Experiment: MGL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX19_06218 and MPMX19_06219 are separated by 457 nucleotides MPMX19_06219 and MPMX19_06220 are separated by 196 nucleotides
MPMX19_06218: MPMX19_06218 - ABC transporter periplasmic-binding protein YtfQ, at 401,475 to 402,443
_06218
MPMX19_06219: MPMX19_06219 - hypothetical protein, at 402,901 to 404,058
_06219
MPMX19_06220: MPMX19_06220 - Alpha-ketoglutaric semialdehyde dehydrogenase 2, at 404,255 to 405,835
_06220
Position (kb)
402
403
404
405 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 402.061 kb on + strand, within MPMX19_06218 at 402.061 kb on + strand, within MPMX19_06218 at 402.061 kb on + strand, within MPMX19_06218 at 402.061 kb on + strand, within MPMX19_06218 at 402.061 kb on + strand, within MPMX19_06218 at 402.061 kb on + strand, within MPMX19_06218 at 402.061 kb on + strand, within MPMX19_06218 at 402.061 kb on + strand, within MPMX19_06218 at 402.061 kb on + strand, within MPMX19_06218 at 402.061 kb on + strand, within MPMX19_06218 at 402.061 kb on + strand, within MPMX19_06218 at 402.061 kb on + strand, within MPMX19_06218 at 402.061 kb on + strand, within MPMX19_06218 at 402.061 kb on + strand, within MPMX19_06218 at 402.061 kb on + strand, within MPMX19_06218 at 402.061 kb on + strand, within MPMX19_06218 at 402.061 kb on + strand, within MPMX19_06218 at 402.061 kb on + strand, within MPMX19_06218 at 402.062 kb on - strand, within MPMX19_06218 at 402.062 kb on - strand, within MPMX19_06218 at 402.062 kb on - strand, within MPMX19_06218 at 402.062 kb on - strand, within MPMX19_06218 at 402.062 kb on - strand, within MPMX19_06218 at 402.062 kb on - strand, within MPMX19_06218 at 402.062 kb on - strand, within MPMX19_06218 at 402.062 kb on - strand, within MPMX19_06218 at 402.062 kb on - strand, within MPMX19_06218 at 402.062 kb on - strand, within MPMX19_06218 at 402.062 kb on - strand, within MPMX19_06218 at 402.062 kb on - strand, within MPMX19_06218 at 402.062 kb on - strand, within MPMX19_06218 at 402.404 kb on + strand at 402.464 kb on + strand at 402.464 kb on + strand at 402.464 kb on + strand at 402.476 kb on + strand at 402.476 kb on + strand at 402.476 kb on + strand at 402.476 kb on + strand at 402.476 kb on + strand at 402.476 kb on + strand at 402.476 kb on + strand at 402.477 kb on - strand at 402.477 kb on - strand at 402.477 kb on - strand at 402.477 kb on - strand at 402.496 kb on - strand at 402.509 kb on + strand at 402.510 kb on - strand at 402.523 kb on + strand at 402.523 kb on + strand at 402.523 kb on + strand at 402.608 kb on - strand at 402.667 kb on + strand at 402.668 kb on - strand at 402.669 kb on + strand at 402.669 kb on + strand at 402.669 kb on + strand at 402.669 kb on + strand at 402.669 kb on + strand at 402.669 kb on + strand at 402.670 kb on - strand at 402.670 kb on - strand at 402.670 kb on - strand at 402.670 kb on - strand at 402.670 kb on - strand at 402.670 kb on - strand at 402.670 kb on - strand at 402.902 kb on + strand at 402.902 kb on + strand at 402.902 kb on + strand at 402.902 kb on + strand at 402.902 kb on + strand at 402.902 kb on + strand at 402.903 kb on - strand at 402.903 kb on - strand at 402.903 kb on - strand at 402.903 kb on - strand at 402.903 kb on - strand at 402.903 kb on - strand at 402.903 kb on - strand at 402.903 kb on - strand at 402.903 kb on - strand at 402.903 kb on - strand at 402.903 kb on - strand at 402.903 kb on - strand at 402.903 kb on - strand at 402.903 kb on - strand at 402.903 kb on - strand at 402.903 kb on - strand at 402.903 kb on - strand at 402.903 kb on - strand at 402.986 kb on + strand at 402.986 kb on + strand at 402.986 kb on + strand at 402.986 kb on + strand at 402.987 kb on - strand at 402.987 kb on - strand at 403.022 kb on + strand, within MPMX19_06219 at 403.022 kb on + strand, within MPMX19_06219 at 403.022 kb on + strand, within MPMX19_06219 at 403.022 kb on + strand, within MPMX19_06219 at 403.022 kb on + strand, within MPMX19_06219 at 403.022 kb on + strand, within MPMX19_06219 at 403.022 kb on + strand, within MPMX19_06219 at 403.022 kb on + strand, within MPMX19_06219 at 403.022 kb on + strand, within MPMX19_06219 at 403.022 kb on + strand, within MPMX19_06219 at 403.022 kb on + strand, within MPMX19_06219 at 403.023 kb on - strand, within MPMX19_06219 at 403.023 kb on - strand, within MPMX19_06219 at 403.023 kb on - strand, within MPMX19_06219 at 403.023 kb on - strand, within MPMX19_06219 at 403.023 kb on - strand, within MPMX19_06219 at 403.023 kb on - strand, within MPMX19_06219 at 403.023 kb on - strand, within MPMX19_06219 at 403.023 kb on - strand, within MPMX19_06219 at 403.023 kb on - strand, within MPMX19_06219 at 403.133 kb on + strand, within MPMX19_06219 at 403.133 kb on + strand, within MPMX19_06219 at 403.133 kb on + strand, within MPMX19_06219 at 403.133 kb on + strand, within MPMX19_06219 at 403.133 kb on + strand, within MPMX19_06219 at 403.134 kb on - strand, within MPMX19_06219 at 403.134 kb on - strand, within MPMX19_06219 at 403.134 kb on - strand, within MPMX19_06219 at 403.134 kb on - strand, within MPMX19_06219 at 403.134 kb on - strand, within MPMX19_06219 at 403.134 kb on - strand, within MPMX19_06219 at 403.134 kb on - strand, within MPMX19_06219 at 403.134 kb on - strand, within MPMX19_06219 at 403.202 kb on + strand, within MPMX19_06219 at 403.226 kb on + strand, within MPMX19_06219 at 403.226 kb on + strand, within MPMX19_06219 at 403.226 kb on + strand, within MPMX19_06219 at 403.226 kb on + strand, within MPMX19_06219 at 403.226 kb on + strand, within MPMX19_06219 at 403.226 kb on + strand, within MPMX19_06219 at 403.226 kb on + strand, within MPMX19_06219 at 403.226 kb on + strand, within MPMX19_06219 at 403.226 kb on + strand, within MPMX19_06219 at 403.226 kb on + strand, within MPMX19_06219 at 403.226 kb on + strand, within MPMX19_06219 at 403.226 kb on + strand, within MPMX19_06219 at 403.226 kb on + strand, within MPMX19_06219 at 403.226 kb on + strand, within MPMX19_06219 at 403.226 kb on + strand, within MPMX19_06219 at 403.226 kb on + strand, within MPMX19_06219 at 403.226 kb on + strand, within MPMX19_06219 at 403.227 kb on - strand, within MPMX19_06219 at 403.227 kb on - strand, within MPMX19_06219 at 403.227 kb on - strand, within MPMX19_06219 at 403.227 kb on - strand, within MPMX19_06219 at 403.227 kb on - strand, within MPMX19_06219 at 403.227 kb on - strand, within MPMX19_06219 at 403.227 kb on - strand, within MPMX19_06219 at 403.227 kb on - strand, within MPMX19_06219 at 403.227 kb on - strand, within MPMX19_06219 at 403.227 kb on - strand, within MPMX19_06219 at 403.227 kb on - strand, within MPMX19_06219 at 403.227 kb on - strand, within MPMX19_06219 at 403.227 kb on - strand, within MPMX19_06219 at 403.227 kb on - strand, within MPMX19_06219 at 403.227 kb on - strand, within MPMX19_06219 at 403.227 kb on - strand, within MPMX19_06219 at 403.253 kb on + strand, within MPMX19_06219 at 403.253 kb on + strand, within MPMX19_06219 at 403.254 kb on - strand, within MPMX19_06219 at 403.254 kb on - strand, within MPMX19_06219 at 403.343 kb on + strand, within MPMX19_06219 at 403.343 kb on + strand, within MPMX19_06219 at 403.343 kb on + strand, within MPMX19_06219 at 403.343 kb on + strand, within MPMX19_06219 at 403.343 kb on + strand, within MPMX19_06219 at 403.343 kb on + strand, within MPMX19_06219 at 403.344 kb on - strand, within MPMX19_06219 at 403.344 kb on - strand, within MPMX19_06219 at 403.388 kb on + strand, within MPMX19_06219 at 403.388 kb on + strand, within MPMX19_06219 at 403.388 kb on + strand, within MPMX19_06219 at 403.388 kb on + strand, within MPMX19_06219 at 403.388 kb on + strand, within MPMX19_06219 at 403.389 kb on - strand, within MPMX19_06219 at 403.389 kb on - strand, within MPMX19_06219 at 403.389 kb on - strand, within MPMX19_06219 at 403.389 kb on - strand, within MPMX19_06219 at 403.389 kb on - strand, within MPMX19_06219 at 403.389 kb on - strand, within MPMX19_06219 at 403.389 kb on - strand, within MPMX19_06219 at 403.389 kb on - strand, within MPMX19_06219 at 403.406 kb on + strand, within MPMX19_06219 at 403.406 kb on + strand, within MPMX19_06219 at 403.406 kb on + strand, within MPMX19_06219 at 403.406 kb on + strand, within MPMX19_06219 at 403.406 kb on + strand, within MPMX19_06219 at 403.406 kb on + strand, within MPMX19_06219 at 403.406 kb on + strand, within MPMX19_06219 at 403.406 kb on + strand, within MPMX19_06219 at 403.406 kb on + strand, within MPMX19_06219 at 403.406 kb on + strand, within MPMX19_06219 at 403.406 kb on + strand, within MPMX19_06219 at 403.407 kb on - strand, within MPMX19_06219 at 403.407 kb on - strand, within MPMX19_06219 at 403.407 kb on - strand, within MPMX19_06219 at 403.407 kb on - strand, within MPMX19_06219 at 403.407 kb on - strand, within MPMX19_06219 at 403.407 kb on - strand, within MPMX19_06219 at 403.407 kb on - strand, within MPMX19_06219 at 403.419 kb on - strand, within MPMX19_06219 at 403.473 kb on + strand, within MPMX19_06219 at 403.473 kb on + strand, within MPMX19_06219 at 403.474 kb on - strand, within MPMX19_06219 at 403.474 kb on - strand, within MPMX19_06219 at 403.499 kb on + strand, within MPMX19_06219 at 403.500 kb on - strand, within MPMX19_06219 at 403.500 kb on - strand, within MPMX19_06219 at 403.500 kb on - strand, within MPMX19_06219 at 403.541 kb on + strand, within MPMX19_06219 at 403.541 kb on + strand, within MPMX19_06219 at 403.541 kb on + strand, within MPMX19_06219 at 403.541 kb on + strand, within MPMX19_06219 at 403.542 kb on - strand, within MPMX19_06219 at 403.542 kb on - strand, within MPMX19_06219 at 403.542 kb on - strand, within MPMX19_06219 at 403.542 kb on - strand, within MPMX19_06219 at 403.542 kb on - strand, within MPMX19_06219 at 403.542 kb on - strand, within MPMX19_06219 at 403.542 kb on - strand, within MPMX19_06219 at 403.542 kb on - strand, within MPMX19_06219 at 403.542 kb on - strand, within MPMX19_06219 at 403.542 kb on - strand, within MPMX19_06219 at 403.542 kb on - strand, within MPMX19_06219 at 403.827 kb on - strand, within MPMX19_06219 at 403.928 kb on + strand, within MPMX19_06219 at 403.928 kb on + strand, within MPMX19_06219 at 403.928 kb on + strand, within MPMX19_06219 at 403.928 kb on + strand, within MPMX19_06219 at 403.929 kb on - strand, within MPMX19_06219 at 403.929 kb on - strand, within MPMX19_06219 at 403.929 kb on - strand, within MPMX19_06219 at 403.943 kb on + strand at 404.072 kb on - strand at 404.256 kb on + strand at 404.256 kb on + strand at 404.256 kb on + strand at 404.257 kb on - strand at 404.257 kb on - strand at 405.048 kb on + strand, within MPMX19_06220 at 405.048 kb on + strand, within MPMX19_06220 at 405.048 kb on + strand, within MPMX19_06220 at 405.048 kb on + strand, within MPMX19_06220
Per-strain Table
Position Strand Gene LocusTag Fraction MGL remove 402,061 + MPMX19_06218 0.60 -0.0 402,061 + MPMX19_06218 0.60 +0.2 402,061 + MPMX19_06218 0.60 +0.3 402,061 + MPMX19_06218 0.60 +1.7 402,061 + MPMX19_06218 0.60 -0.4 402,061 + MPMX19_06218 0.60 -0.6 402,061 + MPMX19_06218 0.60 +0.7 402,061 + MPMX19_06218 0.60 +0.2 402,061 + MPMX19_06218 0.60 +0.7 402,061 + MPMX19_06218 0.60 -0.7 402,061 + MPMX19_06218 0.60 -0.8 402,061 + MPMX19_06218 0.60 -0.8 402,061 + MPMX19_06218 0.60 -1.5 402,061 + MPMX19_06218 0.60 +0.4 402,061 + MPMX19_06218 0.60 -0.3 402,061 + MPMX19_06218 0.60 +0.2 402,061 + MPMX19_06218 0.60 -1.0 402,061 + MPMX19_06218 0.60 -0.6 402,062 - MPMX19_06218 0.61 -1.8 402,062 - MPMX19_06218 0.61 -0.9 402,062 - MPMX19_06218 0.61 -1.6 402,062 - MPMX19_06218 0.61 -0.3 402,062 - MPMX19_06218 0.61 -1.0 402,062 - MPMX19_06218 0.61 -1.3 402,062 - MPMX19_06218 0.61 +0.5 402,062 - MPMX19_06218 0.61 -0.3 402,062 - MPMX19_06218 0.61 +0.3 402,062 - MPMX19_06218 0.61 -0.5 402,062 - MPMX19_06218 0.61 -0.4 402,062 - MPMX19_06218 0.61 -0.7 402,062 - MPMX19_06218 0.61 -1.2 402,404 + +0.3 402,464 + +0.8 402,464 + -1.0 402,464 + -0.3 402,476 + -1.0 402,476 + -1.1 402,476 + -1.3 402,476 + -0.1 402,476 + -0.8 402,476 + -1.0 402,476 + -0.4 402,477 - +0.4 402,477 - -2.5 402,477 - -1.6 402,477 - +1.6 402,496 - -1.3 402,509 + -0.1 402,510 - -0.0 402,523 + -0.0 402,523 + +0.6 402,523 + -0.1 402,608 - -1.4 402,667 + -1.0 402,668 - -1.0 402,669 + -0.1 402,669 + -0.3 402,669 + -2.1 402,669 + -1.7 402,669 + -1.4 402,669 + -0.8 402,670 - -1.3 402,670 - -0.0 402,670 - -1.6 402,670 - -0.4 402,670 - +1.0 402,670 - -0.8 402,670 - -0.4 402,902 + -1.3 402,902 + -0.9 402,902 + -0.3 402,902 + -0.8 402,902 + -1.4 402,902 + +0.7 402,903 - -1.1 402,903 - -1.4 402,903 - -3.1 402,903 - -1.4 402,903 - +0.3 402,903 - -0.1 402,903 - -2.5 402,903 - -1.0 402,903 - -1.2 402,903 - -1.8 402,903 - -1.3 402,903 - -2.4 402,903 - +1.0 402,903 - -2.1 402,903 - -0.3 402,903 - -0.0 402,903 - -1.0 402,903 - +0.1 402,986 + -0.3 402,986 + +1.0 402,986 + -1.0 402,986 + +0.3 402,987 - -1.0 402,987 - -1.4 403,022 + MPMX19_06219 0.10 +0.4 403,022 + MPMX19_06219 0.10 +1.3 403,022 + MPMX19_06219 0.10 -1.5 403,022 + MPMX19_06219 0.10 +0.3 403,022 + MPMX19_06219 0.10 -1.0 403,022 + MPMX19_06219 0.10 -1.0 403,022 + MPMX19_06219 0.10 -1.0 403,022 + MPMX19_06219 0.10 +0.3 403,022 + MPMX19_06219 0.10 -0.3 403,022 + MPMX19_06219 0.10 -1.2 403,022 + MPMX19_06219 0.10 -0.0 403,023 - MPMX19_06219 0.11 -0.5 403,023 - MPMX19_06219 0.11 -1.1 403,023 - MPMX19_06219 0.11 -0.8 403,023 - MPMX19_06219 0.11 -1.8 403,023 - MPMX19_06219 0.11 -0.4 403,023 - MPMX19_06219 0.11 +0.2 403,023 - MPMX19_06219 0.11 -0.7 403,023 - MPMX19_06219 0.11 -1.2 403,023 - MPMX19_06219 0.11 -0.8 403,133 + MPMX19_06219 0.20 -0.8 403,133 + MPMX19_06219 0.20 -0.4 403,133 + MPMX19_06219 0.20 -0.0 403,133 + MPMX19_06219 0.20 -0.0 403,133 + MPMX19_06219 0.20 -1.0 403,134 - MPMX19_06219 0.20 -1.3 403,134 - MPMX19_06219 0.20 -1.1 403,134 - MPMX19_06219 0.20 -0.9 403,134 - MPMX19_06219 0.20 -0.8 403,134 - MPMX19_06219 0.20 -0.1 403,134 - MPMX19_06219 0.20 +0.7 403,134 - MPMX19_06219 0.20 -0.0 403,134 - MPMX19_06219 0.20 -1.6 403,202 + MPMX19_06219 0.26 -1.5 403,226 + MPMX19_06219 0.28 -0.3 403,226 + MPMX19_06219 0.28 -0.8 403,226 + MPMX19_06219 0.28 -1.5 403,226 + MPMX19_06219 0.28 -2.1 403,226 + MPMX19_06219 0.28 +0.4 403,226 + MPMX19_06219 0.28 +0.7 403,226 + MPMX19_06219 0.28 -2.1 403,226 + MPMX19_06219 0.28 +0.4 403,226 + MPMX19_06219 0.28 -0.6 403,226 + MPMX19_06219 0.28 -0.5 403,226 + MPMX19_06219 0.28 -1.1 403,226 + MPMX19_06219 0.28 -1.7 403,226 + MPMX19_06219 0.28 +0.3 403,226 + MPMX19_06219 0.28 -0.7 403,226 + MPMX19_06219 0.28 -1.1 403,226 + MPMX19_06219 0.28 -0.0 403,226 + MPMX19_06219 0.28 -0.9 403,227 - MPMX19_06219 0.28 -0.6 403,227 - MPMX19_06219 0.28 -0.8 403,227 - MPMX19_06219 0.28 -1.3 403,227 - MPMX19_06219 0.28 -0.1 403,227 - MPMX19_06219 0.28 +0.3 403,227 - MPMX19_06219 0.28 -0.2 403,227 - MPMX19_06219 0.28 -0.8 403,227 - MPMX19_06219 0.28 +0.1 403,227 - MPMX19_06219 0.28 -0.6 403,227 - MPMX19_06219 0.28 +0.3 403,227 - MPMX19_06219 0.28 +1.3 403,227 - MPMX19_06219 0.28 -0.7 403,227 - MPMX19_06219 0.28 +0.4 403,227 - MPMX19_06219 0.28 -2.0 403,227 - MPMX19_06219 0.28 -0.1 403,227 - MPMX19_06219 0.28 -0.4 403,253 + MPMX19_06219 0.30 -0.6 403,253 + MPMX19_06219 0.30 +0.1 403,254 - MPMX19_06219 0.30 +0.3 403,254 - MPMX19_06219 0.30 -1.0 403,343 + MPMX19_06219 0.38 -1.8 403,343 + MPMX19_06219 0.38 -0.8 403,343 + MPMX19_06219 0.38 +0.7 403,343 + MPMX19_06219 0.38 -1.1 403,343 + MPMX19_06219 0.38 -0.2 403,343 + MPMX19_06219 0.38 -0.1 403,344 - MPMX19_06219 0.38 -0.7 403,344 - MPMX19_06219 0.38 -0.8 403,388 + MPMX19_06219 0.42 -0.7 403,388 + MPMX19_06219 0.42 -1.0 403,388 + MPMX19_06219 0.42 -1.9 403,388 + MPMX19_06219 0.42 -1.4 403,388 + MPMX19_06219 0.42 -1.8 403,389 - MPMX19_06219 0.42 -0.5 403,389 - MPMX19_06219 0.42 -1.8 403,389 - MPMX19_06219 0.42 -1.4 403,389 - MPMX19_06219 0.42 -0.3 403,389 - MPMX19_06219 0.42 -1.2 403,389 - MPMX19_06219 0.42 -3.0 403,389 - MPMX19_06219 0.42 +0.3 403,389 - MPMX19_06219 0.42 -1.2 403,406 + MPMX19_06219 0.44 -0.8 403,406 + MPMX19_06219 0.44 -2.2 403,406 + MPMX19_06219 0.44 -0.2 403,406 + MPMX19_06219 0.44 +1.0 403,406 + MPMX19_06219 0.44 -2.3 403,406 + MPMX19_06219 0.44 -0.2 403,406 + MPMX19_06219 0.44 -1.1 403,406 + MPMX19_06219 0.44 +0.3 403,406 + MPMX19_06219 0.44 -0.8 403,406 + MPMX19_06219 0.44 +0.2 403,406 + MPMX19_06219 0.44 -2.3 403,407 - MPMX19_06219 0.44 -1.1 403,407 - MPMX19_06219 0.44 +1.0 403,407 - MPMX19_06219 0.44 -0.3 403,407 - MPMX19_06219 0.44 -1.5 403,407 - MPMX19_06219 0.44 -0.3 403,407 - MPMX19_06219 0.44 -2.3 403,407 - MPMX19_06219 0.44 -0.5 403,419 - MPMX19_06219 0.45 -1.3 403,473 + MPMX19_06219 0.49 -1.1 403,473 + MPMX19_06219 0.49 -0.4 403,474 - MPMX19_06219 0.49 -0.0 403,474 - MPMX19_06219 0.49 +1.0 403,499 + MPMX19_06219 0.52 -0.3 403,500 - MPMX19_06219 0.52 -0.5 403,500 - MPMX19_06219 0.52 -1.4 403,500 - MPMX19_06219 0.52 -1.3 403,541 + MPMX19_06219 0.55 -0.3 403,541 + MPMX19_06219 0.55 -0.8 403,541 + MPMX19_06219 0.55 +0.2 403,541 + MPMX19_06219 0.55 -0.3 403,542 - MPMX19_06219 0.55 -2.1 403,542 - MPMX19_06219 0.55 -1.1 403,542 - MPMX19_06219 0.55 -0.1 403,542 - MPMX19_06219 0.55 +0.3 403,542 - MPMX19_06219 0.55 -1.4 403,542 - MPMX19_06219 0.55 -1.3 403,542 - MPMX19_06219 0.55 +0.2 403,542 - MPMX19_06219 0.55 +0.1 403,542 - MPMX19_06219 0.55 -0.5 403,542 - MPMX19_06219 0.55 -0.7 403,542 - MPMX19_06219 0.55 +1.7 403,827 - MPMX19_06219 0.80 -1.0 403,928 + MPMX19_06219 0.89 -1.4 403,928 + MPMX19_06219 0.89 -0.1 403,928 + MPMX19_06219 0.89 -1.9 403,928 + MPMX19_06219 0.89 -1.4 403,929 - MPMX19_06219 0.89 +0.1 403,929 - MPMX19_06219 0.89 -1.3 403,929 - MPMX19_06219 0.89 -2.7 403,943 + -1.5 404,072 - -0.4 404,256 + -0.1 404,256 + -0.5 404,256 + -0.8 404,257 - -2.6 404,257 - -1.9 405,048 + MPMX19_06220 0.50 -0.3 405,048 + MPMX19_06220 0.50 -0.9 405,048 + MPMX19_06220 0.50 -0.0 405,048 + MPMX19_06220 0.50 -0.7
Or see this region's nucleotide sequence