Strain Fitness in Escherichia coli HS(pFamp)R (ATCC 700891) around OHPLBJKB_00949

Experiment: lib only

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOHPLBJKB_00947 and OHPLBJKB_00948 are separated by 77 nucleotidesOHPLBJKB_00948 and OHPLBJKB_00949 overlap by 1 nucleotidesOHPLBJKB_00949 and OHPLBJKB_00950 are separated by 70 nucleotides OHPLBJKB_00947: OHPLBJKB_00947 - GTP pyrophosphokinase, at 979,041 to 981,275 _00947 OHPLBJKB_00948: OHPLBJKB_00948 - Antitoxin MazE, at 981,353 to 981,601 _00948 OHPLBJKB_00949: OHPLBJKB_00949 - Endoribonuclease toxin MazF, at 981,601 to 981,936 _00949 OHPLBJKB_00950: OHPLBJKB_00950 - Nucleoside triphosphate pyrophosphohydrolase, at 982,007 to 982,798 _00950 Position (kb) 981 982Strain fitness (log2 ratio) -2 -1 0 1at 980.732 kb on + strand, within OHPLBJKB_00947at 980.732 kb on + strand, within OHPLBJKB_00947at 980.732 kb on + strand, within OHPLBJKB_00947at 980.733 kb on - strand, within OHPLBJKB_00947at 980.736 kb on - strand, within OHPLBJKB_00947at 980.793 kb on + strand, within OHPLBJKB_00947at 980.793 kb on + strand, within OHPLBJKB_00947at 980.793 kb on + strand, within OHPLBJKB_00947at 980.813 kb on + strand, within OHPLBJKB_00947at 980.833 kb on + strand, within OHPLBJKB_00947at 980.939 kb on - strand, within OHPLBJKB_00947at 981.021 kb on + strand, within OHPLBJKB_00947at 981.069 kb on - strandat 981.079 kb on + strandat 981.080 kb on - strandat 981.080 kb on - strandat 981.190 kb on - strandat 981.221 kb on + strandat 981.273 kb on + strandat 981.295 kb on + strandat 981.315 kb on - strandat 981.352 kb on + strandat 981.352 kb on + strandat 981.352 kb on + strandat 981.353 kb on - strandat 981.364 kb on + strandat 981.437 kb on - strand, within OHPLBJKB_00948at 981.477 kb on + strand, within OHPLBJKB_00948at 981.478 kb on - strand, within OHPLBJKB_00948at 981.481 kb on + strand, within OHPLBJKB_00948at 981.482 kb on - strand, within OHPLBJKB_00948at 981.511 kb on + strand, within OHPLBJKB_00948at 981.512 kb on - strand, within OHPLBJKB_00948at 981.586 kb on + strandat 981.586 kb on + strandat 981.586 kb on + strandat 981.599 kb on + strandat 981.600 kb on - strandat 981.605 kb on + strandat 981.605 kb on + strandat 981.606 kb on - strandat 981.613 kb on + strandat 981.614 kb on - strandat 981.614 kb on - strandat 981.716 kb on - strand, within OHPLBJKB_00949at 981.716 kb on - strand, within OHPLBJKB_00949at 981.716 kb on - strand, within OHPLBJKB_00949at 981.716 kb on - strand, within OHPLBJKB_00949at 981.716 kb on - strand, within OHPLBJKB_00949at 981.716 kb on - strand, within OHPLBJKB_00949at 981.716 kb on - strand, within OHPLBJKB_00949at 981.732 kb on + strand, within OHPLBJKB_00949at 981.732 kb on + strand, within OHPLBJKB_00949at 981.733 kb on - strand, within OHPLBJKB_00949at 981.733 kb on - strand, within OHPLBJKB_00949at 981.733 kb on - strand, within OHPLBJKB_00949at 981.753 kb on + strand, within OHPLBJKB_00949at 981.840 kb on + strand, within OHPLBJKB_00949at 981.840 kb on + strand, within OHPLBJKB_00949at 981.896 kb on + strand, within OHPLBJKB_00949at 981.896 kb on + strand, within OHPLBJKB_00949at 981.896 kb on + strand, within OHPLBJKB_00949at 981.896 kb on + strand, within OHPLBJKB_00949at 981.897 kb on - strand, within OHPLBJKB_00949at 981.897 kb on - strand, within OHPLBJKB_00949at 981.907 kb on - strandat 981.907 kb on - strandat 981.923 kb on + strandat 981.940 kb on + strandat 981.940 kb on + strandat 981.943 kb on + strandat 981.943 kb on + strandat 981.944 kb on - strandat 981.944 kb on - strandat 982.033 kb on + strandat 982.034 kb on - strandat 982.034 kb on - strandat 982.034 kb on - strandat 982.036 kb on + strandat 982.036 kb on + strandat 982.036 kb on + strandat 982.036 kb on + strandat 982.036 kb on + strandat 982.037 kb on - strandat 982.037 kb on - strandat 982.110 kb on - strand, within OHPLBJKB_00950at 982.110 kb on - strand, within OHPLBJKB_00950at 982.127 kb on + strand, within OHPLBJKB_00950at 982.128 kb on - strand, within OHPLBJKB_00950at 982.128 kb on - strand, within OHPLBJKB_00950at 982.212 kb on - strand, within OHPLBJKB_00950at 982.249 kb on + strand, within OHPLBJKB_00950at 982.250 kb on - strand, within OHPLBJKB_00950at 982.255 kb on + strand, within OHPLBJKB_00950at 982.255 kb on + strand, within OHPLBJKB_00950at 982.267 kb on + strand, within OHPLBJKB_00950at 982.267 kb on + strand, within OHPLBJKB_00950at 982.271 kb on - strand, within OHPLBJKB_00950at 982.312 kb on + strand, within OHPLBJKB_00950at 982.312 kb on + strand, within OHPLBJKB_00950at 982.417 kb on + strand, within OHPLBJKB_00950at 982.418 kb on - strand, within OHPLBJKB_00950at 982.431 kb on + strand, within OHPLBJKB_00950at 982.514 kb on + strand, within OHPLBJKB_00950at 982.538 kb on + strand, within OHPLBJKB_00950at 982.538 kb on + strand, within OHPLBJKB_00950at 982.538 kb on + strand, within OHPLBJKB_00950at 982.538 kb on + strand, within OHPLBJKB_00950at 982.538 kb on + strand, within OHPLBJKB_00950at 982.538 kb on + strand, within OHPLBJKB_00950at 982.539 kb on - strand, within OHPLBJKB_00950at 982.539 kb on - strand, within OHPLBJKB_00950at 982.539 kb on - strand, within OHPLBJKB_00950at 982.539 kb on - strand, within OHPLBJKB_00950at 982.539 kb on - strand, within OHPLBJKB_00950at 982.539 kb on - strand, within OHPLBJKB_00950at 982.539 kb on - strand, within OHPLBJKB_00950at 982.570 kb on + strand, within OHPLBJKB_00950at 982.702 kb on + strand, within OHPLBJKB_00950at 982.703 kb on - strand, within OHPLBJKB_00950at 982.703 kb on - strand, within OHPLBJKB_00950at 982.774 kb on - strandat 982.886 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction lib only
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980,732 + OHPLBJKB_00947 0.76 +0.3
980,732 + OHPLBJKB_00947 0.76 +0.2
980,732 + OHPLBJKB_00947 0.76 -0.3
980,733 - OHPLBJKB_00947 0.76 +1.1
980,736 - OHPLBJKB_00947 0.76 -0.4
980,793 + OHPLBJKB_00947 0.78 -0.9
980,793 + OHPLBJKB_00947 0.78 +0.4
980,793 + OHPLBJKB_00947 0.78 -0.9
980,813 + OHPLBJKB_00947 0.79 +1.1
980,833 + OHPLBJKB_00947 0.80 +0.3
980,939 - OHPLBJKB_00947 0.85 +0.0
981,021 + OHPLBJKB_00947 0.89 -0.2
981,069 - -0.2
981,079 + -0.1
981,080 - -0.9
981,080 - -0.7
981,190 - -0.3
981,221 + +0.2
981,273 + -0.1
981,295 + +0.6
981,315 - -0.4
981,352 + -1.3
981,352 + -0.1
981,352 + +0.3
981,353 - +0.9
981,364 + +0.3
981,437 - OHPLBJKB_00948 0.34 -0.7
981,477 + OHPLBJKB_00948 0.50 +0.1
981,478 - OHPLBJKB_00948 0.50 -0.2
981,481 + OHPLBJKB_00948 0.51 +0.2
981,482 - OHPLBJKB_00948 0.52 -0.0
981,511 + OHPLBJKB_00948 0.63 +0.3
981,512 - OHPLBJKB_00948 0.64 +0.1
981,586 + -1.6
981,586 + -0.8
981,586 + -0.3
981,599 + +0.2
981,600 - -0.7
981,605 + -1.0
981,605 + +0.4
981,606 - +1.6
981,613 + -0.1
981,614 - -0.9
981,614 - +0.5
981,716 - OHPLBJKB_00949 0.34 +0.5
981,716 - OHPLBJKB_00949 0.34 -0.3
981,716 - OHPLBJKB_00949 0.34 -0.6
981,716 - OHPLBJKB_00949 0.34 +0.1
981,716 - OHPLBJKB_00949 0.34 -1.2
981,716 - OHPLBJKB_00949 0.34 +0.0
981,716 - OHPLBJKB_00949 0.34 -0.1
981,732 + OHPLBJKB_00949 0.39 -2.2
981,732 + OHPLBJKB_00949 0.39 -0.2
981,733 - OHPLBJKB_00949 0.39 -1.5
981,733 - OHPLBJKB_00949 0.39 -0.0
981,733 - OHPLBJKB_00949 0.39 -0.2
981,753 + OHPLBJKB_00949 0.45 -0.4
981,840 + OHPLBJKB_00949 0.71 -0.9
981,840 + OHPLBJKB_00949 0.71 -0.7
981,896 + OHPLBJKB_00949 0.88 -0.4
981,896 + OHPLBJKB_00949 0.88 +0.1
981,896 + OHPLBJKB_00949 0.88 -0.6
981,896 + OHPLBJKB_00949 0.88 -0.8
981,897 - OHPLBJKB_00949 0.88 +0.3
981,897 - OHPLBJKB_00949 0.88 +0.4
981,907 - +0.5
981,907 - +0.0
981,923 + +0.7
981,940 + +0.4
981,940 + +0.7
981,943 + +1.2
981,943 + -0.4
981,944 - +0.2
981,944 - -2.7
982,033 + -0.1
982,034 - +0.4
982,034 - -0.0
982,034 - -2.1
982,036 + -1.2
982,036 + -0.1
982,036 + -0.0
982,036 + +0.2
982,036 + +0.4
982,037 - +0.0
982,037 - +0.0
982,110 - OHPLBJKB_00950 0.13 +0.2
982,110 - OHPLBJKB_00950 0.13 -0.4
982,127 + OHPLBJKB_00950 0.15 -1.0
982,128 - OHPLBJKB_00950 0.15 -0.3
982,128 - OHPLBJKB_00950 0.15 -1.1
982,212 - OHPLBJKB_00950 0.26 +0.1
982,249 + OHPLBJKB_00950 0.31 +0.6
982,250 - OHPLBJKB_00950 0.31 -0.8
982,255 + OHPLBJKB_00950 0.31 +0.6
982,255 + OHPLBJKB_00950 0.31 -0.8
982,267 + OHPLBJKB_00950 0.33 -0.5
982,267 + OHPLBJKB_00950 0.33 -1.1
982,271 - OHPLBJKB_00950 0.33 -0.1
982,312 + OHPLBJKB_00950 0.39 +0.3
982,312 + OHPLBJKB_00950 0.39 -0.3
982,417 + OHPLBJKB_00950 0.52 -0.4
982,418 - OHPLBJKB_00950 0.52 -0.3
982,431 + OHPLBJKB_00950 0.54 +0.4
982,514 + OHPLBJKB_00950 0.64 -1.0
982,538 + OHPLBJKB_00950 0.67 -0.4
982,538 + OHPLBJKB_00950 0.67 -0.1
982,538 + OHPLBJKB_00950 0.67 +0.0
982,538 + OHPLBJKB_00950 0.67 +0.2
982,538 + OHPLBJKB_00950 0.67 -0.8
982,538 + OHPLBJKB_00950 0.67 -0.2
982,539 - OHPLBJKB_00950 0.67 +0.0
982,539 - OHPLBJKB_00950 0.67 +0.3
982,539 - OHPLBJKB_00950 0.67 -1.9
982,539 - OHPLBJKB_00950 0.67 -0.4
982,539 - OHPLBJKB_00950 0.67 -0.6
982,539 - OHPLBJKB_00950 0.67 -1.1
982,539 - OHPLBJKB_00950 0.67 -0.5
982,570 + OHPLBJKB_00950 0.71 +0.5
982,702 + OHPLBJKB_00950 0.88 -0.1
982,703 - OHPLBJKB_00950 0.88 +0.0
982,703 - OHPLBJKB_00950 0.88 -0.6
982,774 - +1.6
982,886 - -0.4

Or see this region's nucleotide sequence