RR41_RS01510 at 318.193 kb on + strand, within RR41_RS01510 at 318.419 kb on + strand, within RR41_RS01510 at 318.541 kb on + strand, within RR41_RS01510
Per-strain Table
Position Strand Gene LocusTag Fraction RPMI_TSB_serum temperature; 30C remove 316,883 + RR41_RS01505 0.33 -1.1 316,883 + RR41_RS01505 0.33 -3.3 316,896 + RR41_RS01505 0.34 -3.3 316,896 + RR41_RS01505 0.34 -2.1 316,896 + RR41_RS01505 0.34 -2.4 316,897 - RR41_RS01505 0.34 -0.1 316,899 - RR41_RS01505 0.34 -0.1 316,904 + RR41_RS01505 0.35 +0.6 316,918 + RR41_RS01505 0.36 -1.8 316,919 - RR41_RS01505 0.36 -1.7 316,964 - RR41_RS01505 0.41 -0.1 316,968 - RR41_RS01505 0.41 +0.9 316,968 - RR41_RS01505 0.41 -1.1 316,989 - RR41_RS01505 0.43 +3.2 317,029 - RR41_RS01505 0.47 -1.6 317,029 - RR41_RS01505 0.47 +0.4 317,029 - RR41_RS01505 0.47 +0.3 317,029 - RR41_RS01505 0.47 -0.8 317,046 + RR41_RS01505 0.49 +0.8 317,046 + RR41_RS01505 0.49 +1.0 317,047 - RR41_RS01505 0.49 -2.7 317,047 - RR41_RS01505 0.49 -1.7 317,047 - RR41_RS01505 0.49 +1.5 317,078 + RR41_RS01505 0.52 -0.8 317,079 - RR41_RS01505 0.52 +2.2 317,079 - RR41_RS01505 0.52 +1.8 317,150 + RR41_RS01505 0.59 -1.1 317,150 + RR41_RS01505 0.59 -2.1 317,150 + RR41_RS01505 0.59 -1.9 317,153 + RR41_RS01505 0.59 -1.7 317,153 + RR41_RS01505 0.59 -1.7 317,153 + RR41_RS01505 0.59 -1.8 317,154 - RR41_RS01505 0.60 -1.7 317,154 - RR41_RS01505 0.60 -0.4 317,154 - RR41_RS01505 0.60 +1.1 317,154 - RR41_RS01505 0.60 +0.8 317,154 - RR41_RS01505 0.60 -0.1 317,185 + RR41_RS01505 0.63 -0.1 317,185 + RR41_RS01505 0.63 -0.3 317,185 + RR41_RS01505 0.63 -2.3 317,209 + RR41_RS01505 0.65 -0.4 317,210 - RR41_RS01505 0.65 +1.8 317,237 + RR41_RS01505 0.68 +1.0 317,237 + RR41_RS01505 0.68 -0.1 317,237 + RR41_RS01505 0.68 +1.2 317,238 - RR41_RS01505 0.68 -0.7 317,238 - RR41_RS01505 0.68 +0.9 317,241 + RR41_RS01505 0.68 -1.7 317,241 + RR41_RS01505 0.68 +2.7 317,356 + RR41_RS01505 0.80 +0.6 317,368 + RR41_RS01505 0.81 -1.1 317,395 - RR41_RS01505 0.84 -3.0 317,430 + RR41_RS01505 0.87 -1.1 317,431 - RR41_RS01505 0.87 -0.7 317,431 - RR41_RS01505 0.87 +0.4 317,431 - RR41_RS01505 0.87 -2.7 317,459 - -0.1 317,541 + -1.4 317,541 + -0.8 317,541 + -0.5 317,542 - -1.6 317,542 - +0.9 317,577 - -0.1 317,604 + +0.0 317,607 - +1.6 317,697 - -3.1 317,761 + -2.1 317,850 + -1.1 317,923 + RR41_RS01510 0.15 -1.7 317,925 + RR41_RS01510 0.16 -1.7 318,079 + RR41_RS01510 0.32 +0.9 318,079 + RR41_RS01510 0.32 -0.7 318,193 + RR41_RS01510 0.45 -1.4 318,419 + RR41_RS01510 0.69 -2.6 318,541 + RR41_RS01510 0.82 -1.7
Or see this region's nucleotide sequence