Experiment: Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2573 and GFF2574 overlap by 4 nucleotides GFF2574 and GFF2575 are separated by 60 nucleotides GFF2575 and GFF2576 are separated by 156 nucleotides GFF2576 and GFF2577 are separated by 112 nucleotides
GFF2573 - Transcription antitermination protein NusB, at 274,226 to 274,696
GFF2573
GFF2574 - 6,7-dimethyl-8-ribityllumazine synthase, at 274,693 to 275,142
GFF2574
GFF2575 - hypothetical protein, at 275,203 to 276,876
GFF2575
GFF2576 - Phosphate regulon transcriptional regulatory protein PhoB, at 277,033 to 277,740
GFF2576
GFF2577 - hypothetical protein, at 277,853 to 279,481
GFF2577
Position (kb) 275
276
277 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 275.198 kb on - strand at 275.204 kb on + strand at 275.204 kb on + strand at 275.205 kb on - strand at 275.249 kb on + strand at 275.249 kb on + strand at 275.288 kb on - strand at 275.293 kb on + strand at 275.294 kb on - strand at 275.294 kb on - strand at 275.294 kb on - strand at 275.396 kb on + strand, within GFF2575 at 275.396 kb on + strand, within GFF2575 at 275.396 kb on + strand, within GFF2575 at 275.396 kb on + strand, within GFF2575 at 275.397 kb on - strand, within GFF2575 at 275.397 kb on - strand, within GFF2575 at 275.397 kb on - strand, within GFF2575 at 275.397 kb on - strand, within GFF2575 at 275.397 kb on - strand, within GFF2575 at 275.397 kb on - strand, within GFF2575 at 275.397 kb on - strand, within GFF2575 at 275.480 kb on + strand, within GFF2575 at 275.480 kb on + strand, within GFF2575 at 275.480 kb on + strand, within GFF2575 at 275.480 kb on + strand, within GFF2575 at 275.481 kb on - strand, within GFF2575 at 275.481 kb on - strand, within GFF2575 at 275.481 kb on - strand, within GFF2575 at 275.481 kb on - strand, within GFF2575 at 275.528 kb on + strand, within GFF2575 at 275.529 kb on - strand, within GFF2575 at 275.529 kb on - strand, within GFF2575 at 275.660 kb on + strand, within GFF2575 at 275.660 kb on + strand, within GFF2575 at 275.661 kb on - strand, within GFF2575 at 275.689 kb on + strand, within GFF2575 at 275.689 kb on + strand, within GFF2575 at 275.701 kb on + strand, within GFF2575 at 275.724 kb on - strand, within GFF2575 at 275.724 kb on - strand, within GFF2575 at 275.738 kb on + strand, within GFF2575 at 275.738 kb on + strand, within GFF2575 at 275.738 kb on + strand, within GFF2575 at 275.738 kb on + strand, within GFF2575 at 275.738 kb on + strand, within GFF2575 at 275.738 kb on + strand, within GFF2575 at 275.738 kb on + strand, within GFF2575 at 275.738 kb on + strand, within GFF2575 at 275.739 kb on - strand, within GFF2575 at 275.739 kb on - strand, within GFF2575 at 275.739 kb on - strand, within GFF2575 at 275.739 kb on - strand, within GFF2575 at 275.739 kb on - strand, within GFF2575 at 275.739 kb on - strand, within GFF2575 at 275.739 kb on - strand, within GFF2575 at 275.739 kb on - strand, within GFF2575 at 275.739 kb on - strand, within GFF2575 at 275.739 kb on - strand, within GFF2575 at 275.739 kb on - strand, within GFF2575 at 275.739 kb on - strand, within GFF2575 at 275.739 kb on - strand, within GFF2575 at 275.739 kb on - strand, within GFF2575 at 275.739 kb on - strand, within GFF2575 at 275.739 kb on - strand, within GFF2575 at 275.739 kb on - strand, within GFF2575 at 275.739 kb on - strand, within GFF2575 at 275.739 kb on - strand, within GFF2575 at 275.739 kb on - strand, within GFF2575 at 275.739 kb on - strand, within GFF2575 at 275.767 kb on - strand, within GFF2575 at 275.771 kb on + strand, within GFF2575 at 275.771 kb on + strand, within GFF2575 at 275.771 kb on + strand, within GFF2575 at 275.771 kb on + strand, within GFF2575 at 275.771 kb on + strand, within GFF2575 at 275.771 kb on + strand, within GFF2575 at 275.772 kb on - strand, within GFF2575 at 275.772 kb on - strand, within GFF2575 at 275.772 kb on - strand, within GFF2575 at 275.772 kb on - strand, within GFF2575 at 275.772 kb on - strand, within GFF2575 at 275.830 kb on - strand, within GFF2575 at 275.935 kb on + strand, within GFF2575 at 275.942 kb on - strand, within GFF2575 at 275.942 kb on - strand, within GFF2575 at 275.963 kb on - strand, within GFF2575 at 275.963 kb on - strand, within GFF2575 at 275.963 kb on - strand, within GFF2575 at 275.985 kb on + strand, within GFF2575 at 275.985 kb on + strand, within GFF2575 at 275.986 kb on - strand, within GFF2575 at 275.986 kb on - strand, within GFF2575 at 275.989 kb on - strand, within GFF2575 at 276.052 kb on - strand, within GFF2575 at 276.070 kb on + strand, within GFF2575 at 276.070 kb on + strand, within GFF2575 at 276.070 kb on + strand, within GFF2575 at 276.070 kb on + strand, within GFF2575 at 276.070 kb on + strand, within GFF2575 at 276.070 kb on + strand, within GFF2575 at 276.071 kb on - strand, within GFF2575 at 276.071 kb on - strand, within GFF2575 at 276.071 kb on - strand, within GFF2575 at 276.071 kb on - strand, within GFF2575 at 276.071 kb on - strand, within GFF2575 at 276.103 kb on + strand, within GFF2575 at 276.104 kb on - strand, within GFF2575 at 276.104 kb on - strand, within GFF2575 at 276.189 kb on - strand, within GFF2575 at 276.280 kb on + strand, within GFF2575 at 276.280 kb on + strand, within GFF2575 at 276.280 kb on + strand, within GFF2575 at 276.281 kb on - strand, within GFF2575 at 276.352 kb on - strand, within GFF2575 at 276.352 kb on - strand, within GFF2575 at 276.352 kb on - strand, within GFF2575 at 276.377 kb on + strand, within GFF2575 at 276.377 kb on + strand, within GFF2575 at 276.377 kb on + strand, within GFF2575 at 276.377 kb on + strand, within GFF2575 at 276.378 kb on - strand, within GFF2575 at 276.382 kb on + strand, within GFF2575 at 276.448 kb on + strand, within GFF2575 at 276.448 kb on + strand, within GFF2575 at 276.448 kb on + strand, within GFF2575 at 276.510 kb on + strand, within GFF2575 at 276.565 kb on + strand, within GFF2575 at 276.565 kb on + strand, within GFF2575 at 276.565 kb on + strand, within GFF2575 at 276.566 kb on - strand, within GFF2575 at 276.582 kb on + strand, within GFF2575 at 276.582 kb on + strand, within GFF2575 at 276.582 kb on + strand, within GFF2575 at 276.584 kb on + strand, within GFF2575 at 276.584 kb on + strand, within GFF2575 at 276.584 kb on + strand, within GFF2575 at 276.584 kb on + strand, within GFF2575 at 276.584 kb on + strand, within GFF2575 at 276.584 kb on + strand, within GFF2575 at 276.584 kb on + strand, within GFF2575 at 276.584 kb on + strand, within GFF2575 at 276.585 kb on - strand, within GFF2575 at 276.585 kb on - strand, within GFF2575 at 276.585 kb on - strand, within GFF2575 at 276.585 kb on - strand, within GFF2575 at 276.585 kb on - strand, within GFF2575 at 276.616 kb on - strand, within GFF2575 at 276.758 kb on + strand at 276.769 kb on + strand at 276.770 kb on - strand at 276.770 kb on - strand at 276.782 kb on - strand at 276.834 kb on + strand at 276.835 kb on - strand at 276.835 kb on - strand at 276.835 kb on - strand at 276.855 kb on + strand at 276.864 kb on + strand at 276.865 kb on - strand at 276.865 kb on - strand at 276.878 kb on + strand at 276.888 kb on - strand at 276.891 kb on + strand at 276.892 kb on - strand at 276.897 kb on + strand at 276.919 kb on + strand at 276.932 kb on + strand at 276.938 kb on + strand at 276.954 kb on + strand at 276.999 kb on + strand at 277.014 kb on - strand at 277.014 kb on - strand at 277.014 kb on - strand at 277.058 kb on - strand at 277.059 kb on - strand at 277.059 kb on - strand at 277.059 kb on - strand at 277.123 kb on - strand, within GFF2576 at 277.160 kb on + strand, within GFF2576 at 277.240 kb on - strand, within GFF2576 at 277.272 kb on + strand, within GFF2576 at 277.273 kb on - strand, within GFF2576 at 277.339 kb on + strand, within GFF2576 at 277.339 kb on + strand, within GFF2576 at 277.339 kb on + strand, within GFF2576 at 277.339 kb on + strand, within GFF2576 at 277.339 kb on + strand, within GFF2576 at 277.340 kb on - strand, within GFF2576 at 277.370 kb on + strand, within GFF2576 at 277.451 kb on + strand, within GFF2576 at 277.482 kb on + strand, within GFF2576 at 277.482 kb on + strand, within GFF2576 at 277.482 kb on + strand, within GFF2576 at 277.482 kb on + strand, within GFF2576 at 277.482 kb on + strand, within GFF2576 at 277.483 kb on - strand, within GFF2576 at 277.483 kb on - strand, within GFF2576 at 277.483 kb on - strand, within GFF2576 at 277.483 kb on - strand, within GFF2576 at 277.483 kb on - strand, within GFF2576 at 277.483 kb on - strand, within GFF2576 at 277.483 kb on - strand, within GFF2576 at 277.629 kb on + strand, within GFF2576 at 277.629 kb on + strand, within GFF2576 at 277.629 kb on + strand, within GFF2576 at 277.630 kb on - strand, within GFF2576 at 277.640 kb on + strand, within GFF2576 at 277.720 kb on - strand at 277.739 kb on - strand at 277.752 kb on + strand at 277.765 kb on + strand at 277.766 kb on - strand at 277.766 kb on - strand at 277.791 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 0.5x remove 275,198 - -0.1 275,204 + +0.5 275,204 + -0.3 275,205 - -1.2 275,249 + -1.3 275,249 + -0.9 275,288 - -0.6 275,293 + -1.2 275,294 - -0.3 275,294 - -1.4 275,294 - -0.3 275,396 + GFF2575 0.12 -0.7 275,396 + GFF2575 0.12 +0.0 275,396 + GFF2575 0.12 -1.8 275,396 + GFF2575 0.12 +0.4 275,397 - GFF2575 0.12 +0.3 275,397 - GFF2575 0.12 -0.1 275,397 - GFF2575 0.12 +0.3 275,397 - GFF2575 0.12 +0.3 275,397 - GFF2575 0.12 -1.7 275,397 - GFF2575 0.12 +0.0 275,397 - GFF2575 0.12 -1.2 275,480 + GFF2575 0.17 -0.7 275,480 + GFF2575 0.17 +0.9 275,480 + GFF2575 0.17 -2.9 275,480 + GFF2575 0.17 -0.5 275,481 - GFF2575 0.17 -0.5 275,481 - GFF2575 0.17 +0.4 275,481 - GFF2575 0.17 -0.7 275,481 - GFF2575 0.17 -1.0 275,528 + GFF2575 0.19 -0.2 275,529 - GFF2575 0.19 -0.3 275,529 - GFF2575 0.19 -0.7 275,660 + GFF2575 0.27 -0.5 275,660 + GFF2575 0.27 -2.2 275,661 - GFF2575 0.27 -2.2 275,689 + GFF2575 0.29 +0.7 275,689 + GFF2575 0.29 +0.7 275,701 + GFF2575 0.30 -1.9 275,724 - GFF2575 0.31 -1.3 275,724 - GFF2575 0.31 +0.7 275,738 + GFF2575 0.32 +0.5 275,738 + GFF2575 0.32 -0.6 275,738 + GFF2575 0.32 -0.1 275,738 + GFF2575 0.32 -0.1 275,738 + GFF2575 0.32 -1.3 275,738 + GFF2575 0.32 -0.5 275,738 + GFF2575 0.32 +0.4 275,738 + GFF2575 0.32 +0.6 275,739 - GFF2575 0.32 -1.2 275,739 - GFF2575 0.32 -0.3 275,739 - GFF2575 0.32 +0.3 275,739 - GFF2575 0.32 -0.1 275,739 - GFF2575 0.32 -0.8 275,739 - GFF2575 0.32 -0.1 275,739 - GFF2575 0.32 -0.3 275,739 - GFF2575 0.32 -0.2 275,739 - GFF2575 0.32 -1.4 275,739 - GFF2575 0.32 -0.1 275,739 - GFF2575 0.32 -0.3 275,739 - GFF2575 0.32 -0.7 275,739 - GFF2575 0.32 -0.3 275,739 - GFF2575 0.32 -1.0 275,739 - GFF2575 0.32 -0.6 275,739 - GFF2575 0.32 -0.7 275,739 - GFF2575 0.32 -1.2 275,739 - GFF2575 0.32 -0.5 275,739 - GFF2575 0.32 -0.5 275,739 - GFF2575 0.32 +1.1 275,739 - GFF2575 0.32 -0.1 275,767 - GFF2575 0.34 -0.5 275,771 + GFF2575 0.34 +0.3 275,771 + GFF2575 0.34 -0.1 275,771 + GFF2575 0.34 -0.3 275,771 + GFF2575 0.34 -1.1 275,771 + GFF2575 0.34 -1.0 275,771 + GFF2575 0.34 +2.1 275,772 - GFF2575 0.34 +0.3 275,772 - GFF2575 0.34 +1.5 275,772 - GFF2575 0.34 -3.3 275,772 - GFF2575 0.34 -0.7 275,772 - GFF2575 0.34 -1.7 275,830 - GFF2575 0.37 +0.3 275,935 + GFF2575 0.44 -0.7 275,942 - GFF2575 0.44 +0.3 275,942 - GFF2575 0.44 -0.7 275,963 - GFF2575 0.45 +0.3 275,963 - GFF2575 0.45 +0.2 275,963 - GFF2575 0.45 +0.3 275,985 + GFF2575 0.47 -1.5 275,985 + GFF2575 0.47 -1.6 275,986 - GFF2575 0.47 -1.1 275,986 - GFF2575 0.47 -1.4 275,989 - GFF2575 0.47 +0.3 276,052 - GFF2575 0.51 -0.7 276,070 + GFF2575 0.52 +0.3 276,070 + GFF2575 0.52 -0.1 276,070 + GFF2575 0.52 -1.7 276,070 + GFF2575 0.52 -0.7 276,070 + GFF2575 0.52 +0.5 276,070 + GFF2575 0.52 +0.1 276,071 - GFF2575 0.52 -0.7 276,071 - GFF2575 0.52 -0.9 276,071 - GFF2575 0.52 -1.1 276,071 - GFF2575 0.52 +0.3 276,071 - GFF2575 0.52 +0.0 276,103 + GFF2575 0.54 -0.1 276,104 - GFF2575 0.54 -1.5 276,104 - GFF2575 0.54 +0.9 276,189 - GFF2575 0.59 +0.3 276,280 + GFF2575 0.64 -1.3 276,280 + GFF2575 0.64 -1.3 276,280 + GFF2575 0.64 +0.3 276,281 - GFF2575 0.64 -1.7 276,352 - GFF2575 0.69 -0.1 276,352 - GFF2575 0.69 +0.5 276,352 - GFF2575 0.69 -1.5 276,377 + GFF2575 0.70 -1.1 276,377 + GFF2575 0.70 -0.5 276,377 + GFF2575 0.70 -1.5 276,377 + GFF2575 0.70 -0.7 276,378 - GFF2575 0.70 -1.3 276,382 + GFF2575 0.70 -0.3 276,448 + GFF2575 0.74 -0.5 276,448 + GFF2575 0.74 -0.3 276,448 + GFF2575 0.74 -0.1 276,510 + GFF2575 0.78 -0.3 276,565 + GFF2575 0.81 -0.6 276,565 + GFF2575 0.81 -0.6 276,565 + GFF2575 0.81 -0.1 276,566 - GFF2575 0.81 -0.4 276,582 + GFF2575 0.82 -2.1 276,582 + GFF2575 0.82 -1.2 276,582 + GFF2575 0.82 -2.3 276,584 + GFF2575 0.82 -2.9 276,584 + GFF2575 0.82 -1.1 276,584 + GFF2575 0.82 -1.3 276,584 + GFF2575 0.82 -1.1 276,584 + GFF2575 0.82 -1.3 276,584 + GFF2575 0.82 -2.1 276,584 + GFF2575 0.82 -0.3 276,584 + GFF2575 0.82 -1.1 276,585 - GFF2575 0.83 -1.2 276,585 - GFF2575 0.83 +0.3 276,585 - GFF2575 0.83 -0.1 276,585 - GFF2575 0.83 -1.3 276,585 - GFF2575 0.83 +1.3 276,616 - GFF2575 0.84 -1.3 276,758 + -0.1 276,769 + -1.3 276,770 - -1.1 276,770 - -0.9 276,782 - -0.7 276,834 + -0.5 276,835 - -0.1 276,835 - -1.5 276,835 - -0.1 276,855 + +0.5 276,864 + -2.1 276,865 - -0.3 276,865 - -0.1 276,878 + -1.3 276,888 - +1.0 276,891 + -0.1 276,892 - -0.4 276,897 + -0.8 276,919 + -1.1 276,932 + -1.1 276,938 + -0.8 276,954 + -0.9 276,999 + -0.0 277,014 - -1.7 277,014 - -0.7 277,014 - +0.0 277,058 - -1.1 277,059 - -1.6 277,059 - -1.1 277,059 - -1.3 277,123 - GFF2576 0.13 -1.5 277,160 + GFF2576 0.18 -0.5 277,240 - GFF2576 0.29 +0.3 277,272 + GFF2576 0.34 -0.7 277,273 - GFF2576 0.34 +0.5 277,339 + GFF2576 0.43 -0.7 277,339 + GFF2576 0.43 -1.1 277,339 + GFF2576 0.43 -0.5 277,339 + GFF2576 0.43 -2.1 277,339 + GFF2576 0.43 -0.4 277,340 - GFF2576 0.43 -1.3 277,370 + GFF2576 0.48 +0.1 277,451 + GFF2576 0.59 -1.4 277,482 + GFF2576 0.63 -0.1 277,482 + GFF2576 0.63 +0.3 277,482 + GFF2576 0.63 -0.7 277,482 + GFF2576 0.63 +0.6 277,482 + GFF2576 0.63 -0.5 277,483 - GFF2576 0.64 -0.3 277,483 - GFF2576 0.64 -0.5 277,483 - GFF2576 0.64 +0.3 277,483 - GFF2576 0.64 -0.6 277,483 - GFF2576 0.64 -1.3 277,483 - GFF2576 0.64 -0.1 277,483 - GFF2576 0.64 +0.3 277,629 + GFF2576 0.84 -1.1 277,629 + GFF2576 0.84 -1.1 277,629 + GFF2576 0.84 +0.3 277,630 - GFF2576 0.84 -1.2 277,640 + GFF2576 0.86 +0.1 277,720 - -2.1 277,739 - -0.1 277,752 + -1.7 277,765 + -0.7 277,766 - -1.4 277,766 - -0.3 277,791 + -1.3
Or see this region's nucleotide sequence