Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GEMAOFIL_03011 and GEMAOFIL_03012 are separated by 587 nucleotides GEMAOFIL_03012 and GEMAOFIL_03013 overlap by 4 nucleotides GEMAOFIL_03013 and GEMAOFIL_03014 are separated by 483 nucleotides
GEMAOFIL_03011: GEMAOFIL_03011 - Putrescine importer PuuP, at 3,303,560 to 3,304,984
_03011
GEMAOFIL_03012: GEMAOFIL_03012 - 2-amino-5-chloromuconic acid deaminase, at 3,305,572 to 3,306,672
_03012
GEMAOFIL_03013: GEMAOFIL_03013 - hypothetical protein, at 3,306,669 to 3,307,175
_03013
GEMAOFIL_03014: GEMAOFIL_03014 - N-((2S)-2-amino-2-carboxyethyl)-L-glutamate dehydrogenase, at 3,307,659 to 3,308,798
_03014
Position (kb)
3305
3306
3307 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3304.577 kb on - strand, within GEMAOFIL_03011 at 3304.597 kb on + strand, within GEMAOFIL_03011 at 3304.597 kb on + strand, within GEMAOFIL_03011 at 3304.597 kb on + strand, within GEMAOFIL_03011 at 3304.634 kb on - strand, within GEMAOFIL_03011 at 3304.648 kb on - strand, within GEMAOFIL_03011 at 3304.655 kb on + strand, within GEMAOFIL_03011 at 3304.683 kb on - strand, within GEMAOFIL_03011 at 3304.710 kb on + strand, within GEMAOFIL_03011 at 3304.711 kb on - strand, within GEMAOFIL_03011 at 3304.716 kb on + strand, within GEMAOFIL_03011 at 3304.763 kb on + strand, within GEMAOFIL_03011 at 3304.764 kb on - strand, within GEMAOFIL_03011 at 3304.825 kb on + strand, within GEMAOFIL_03011 at 3304.896 kb on - strand at 3304.916 kb on + strand at 3304.921 kb on + strand at 3304.928 kb on + strand at 3305.005 kb on - strand at 3305.005 kb on - strand at 3305.024 kb on + strand at 3305.083 kb on - strand at 3305.124 kb on + strand at 3305.124 kb on + strand at 3305.125 kb on - strand at 3305.163 kb on + strand at 3305.163 kb on + strand at 3305.235 kb on + strand at 3305.236 kb on - strand at 3305.264 kb on + strand at 3305.306 kb on - strand at 3305.313 kb on + strand at 3305.313 kb on + strand at 3305.392 kb on + strand at 3305.392 kb on + strand at 3305.392 kb on + strand at 3305.393 kb on - strand at 3305.393 kb on - strand at 3305.570 kb on - strand at 3305.752 kb on + strand, within GEMAOFIL_03012 at 3305.752 kb on + strand, within GEMAOFIL_03012 at 3305.752 kb on + strand, within GEMAOFIL_03012 at 3305.752 kb on + strand, within GEMAOFIL_03012 at 3305.752 kb on + strand, within GEMAOFIL_03012 at 3305.752 kb on + strand, within GEMAOFIL_03012 at 3305.752 kb on + strand, within GEMAOFIL_03012 at 3305.752 kb on + strand, within GEMAOFIL_03012 at 3305.752 kb on + strand, within GEMAOFIL_03012 at 3305.753 kb on - strand, within GEMAOFIL_03012 at 3305.753 kb on - strand, within GEMAOFIL_03012 at 3305.753 kb on - strand, within GEMAOFIL_03012 at 3305.753 kb on - strand, within GEMAOFIL_03012 at 3305.753 kb on - strand, within GEMAOFIL_03012 at 3305.753 kb on - strand, within GEMAOFIL_03012 at 3305.819 kb on - strand, within GEMAOFIL_03012 at 3305.819 kb on - strand, within GEMAOFIL_03012 at 3305.869 kb on + strand, within GEMAOFIL_03012 at 3305.902 kb on + strand, within GEMAOFIL_03012 at 3305.930 kb on - strand, within GEMAOFIL_03012 at 3305.945 kb on + strand, within GEMAOFIL_03012 at 3305.946 kb on - strand, within GEMAOFIL_03012 at 3305.946 kb on - strand, within GEMAOFIL_03012 at 3305.946 kb on - strand, within GEMAOFIL_03012 at 3305.946 kb on - strand, within GEMAOFIL_03012 at 3305.954 kb on + strand, within GEMAOFIL_03012 at 3305.954 kb on + strand, within GEMAOFIL_03012 at 3305.955 kb on - strand, within GEMAOFIL_03012 at 3305.976 kb on + strand, within GEMAOFIL_03012 at 3305.977 kb on - strand, within GEMAOFIL_03012 at 3306.106 kb on + strand, within GEMAOFIL_03012 at 3306.106 kb on + strand, within GEMAOFIL_03012 at 3306.113 kb on - strand, within GEMAOFIL_03012 at 3306.135 kb on + strand, within GEMAOFIL_03012 at 3306.135 kb on + strand, within GEMAOFIL_03012 at 3306.330 kb on + strand, within GEMAOFIL_03012 at 3306.421 kb on - strand, within GEMAOFIL_03012 at 3306.868 kb on - strand, within GEMAOFIL_03013 at 3306.868 kb on - strand, within GEMAOFIL_03013 at 3306.868 kb on - strand, within GEMAOFIL_03013 at 3306.893 kb on + strand, within GEMAOFIL_03013 at 3306.894 kb on - strand, within GEMAOFIL_03013 at 3306.985 kb on + strand, within GEMAOFIL_03013 at 3307.083 kb on - strand, within GEMAOFIL_03013 at 3307.084 kb on + strand, within GEMAOFIL_03013 at 3307.085 kb on - strand, within GEMAOFIL_03013 at 3307.226 kb on + strand at 3307.227 kb on - strand at 3307.305 kb on + strand at 3307.312 kb on + strand at 3307.312 kb on + strand at 3307.313 kb on - strand at 3307.410 kb on + strand at 3307.411 kb on - strand at 3307.414 kb on + strand at 3307.472 kb on + strand at 3307.634 kb on + strand at 3307.670 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 3,304,577 - GEMAOFIL_03011 0.71 +1.0 3,304,597 + GEMAOFIL_03011 0.73 -0.2 3,304,597 + GEMAOFIL_03011 0.73 -0.4 3,304,597 + GEMAOFIL_03011 0.73 -0.1 3,304,634 - GEMAOFIL_03011 0.75 +0.0 3,304,648 - GEMAOFIL_03011 0.76 -0.1 3,304,655 + GEMAOFIL_03011 0.77 -0.6 3,304,683 - GEMAOFIL_03011 0.79 +0.5 3,304,710 + GEMAOFIL_03011 0.81 +0.2 3,304,711 - GEMAOFIL_03011 0.81 +0.7 3,304,716 + GEMAOFIL_03011 0.81 -0.1 3,304,763 + GEMAOFIL_03011 0.84 +0.9 3,304,764 - GEMAOFIL_03011 0.84 +0.3 3,304,825 + GEMAOFIL_03011 0.89 -0.4 3,304,896 - +0.1 3,304,916 + -0.3 3,304,921 + -0.4 3,304,928 + +0.4 3,305,005 - +0.5 3,305,005 - +0.6 3,305,024 + -0.0 3,305,083 - -0.7 3,305,124 + +1.0 3,305,124 + +0.7 3,305,125 - -0.8 3,305,163 + -0.4 3,305,163 + -1.4 3,305,235 + +1.0 3,305,236 - -1.7 3,305,264 + -0.7 3,305,306 - +0.6 3,305,313 + +0.5 3,305,313 + -0.4 3,305,392 + +0.3 3,305,392 + +1.2 3,305,392 + +1.3 3,305,393 - +0.1 3,305,393 - +0.1 3,305,570 - -0.1 3,305,752 + GEMAOFIL_03012 0.16 +0.2 3,305,752 + GEMAOFIL_03012 0.16 +0.9 3,305,752 + GEMAOFIL_03012 0.16 -0.5 3,305,752 + GEMAOFIL_03012 0.16 -0.4 3,305,752 + GEMAOFIL_03012 0.16 +0.0 3,305,752 + GEMAOFIL_03012 0.16 -0.5 3,305,752 + GEMAOFIL_03012 0.16 +1.1 3,305,752 + GEMAOFIL_03012 0.16 -0.1 3,305,752 + GEMAOFIL_03012 0.16 -0.0 3,305,753 - GEMAOFIL_03012 0.16 +0.2 3,305,753 - GEMAOFIL_03012 0.16 -2.7 3,305,753 - GEMAOFIL_03012 0.16 -0.3 3,305,753 - GEMAOFIL_03012 0.16 +0.1 3,305,753 - GEMAOFIL_03012 0.16 -0.3 3,305,753 - GEMAOFIL_03012 0.16 -0.5 3,305,819 - GEMAOFIL_03012 0.22 +0.2 3,305,819 - GEMAOFIL_03012 0.22 -0.6 3,305,869 + GEMAOFIL_03012 0.27 +0.5 3,305,902 + GEMAOFIL_03012 0.30 +1.6 3,305,930 - GEMAOFIL_03012 0.33 -0.0 3,305,945 + GEMAOFIL_03012 0.34 +0.2 3,305,946 - GEMAOFIL_03012 0.34 -0.9 3,305,946 - GEMAOFIL_03012 0.34 +0.9 3,305,946 - GEMAOFIL_03012 0.34 +0.2 3,305,946 - GEMAOFIL_03012 0.34 -0.2 3,305,954 + GEMAOFIL_03012 0.35 -0.1 3,305,954 + GEMAOFIL_03012 0.35 -0.4 3,305,955 - GEMAOFIL_03012 0.35 -0.7 3,305,976 + GEMAOFIL_03012 0.37 -1.4 3,305,977 - GEMAOFIL_03012 0.37 +1.8 3,306,106 + GEMAOFIL_03012 0.49 -0.5 3,306,106 + GEMAOFIL_03012 0.49 -0.3 3,306,113 - GEMAOFIL_03012 0.49 -0.2 3,306,135 + GEMAOFIL_03012 0.51 +0.2 3,306,135 + GEMAOFIL_03012 0.51 -0.9 3,306,330 + GEMAOFIL_03012 0.69 -0.1 3,306,421 - GEMAOFIL_03012 0.77 +0.4 3,306,868 - GEMAOFIL_03013 0.39 +0.2 3,306,868 - GEMAOFIL_03013 0.39 +0.0 3,306,868 - GEMAOFIL_03013 0.39 +0.2 3,306,893 + GEMAOFIL_03013 0.44 -0.1 3,306,894 - GEMAOFIL_03013 0.44 +0.8 3,306,985 + GEMAOFIL_03013 0.62 +1.0 3,307,083 - GEMAOFIL_03013 0.82 -0.4 3,307,084 + GEMAOFIL_03013 0.82 +0.2 3,307,085 - GEMAOFIL_03013 0.82 -1.1 3,307,226 + +0.8 3,307,227 - +0.2 3,307,305 + -0.4 3,307,312 + -0.8 3,307,312 + -0.2 3,307,313 - +0.5 3,307,410 + -3.4 3,307,411 - +0.4 3,307,414 + -0.5 3,307,472 + +1.3 3,307,634 + -0.6 3,307,670 - +0.4
Or see this region's nucleotide sequence