Experiment: Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF66 and GFF67 are separated by 33 nucleotides GFF67 and GFF68 are separated by 20 nucleotides GFF68 and GFF69 are separated by 11 nucleotides GFF69 and GFF70 are separated by 0 nucleotides GFF70 and GFF71 are separated by 140 nucleotides
GFF66 - Beta-barrel assembly-enhancing protease, at 75,790 to 77,343
GFF66
GFF67 - hypothetical protein, at 77,377 to 77,739
GFF67
GFF68 - hypothetical protein, at 77,760 to 78,266
GFF68
GFF69 - Phosphomannomutase/phosphoglucomutase, at 78,278 to 79,684
GFF69
GFF70 - Protein IscX, at 79,685 to 79,879
GFF70
GFF71 - hypothetical protein, at 80,020 to 82,059
GFF71
Position (kb)
78
79
80 Strain fitness (log2 ratio)
-2
-1
0
1 at 77.341 kb on + strand at 77.376 kb on + strand at 77.377 kb on - strand at 77.377 kb on - strand at 77.414 kb on - strand, within GFF67 at 77.468 kb on - strand, within GFF67 at 77.503 kb on + strand, within GFF67 at 77.504 kb on - strand, within GFF67 at 77.506 kb on + strand, within GFF67 at 77.506 kb on + strand, within GFF67 at 77.507 kb on - strand, within GFF67 at 77.507 kb on - strand, within GFF67 at 77.514 kb on + strand, within GFF67 at 77.514 kb on + strand, within GFF67 at 77.737 kb on + strand at 77.737 kb on + strand at 77.737 kb on + strand at 77.737 kb on + strand at 77.752 kb on + strand at 77.752 kb on + strand at 77.762 kb on - strand at 77.794 kb on + strand at 77.794 kb on + strand at 77.794 kb on + strand at 77.795 kb on - strand at 77.796 kb on + strand at 77.796 kb on + strand at 77.797 kb on - strand at 77.797 kb on - strand at 77.913 kb on - strand, within GFF68 at 77.926 kb on + strand, within GFF68 at 77.927 kb on - strand, within GFF68 at 78.083 kb on - strand, within GFF68 at 78.083 kb on - strand, within GFF68 at 78.083 kb on - strand, within GFF68 at 78.157 kb on - strand, within GFF68 at 78.157 kb on - strand, within GFF68 at 78.157 kb on - strand, within GFF68 at 78.157 kb on - strand, within GFF68 at 78.166 kb on - strand, within GFF68 at 78.190 kb on - strand, within GFF68 at 78.190 kb on - strand, within GFF68 at 78.211 kb on + strand, within GFF68 at 78.211 kb on + strand, within GFF68 at 78.212 kb on - strand, within GFF68 at 78.212 kb on - strand, within GFF68 at 78.212 kb on - strand, within GFF68 at 78.212 kb on - strand, within GFF68 at 78.212 kb on - strand, within GFF68 at 78.212 kb on - strand, within GFF68 at 78.314 kb on + strand at 78.315 kb on - strand at 78.432 kb on + strand, within GFF69 at 78.467 kb on + strand, within GFF69 at 78.467 kb on + strand, within GFF69 at 78.468 kb on - strand, within GFF69 at 78.557 kb on - strand, within GFF69 at 78.581 kb on + strand, within GFF69 at 78.581 kb on + strand, within GFF69 at 78.581 kb on + strand, within GFF69 at 78.582 kb on - strand, within GFF69 at 78.639 kb on + strand, within GFF69 at 78.640 kb on - strand, within GFF69 at 78.875 kb on + strand, within GFF69 at 78.875 kb on + strand, within GFF69 at 78.875 kb on + strand, within GFF69 at 78.875 kb on + strand, within GFF69 at 78.875 kb on + strand, within GFF69 at 78.875 kb on + strand, within GFF69 at 78.875 kb on + strand, within GFF69 at 78.875 kb on + strand, within GFF69 at 78.875 kb on + strand, within GFF69 at 78.875 kb on + strand, within GFF69 at 78.875 kb on + strand, within GFF69 at 78.876 kb on - strand, within GFF69 at 78.876 kb on - strand, within GFF69 at 78.876 kb on - strand, within GFF69 at 78.876 kb on - strand, within GFF69 at 78.876 kb on - strand, within GFF69 at 78.876 kb on - strand, within GFF69 at 78.876 kb on - strand, within GFF69 at 78.876 kb on - strand, within GFF69 at 78.876 kb on - strand, within GFF69 at 78.876 kb on - strand, within GFF69 at 78.876 kb on - strand, within GFF69 at 78.877 kb on + strand, within GFF69 at 78.877 kb on + strand, within GFF69 at 78.877 kb on + strand, within GFF69 at 78.878 kb on - strand, within GFF69 at 78.910 kb on + strand, within GFF69 at 78.999 kb on - strand, within GFF69 at 79.104 kb on - strand, within GFF69 at 79.212 kb on + strand, within GFF69 at 79.221 kb on + strand, within GFF69 at 79.221 kb on + strand, within GFF69 at 79.222 kb on - strand, within GFF69 at 79.222 kb on - strand, within GFF69 at 79.222 kb on - strand, within GFF69 at 79.225 kb on + strand, within GFF69 at 79.225 kb on + strand, within GFF69 at 79.226 kb on - strand, within GFF69 at 79.226 kb on - strand, within GFF69 at 79.230 kb on + strand, within GFF69 at 79.231 kb on - strand, within GFF69 at 79.231 kb on - strand, within GFF69 at 79.231 kb on - strand, within GFF69 at 79.231 kb on - strand, within GFF69 at 79.231 kb on - strand, within GFF69 at 79.264 kb on - strand, within GFF69 at 79.342 kb on + strand, within GFF69 at 79.353 kb on + strand, within GFF69 at 79.353 kb on + strand, within GFF69 at 79.354 kb on - strand, within GFF69 at 79.354 kb on - strand, within GFF69 at 79.354 kb on - strand, within GFF69 at 79.354 kb on - strand, within GFF69 at 79.354 kb on - strand, within GFF69 at 79.354 kb on - strand, within GFF69 at 79.367 kb on - strand, within GFF69 at 79.410 kb on + strand, within GFF69 at 79.410 kb on + strand, within GFF69 at 79.410 kb on + strand, within GFF69 at 79.411 kb on - strand, within GFF69 at 79.425 kb on + strand, within GFF69 at 79.425 kb on + strand, within GFF69 at 79.425 kb on + strand, within GFF69 at 79.534 kb on - strand, within GFF69 at 79.549 kb on + strand at 79.549 kb on + strand at 79.549 kb on + strand at 79.549 kb on + strand at 79.550 kb on - strand at 79.550 kb on - strand at 79.550 kb on - strand at 79.648 kb on - strand at 79.648 kb on - strand at 79.687 kb on - strand at 79.700 kb on + strand at 79.839 kb on + strand, within GFF70 at 79.839 kb on + strand, within GFF70 at 79.839 kb on + strand, within GFF70 at 79.839 kb on + strand, within GFF70 at 79.840 kb on - strand, within GFF70 at 79.840 kb on - strand, within GFF70 at 79.840 kb on - strand, within GFF70 at 79.840 kb on - strand, within GFF70 at 79.919 kb on + strand at 79.919 kb on + strand at 79.920 kb on - strand at 79.921 kb on + strand at 79.921 kb on + strand at 79.921 kb on + strand at 79.922 kb on - strand at 79.931 kb on + strand at 79.931 kb on + strand at 79.931 kb on + strand at 79.931 kb on + strand at 79.931 kb on + strand at 79.932 kb on - strand at 79.932 kb on - strand at 79.932 kb on - strand at 79.932 kb on - strand at 79.958 kb on + strand at 80.019 kb on + strand at 80.019 kb on + strand at 80.028 kb on + strand at 80.028 kb on + strand at 80.029 kb on - strand at 80.034 kb on + strand at 80.035 kb on - strand at 80.035 kb on - strand at 80.081 kb on + strand at 80.081 kb on + strand at 80.081 kb on + strand at 80.081 kb on + strand at 80.081 kb on + strand at 80.081 kb on + strand at 80.081 kb on + strand at 80.082 kb on - strand at 80.082 kb on - strand at 80.082 kb on - strand at 80.082 kb on - strand at 80.082 kb on - strand at 80.100 kb on - strand at 80.100 kb on - strand at 80.100 kb on - strand at 80.100 kb on - strand at 80.100 kb on - strand at 80.171 kb on + strand at 80.171 kb on + strand at 80.189 kb on + strand at 80.190 kb on - strand at 80.238 kb on - strand, within GFF71 at 80.238 kb on - strand, within GFF71 at 80.238 kb on - strand, within GFF71 at 80.387 kb on + strand, within GFF71 at 80.387 kb on + strand, within GFF71 at 80.387 kb on + strand, within GFF71 at 80.395 kb on + strand, within GFF71 at 80.395 kb on + strand, within GFF71 at 80.395 kb on + strand, within GFF71 at 80.395 kb on + strand, within GFF71 at 80.395 kb on + strand, within GFF71 at 80.396 kb on - strand, within GFF71 at 80.396 kb on - strand, within GFF71 at 80.396 kb on - strand, within GFF71 at 80.396 kb on - strand, within GFF71 at 80.396 kb on - strand, within GFF71 at 80.396 kb on - strand, within GFF71 at 80.396 kb on - strand, within GFF71 at 80.396 kb on - strand, within GFF71 at 80.396 kb on - strand, within GFF71 at 80.557 kb on - strand, within GFF71 at 80.577 kb on + strand, within GFF71 at 80.660 kb on + strand, within GFF71
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 0.5x remove 77,341 + -1.6 77,376 + +0.1 77,377 - -1.0 77,377 - -1.0 77,414 - GFF67 0.10 +0.5 77,468 - GFF67 0.25 +0.1 77,503 + GFF67 0.35 -1.1 77,504 - GFF67 0.35 -0.4 77,506 + GFF67 0.36 -1.4 77,506 + GFF67 0.36 -2.0 77,507 - GFF67 0.36 -1.1 77,507 - GFF67 0.36 -0.1 77,514 + GFF67 0.38 +0.5 77,514 + GFF67 0.38 -0.9 77,737 + -0.3 77,737 + -0.2 77,737 + -0.8 77,737 + -0.2 77,752 + -0.9 77,752 + -1.0 77,762 - -1.7 77,794 + -0.8 77,794 + -0.7 77,794 + -1.6 77,795 - -1.8 77,796 + -0.6 77,796 + -0.3 77,797 - -0.1 77,797 - +0.2 77,913 - GFF68 0.30 -0.4 77,926 + GFF68 0.33 +0.1 77,927 - GFF68 0.33 -0.6 78,083 - GFF68 0.64 -0.8 78,083 - GFF68 0.64 -0.1 78,083 - GFF68 0.64 -2.0 78,157 - GFF68 0.78 -0.3 78,157 - GFF68 0.78 +0.5 78,157 - GFF68 0.78 -0.5 78,157 - GFF68 0.78 -0.8 78,166 - GFF68 0.80 -0.4 78,190 - GFF68 0.85 -0.1 78,190 - GFF68 0.85 -0.1 78,211 + GFF68 0.89 -0.4 78,211 + GFF68 0.89 -0.1 78,212 - GFF68 0.89 -1.3 78,212 - GFF68 0.89 +0.8 78,212 - GFF68 0.89 -1.4 78,212 - GFF68 0.89 +0.4 78,212 - GFF68 0.89 -1.2 78,212 - GFF68 0.89 +0.2 78,314 + -1.5 78,315 - -0.2 78,432 + GFF69 0.11 +0.0 78,467 + GFF69 0.13 +0.2 78,467 + GFF69 0.13 -0.3 78,468 - GFF69 0.14 -0.3 78,557 - GFF69 0.20 -1.1 78,581 + GFF69 0.22 -0.5 78,581 + GFF69 0.22 -1.6 78,581 + GFF69 0.22 +0.6 78,582 - GFF69 0.22 -0.0 78,639 + GFF69 0.26 -0.8 78,640 - GFF69 0.26 -0.2 78,875 + GFF69 0.42 -0.4 78,875 + GFF69 0.42 +0.2 78,875 + GFF69 0.42 -1.1 78,875 + GFF69 0.42 -0.3 78,875 + GFF69 0.42 -1.6 78,875 + GFF69 0.42 -0.9 78,875 + GFF69 0.42 -0.3 78,875 + GFF69 0.42 +0.2 78,875 + GFF69 0.42 +0.3 78,875 + GFF69 0.42 -0.8 78,875 + GFF69 0.42 +1.0 78,876 - GFF69 0.43 -1.9 78,876 - GFF69 0.43 -1.0 78,876 - GFF69 0.43 -0.7 78,876 - GFF69 0.43 -0.6 78,876 - GFF69 0.43 +0.6 78,876 - GFF69 0.43 -1.1 78,876 - GFF69 0.43 +0.5 78,876 - GFF69 0.43 -1.4 78,876 - GFF69 0.43 -1.4 78,876 - GFF69 0.43 +0.2 78,876 - GFF69 0.43 -0.8 78,877 + GFF69 0.43 +0.5 78,877 + GFF69 0.43 +0.9 78,877 + GFF69 0.43 -0.2 78,878 - GFF69 0.43 -1.0 78,910 + GFF69 0.45 -1.7 78,999 - GFF69 0.51 -0.1 79,104 - GFF69 0.59 -0.8 79,212 + GFF69 0.66 +0.8 79,221 + GFF69 0.67 -0.4 79,221 + GFF69 0.67 -0.6 79,222 - GFF69 0.67 -0.2 79,222 - GFF69 0.67 -0.4 79,222 - GFF69 0.67 +0.2 79,225 + GFF69 0.67 +0.6 79,225 + GFF69 0.67 -0.8 79,226 - GFF69 0.67 -0.9 79,226 - GFF69 0.67 -0.0 79,230 + GFF69 0.68 -1.4 79,231 - GFF69 0.68 +1.5 79,231 - GFF69 0.68 -0.2 79,231 - GFF69 0.68 -0.8 79,231 - GFF69 0.68 -0.5 79,231 - GFF69 0.68 +1.2 79,264 - GFF69 0.70 -1.0 79,342 + GFF69 0.76 +0.0 79,353 + GFF69 0.76 -1.3 79,353 + GFF69 0.76 -0.2 79,354 - GFF69 0.76 -1.1 79,354 - GFF69 0.76 -1.1 79,354 - GFF69 0.76 -0.8 79,354 - GFF69 0.76 +0.2 79,354 - GFF69 0.76 -0.5 79,354 - GFF69 0.76 +0.1 79,367 - GFF69 0.77 -0.0 79,410 + GFF69 0.80 -0.5 79,410 + GFF69 0.80 -1.3 79,410 + GFF69 0.80 +0.8 79,411 - GFF69 0.81 -0.8 79,425 + GFF69 0.82 -1.4 79,425 + GFF69 0.82 -1.0 79,425 + GFF69 0.82 -1.0 79,534 - GFF69 0.89 -0.8 79,549 + -1.1 79,549 + +0.5 79,549 + +0.5 79,549 + -0.8 79,550 - -0.4 79,550 - -2.5 79,550 - +0.8 79,648 - -0.4 79,648 - +0.4 79,687 - +0.1 79,700 + -0.6 79,839 + GFF70 0.79 -0.8 79,839 + GFF70 0.79 -0.6 79,839 + GFF70 0.79 -1.1 79,839 + GFF70 0.79 -0.4 79,840 - GFF70 0.79 +0.6 79,840 - GFF70 0.79 -1.8 79,840 - GFF70 0.79 -1.3 79,840 - GFF70 0.79 -0.9 79,919 + -0.4 79,919 + -0.3 79,920 - -0.6 79,921 + -0.2 79,921 + -1.0 79,921 + +0.2 79,922 - -0.6 79,931 + +0.8 79,931 + -0.8 79,931 + -1.8 79,931 + -0.8 79,931 + -0.6 79,932 - +0.0 79,932 - -0.6 79,932 - -0.8 79,932 - -1.5 79,958 + -2.5 80,019 + -0.8 80,019 + -0.1 80,028 + -0.2 80,028 + -0.1 80,029 - -0.8 80,034 + -0.6 80,035 - +0.2 80,035 - +1.2 80,081 + -1.6 80,081 + -0.4 80,081 + -1.1 80,081 + +0.8 80,081 + -2.7 80,081 + -0.2 80,081 + +0.7 80,082 - -0.1 80,082 - -0.8 80,082 - -0.2 80,082 - -0.5 80,082 - -0.9 80,100 - -0.8 80,100 - -1.1 80,100 - -0.8 80,100 - -0.1 80,100 - +0.0 80,171 + -1.5 80,171 + -0.5 80,189 + +0.2 80,190 - -0.6 80,238 - GFF71 0.11 -0.5 80,238 - GFF71 0.11 -0.4 80,238 - GFF71 0.11 -2.3 80,387 + GFF71 0.18 -0.2 80,387 + GFF71 0.18 +0.8 80,387 + GFF71 0.18 -1.1 80,395 + GFF71 0.18 -1.8 80,395 + GFF71 0.18 -0.2 80,395 + GFF71 0.18 -0.7 80,395 + GFF71 0.18 -0.8 80,395 + GFF71 0.18 -2.5 80,396 - GFF71 0.18 +0.2 80,396 - GFF71 0.18 -1.8 80,396 - GFF71 0.18 -0.1 80,396 - GFF71 0.18 -0.8 80,396 - GFF71 0.18 -1.1 80,396 - GFF71 0.18 -1.1 80,396 - GFF71 0.18 -0.6 80,396 - GFF71 0.18 -1.1 80,396 - GFF71 0.18 -0.2 80,557 - GFF71 0.26 -1.0 80,577 + GFF71 0.27 -0.3 80,660 + GFF71 0.31 +0.1
Or see this region's nucleotide sequence