Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GEMAOFIL_02026 and GEMAOFIL_02027 are separated by 345 nucleotides GEMAOFIL_02027 and GEMAOFIL_02028 are separated by 96 nucleotides GEMAOFIL_02028 and GEMAOFIL_02029 overlap by 4 nucleotides GEMAOFIL_02029 and GEMAOFIL_02030 overlap by 4 nucleotides
GEMAOFIL_02026: GEMAOFIL_02026 - FKBP-type 22 kDa peptidyl-prolyl cis-trans isomerase, at 2,228,832 to 2,229,563
_02026
GEMAOFIL_02027: GEMAOFIL_02027 - hypothetical protein, at 2,229,909 to 2,230,364
_02027
GEMAOFIL_02028: GEMAOFIL_02028 - hypothetical protein, at 2,230,461 to 2,230,799
_02028
GEMAOFIL_02029: GEMAOFIL_02029 - hypothetical protein, at 2,230,796 to 2,231,167
_02029
GEMAOFIL_02030: GEMAOFIL_02030 - hypothetical protein, at 2,231,164 to 2,232,930
_02030
Position (kb)
2229
2230
2231 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2228.909 kb on - strand, within GEMAOFIL_02026 at 2228.909 kb on - strand, within GEMAOFIL_02026 at 2229.010 kb on + strand, within GEMAOFIL_02026 at 2229.213 kb on + strand, within GEMAOFIL_02026 at 2229.276 kb on + strand, within GEMAOFIL_02026 at 2229.276 kb on + strand, within GEMAOFIL_02026 at 2229.277 kb on - strand, within GEMAOFIL_02026 at 2229.277 kb on - strand, within GEMAOFIL_02026 at 2229.417 kb on + strand, within GEMAOFIL_02026 at 2229.418 kb on - strand, within GEMAOFIL_02026 at 2229.418 kb on - strand, within GEMAOFIL_02026 at 2229.418 kb on - strand, within GEMAOFIL_02026 at 2229.439 kb on - strand, within GEMAOFIL_02026 at 2229.630 kb on + strand at 2229.631 kb on - strand at 2229.631 kb on - strand at 2229.635 kb on + strand at 2229.636 kb on - strand at 2229.700 kb on + strand at 2229.701 kb on - strand at 2229.710 kb on + strand at 2229.710 kb on + strand at 2229.710 kb on + strand at 2229.711 kb on - strand at 2229.712 kb on + strand at 2229.712 kb on + strand at 2229.712 kb on + strand at 2229.713 kb on - strand at 2229.812 kb on + strand at 2229.813 kb on - strand at 2230.080 kb on + strand, within GEMAOFIL_02027 at 2230.113 kb on - strand, within GEMAOFIL_02027 at 2230.197 kb on - strand, within GEMAOFIL_02027 at 2230.197 kb on - strand, within GEMAOFIL_02027 at 2230.401 kb on - strand at 2230.407 kb on - strand at 2230.415 kb on - strand at 2230.427 kb on + strand at 2230.428 kb on - strand at 2230.428 kb on - strand at 2230.428 kb on - strand at 2230.434 kb on + strand at 2230.434 kb on + strand at 2230.435 kb on - strand at 2230.444 kb on + strand at 2230.449 kb on + strand at 2230.466 kb on + strand at 2230.466 kb on + strand at 2230.537 kb on + strand, within GEMAOFIL_02028 at 2230.562 kb on + strand, within GEMAOFIL_02028 at 2230.562 kb on + strand, within GEMAOFIL_02028 at 2230.563 kb on - strand, within GEMAOFIL_02028 at 2230.581 kb on - strand, within GEMAOFIL_02028 at 2230.581 kb on - strand, within GEMAOFIL_02028 at 2230.698 kb on + strand, within GEMAOFIL_02028 at 2230.699 kb on - strand, within GEMAOFIL_02028 at 2230.803 kb on + strand at 2230.804 kb on - strand at 2230.804 kb on - strand at 2230.807 kb on + strand at 2230.808 kb on - strand at 2230.808 kb on - strand at 2230.808 kb on - strand at 2230.881 kb on - strand, within GEMAOFIL_02029 at 2231.218 kb on + strand at 2231.297 kb on + strand at 2231.299 kb on + strand at 2231.299 kb on + strand at 2231.299 kb on + strand at 2231.300 kb on - strand at 2231.300 kb on - strand at 2231.300 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 2,228,909 - GEMAOFIL_02026 0.11 +1.0 2,228,909 - GEMAOFIL_02026 0.11 +1.0 2,229,010 + GEMAOFIL_02026 0.24 +0.9 2,229,213 + GEMAOFIL_02026 0.52 +0.5 2,229,276 + GEMAOFIL_02026 0.61 +0.4 2,229,276 + GEMAOFIL_02026 0.61 +0.8 2,229,277 - GEMAOFIL_02026 0.61 +0.1 2,229,277 - GEMAOFIL_02026 0.61 +0.9 2,229,417 + GEMAOFIL_02026 0.80 +0.3 2,229,418 - GEMAOFIL_02026 0.80 -0.2 2,229,418 - GEMAOFIL_02026 0.80 -0.4 2,229,418 - GEMAOFIL_02026 0.80 +0.0 2,229,439 - GEMAOFIL_02026 0.83 +0.5 2,229,630 + -0.7 2,229,631 - +0.5 2,229,631 - -0.0 2,229,635 + +0.9 2,229,636 - -1.0 2,229,700 + +0.4 2,229,701 - -0.1 2,229,710 + +1.2 2,229,710 + +0.4 2,229,710 + +0.7 2,229,711 - +0.1 2,229,712 + -0.4 2,229,712 + +0.4 2,229,712 + +0.1 2,229,713 - -0.9 2,229,812 + +0.0 2,229,813 - -2.1 2,230,080 + GEMAOFIL_02027 0.38 +0.3 2,230,113 - GEMAOFIL_02027 0.45 -0.1 2,230,197 - GEMAOFIL_02027 0.63 -0.7 2,230,197 - GEMAOFIL_02027 0.63 +0.3 2,230,401 - -0.1 2,230,407 - +0.1 2,230,415 - -0.1 2,230,427 + -4.2 2,230,428 - +0.0 2,230,428 - -1.0 2,230,428 - -2.2 2,230,434 + +1.5 2,230,434 + +0.6 2,230,435 - -0.4 2,230,444 + +0.2 2,230,449 + +0.4 2,230,466 + +0.1 2,230,466 + -0.1 2,230,537 + GEMAOFIL_02028 0.22 -0.5 2,230,562 + GEMAOFIL_02028 0.30 -0.4 2,230,562 + GEMAOFIL_02028 0.30 -0.3 2,230,563 - GEMAOFIL_02028 0.30 +1.5 2,230,581 - GEMAOFIL_02028 0.35 -0.2 2,230,581 - GEMAOFIL_02028 0.35 +0.5 2,230,698 + GEMAOFIL_02028 0.70 +0.2 2,230,699 - GEMAOFIL_02028 0.70 -0.2 2,230,803 + +0.1 2,230,804 - -0.1 2,230,804 - -0.6 2,230,807 + -0.5 2,230,808 - +0.7 2,230,808 - -2.2 2,230,808 - +1.9 2,230,881 - GEMAOFIL_02029 0.23 +0.6 2,231,218 + +0.3 2,231,297 + -0.7 2,231,299 + -0.1 2,231,299 + +0.1 2,231,299 + +0.5 2,231,300 - -0.2 2,231,300 - +1.2 2,231,300 - +0.3
Or see this region's nucleotide sequence