Experiment: Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF839 and GFF840 are separated by 4 nucleotides GFF840 and GFF841 are separated by 245 nucleotides GFF841 and GFF842 are separated by 84 nucleotides GFF842 and GFF843 overlap by 11 nucleotides
GFF839 - Nicotinamide-nucleotide amidohydrolase PncC, at 77,423 to 77,923
GFF839
GFF840 - hypothetical protein, at 77,928 to 78,290
GFF840
GFF841 - Protein RecA, at 78,536 to 79,564
GFF841
GFF842 - Regulatory protein RecX, at 79,649 to 80,104
GFF842
GFF843 - Alanine--tRNA ligase, at 80,094 to 82,730
GFF843
Position (kb) 78
79
80 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 77.582 kb on + strand, within GFF839 at 77.583 kb on - strand, within GFF839 at 77.583 kb on - strand, within GFF839 at 77.583 kb on - strand, within GFF839 at 77.688 kb on + strand, within GFF839 at 77.707 kb on + strand, within GFF839 at 77.722 kb on - strand, within GFF839 at 77.756 kb on + strand, within GFF839 at 77.756 kb on + strand, within GFF839 at 77.757 kb on - strand, within GFF839 at 77.757 kb on - strand, within GFF839 at 77.757 kb on - strand, within GFF839 at 77.757 kb on - strand, within GFF839 at 77.757 kb on - strand, within GFF839 at 77.757 kb on - strand, within GFF839 at 77.757 kb on - strand, within GFF839 at 77.757 kb on - strand, within GFF839 at 77.757 kb on - strand, within GFF839 at 77.775 kb on + strand, within GFF839 at 77.775 kb on + strand, within GFF839 at 77.776 kb on - strand, within GFF839 at 77.776 kb on - strand, within GFF839 at 77.839 kb on + strand, within GFF839 at 77.859 kb on + strand, within GFF839 at 77.859 kb on + strand, within GFF839 at 77.859 kb on + strand, within GFF839 at 77.859 kb on + strand, within GFF839 at 77.859 kb on + strand, within GFF839 at 77.860 kb on - strand, within GFF839 at 77.860 kb on - strand, within GFF839 at 77.860 kb on - strand, within GFF839 at 77.879 kb on + strand at 77.880 kb on - strand at 77.880 kb on - strand at 77.883 kb on + strand at 77.883 kb on + strand at 77.884 kb on - strand at 77.898 kb on + strand at 77.898 kb on + strand at 77.898 kb on + strand at 77.899 kb on - strand at 77.899 kb on - strand at 77.899 kb on - strand at 77.899 kb on - strand at 77.902 kb on + strand at 77.902 kb on + strand at 77.902 kb on + strand at 77.930 kb on - strand at 77.930 kb on - strand at 77.930 kb on - strand at 77.980 kb on + strand, within GFF840 at 77.980 kb on + strand, within GFF840 at 77.980 kb on + strand, within GFF840 at 77.980 kb on + strand, within GFF840 at 77.980 kb on + strand, within GFF840 at 77.981 kb on - strand, within GFF840 at 77.981 kb on - strand, within GFF840 at 77.981 kb on - strand, within GFF840 at 77.981 kb on - strand, within GFF840 at 77.981 kb on - strand, within GFF840 at 77.983 kb on + strand, within GFF840 at 77.983 kb on + strand, within GFF840 at 77.983 kb on + strand, within GFF840 at 77.983 kb on + strand, within GFF840 at 77.983 kb on + strand, within GFF840 at 77.983 kb on + strand, within GFF840 at 77.983 kb on + strand, within GFF840 at 77.983 kb on + strand, within GFF840 at 77.983 kb on + strand, within GFF840 at 77.983 kb on + strand, within GFF840 at 77.983 kb on + strand, within GFF840 at 77.983 kb on + strand, within GFF840 at 77.984 kb on - strand, within GFF840 at 77.984 kb on - strand, within GFF840 at 77.984 kb on - strand, within GFF840 at 77.984 kb on - strand, within GFF840 at 77.984 kb on - strand, within GFF840 at 77.984 kb on - strand, within GFF840 at 77.998 kb on + strand, within GFF840 at 77.998 kb on + strand, within GFF840 at 77.998 kb on + strand, within GFF840 at 77.998 kb on + strand, within GFF840 at 77.998 kb on + strand, within GFF840 at 77.998 kb on + strand, within GFF840 at 77.999 kb on - strand, within GFF840 at 77.999 kb on - strand, within GFF840 at 77.999 kb on - strand, within GFF840 at 77.999 kb on - strand, within GFF840 at 77.999 kb on - strand, within GFF840 at 77.999 kb on - strand, within GFF840 at 77.999 kb on - strand, within GFF840 at 78.005 kb on - strand, within GFF840 at 78.029 kb on - strand, within GFF840 at 78.041 kb on + strand, within GFF840 at 78.042 kb on - strand, within GFF840 at 78.042 kb on - strand, within GFF840 at 78.076 kb on + strand, within GFF840 at 78.076 kb on + strand, within GFF840 at 78.095 kb on + strand, within GFF840 at 78.095 kb on + strand, within GFF840 at 78.095 kb on + strand, within GFF840 at 78.095 kb on + strand, within GFF840 at 78.096 kb on - strand, within GFF840 at 78.096 kb on - strand, within GFF840 at 78.097 kb on + strand, within GFF840 at 78.097 kb on + strand, within GFF840 at 78.097 kb on + strand, within GFF840 at 78.097 kb on + strand, within GFF840 at 78.097 kb on + strand, within GFF840 at 78.097 kb on + strand, within GFF840 at 78.098 kb on - strand, within GFF840 at 78.098 kb on - strand, within GFF840 at 78.098 kb on - strand, within GFF840 at 78.098 kb on - strand, within GFF840 at 78.098 kb on - strand, within GFF840 at 78.100 kb on + strand, within GFF840 at 78.100 kb on + strand, within GFF840 at 78.101 kb on - strand, within GFF840 at 78.101 kb on - strand, within GFF840 at 78.101 kb on - strand, within GFF840 at 78.101 kb on - strand, within GFF840 at 78.101 kb on - strand, within GFF840 at 78.101 kb on - strand, within GFF840 at 78.101 kb on - strand, within GFF840 at 78.101 kb on - strand, within GFF840 at 78.101 kb on - strand, within GFF840 at 78.101 kb on - strand, within GFF840 at 78.103 kb on + strand, within GFF840 at 78.148 kb on + strand, within GFF840 at 78.148 kb on + strand, within GFF840 at 78.169 kb on + strand, within GFF840 at 78.172 kb on + strand, within GFF840 at 78.173 kb on - strand, within GFF840 at 78.173 kb on - strand, within GFF840 at 78.173 kb on - strand, within GFF840 at 78.196 kb on - strand, within GFF840 at 78.218 kb on + strand, within GFF840 at 78.218 kb on + strand, within GFF840 at 78.218 kb on + strand, within GFF840 at 78.218 kb on + strand, within GFF840 at 78.219 kb on - strand, within GFF840 at 78.219 kb on - strand, within GFF840 at 78.219 kb on - strand, within GFF840 at 78.279 kb on - strand at 78.290 kb on + strand at 78.290 kb on + strand at 78.290 kb on + strand at 78.290 kb on + strand at 78.291 kb on - strand at 78.291 kb on - strand at 78.291 kb on - strand at 78.291 kb on - strand at 78.332 kb on + strand at 78.332 kb on + strand at 78.332 kb on + strand at 78.332 kb on + strand at 78.332 kb on + strand at 78.333 kb on - strand at 78.333 kb on - strand at 78.333 kb on - strand at 78.333 kb on - strand at 78.333 kb on - strand at 78.333 kb on - strand at 78.333 kb on - strand at 78.335 kb on + strand at 78.418 kb on + strand at 78.418 kb on + strand at 78.419 kb on - strand at 78.419 kb on - strand at 78.471 kb on + strand at 78.483 kb on + strand at 79.187 kb on + strand, within GFF841 at 79.300 kb on + strand, within GFF841 at 79.321 kb on + strand, within GFF841 at 79.321 kb on + strand, within GFF841 at 79.321 kb on + strand, within GFF841 at 79.321 kb on + strand, within GFF841 at 79.321 kb on + strand, within GFF841 at 79.321 kb on + strand, within GFF841 at 79.321 kb on + strand, within GFF841 at 79.321 kb on + strand, within GFF841 at 79.325 kb on + strand, within GFF841 at 79.408 kb on + strand, within GFF841 at 79.412 kb on + strand, within GFF841 at 79.412 kb on + strand, within GFF841 at 79.412 kb on + strand, within GFF841 at 79.412 kb on + strand, within GFF841 at 79.412 kb on + strand, within GFF841 at 79.412 kb on + strand, within GFF841 at 79.412 kb on + strand, within GFF841 at 79.531 kb on + strand at 79.562 kb on + strand at 79.671 kb on + strand at 79.686 kb on + strand at 79.686 kb on + strand at 79.691 kb on + strand at 79.691 kb on + strand at 79.709 kb on + strand, within GFF842 at 79.745 kb on + strand, within GFF842 at 79.846 kb on + strand, within GFF842 at 79.884 kb on + strand, within GFF842 at 79.884 kb on + strand, within GFF842 at 80.065 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 0.5x remove 77,582 + GFF839 0.32 -0.4 77,583 - GFF839 0.32 -0.3 77,583 - GFF839 0.32 -3.0 77,583 - GFF839 0.32 -0.2 77,688 + GFF839 0.53 +0.4 77,707 + GFF839 0.57 -0.2 77,722 - GFF839 0.60 -1.0 77,756 + GFF839 0.66 -0.5 77,756 + GFF839 0.66 -0.2 77,757 - GFF839 0.67 -0.7 77,757 - GFF839 0.67 +0.1 77,757 - GFF839 0.67 -0.9 77,757 - GFF839 0.67 +0.0 77,757 - GFF839 0.67 -1.5 77,757 - GFF839 0.67 -2.2 77,757 - GFF839 0.67 +1.1 77,757 - GFF839 0.67 -0.1 77,757 - GFF839 0.67 -0.3 77,775 + GFF839 0.70 -0.2 77,775 + GFF839 0.70 -0.2 77,776 - GFF839 0.70 -1.2 77,776 - GFF839 0.70 +0.7 77,839 + GFF839 0.83 +0.8 77,859 + GFF839 0.87 +0.9 77,859 + GFF839 0.87 -0.3 77,859 + GFF839 0.87 +0.3 77,859 + GFF839 0.87 -0.9 77,859 + GFF839 0.87 -1.2 77,860 - GFF839 0.87 -1.2 77,860 - GFF839 0.87 -0.9 77,860 - GFF839 0.87 -0.9 77,879 + -1.2 77,880 - +0.1 77,880 - -0.7 77,883 + -0.7 77,883 + -0.9 77,884 - -0.3 77,898 + -0.3 77,898 + -1.7 77,898 + -0.9 77,899 - -0.7 77,899 - -1.2 77,899 - -1.0 77,899 - -0.7 77,902 + -0.3 77,902 + -1.0 77,902 + -1.1 77,930 - +0.1 77,930 - -1.1 77,930 - +0.3 77,980 + GFF840 0.14 -1.0 77,980 + GFF840 0.14 -2.9 77,980 + GFF840 0.14 -1.3 77,980 + GFF840 0.14 -0.3 77,980 + GFF840 0.14 -1.8 77,981 - GFF840 0.15 +0.2 77,981 - GFF840 0.15 -0.9 77,981 - GFF840 0.15 -0.7 77,981 - GFF840 0.15 +0.3 77,981 - GFF840 0.15 -1.4 77,983 + GFF840 0.15 -1.3 77,983 + GFF840 0.15 -1.4 77,983 + GFF840 0.15 +0.1 77,983 + GFF840 0.15 +0.0 77,983 + GFF840 0.15 -0.3 77,983 + GFF840 0.15 -0.1 77,983 + GFF840 0.15 -0.7 77,983 + GFF840 0.15 -0.9 77,983 + GFF840 0.15 +0.6 77,983 + GFF840 0.15 -0.9 77,983 + GFF840 0.15 -0.4 77,983 + GFF840 0.15 -1.7 77,984 - GFF840 0.15 -0.9 77,984 - GFF840 0.15 +0.1 77,984 - GFF840 0.15 -0.9 77,984 - GFF840 0.15 -0.5 77,984 - GFF840 0.15 -1.0 77,984 - GFF840 0.15 -1.4 77,998 + GFF840 0.19 -0.9 77,998 + GFF840 0.19 +1.1 77,998 + GFF840 0.19 -0.3 77,998 + GFF840 0.19 -2.0 77,998 + GFF840 0.19 -1.0 77,998 + GFF840 0.19 +0.8 77,999 - GFF840 0.20 -0.9 77,999 - GFF840 0.20 -1.9 77,999 - GFF840 0.20 +0.1 77,999 - GFF840 0.20 -2.0 77,999 - GFF840 0.20 -0.7 77,999 - GFF840 0.20 -0.9 77,999 - GFF840 0.20 -1.9 78,005 - GFF840 0.21 -1.0 78,029 - GFF840 0.28 -0.1 78,041 + GFF840 0.31 +0.1 78,042 - GFF840 0.31 +0.1 78,042 - GFF840 0.31 -0.3 78,076 + GFF840 0.41 -0.4 78,076 + GFF840 0.41 -0.8 78,095 + GFF840 0.46 -0.6 78,095 + GFF840 0.46 -0.9 78,095 + GFF840 0.46 -0.2 78,095 + GFF840 0.46 -0.2 78,096 - GFF840 0.46 -0.9 78,096 - GFF840 0.46 -1.2 78,097 + GFF840 0.47 -0.7 78,097 + GFF840 0.47 -0.0 78,097 + GFF840 0.47 -0.9 78,097 + GFF840 0.47 -1.0 78,097 + GFF840 0.47 -1.1 78,097 + GFF840 0.47 +0.3 78,098 - GFF840 0.47 +0.1 78,098 - GFF840 0.47 -2.3 78,098 - GFF840 0.47 -0.9 78,098 - GFF840 0.47 -0.8 78,098 - GFF840 0.47 +0.3 78,100 + GFF840 0.47 -1.5 78,100 + GFF840 0.47 -0.5 78,101 - GFF840 0.48 -1.2 78,101 - GFF840 0.48 -0.8 78,101 - GFF840 0.48 +0.1 78,101 - GFF840 0.48 -0.1 78,101 - GFF840 0.48 -1.0 78,101 - GFF840 0.48 -1.2 78,101 - GFF840 0.48 +0.7 78,101 - GFF840 0.48 -0.2 78,101 - GFF840 0.48 -0.6 78,101 - GFF840 0.48 -0.9 78,103 + GFF840 0.48 -0.1 78,148 + GFF840 0.61 -1.7 78,148 + GFF840 0.61 -1.4 78,169 + GFF840 0.66 -0.1 78,172 + GFF840 0.67 -0.2 78,173 - GFF840 0.67 -0.8 78,173 - GFF840 0.67 -1.4 78,173 - GFF840 0.67 +0.3 78,196 - GFF840 0.74 +0.1 78,218 + GFF840 0.80 +1.4 78,218 + GFF840 0.80 -1.2 78,218 + GFF840 0.80 -1.5 78,218 + GFF840 0.80 -0.8 78,219 - GFF840 0.80 -0.3 78,219 - GFF840 0.80 +0.3 78,219 - GFF840 0.80 -0.3 78,279 - +0.7 78,290 + -2.1 78,290 + -0.5 78,290 + -1.4 78,290 + -0.5 78,291 - -1.3 78,291 - +0.3 78,291 - -0.9 78,291 - -1.0 78,332 + +0.1 78,332 + +0.6 78,332 + -0.9 78,332 + +0.0 78,332 + -0.0 78,333 - +0.1 78,333 - -1.0 78,333 - +0.3 78,333 - -0.7 78,333 - -0.4 78,333 - -1.7 78,333 - -0.9 78,335 + -1.6 78,418 + -1.2 78,418 + -0.9 78,419 - -0.4 78,419 - -0.9 78,471 + -1.4 78,483 + -2.0 79,187 + GFF841 0.63 -0.4 79,300 + GFF841 0.74 -0.4 79,321 + GFF841 0.76 -0.2 79,321 + GFF841 0.76 +0.7 79,321 + GFF841 0.76 +0.1 79,321 + GFF841 0.76 +1.5 79,321 + GFF841 0.76 +0.3 79,321 + GFF841 0.76 -1.7 79,321 + GFF841 0.76 +0.5 79,321 + GFF841 0.76 +0.1 79,325 + GFF841 0.77 -0.7 79,408 + GFF841 0.85 +0.7 79,412 + GFF841 0.85 -1.4 79,412 + GFF841 0.85 -0.4 79,412 + GFF841 0.85 -0.2 79,412 + GFF841 0.85 -1.4 79,412 + GFF841 0.85 -1.4 79,412 + GFF841 0.85 -0.6 79,412 + GFF841 0.85 -1.2 79,531 + -1.2 79,562 + -1.4 79,671 + -0.9 79,686 + -0.3 79,686 + -0.3 79,691 + -0.9 79,691 + -0.9 79,709 + GFF842 0.13 -2.4 79,745 + GFF842 0.21 -1.3 79,846 + GFF842 0.43 -0.4 79,884 + GFF842 0.52 -2.7 79,884 + GFF842 0.52 +1.1 80,065 + -1.0
Or see this region's nucleotide sequence