Experiment: MGL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX19_02794 and MPMX19_02795 are separated by 26 nucleotides MPMX19_02795 and MPMX19_02796 are separated by 8 nucleotides MPMX19_02796 and MPMX19_02797 overlap by 17 nucleotides
MPMX19_02794: MPMX19_02794 - hypothetical protein, at 28,250 to 29,839
_02794
MPMX19_02795: MPMX19_02795 - Homoaconitase large subunit, at 29,866 to 31,122
_02795
MPMX19_02796: MPMX19_02796 - Methanogen homoaconitase small subunit, at 31,131 to 31,694
_02796
MPMX19_02797: MPMX19_02797 - 2,3-dimethylmalate lyase, at 31,678 to 32,538
_02797
Position (kb) 29
30
31
32 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 29.495 kb on + strand, within MPMX19_02794 at 29.495 kb on + strand, within MPMX19_02794 at 29.495 kb on + strand, within MPMX19_02794 at 29.495 kb on + strand, within MPMX19_02794 at 29.496 kb on - strand, within MPMX19_02794 at 29.496 kb on - strand, within MPMX19_02794 at 29.496 kb on - strand, within MPMX19_02794 at 29.496 kb on - strand, within MPMX19_02794 at 29.549 kb on + strand, within MPMX19_02794 at 29.549 kb on + strand, within MPMX19_02794 at 29.549 kb on + strand, within MPMX19_02794 at 29.550 kb on - strand, within MPMX19_02794 at 29.550 kb on - strand, within MPMX19_02794 at 29.684 kb on + strand at 29.684 kb on + strand at 29.684 kb on + strand at 29.684 kb on + strand at 29.684 kb on + strand at 29.684 kb on + strand at 29.685 kb on - strand at 29.685 kb on - strand at 29.685 kb on - strand at 29.685 kb on - strand at 29.685 kb on - strand at 29.685 kb on - strand at 29.727 kb on - strand at 29.872 kb on + strand at 29.872 kb on + strand at 29.872 kb on + strand at 29.872 kb on + strand at 29.873 kb on - strand at 29.873 kb on - strand at 29.968 kb on + strand at 29.968 kb on + strand at 29.969 kb on - strand at 29.995 kb on + strand, within MPMX19_02795 at 29.995 kb on + strand, within MPMX19_02795 at 29.996 kb on - strand, within MPMX19_02795 at 29.996 kb on - strand, within MPMX19_02795 at 29.996 kb on - strand, within MPMX19_02795 at 30.072 kb on + strand, within MPMX19_02795 at 30.072 kb on + strand, within MPMX19_02795 at 30.072 kb on + strand, within MPMX19_02795 at 30.072 kb on + strand, within MPMX19_02795 at 30.072 kb on + strand, within MPMX19_02795 at 30.073 kb on - strand, within MPMX19_02795 at 30.073 kb on - strand, within MPMX19_02795 at 30.073 kb on - strand, within MPMX19_02795 at 30.073 kb on - strand, within MPMX19_02795 at 30.073 kb on - strand, within MPMX19_02795 at 30.073 kb on - strand, within MPMX19_02795 at 30.073 kb on - strand, within MPMX19_02795 at 30.199 kb on + strand, within MPMX19_02795 at 30.200 kb on - strand, within MPMX19_02795 at 30.200 kb on - strand, within MPMX19_02795 at 30.571 kb on + strand, within MPMX19_02795 at 30.571 kb on + strand, within MPMX19_02795 at 30.571 kb on + strand, within MPMX19_02795 at 30.572 kb on - strand, within MPMX19_02795 at 30.572 kb on - strand, within MPMX19_02795 at 30.572 kb on - strand, within MPMX19_02795 at 30.631 kb on + strand, within MPMX19_02795 at 30.632 kb on - strand, within MPMX19_02795 at 30.632 kb on - strand, within MPMX19_02795 at 30.632 kb on - strand, within MPMX19_02795 at 30.748 kb on + strand, within MPMX19_02795 at 30.946 kb on + strand, within MPMX19_02795 at 30.947 kb on - strand, within MPMX19_02795 at 30.947 kb on - strand, within MPMX19_02795 at 31.048 kb on + strand at 31.049 kb on - strand at 31.049 kb on - strand at 31.049 kb on - strand at 31.049 kb on - strand at 31.049 kb on - strand at 31.049 kb on - strand at 31.115 kb on - strand at 31.115 kb on - strand at 31.115 kb on - strand at 31.115 kb on - strand at 31.148 kb on + strand at 31.148 kb on + strand at 31.148 kb on + strand at 31.148 kb on + strand at 31.148 kb on + strand at 31.148 kb on + strand at 31.148 kb on + strand at 31.148 kb on + strand at 31.148 kb on + strand at 31.148 kb on + strand at 31.148 kb on + strand at 31.148 kb on + strand at 31.148 kb on + strand at 31.148 kb on + strand at 31.148 kb on + strand at 31.148 kb on + strand at 31.148 kb on + strand at 31.148 kb on + strand at 31.148 kb on + strand at 31.149 kb on - strand at 31.149 kb on - strand at 31.149 kb on - strand at 31.149 kb on - strand at 31.149 kb on - strand at 31.149 kb on - strand at 31.149 kb on - strand at 31.149 kb on - strand at 31.149 kb on - strand at 31.149 kb on - strand at 31.149 kb on - strand at 31.149 kb on - strand at 31.149 kb on - strand at 31.149 kb on - strand at 31.149 kb on - strand at 31.149 kb on - strand at 31.149 kb on - strand at 31.149 kb on - strand at 31.149 kb on - strand at 31.149 kb on - strand at 31.149 kb on - strand at 31.151 kb on + strand at 31.151 kb on + strand at 31.151 kb on + strand at 31.152 kb on - strand at 31.152 kb on - strand at 31.152 kb on - strand at 31.152 kb on - strand at 31.152 kb on - strand at 31.152 kb on - strand at 31.281 kb on + strand, within MPMX19_02796 at 31.281 kb on + strand, within MPMX19_02796 at 31.281 kb on + strand, within MPMX19_02796 at 31.281 kb on + strand, within MPMX19_02796 at 31.281 kb on + strand, within MPMX19_02796 at 31.281 kb on + strand, within MPMX19_02796 at 31.281 kb on + strand, within MPMX19_02796 at 31.281 kb on + strand, within MPMX19_02796 at 31.282 kb on - strand, within MPMX19_02796 at 31.282 kb on - strand, within MPMX19_02796 at 31.282 kb on - strand, within MPMX19_02796 at 31.282 kb on - strand, within MPMX19_02796 at 31.282 kb on - strand, within MPMX19_02796 at 31.282 kb on - strand, within MPMX19_02796 at 31.362 kb on + strand, within MPMX19_02796 at 31.744 kb on + strand at 31.744 kb on + strand at 31.745 kb on - strand at 31.745 kb on - strand at 31.745 kb on - strand at 31.745 kb on - strand at 31.745 kb on - strand at 31.852 kb on + strand, within MPMX19_02797 at 31.852 kb on + strand, within MPMX19_02797 at 31.852 kb on + strand, within MPMX19_02797 at 31.852 kb on + strand, within MPMX19_02797 at 31.852 kb on + strand, within MPMX19_02797 at 31.852 kb on + strand, within MPMX19_02797 at 31.852 kb on + strand, within MPMX19_02797 at 31.852 kb on + strand, within MPMX19_02797 at 31.852 kb on + strand, within MPMX19_02797 at 31.852 kb on + strand, within MPMX19_02797 at 31.852 kb on + strand, within MPMX19_02797 at 31.853 kb on - strand, within MPMX19_02797 at 31.853 kb on - strand, within MPMX19_02797 at 31.853 kb on - strand, within MPMX19_02797 at 31.853 kb on - strand, within MPMX19_02797 at 31.853 kb on - strand, within MPMX19_02797 at 31.853 kb on - strand, within MPMX19_02797 at 31.853 kb on - strand, within MPMX19_02797 at 31.853 kb on - strand, within MPMX19_02797 at 31.853 kb on - strand, within MPMX19_02797
Per-strain Table
Position Strand Gene LocusTag Fraction MGL remove 29,495 + MPMX19_02794 0.78 -1.4 29,495 + MPMX19_02794 0.78 -1.1 29,495 + MPMX19_02794 0.78 -0.1 29,495 + MPMX19_02794 0.78 -0.5 29,496 - MPMX19_02794 0.78 -0.6 29,496 - MPMX19_02794 0.78 +0.8 29,496 - MPMX19_02794 0.78 -0.1 29,496 - MPMX19_02794 0.78 +1.6 29,549 + MPMX19_02794 0.82 -0.1 29,549 + MPMX19_02794 0.82 +0.2 29,549 + MPMX19_02794 0.82 -0.6 29,550 - MPMX19_02794 0.82 -1.9 29,550 - MPMX19_02794 0.82 -1.1 29,684 + -0.3 29,684 + -2.1 29,684 + -1.4 29,684 + -1.9 29,684 + -0.6 29,684 + -0.3 29,685 - -1.4 29,685 - -0.2 29,685 - -1.7 29,685 - +1.2 29,685 - +0.6 29,685 - -0.1 29,727 - -1.4 29,872 + -0.5 29,872 + -1.1 29,872 + -0.9 29,872 + +0.4 29,873 - +0.2 29,873 - -0.4 29,968 + +0.6 29,968 + +0.6 29,969 - +0.2 29,995 + MPMX19_02795 0.10 -1.0 29,995 + MPMX19_02795 0.10 +0.2 29,996 - MPMX19_02795 0.10 -1.3 29,996 - MPMX19_02795 0.10 -0.1 29,996 - MPMX19_02795 0.10 -1.1 30,072 + MPMX19_02795 0.16 -0.8 30,072 + MPMX19_02795 0.16 +0.8 30,072 + MPMX19_02795 0.16 -0.4 30,072 + MPMX19_02795 0.16 -1.4 30,072 + MPMX19_02795 0.16 -0.4 30,073 - MPMX19_02795 0.16 -0.2 30,073 - MPMX19_02795 0.16 -1.3 30,073 - MPMX19_02795 0.16 +0.9 30,073 - MPMX19_02795 0.16 -2.5 30,073 - MPMX19_02795 0.16 -1.1 30,073 - MPMX19_02795 0.16 +0.0 30,073 - MPMX19_02795 0.16 -1.4 30,199 + MPMX19_02795 0.26 -1.9 30,200 - MPMX19_02795 0.27 +0.2 30,200 - MPMX19_02795 0.27 +0.0 30,571 + MPMX19_02795 0.56 +0.8 30,571 + MPMX19_02795 0.56 -0.2 30,571 + MPMX19_02795 0.56 -1.0 30,572 - MPMX19_02795 0.56 -0.4 30,572 - MPMX19_02795 0.56 -0.4 30,572 - MPMX19_02795 0.56 -0.5 30,631 + MPMX19_02795 0.61 -2.4 30,632 - MPMX19_02795 0.61 -0.7 30,632 - MPMX19_02795 0.61 -1.2 30,632 - MPMX19_02795 0.61 -1.8 30,748 + MPMX19_02795 0.70 -0.2 30,946 + MPMX19_02795 0.86 -1.2 30,947 - MPMX19_02795 0.86 +0.4 30,947 - MPMX19_02795 0.86 -0.2 31,048 + -1.1 31,049 - -0.7 31,049 - -2.9 31,049 - -2.0 31,049 - -0.1 31,049 - -1.8 31,049 - -0.4 31,115 - -1.0 31,115 - -0.7 31,115 - -1.7 31,115 - -1.1 31,148 + +0.2 31,148 + -2.2 31,148 + -0.3 31,148 + -1.6 31,148 + -1.6 31,148 + +0.0 31,148 + +0.1 31,148 + +0.9 31,148 + -2.5 31,148 + -0.4 31,148 + +0.4 31,148 + -1.8 31,148 + +1.2 31,148 + +0.0 31,148 + -0.8 31,148 + +1.0 31,148 + +0.0 31,148 + -1.0 31,148 + -0.8 31,149 - -0.2 31,149 - -1.5 31,149 - +0.4 31,149 - -0.1 31,149 - -2.2 31,149 - +0.0 31,149 - -1.5 31,149 - -0.6 31,149 - +0.0 31,149 - -0.4 31,149 - -0.4 31,149 - -0.4 31,149 - -1.0 31,149 - -2.2 31,149 - -1.9 31,149 - -0.4 31,149 - -1.4 31,149 - -0.2 31,149 - -1.1 31,149 - -1.3 31,149 - -1.2 31,151 + -1.9 31,151 + -1.2 31,151 + -1.0 31,152 - -1.6 31,152 - -1.1 31,152 - -1.3 31,152 - +0.8 31,152 - -1.0 31,152 - -1.4 31,281 + MPMX19_02796 0.27 -0.1 31,281 + MPMX19_02796 0.27 -0.4 31,281 + MPMX19_02796 0.27 +1.6 31,281 + MPMX19_02796 0.27 -1.0 31,281 + MPMX19_02796 0.27 -0.4 31,281 + MPMX19_02796 0.27 -0.6 31,281 + MPMX19_02796 0.27 -2.3 31,281 + MPMX19_02796 0.27 -1.8 31,282 - MPMX19_02796 0.27 -1.2 31,282 - MPMX19_02796 0.27 -0.6 31,282 - MPMX19_02796 0.27 -0.3 31,282 - MPMX19_02796 0.27 -1.3 31,282 - MPMX19_02796 0.27 -1.8 31,282 - MPMX19_02796 0.27 -2.2 31,362 + MPMX19_02796 0.41 +0.4 31,744 + -2.0 31,744 + -0.6 31,745 - +0.5 31,745 - -0.6 31,745 - -0.3 31,745 - -0.2 31,745 - -0.1 31,852 + MPMX19_02797 0.20 -1.4 31,852 + MPMX19_02797 0.20 -0.2 31,852 + MPMX19_02797 0.20 +0.8 31,852 + MPMX19_02797 0.20 -0.4 31,852 + MPMX19_02797 0.20 -0.3 31,852 + MPMX19_02797 0.20 +1.4 31,852 + MPMX19_02797 0.20 -0.3 31,852 + MPMX19_02797 0.20 -0.8 31,852 + MPMX19_02797 0.20 +0.0 31,852 + MPMX19_02797 0.20 -1.0 31,852 + MPMX19_02797 0.20 -0.6 31,853 - MPMX19_02797 0.20 -0.4 31,853 - MPMX19_02797 0.20 -1.3 31,853 - MPMX19_02797 0.20 -1.7 31,853 - MPMX19_02797 0.20 -1.8 31,853 - MPMX19_02797 0.20 -0.1 31,853 - MPMX19_02797 0.20 -0.1 31,853 - MPMX19_02797 0.20 +1.1 31,853 - MPMX19_02797 0.20 -1.9 31,853 - MPMX19_02797 0.20 -1.0
Or see this region's nucleotide sequence