Experiment: Methanol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF994 and GFF995 are separated by 118 nucleotides GFF995 and GFF996 are separated by 12 nucleotides
GFF994 - Acetyl-coenzyme A synthetase, at 230,071 to 232,047
GFF994
GFF995 - Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme, at 232,166 to 234,904
GFF995
GFF996 - hypothetical protein, at 234,917 to 236,887
GFF996
Position (kb) 232
233
234
235 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 231.198 kb on - strand, within GFF994 at 231.210 kb on + strand, within GFF994 at 231.210 kb on + strand, within GFF994 at 231.210 kb on + strand, within GFF994 at 231.210 kb on + strand, within GFF994 at 231.211 kb on - strand, within GFF994 at 231.211 kb on - strand, within GFF994 at 231.211 kb on - strand, within GFF994 at 231.211 kb on - strand, within GFF994 at 231.211 kb on - strand, within GFF994 at 231.211 kb on - strand, within GFF994 at 231.211 kb on - strand, within GFF994 at 231.217 kb on + strand, within GFF994 at 231.217 kb on + strand, within GFF994 at 231.217 kb on + strand, within GFF994 at 231.218 kb on - strand, within GFF994 at 231.251 kb on + strand, within GFF994 at 231.251 kb on + strand, within GFF994 at 231.252 kb on - strand, within GFF994 at 231.281 kb on - strand, within GFF994 at 231.342 kb on + strand, within GFF994 at 231.342 kb on + strand, within GFF994 at 231.342 kb on + strand, within GFF994 at 231.342 kb on + strand, within GFF994 at 231.342 kb on + strand, within GFF994 at 231.343 kb on - strand, within GFF994 at 231.483 kb on + strand, within GFF994 at 231.483 kb on + strand, within GFF994 at 231.484 kb on - strand, within GFF994 at 231.565 kb on - strand, within GFF994 at 231.623 kb on + strand, within GFF994 at 231.623 kb on + strand, within GFF994 at 231.623 kb on + strand, within GFF994 at 231.623 kb on + strand, within GFF994 at 231.623 kb on + strand, within GFF994 at 231.623 kb on + strand, within GFF994 at 231.624 kb on - strand, within GFF994 at 231.624 kb on - strand, within GFF994 at 231.624 kb on - strand, within GFF994 at 231.624 kb on - strand, within GFF994 at 231.624 kb on - strand, within GFF994 at 231.624 kb on - strand, within GFF994 at 231.624 kb on - strand, within GFF994 at 231.627 kb on + strand, within GFF994 at 231.627 kb on + strand, within GFF994 at 231.627 kb on + strand, within GFF994 at 231.628 kb on - strand, within GFF994 at 231.628 kb on - strand, within GFF994 at 231.628 kb on - strand, within GFF994 at 231.628 kb on - strand, within GFF994 at 231.628 kb on - strand, within GFF994 at 231.628 kb on - strand, within GFF994 at 231.628 kb on - strand, within GFF994 at 231.696 kb on - strand, within GFF994 at 231.707 kb on + strand, within GFF994 at 231.708 kb on - strand, within GFF994 at 231.708 kb on - strand, within GFF994 at 231.747 kb on + strand, within GFF994 at 231.748 kb on - strand, within GFF994 at 231.808 kb on + strand, within GFF994 at 231.809 kb on - strand, within GFF994 at 231.809 kb on - strand, within GFF994 at 231.809 kb on - strand, within GFF994 at 231.809 kb on - strand, within GFF994 at 231.833 kb on + strand, within GFF994 at 231.833 kb on + strand, within GFF994 at 231.833 kb on + strand, within GFF994 at 231.834 kb on - strand, within GFF994 at 231.834 kb on - strand, within GFF994 at 231.834 kb on - strand, within GFF994 at 231.868 kb on - strand at 231.868 kb on - strand at 231.886 kb on + strand at 231.887 kb on - strand at 231.889 kb on + strand at 231.890 kb on - strand at 231.890 kb on - strand at 231.923 kb on + strand at 231.923 kb on + strand at 231.924 kb on - strand at 231.956 kb on + strand at 231.956 kb on + strand at 231.956 kb on + strand at 231.957 kb on - strand at 231.957 kb on - strand at 232.013 kb on - strand at 232.030 kb on + strand at 232.030 kb on + strand at 232.030 kb on + strand at 232.041 kb on + strand at 232.047 kb on + strand at 232.047 kb on + strand at 232.047 kb on + strand at 232.047 kb on + strand at 232.047 kb on + strand at 232.048 kb on - strand at 232.048 kb on - strand at 232.048 kb on - strand at 232.048 kb on - strand at 232.104 kb on - strand at 232.106 kb on + strand at 232.106 kb on + strand at 232.106 kb on + strand at 232.106 kb on + strand at 232.106 kb on + strand at 232.106 kb on + strand at 232.106 kb on + strand at 232.107 kb on - strand at 232.107 kb on - strand at 232.107 kb on - strand at 232.107 kb on - strand at 232.150 kb on - strand at 232.152 kb on + strand at 232.152 kb on + strand at 232.153 kb on - strand at 232.153 kb on - strand at 232.312 kb on - strand at 232.313 kb on + strand at 232.324 kb on + strand at 232.353 kb on + strand at 232.527 kb on - strand, within GFF995 at 232.527 kb on - strand, within GFF995 at 232.547 kb on - strand, within GFF995 at 232.547 kb on - strand, within GFF995 at 232.547 kb on - strand, within GFF995 at 232.550 kb on + strand, within GFF995 at 232.550 kb on + strand, within GFF995 at 232.551 kb on - strand, within GFF995 at 232.551 kb on - strand, within GFF995 at 232.645 kb on + strand, within GFF995 at 232.647 kb on + strand, within GFF995 at 232.647 kb on + strand, within GFF995 at 232.647 kb on + strand, within GFF995 at 232.647 kb on + strand, within GFF995 at 232.647 kb on + strand, within GFF995 at 232.647 kb on + strand, within GFF995 at 232.648 kb on - strand, within GFF995 at 232.860 kb on - strand, within GFF995 at 232.868 kb on + strand, within GFF995 at 232.868 kb on + strand, within GFF995 at 232.900 kb on + strand, within GFF995 at 232.970 kb on + strand, within GFF995 at 232.970 kb on + strand, within GFF995 at 232.988 kb on + strand, within GFF995 at 232.988 kb on + strand, within GFF995 at 232.989 kb on - strand, within GFF995 at 232.989 kb on - strand, within GFF995 at 232.989 kb on - strand, within GFF995 at 232.989 kb on - strand, within GFF995 at 233.056 kb on + strand, within GFF995 at 233.056 kb on + strand, within GFF995 at 233.057 kb on - strand, within GFF995 at 233.231 kb on + strand, within GFF995 at 233.237 kb on + strand, within GFF995 at 233.258 kb on - strand, within GFF995 at 233.267 kb on + strand, within GFF995 at 233.312 kb on + strand, within GFF995 at 233.312 kb on + strand, within GFF995 at 233.363 kb on - strand, within GFF995 at 233.383 kb on + strand, within GFF995 at 233.384 kb on - strand, within GFF995 at 233.394 kb on + strand, within GFF995 at 233.394 kb on + strand, within GFF995 at 233.394 kb on + strand, within GFF995 at 233.442 kb on + strand, within GFF995 at 233.442 kb on + strand, within GFF995 at 233.516 kb on + strand, within GFF995 at 233.626 kb on - strand, within GFF995 at 233.723 kb on + strand, within GFF995 at 233.804 kb on + strand, within GFF995 at 233.898 kb on + strand, within GFF995 at 233.898 kb on + strand, within GFF995 at 234.167 kb on + strand, within GFF995 at 234.187 kb on + strand, within GFF995 at 234.256 kb on + strand, within GFF995 at 234.346 kb on + strand, within GFF995 at 234.368 kb on + strand, within GFF995 at 234.379 kb on + strand, within GFF995 at 234.616 kb on + strand, within GFF995 at 234.649 kb on + strand at 234.873 kb on + strand at 234.873 kb on + strand at 234.874 kb on - strand at 234.874 kb on - strand at 234.874 kb on - strand at 234.919 kb on - strand at 234.975 kb on + strand at 234.975 kb on + strand at 234.975 kb on + strand at 235.014 kb on + strand at 235.036 kb on + strand at 235.036 kb on + strand at 235.036 kb on + strand at 235.036 kb on + strand at 235.036 kb on + strand at 235.036 kb on + strand at 235.036 kb on + strand at 235.036 kb on + strand at 235.036 kb on + strand at 235.036 kb on + strand at 235.036 kb on + strand at 235.036 kb on + strand at 235.036 kb on + strand at 235.036 kb on + strand at 235.036 kb on + strand at 235.036 kb on + strand at 235.037 kb on - strand at 235.037 kb on - strand at 235.037 kb on - strand at 235.037 kb on - strand at 235.037 kb on - strand at 235.037 kb on - strand at 235.037 kb on - strand at 235.037 kb on - strand at 235.037 kb on - strand at 235.037 kb on - strand at 235.038 kb on + strand at 235.039 kb on - strand at 235.050 kb on + strand at 235.051 kb on - strand at 235.056 kb on + strand at 235.057 kb on - strand at 235.057 kb on - strand at 235.057 kb on - strand at 235.057 kb on - strand at 235.057 kb on - strand at 235.081 kb on + strand at 235.081 kb on + strand at 235.082 kb on - strand at 235.082 kb on - strand at 235.082 kb on - strand at 235.082 kb on - strand at 235.082 kb on - strand at 235.082 kb on - strand at 235.082 kb on - strand at 235.082 kb on - strand at 235.082 kb on - strand at 235.085 kb on + strand at 235.085 kb on + strand at 235.086 kb on - strand at 235.086 kb on - strand at 235.086 kb on - strand at 235.087 kb on + strand at 235.087 kb on + strand at 235.087 kb on + strand at 235.088 kb on - strand at 235.136 kb on + strand, within GFF996 at 235.136 kb on + strand, within GFF996 at 235.136 kb on + strand, within GFF996 at 235.137 kb on - strand, within GFF996 at 235.154 kb on - strand, within GFF996 at 235.235 kb on - strand, within GFF996 at 235.401 kb on - strand, within GFF996 at 235.420 kb on - strand, within GFF996 at 235.420 kb on - strand, within GFF996 at 235.457 kb on + strand, within GFF996 at 235.458 kb on - strand, within GFF996 at 235.458 kb on - strand, within GFF996 at 235.458 kb on - strand, within GFF996 at 235.517 kb on + strand, within GFF996 at 235.630 kb on + strand, within GFF996 at 235.643 kb on + strand, within GFF996 at 235.669 kb on - strand, within GFF996 at 235.702 kb on + strand, within GFF996 at 235.703 kb on - strand, within GFF996 at 235.703 kb on - strand, within GFF996 at 235.703 kb on - strand, within GFF996 at 235.703 kb on - strand, within GFF996 at 235.746 kb on + strand, within GFF996 at 235.746 kb on + strand, within GFF996 at 235.747 kb on - strand, within GFF996 at 235.763 kb on + strand, within GFF996 at 235.764 kb on - strand, within GFF996 at 235.764 kb on - strand, within GFF996 at 235.764 kb on - strand, within GFF996 at 235.764 kb on - strand, within GFF996 at 235.764 kb on - strand, within GFF996 at 235.779 kb on - strand, within GFF996 at 235.779 kb on - strand, within GFF996 at 235.779 kb on - strand, within GFF996 at 235.842 kb on + strand, within GFF996 at 235.874 kb on + strand, within GFF996 at 235.874 kb on + strand, within GFF996 at 235.875 kb on - strand, within GFF996 at 235.875 kb on - strand, within GFF996 at 235.875 kb on - strand, within GFF996 at 235.875 kb on - strand, within GFF996 at 235.875 kb on - strand, within GFF996 at 235.875 kb on - strand, within GFF996 at 235.875 kb on - strand, within GFF996
Per-strain Table
Position Strand Gene LocusTag Fraction Methanol (C) remove 231,198 - GFF994 0.57 +0.3 231,210 + GFF994 0.58 -1.2 231,210 + GFF994 0.58 -1.0 231,210 + GFF994 0.58 -1.3 231,210 + GFF994 0.58 +0.7 231,211 - GFF994 0.58 -0.2 231,211 - GFF994 0.58 +0.1 231,211 - GFF994 0.58 +0.4 231,211 - GFF994 0.58 -0.0 231,211 - GFF994 0.58 +0.3 231,211 - GFF994 0.58 -0.3 231,211 - GFF994 0.58 -0.0 231,217 + GFF994 0.58 -0.0 231,217 + GFF994 0.58 -0.9 231,217 + GFF994 0.58 -0.8 231,218 - GFF994 0.58 +0.3 231,251 + GFF994 0.60 -1.3 231,251 + GFF994 0.60 -0.3 231,252 - GFF994 0.60 -0.8 231,281 - GFF994 0.61 -0.1 231,342 + GFF994 0.64 +0.8 231,342 + GFF994 0.64 +0.4 231,342 + GFF994 0.64 +0.4 231,342 + GFF994 0.64 -1.3 231,342 + GFF994 0.64 -0.0 231,343 - GFF994 0.64 +0.8 231,483 + GFF994 0.71 +0.1 231,483 + GFF994 0.71 -0.6 231,484 - GFF994 0.71 -0.7 231,565 - GFF994 0.76 +0.1 231,623 + GFF994 0.79 -1.6 231,623 + GFF994 0.79 -0.4 231,623 + GFF994 0.79 -1.0 231,623 + GFF994 0.79 -1.3 231,623 + GFF994 0.79 -0.3 231,623 + GFF994 0.79 -1.3 231,624 - GFF994 0.79 +0.2 231,624 - GFF994 0.79 -0.3 231,624 - GFF994 0.79 -0.3 231,624 - GFF994 0.79 +1.0 231,624 - GFF994 0.79 -1.1 231,624 - GFF994 0.79 +0.6 231,624 - GFF994 0.79 -1.4 231,627 + GFF994 0.79 -1.8 231,627 + GFF994 0.79 -0.0 231,627 + GFF994 0.79 -0.6 231,628 - GFF994 0.79 -1.0 231,628 - GFF994 0.79 +0.6 231,628 - GFF994 0.79 -0.5 231,628 - GFF994 0.79 +0.7 231,628 - GFF994 0.79 -0.6 231,628 - GFF994 0.79 -1.0 231,628 - GFF994 0.79 +0.7 231,696 - GFF994 0.82 -1.0 231,707 + GFF994 0.83 -1.0 231,708 - GFF994 0.83 +0.6 231,708 - GFF994 0.83 -1.0 231,747 + GFF994 0.85 -0.9 231,748 - GFF994 0.85 -1.3 231,808 + GFF994 0.88 +0.3 231,809 - GFF994 0.88 +0.1 231,809 - GFF994 0.88 -0.1 231,809 - GFF994 0.88 -0.6 231,809 - GFF994 0.88 -0.4 231,833 + GFF994 0.89 +1.0 231,833 + GFF994 0.89 +0.1 231,833 + GFF994 0.89 -1.1 231,834 - GFF994 0.89 -1.0 231,834 - GFF994 0.89 +1.0 231,834 - GFF994 0.89 -1.0 231,868 - -1.9 231,868 - -0.8 231,886 + -1.5 231,887 - +0.2 231,889 + -0.8 231,890 - -2.2 231,890 - -1.0 231,923 + -0.6 231,923 + -1.3 231,924 - -1.6 231,956 + -1.0 231,956 + +0.5 231,956 + -1.2 231,957 - -0.6 231,957 - -0.0 232,013 - -0.3 232,030 + -1.3 232,030 + -0.5 232,030 + -0.3 232,041 + -1.5 232,047 + -0.1 232,047 + +0.6 232,047 + -0.8 232,047 + -1.2 232,047 + -0.3 232,048 - +0.2 232,048 - -0.2 232,048 - -0.4 232,048 - -1.4 232,104 - +0.1 232,106 + -1.1 232,106 + +0.4 232,106 + -0.3 232,106 + +0.6 232,106 + -0.8 232,106 + -0.0 232,106 + -1.6 232,107 - -3.1 232,107 - -0.4 232,107 - +0.5 232,107 - -0.0 232,150 - -0.9 232,152 + +0.2 232,152 + -0.0 232,153 - -0.6 232,153 - +0.2 232,312 - -0.6 232,313 + -1.3 232,324 + -1.0 232,353 + +0.2 232,527 - GFF995 0.13 +0.4 232,527 - GFF995 0.13 -0.8 232,547 - GFF995 0.14 +0.2 232,547 - GFF995 0.14 +0.6 232,547 - GFF995 0.14 +1.3 232,550 + GFF995 0.14 -1.3 232,550 + GFF995 0.14 -0.3 232,551 - GFF995 0.14 -0.6 232,551 - GFF995 0.14 -1.3 232,645 + GFF995 0.17 -0.4 232,647 + GFF995 0.18 -0.2 232,647 + GFF995 0.18 -1.6 232,647 + GFF995 0.18 -1.3 232,647 + GFF995 0.18 +0.2 232,647 + GFF995 0.18 -1.0 232,647 + GFF995 0.18 -0.3 232,648 - GFF995 0.18 -2.0 232,860 - GFF995 0.25 -1.6 232,868 + GFF995 0.26 +0.7 232,868 + GFF995 0.26 -0.6 232,900 + GFF995 0.27 -1.8 232,970 + GFF995 0.29 +0.5 232,970 + GFF995 0.29 -1.3 232,988 + GFF995 0.30 -0.5 232,988 + GFF995 0.30 -0.4 232,989 - GFF995 0.30 -0.2 232,989 - GFF995 0.30 -1.3 232,989 - GFF995 0.30 -1.0 232,989 - GFF995 0.30 -0.0 233,056 + GFF995 0.32 -0.3 233,056 + GFF995 0.32 -0.0 233,057 - GFF995 0.33 -0.8 233,231 + GFF995 0.39 -0.0 233,237 + GFF995 0.39 -1.6 233,258 - GFF995 0.40 -0.0 233,267 + GFF995 0.40 -0.4 233,312 + GFF995 0.42 -1.0 233,312 + GFF995 0.42 -1.0 233,363 - GFF995 0.44 -1.6 233,383 + GFF995 0.44 -0.6 233,384 - GFF995 0.44 -0.2 233,394 + GFF995 0.45 +0.0 233,394 + GFF995 0.45 -2.0 233,394 + GFF995 0.45 -1.0 233,442 + GFF995 0.47 -1.8 233,442 + GFF995 0.47 -1.4 233,516 + GFF995 0.49 -1.0 233,626 - GFF995 0.53 -0.0 233,723 + GFF995 0.57 -1.8 233,804 + GFF995 0.60 -1.0 233,898 + GFF995 0.63 -0.3 233,898 + GFF995 0.63 -1.3 234,167 + GFF995 0.73 -0.8 234,187 + GFF995 0.74 -2.3 234,256 + GFF995 0.76 -1.3 234,346 + GFF995 0.80 +0.2 234,368 + GFF995 0.80 -0.0 234,379 + GFF995 0.81 -1.6 234,616 + GFF995 0.89 +0.2 234,649 + -1.0 234,873 + -1.6 234,873 + -0.0 234,874 - -0.0 234,874 - +0.2 234,874 - +0.2 234,919 - +1.0 234,975 + -0.2 234,975 + -0.2 234,975 + -1.7 235,014 + -0.8 235,036 + +0.2 235,036 + +0.1 235,036 + -0.9 235,036 + +0.4 235,036 + -0.8 235,036 + +0.9 235,036 + -1.0 235,036 + +0.2 235,036 + -1.1 235,036 + -0.0 235,036 + +0.1 235,036 + -0.6 235,036 + -1.7 235,036 + +0.3 235,036 + +0.4 235,036 + -1.3 235,037 - -0.6 235,037 - -0.9 235,037 - +0.6 235,037 - -0.3 235,037 - -0.9 235,037 - -0.4 235,037 - -0.6 235,037 - -0.1 235,037 - -1.1 235,037 - -2.6 235,038 + -0.8 235,039 - -1.3 235,050 + -1.3 235,051 - -1.0 235,056 + +0.3 235,057 - -0.9 235,057 - -1.0 235,057 - -0.3 235,057 - -0.7 235,057 - +0.6 235,081 + -1.6 235,081 + +0.4 235,082 - +0.4 235,082 - -1.0 235,082 - -0.0 235,082 - -1.2 235,082 - +0.0 235,082 - -1.0 235,082 - +0.1 235,082 - -0.2 235,082 - +0.5 235,085 + -2.0 235,085 + -0.9 235,086 - +0.1 235,086 - +1.0 235,086 - -0.2 235,087 + -1.0 235,087 + +1.3 235,087 + +0.2 235,088 - -0.3 235,136 + GFF996 0.11 +0.4 235,136 + GFF996 0.11 -0.8 235,136 + GFF996 0.11 -0.2 235,137 - GFF996 0.11 -1.8 235,154 - GFF996 0.12 -0.3 235,235 - GFF996 0.16 -0.0 235,401 - GFF996 0.25 -1.6 235,420 - GFF996 0.26 +0.1 235,420 - GFF996 0.26 -0.9 235,457 + GFF996 0.27 -0.6 235,458 - GFF996 0.27 -0.6 235,458 - GFF996 0.27 -0.3 235,458 - GFF996 0.27 -1.4 235,517 + GFF996 0.30 +1.0 235,630 + GFF996 0.36 +1.2 235,643 + GFF996 0.37 -0.0 235,669 - GFF996 0.38 -2.2 235,702 + GFF996 0.40 -0.0 235,703 - GFF996 0.40 -1.8 235,703 - GFF996 0.40 -1.3 235,703 - GFF996 0.40 -1.3 235,703 - GFF996 0.40 -1.6 235,746 + GFF996 0.42 -1.6 235,746 + GFF996 0.42 -0.0 235,747 - GFF996 0.42 -2.5 235,763 + GFF996 0.43 +0.3 235,764 - GFF996 0.43 -0.8 235,764 - GFF996 0.43 +0.2 235,764 - GFF996 0.43 -0.1 235,764 - GFF996 0.43 -0.0 235,764 - GFF996 0.43 -0.5 235,779 - GFF996 0.44 -0.9 235,779 - GFF996 0.44 -0.5 235,779 - GFF996 0.44 -0.0 235,842 + GFF996 0.47 +0.2 235,874 + GFF996 0.49 -0.2 235,874 + GFF996 0.49 -1.2 235,875 - GFF996 0.49 -0.3 235,875 - GFF996 0.49 +0.2 235,875 - GFF996 0.49 +1.0 235,875 - GFF996 0.49 -0.4 235,875 - GFF996 0.49 -1.0 235,875 - GFF996 0.49 -1.0 235,875 - GFF996 0.49 -1.0
Or see this region's nucleotide sequence