Strain Fitness in Methylophilus sp. DMC18 around GFF2327

Experiment: Methanol (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2325 and GFF2326 overlap by 4 nucleotidesGFF2326 and GFF2327 are separated by 129 nucleotidesGFF2327 and GFF2328 are separated by 1 nucleotidesGFF2328 and GFF2329 overlap by 4 nucleotides GFF2325 - Crossover junction endodeoxyribonuclease RuvC, at 40,859 to 41,419 GFF2325 GFF2326 - Holliday junction ATP-dependent DNA helicase RuvA, at 41,416 to 41,991 GFF2326 GFF2327 - Holliday junction ATP-dependent DNA helicase RuvB, at 42,121 to 43,167 GFF2327 GFF2328 - Acyl-CoA thioesterase YbgC, at 43,169 to 43,564 GFF2328 GFF2329 - Tol-Pal system protein TolQ, at 43,561 to 44,235 GFF2329 Position (kb) 42 43 44Strain fitness (log2 ratio) -2 -1 0 1at 41.202 kb on - strand, within GFF2325at 41.287 kb on + strand, within GFF2325at 41.287 kb on + strand, within GFF2325at 41.367 kb on + strandat 41.367 kb on + strandat 41.398 kb on + strandat 41.398 kb on + strandat 41.398 kb on + strandat 41.399 kb on - strandat 41.415 kb on + strandat 41.415 kb on + strandat 41.416 kb on - strandat 41.492 kb on - strand, within GFF2326at 41.492 kb on - strand, within GFF2326at 41.498 kb on + strand, within GFF2326at 41.565 kb on + strand, within GFF2326at 41.565 kb on + strand, within GFF2326at 41.566 kb on - strand, within GFF2326at 41.566 kb on - strand, within GFF2326at 41.596 kb on + strand, within GFF2326at 41.596 kb on + strand, within GFF2326at 41.596 kb on + strand, within GFF2326at 41.752 kb on - strand, within GFF2326at 41.866 kb on - strand, within GFF2326at 41.977 kb on + strandat 42.017 kb on + strandat 42.017 kb on + strandat 42.018 kb on - strandat 42.018 kb on - strandat 42.020 kb on + strandat 42.020 kb on + strandat 42.021 kb on - strandat 42.021 kb on - strandat 42.026 kb on + strandat 42.026 kb on + strandat 42.026 kb on + strandat 42.027 kb on - strandat 42.027 kb on - strandat 42.027 kb on - strandat 42.027 kb on - strandat 42.027 kb on - strandat 42.027 kb on - strandat 42.027 kb on - strandat 42.038 kb on + strandat 42.038 kb on + strandat 42.038 kb on + strandat 42.038 kb on + strandat 42.039 kb on - strandat 42.052 kb on + strandat 42.091 kb on + strandat 42.091 kb on + strandat 42.214 kb on + strandat 42.214 kb on + strandat 42.215 kb on - strandat 42.215 kb on - strandat 42.456 kb on + strand, within GFF2327at 42.926 kb on + strand, within GFF2327at 42.926 kb on + strand, within GFF2327at 43.013 kb on + strand, within GFF2327at 43.096 kb on + strandat 43.096 kb on + strandat 43.165 kb on + strandat 43.193 kb on + strandat 43.194 kb on - strandat 43.196 kb on + strandat 43.196 kb on + strandat 43.226 kb on + strand, within GFF2328at 43.239 kb on - strand, within GFF2328at 43.350 kb on + strand, within GFF2328at 43.350 kb on + strand, within GFF2328at 43.350 kb on + strand, within GFF2328at 43.351 kb on - strand, within GFF2328at 43.357 kb on + strand, within GFF2328at 43.467 kb on + strand, within GFF2328at 43.467 kb on + strand, within GFF2328at 43.513 kb on + strand, within GFF2328

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Per-strain Table

Position Strand Gene LocusTag Fraction Methanol (C)
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41,202 - GFF2325 0.61 -2.3
41,287 + GFF2325 0.76 -1.3
41,287 + GFF2325 0.76 -1.8
41,367 + +0.1
41,367 + -0.2
41,398 + -1.3
41,398 + -0.5
41,398 + -1.8
41,399 - -0.3
41,415 + -0.3
41,415 + -1.6
41,416 - -1.0
41,492 - GFF2326 0.13 -2.3
41,492 - GFF2326 0.13 -1.0
41,498 + GFF2326 0.14 -1.1
41,565 + GFF2326 0.26 -1.0
41,565 + GFF2326 0.26 -0.6
41,566 - GFF2326 0.26 -1.3
41,566 - GFF2326 0.26 -1.0
41,596 + GFF2326 0.31 -0.6
41,596 + GFF2326 0.31 -0.6
41,596 + GFF2326 0.31 +0.4
41,752 - GFF2326 0.58 -0.9
41,866 - GFF2326 0.78 -1.3
41,977 + +0.6
42,017 + -1.8
42,017 + -1.0
42,018 - +0.6
42,018 - -0.3
42,020 + -0.9
42,020 + -1.0
42,021 - -0.0
42,021 - -0.5
42,026 + +0.2
42,026 + -0.4
42,026 + +1.0
42,027 - -1.0
42,027 - -0.3
42,027 - +1.2
42,027 - -0.1
42,027 - -0.7
42,027 - +0.2
42,027 - +1.0
42,038 + -1.0
42,038 + +0.3
42,038 + -0.6
42,038 + +0.4
42,039 - -1.8
42,052 + -0.6
42,091 + -1.2
42,091 + -0.3
42,214 + -0.8
42,214 + -0.6
42,215 - -1.3
42,215 - -1.0
42,456 + GFF2327 0.32 -1.5
42,926 + GFF2327 0.77 -1.3
42,926 + GFF2327 0.77 -1.5
43,013 + GFF2327 0.85 +0.7
43,096 + +1.0
43,096 + -1.3
43,165 + -0.3
43,193 + -0.2
43,194 - -0.3
43,196 + -0.6
43,196 + -0.3
43,226 + GFF2328 0.14 +0.3
43,239 - GFF2328 0.18 -1.5
43,350 + GFF2328 0.46 -1.8
43,350 + GFF2328 0.46 -1.0
43,350 + GFF2328 0.46 +0.3
43,351 - GFF2328 0.46 +0.2
43,357 + GFF2328 0.47 +0.1
43,467 + GFF2328 0.75 -0.2
43,467 + GFF2328 0.75 -0.5
43,513 + GFF2328 0.87 -0.5

Or see this region's nucleotide sequence