Strain Fitness in Methylophilus sp. DMC18 around GFF203

Experiment: Methanol (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF201 and GFF202 are separated by 27 nucleotidesGFF202 and GFF203 are separated by 38 nucleotidesGFF203 and GFF204 are separated by 54 nucleotides GFF201 - Periplasmic nitrate reductase, at 202,014 to 204,782 GFF201 GFF202 - Assimilatory nitrite reductase [NAD(P)H] small subunit, at 204,810 to 205,121 GFF202 GFF203 - Serine/threonine-protein kinase PknD, at 205,160 to 206,848 GFF203 GFF204 - Nitrate transporter, at 206,903 to 208,114 GFF204 Position (kb) 205 206 207Strain fitness (log2 ratio) -3 -2 -1 0 1at 204.224 kb on + strand, within GFF201at 204.242 kb on - strand, within GFF201at 204.242 kb on - strand, within GFF201at 204.242 kb on - strand, within GFF201at 204.242 kb on - strand, within GFF201at 204.362 kb on + strand, within GFF201at 204.362 kb on + strand, within GFF201at 204.366 kb on + strand, within GFF201at 204.366 kb on + strand, within GFF201at 204.366 kb on + strand, within GFF201at 204.366 kb on + strand, within GFF201at 204.366 kb on + strand, within GFF201at 204.367 kb on - strand, within GFF201at 204.367 kb on - strand, within GFF201at 204.367 kb on - strand, within GFF201at 204.367 kb on - strand, within GFF201at 204.370 kb on + strand, within GFF201at 204.370 kb on + strand, within GFF201at 204.370 kb on + strand, within GFF201at 204.370 kb on + strand, within GFF201at 204.370 kb on + strand, within GFF201at 204.370 kb on + strand, within GFF201at 204.370 kb on + strand, within GFF201at 204.370 kb on + strand, within GFF201at 204.371 kb on - strand, within GFF201at 204.371 kb on - strand, within GFF201at 204.371 kb on - strand, within GFF201at 204.371 kb on - strand, within GFF201at 204.371 kb on - strand, within GFF201at 204.373 kb on + strand, within GFF201at 204.374 kb on - strand, within GFF201at 204.374 kb on - strand, within GFF201at 204.374 kb on - strand, within GFF201at 204.374 kb on - strand, within GFF201at 204.401 kb on + strand, within GFF201at 204.403 kb on + strand, within GFF201at 204.404 kb on - strand, within GFF201at 204.404 kb on - strand, within GFF201at 204.437 kb on + strand, within GFF201at 204.476 kb on + strand, within GFF201at 204.653 kb on + strandat 204.682 kb on + strandat 204.683 kb on - strandat 204.782 kb on + strandat 204.782 kb on + strandat 204.811 kb on + strandat 204.819 kb on + strandat 204.819 kb on + strandat 204.820 kb on - strandat 204.829 kb on + strandat 204.829 kb on + strandat 204.830 kb on - strandat 204.830 kb on - strandat 204.830 kb on - strandat 204.859 kb on + strand, within GFF202at 204.923 kb on + strand, within GFF202at 204.923 kb on + strand, within GFF202at 204.923 kb on + strand, within GFF202at 204.923 kb on + strand, within GFF202at 204.924 kb on - strand, within GFF202at 204.924 kb on - strand, within GFF202at 204.924 kb on - strand, within GFF202at 204.924 kb on - strand, within GFF202at 204.924 kb on - strand, within GFF202at 204.924 kb on - strand, within GFF202at 204.952 kb on + strand, within GFF202at 205.162 kb on - strandat 205.179 kb on + strandat 205.211 kb on - strandat 205.235 kb on + strandat 205.261 kb on + strandat 205.366 kb on + strand, within GFF203at 205.366 kb on + strand, within GFF203at 205.467 kb on + strand, within GFF203at 205.467 kb on + strand, within GFF203at 205.468 kb on - strand, within GFF203at 205.468 kb on - strand, within GFF203at 205.468 kb on - strand, within GFF203at 205.471 kb on + strand, within GFF203at 205.488 kb on + strand, within GFF203at 205.488 kb on + strand, within GFF203at 205.488 kb on + strand, within GFF203at 205.488 kb on + strand, within GFF203at 205.489 kb on - strand, within GFF203at 205.509 kb on + strand, within GFF203at 205.514 kb on - strand, within GFF203at 205.651 kb on + strand, within GFF203at 205.669 kb on + strand, within GFF203at 205.700 kb on - strand, within GFF203at 205.830 kb on + strand, within GFF203at 205.830 kb on + strand, within GFF203at 205.833 kb on + strand, within GFF203at 205.833 kb on + strand, within GFF203at 205.942 kb on + strand, within GFF203at 205.983 kb on - strand, within GFF203at 205.983 kb on - strand, within GFF203at 206.010 kb on + strand, within GFF203at 206.010 kb on + strand, within GFF203at 206.011 kb on - strand, within GFF203at 206.011 kb on - strand, within GFF203at 206.012 kb on + strand, within GFF203at 206.012 kb on + strand, within GFF203at 206.013 kb on + strand, within GFF203at 206.013 kb on - strand, within GFF203at 206.013 kb on - strand, within GFF203at 206.036 kb on + strand, within GFF203at 206.036 kb on + strand, within GFF203at 206.036 kb on + strand, within GFF203at 206.037 kb on - strand, within GFF203at 206.037 kb on - strand, within GFF203at 206.239 kb on + strand, within GFF203at 206.240 kb on - strand, within GFF203at 206.240 kb on - strand, within GFF203at 206.240 kb on - strand, within GFF203at 206.244 kb on + strand, within GFF203at 206.245 kb on - strand, within GFF203at 206.256 kb on - strand, within GFF203at 206.256 kb on - strand, within GFF203at 206.313 kb on + strand, within GFF203at 206.313 kb on + strand, within GFF203at 206.313 kb on + strand, within GFF203at 206.314 kb on - strand, within GFF203at 206.314 kb on - strand, within GFF203at 206.347 kb on - strand, within GFF203at 206.390 kb on + strand, within GFF203at 206.390 kb on + strand, within GFF203at 206.391 kb on - strand, within GFF203at 206.391 kb on - strand, within GFF203at 206.391 kb on - strand, within GFF203at 206.391 kb on - strand, within GFF203at 206.463 kb on + strand, within GFF203at 206.497 kb on - strand, within GFF203at 206.646 kb on + strand, within GFF203at 206.649 kb on + strand, within GFF203at 206.650 kb on - strand, within GFF203at 206.650 kb on - strand, within GFF203at 206.784 kb on + strandat 206.841 kb on - strandat 207.024 kb on + strandat 207.049 kb on - strand, within GFF204at 207.070 kb on - strand, within GFF204at 207.198 kb on - strand, within GFF204at 207.200 kb on + strand, within GFF204at 207.201 kb on - strand, within GFF204at 207.212 kb on + strand, within GFF204at 207.212 kb on + strand, within GFF204at 207.270 kb on + strand, within GFF204at 207.270 kb on + strand, within GFF204at 207.270 kb on + strand, within GFF204at 207.270 kb on + strand, within GFF204at 207.270 kb on + strand, within GFF204at 207.270 kb on + strand, within GFF204at 207.270 kb on + strand, within GFF204at 207.270 kb on + strand, within GFF204at 207.270 kb on + strand, within GFF204at 207.270 kb on + strand, within GFF204at 207.270 kb on + strand, within GFF204at 207.270 kb on + strand, within GFF204at 207.270 kb on + strand, within GFF204at 207.271 kb on - strand, within GFF204at 207.271 kb on - strand, within GFF204at 207.271 kb on - strand, within GFF204at 207.271 kb on - strand, within GFF204at 207.271 kb on - strand, within GFF204at 207.271 kb on - strand, within GFF204at 207.271 kb on - strand, within GFF204at 207.271 kb on - strand, within GFF204at 207.271 kb on - strand, within GFF204at 207.271 kb on - strand, within GFF204at 207.271 kb on - strand, within GFF204at 207.271 kb on - strand, within GFF204at 207.271 kb on - strand, within GFF204at 207.271 kb on - strand, within GFF204at 207.323 kb on + strand, within GFF204at 207.370 kb on - strand, within GFF204at 207.396 kb on + strand, within GFF204at 207.397 kb on - strand, within GFF204at 207.411 kb on + strand, within GFF204at 207.412 kb on - strand, within GFF204at 207.412 kb on - strand, within GFF204at 207.473 kb on - strand, within GFF204at 207.490 kb on + strand, within GFF204at 207.511 kb on - strand, within GFF204at 207.567 kb on + strand, within GFF204at 207.567 kb on + strand, within GFF204at 207.568 kb on - strand, within GFF204at 207.611 kb on + strand, within GFF204at 207.611 kb on + strand, within GFF204at 207.616 kb on + strand, within GFF204at 207.617 kb on - strand, within GFF204at 207.617 kb on - strand, within GFF204at 207.679 kb on + strand, within GFF204at 207.791 kb on + strand, within GFF204at 207.792 kb on - strand, within GFF204at 207.792 kb on - strand, within GFF204at 207.810 kb on + strand, within GFF204at 207.814 kb on + strand, within GFF204at 207.814 kb on + strand, within GFF204at 207.814 kb on + strand, within GFF204at 207.814 kb on + strand, within GFF204at 207.814 kb on + strand, within GFF204at 207.814 kb on + strand, within GFF204at 207.814 kb on + strand, within GFF204at 207.814 kb on + strand, within GFF204at 207.815 kb on - strand, within GFF204at 207.815 kb on - strand, within GFF204at 207.815 kb on - strand, within GFF204at 207.815 kb on - strand, within GFF204at 207.815 kb on - strand, within GFF204at 207.815 kb on - strand, within GFF204at 207.815 kb on - strand, within GFF204at 207.815 kb on - strand, within GFF204

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Per-strain Table

Position Strand Gene LocusTag Fraction Methanol (C)
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204,224 + GFF201 0.80 +0.3
204,242 - GFF201 0.80 -1.6
204,242 - GFF201 0.80 +0.5
204,242 - GFF201 0.80 -0.3
204,242 - GFF201 0.80 -2.5
204,362 + GFF201 0.85 -0.2
204,362 + GFF201 0.85 -0.4
204,366 + GFF201 0.85 -0.3
204,366 + GFF201 0.85 -0.7
204,366 + GFF201 0.85 -1.0
204,366 + GFF201 0.85 -1.0
204,366 + GFF201 0.85 -0.8
204,367 - GFF201 0.85 -0.1
204,367 - GFF201 0.85 +0.0
204,367 - GFF201 0.85 -2.0
204,367 - GFF201 0.85 -1.0
204,370 + GFF201 0.85 -2.5
204,370 + GFF201 0.85 -0.8
204,370 + GFF201 0.85 -1.5
204,370 + GFF201 0.85 -1.0
204,370 + GFF201 0.85 -1.0
204,370 + GFF201 0.85 -1.1
204,370 + GFF201 0.85 -0.9
204,370 + GFF201 0.85 -0.8
204,371 - GFF201 0.85 +0.7
204,371 - GFF201 0.85 -1.9
204,371 - GFF201 0.85 +0.0
204,371 - GFF201 0.85 -0.8
204,371 - GFF201 0.85 -1.0
204,373 + GFF201 0.85 -0.3
204,374 - GFF201 0.85 -1.1
204,374 - GFF201 0.85 -1.2
204,374 - GFF201 0.85 -0.5
204,374 - GFF201 0.85 -0.5
204,401 + GFF201 0.86 -1.6
204,403 + GFF201 0.86 -1.3
204,404 - GFF201 0.86 -0.9
204,404 - GFF201 0.86 -1.0
204,437 + GFF201 0.88 -1.5
204,476 + GFF201 0.89 -2.6
204,653 + -1.2
204,682 + -2.6
204,683 - -1.6
204,782 + -0.3
204,782 + -0.7
204,811 + -1.3
204,819 + -1.1
204,819 + -0.2
204,820 - -1.3
204,829 + -0.3
204,829 + -1.0
204,830 - +0.4
204,830 - -0.6
204,830 - -0.5
204,859 + GFF202 0.16 -0.8
204,923 + GFF202 0.36 -1.8
204,923 + GFF202 0.36 -1.2
204,923 + GFF202 0.36 -1.5
204,923 + GFF202 0.36 -1.3
204,924 - GFF202 0.37 -1.0
204,924 - GFF202 0.37 -0.8
204,924 - GFF202 0.37 -0.7
204,924 - GFF202 0.37 -1.6
204,924 - GFF202 0.37 -0.2
204,924 - GFF202 0.37 -1.1
204,952 + GFF202 0.46 -0.3
205,162 - -0.2
205,179 + -1.1
205,211 - -0.2
205,235 + +0.0
205,261 + -0.6
205,366 + GFF203 0.12 +1.0
205,366 + GFF203 0.12 +0.0
205,467 + GFF203 0.18 -0.6
205,467 + GFF203 0.18 -0.7
205,468 - GFF203 0.18 -0.3
205,468 - GFF203 0.18 -1.1
205,468 - GFF203 0.18 -1.0
205,471 + GFF203 0.18 -0.4
205,488 + GFF203 0.19 +0.3
205,488 + GFF203 0.19 -0.4
205,488 + GFF203 0.19 -1.3
205,488 + GFF203 0.19 +0.0
205,489 - GFF203 0.19 -0.8
205,509 + GFF203 0.21 -2.3
205,514 - GFF203 0.21 +0.0
205,651 + GFF203 0.29 -2.3
205,669 + GFF203 0.30 -1.0
205,700 - GFF203 0.32 +0.3
205,830 + GFF203 0.40 +0.7
205,830 + GFF203 0.40 -1.6
205,833 + GFF203 0.40 -1.8
205,833 + GFF203 0.40 +0.4
205,942 + GFF203 0.46 -1.3
205,983 - GFF203 0.49 -0.4
205,983 - GFF203 0.49 +0.2
206,010 + GFF203 0.50 +0.0
206,010 + GFF203 0.50 -0.6
206,011 - GFF203 0.50 -1.6
206,011 - GFF203 0.50 +0.0
206,012 + GFF203 0.50 +0.0
206,012 + GFF203 0.50 -2.9
206,013 + GFF203 0.51 -1.0
206,013 - GFF203 0.51 +0.7
206,013 - GFF203 0.51 -0.1
206,036 + GFF203 0.52 +0.3
206,036 + GFF203 0.52 -1.8
206,036 + GFF203 0.52 +0.0
206,037 - GFF203 0.52 -0.5
206,037 - GFF203 0.52 -1.8
206,239 + GFF203 0.64 -2.5
206,240 - GFF203 0.64 -0.3
206,240 - GFF203 0.64 +0.0
206,240 - GFF203 0.64 -1.3
206,244 + GFF203 0.64 -0.3
206,245 - GFF203 0.64 -2.7
206,256 - GFF203 0.65 -0.3
206,256 - GFF203 0.65 -1.4
206,313 + GFF203 0.68 -1.0
206,313 + GFF203 0.68 -0.6
206,313 + GFF203 0.68 -0.7
206,314 - GFF203 0.68 -1.2
206,314 - GFF203 0.68 -1.0
206,347 - GFF203 0.70 -0.2
206,390 + GFF203 0.73 -0.2
206,390 + GFF203 0.73 -0.7
206,391 - GFF203 0.73 -0.6
206,391 - GFF203 0.73 +0.0
206,391 - GFF203 0.73 -1.0
206,391 - GFF203 0.73 -0.3
206,463 + GFF203 0.77 -1.3
206,497 - GFF203 0.79 -1.0
206,646 + GFF203 0.88 -1.2
206,649 + GFF203 0.88 -2.0
206,650 - GFF203 0.88 -0.6
206,650 - GFF203 0.88 -0.2
206,784 + -2.3
206,841 - -0.9
207,024 + -0.6
207,049 - GFF204 0.12 +0.0
207,070 - GFF204 0.14 -0.7
207,198 - GFF204 0.24 -0.4
207,200 + GFF204 0.25 -0.1
207,201 - GFF204 0.25 -0.2
207,212 + GFF204 0.25 -2.2
207,212 + GFF204 0.25 -1.1
207,270 + GFF204 0.30 -0.5
207,270 + GFF204 0.30 -1.6
207,270 + GFF204 0.30 -1.0
207,270 + GFF204 0.30 -1.2
207,270 + GFF204 0.30 -0.5
207,270 + GFF204 0.30 -1.0
207,270 + GFF204 0.30 -0.3
207,270 + GFF204 0.30 +0.0
207,270 + GFF204 0.30 -0.4
207,270 + GFF204 0.30 -0.3
207,270 + GFF204 0.30 -0.1
207,270 + GFF204 0.30 -1.8
207,270 + GFF204 0.30 -0.6
207,271 - GFF204 0.30 -1.4
207,271 - GFF204 0.30 -2.5
207,271 - GFF204 0.30 -0.4
207,271 - GFF204 0.30 +0.7
207,271 - GFF204 0.30 -0.8
207,271 - GFF204 0.30 -1.0
207,271 - GFF204 0.30 +1.0
207,271 - GFF204 0.30 -1.3
207,271 - GFF204 0.30 -0.7
207,271 - GFF204 0.30 -1.6
207,271 - GFF204 0.30 -0.1
207,271 - GFF204 0.30 -0.3
207,271 - GFF204 0.30 +0.0
207,271 - GFF204 0.30 -0.2
207,323 + GFF204 0.35 +0.0
207,370 - GFF204 0.39 -1.6
207,396 + GFF204 0.41 +1.3
207,397 - GFF204 0.41 +0.0
207,411 + GFF204 0.42 +1.3
207,412 - GFF204 0.42 +0.0
207,412 - GFF204 0.42 +0.4
207,473 - GFF204 0.47 -0.7
207,490 + GFF204 0.48 -0.6
207,511 - GFF204 0.50 +0.8
207,567 + GFF204 0.55 -0.3
207,567 + GFF204 0.55 -1.3
207,568 - GFF204 0.55 -1.3
207,611 + GFF204 0.58 +0.3
207,611 + GFF204 0.58 -1.0
207,616 + GFF204 0.59 +0.7
207,617 - GFF204 0.59 -1.6
207,617 - GFF204 0.59 -1.6
207,679 + GFF204 0.64 +1.0
207,791 + GFF204 0.73 -0.8
207,792 - GFF204 0.73 -1.6
207,792 - GFF204 0.73 -1.5
207,810 + GFF204 0.75 -2.0
207,814 + GFF204 0.75 -2.0
207,814 + GFF204 0.75 +0.7
207,814 + GFF204 0.75 -2.0
207,814 + GFF204 0.75 -0.5
207,814 + GFF204 0.75 -1.9
207,814 + GFF204 0.75 -0.8
207,814 + GFF204 0.75 +1.0
207,814 + GFF204 0.75 -0.6
207,815 - GFF204 0.75 -1.0
207,815 - GFF204 0.75 -1.8
207,815 - GFF204 0.75 -0.2
207,815 - GFF204 0.75 -1.6
207,815 - GFF204 0.75 +0.1
207,815 - GFF204 0.75 -1.2
207,815 - GFF204 0.75 -1.2
207,815 - GFF204 0.75 -2.5

Or see this region's nucleotide sequence