Experiment: Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF236 and GFF237 are separated by 113 nucleotides GFF237 and GFF238 are separated by 259 nucleotides GFF238 and GFF239 are separated by 47 nucleotides GFF239 and GFF240 overlap by 15 nucleotides GFF240 and GFF241 are separated by 98 nucleotides GFF241 and GFF242 are separated by 1 nucleotides
GFF236 - hypothetical protein, at 251,101 to 251,511
GFF236
GFF237 - hypothetical protein, at 251,625 to 251,894
GFF237
GFF238 - hypothetical protein, at 252,154 to 252,273
GFF238
GFF239 - hypothetical protein, at 252,321 to 252,608
GFF239
GFF240 - hypothetical protein, at 252,594 to 252,707
GFF240
GFF241 - hypothetical protein, at 252,806 to 253,048
GFF241
GFF242 - hypothetical protein, at 253,050 to 253,436
GFF242
Position (kb) 252
253 Strain fitness (log2 ratio) -2
-1
0
1 at 251.484 kb on + strand at 251.524 kb on - strand at 251.545 kb on - strand at 251.596 kb on + strand at 251.596 kb on + strand at 251.597 kb on - strand at 251.626 kb on + strand at 251.627 kb on - strand at 251.684 kb on + strand, within GFF237 at 251.684 kb on + strand, within GFF237 at 251.685 kb on - strand, within GFF237 at 251.685 kb on - strand, within GFF237 at 251.685 kb on - strand, within GFF237 at 251.685 kb on - strand, within GFF237 at 251.685 kb on - strand, within GFF237 at 251.685 kb on - strand, within GFF237 at 251.685 kb on - strand, within GFF237 at 251.685 kb on - strand, within GFF237 at 251.688 kb on + strand, within GFF237 at 251.689 kb on - strand, within GFF237 at 251.689 kb on - strand, within GFF237 at 251.689 kb on - strand, within GFF237 at 251.689 kb on - strand, within GFF237 at 251.689 kb on - strand, within GFF237 at 251.731 kb on + strand, within GFF237 at 251.731 kb on + strand, within GFF237 at 251.731 kb on + strand, within GFF237 at 251.732 kb on - strand, within GFF237 at 251.732 kb on - strand, within GFF237 at 251.732 kb on - strand, within GFF237 at 251.732 kb on - strand, within GFF237 at 251.732 kb on - strand, within GFF237 at 251.777 kb on - strand, within GFF237 at 251.817 kb on + strand, within GFF237 at 251.817 kb on + strand, within GFF237 at 251.817 kb on + strand, within GFF237 at 251.817 kb on + strand, within GFF237 at 251.817 kb on + strand, within GFF237 at 251.818 kb on - strand, within GFF237 at 251.818 kb on - strand, within GFF237 at 251.818 kb on - strand, within GFF237 at 251.818 kb on - strand, within GFF237 at 251.818 kb on - strand, within GFF237 at 251.818 kb on - strand, within GFF237 at 251.818 kb on - strand, within GFF237 at 251.825 kb on - strand, within GFF237 at 251.825 kb on - strand, within GFF237 at 251.831 kb on - strand, within GFF237 at 251.831 kb on - strand, within GFF237 at 251.887 kb on + strand at 251.887 kb on + strand at 251.887 kb on + strand at 251.897 kb on + strand at 251.898 kb on - strand at 251.906 kb on + strand at 251.910 kb on + strand at 251.910 kb on + strand at 251.913 kb on - strand at 251.913 kb on - strand at 251.942 kb on + strand at 251.942 kb on + strand at 251.942 kb on + strand at 251.942 kb on + strand at 251.943 kb on - strand at 251.943 kb on - strand at 251.943 kb on - strand at 251.943 kb on - strand at 251.944 kb on + strand at 251.963 kb on + strand at 251.963 kb on + strand at 251.963 kb on + strand at 251.964 kb on - strand at 251.980 kb on + strand at 252.067 kb on - strand at 252.070 kb on + strand at 252.070 kb on + strand at 252.070 kb on + strand at 252.070 kb on + strand at 252.070 kb on + strand at 252.071 kb on - strand at 252.071 kb on - strand at 252.076 kb on + strand at 252.076 kb on + strand at 252.076 kb on + strand at 252.076 kb on + strand at 252.077 kb on - strand at 252.077 kb on - strand at 252.077 kb on - strand at 252.077 kb on - strand at 252.077 kb on - strand at 252.077 kb on - strand at 252.079 kb on - strand at 252.079 kb on - strand at 252.079 kb on - strand at 252.079 kb on - strand at 252.080 kb on + strand at 252.081 kb on - strand at 252.081 kb on - strand at 252.134 kb on - strand at 252.140 kb on + strand at 252.147 kb on - strand at 252.148 kb on + strand at 252.148 kb on + strand at 252.149 kb on - strand at 252.149 kb on - strand at 252.149 kb on - strand at 252.163 kb on - strand at 252.184 kb on - strand, within GFF238 at 252.190 kb on + strand, within GFF238 at 252.190 kb on + strand, within GFF238 at 252.190 kb on + strand, within GFF238 at 252.203 kb on + strand, within GFF238 at 252.203 kb on + strand, within GFF238 at 252.207 kb on + strand, within GFF238 at 252.207 kb on + strand, within GFF238 at 252.207 kb on + strand, within GFF238 at 252.207 kb on + strand, within GFF238 at 252.207 kb on + strand, within GFF238 at 252.208 kb on - strand, within GFF238 at 252.255 kb on - strand, within GFF238 at 252.303 kb on + strand at 252.326 kb on + strand at 252.326 kb on + strand at 252.326 kb on + strand at 252.387 kb on - strand, within GFF239 at 252.418 kb on + strand, within GFF239 at 252.418 kb on + strand, within GFF239 at 252.418 kb on + strand, within GFF239 at 252.418 kb on + strand, within GFF239 at 252.418 kb on + strand, within GFF239 at 252.419 kb on - strand, within GFF239 at 252.419 kb on - strand, within GFF239 at 252.419 kb on - strand, within GFF239 at 252.534 kb on - strand, within GFF239 at 252.595 kb on + strand at 252.621 kb on + strand, within GFF240 at 252.621 kb on + strand, within GFF240 at 252.622 kb on - strand, within GFF240 at 252.622 kb on - strand, within GFF240 at 252.622 kb on - strand, within GFF240 at 252.649 kb on + strand, within GFF240 at 252.666 kb on + strand, within GFF240 at 252.666 kb on + strand, within GFF240 at 252.667 kb on - strand, within GFF240 at 252.678 kb on + strand, within GFF240 at 252.678 kb on + strand, within GFF240 at 252.678 kb on + strand, within GFF240 at 252.679 kb on - strand, within GFF240 at 252.679 kb on - strand, within GFF240 at 252.679 kb on - strand, within GFF240 at 252.679 kb on - strand, within GFF240 at 252.679 kb on - strand, within GFF240 at 252.679 kb on - strand, within GFF240 at 252.679 kb on - strand, within GFF240 at 252.683 kb on + strand, within GFF240 at 252.683 kb on + strand, within GFF240 at 252.683 kb on + strand, within GFF240 at 252.684 kb on - strand, within GFF240 at 252.684 kb on - strand, within GFF240 at 252.684 kb on - strand, within GFF240 at 252.684 kb on - strand, within GFF240 at 252.684 kb on - strand, within GFF240 at 252.684 kb on - strand, within GFF240 at 252.708 kb on - strand at 252.747 kb on + strand at 252.747 kb on + strand at 252.774 kb on - strand at 252.782 kb on + strand at 252.814 kb on + strand at 252.913 kb on - strand, within GFF241 at 252.958 kb on + strand, within GFF241 at 252.958 kb on + strand, within GFF241 at 252.967 kb on + strand, within GFF241 at 252.967 kb on + strand, within GFF241 at 252.967 kb on + strand, within GFF241 at 252.967 kb on + strand, within GFF241 at 253.046 kb on + strand at 253.047 kb on - strand at 253.073 kb on + strand at 253.073 kb on + strand at 253.073 kb on + strand at 253.073 kb on + strand at 253.073 kb on + strand at 253.139 kb on + strand, within GFF242 at 253.140 kb on - strand, within GFF242 at 253.155 kb on + strand, within GFF242 at 253.156 kb on - strand, within GFF242 at 253.217 kb on + strand, within GFF242 at 253.218 kb on - strand, within GFF242 at 253.234 kb on + strand, within GFF242 at 253.283 kb on + strand, within GFF242 at 253.283 kb on + strand, within GFF242 at 253.284 kb on - strand, within GFF242 at 253.325 kb on + strand, within GFF242 at 253.434 kb on + strand at 253.435 kb on - strand at 253.442 kb on + strand at 253.443 kb on - strand at 253.448 kb on + strand at 253.448 kb on + strand at 253.602 kb on + strand at 253.602 kb on + strand at 253.602 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 0.5x remove 251,484 + +0.4 251,524 - -0.1 251,545 - +1.2 251,596 + -0.9 251,596 + +0.2 251,597 - -1.1 251,626 + -0.5 251,627 - -1.0 251,684 + GFF237 0.22 -0.8 251,684 + GFF237 0.22 +1.5 251,685 - GFF237 0.22 +0.3 251,685 - GFF237 0.22 -0.8 251,685 - GFF237 0.22 -0.0 251,685 - GFF237 0.22 -1.2 251,685 - GFF237 0.22 +0.2 251,685 - GFF237 0.22 -1.4 251,685 - GFF237 0.22 +0.0 251,685 - GFF237 0.22 +1.5 251,688 + GFF237 0.23 -1.4 251,689 - GFF237 0.24 -0.6 251,689 - GFF237 0.24 -0.2 251,689 - GFF237 0.24 -0.5 251,689 - GFF237 0.24 -0.5 251,689 - GFF237 0.24 -1.2 251,731 + GFF237 0.39 +0.7 251,731 + GFF237 0.39 -0.5 251,731 + GFF237 0.39 -0.1 251,732 - GFF237 0.40 +1.4 251,732 - GFF237 0.40 -0.4 251,732 - GFF237 0.40 -0.8 251,732 - GFF237 0.40 -2.1 251,732 - GFF237 0.40 -1.7 251,777 - GFF237 0.56 -0.2 251,817 + GFF237 0.71 -0.8 251,817 + GFF237 0.71 -1.1 251,817 + GFF237 0.71 -2.1 251,817 + GFF237 0.71 +0.3 251,817 + GFF237 0.71 -0.3 251,818 - GFF237 0.71 -0.2 251,818 - GFF237 0.71 -2.6 251,818 - GFF237 0.71 +1.5 251,818 - GFF237 0.71 -0.1 251,818 - GFF237 0.71 -0.4 251,818 - GFF237 0.71 -0.8 251,818 - GFF237 0.71 -0.8 251,825 - GFF237 0.74 -0.2 251,825 - GFF237 0.74 -1.3 251,831 - GFF237 0.76 -0.4 251,831 - GFF237 0.76 -0.1 251,887 + -0.5 251,887 + -0.4 251,887 + -0.4 251,897 + -0.8 251,898 - -0.4 251,906 + -0.7 251,910 + +0.6 251,910 + -0.8 251,913 - +1.2 251,913 - -0.3 251,942 + -1.4 251,942 + -1.7 251,942 + -0.8 251,942 + -0.1 251,943 - -0.5 251,943 - -0.6 251,943 - -1.2 251,943 - -0.8 251,944 + -1.0 251,963 + +0.2 251,963 + -1.4 251,963 + +0.4 251,964 - -0.5 251,980 + -1.4 252,067 - -1.1 252,070 + -2.1 252,070 + -0.2 252,070 + -0.4 252,070 + -0.5 252,070 + -0.8 252,071 - -0.2 252,071 - -0.8 252,076 + -0.3 252,076 + -0.1 252,076 + -1.4 252,076 + +1.2 252,077 - -1.4 252,077 - +0.2 252,077 - -0.5 252,077 - -0.4 252,077 - -1.1 252,077 - -0.8 252,079 - -1.1 252,079 - -0.2 252,079 - -1.4 252,079 - -1.2 252,080 + -1.2 252,081 - -1.0 252,081 - -0.9 252,134 - -0.7 252,140 + -0.0 252,147 - +0.2 252,148 + +1.5 252,148 + -1.0 252,149 - -2.0 252,149 - -0.4 252,149 - -0.1 252,163 - -1.4 252,184 - GFF238 0.25 -1.3 252,190 + GFF238 0.30 -0.8 252,190 + GFF238 0.30 -0.6 252,190 + GFF238 0.30 -0.6 252,203 + GFF238 0.41 -2.4 252,203 + GFF238 0.41 +0.0 252,207 + GFF238 0.44 -1.7 252,207 + GFF238 0.44 -0.3 252,207 + GFF238 0.44 -0.4 252,207 + GFF238 0.44 -0.6 252,207 + GFF238 0.44 -1.4 252,208 - GFF238 0.45 -0.5 252,255 - GFF238 0.84 -1.4 252,303 + +0.8 252,326 + +0.8 252,326 + -0.3 252,326 + -0.8 252,387 - GFF239 0.23 -0.6 252,418 + GFF239 0.34 -1.0 252,418 + GFF239 0.34 +0.2 252,418 + GFF239 0.34 -0.8 252,418 + GFF239 0.34 -0.8 252,418 + GFF239 0.34 +0.2 252,419 - GFF239 0.34 -1.7 252,419 - GFF239 0.34 +0.5 252,419 - GFF239 0.34 +0.2 252,534 - GFF239 0.74 -1.6 252,595 + -0.8 252,621 + GFF240 0.24 -1.8 252,621 + GFF240 0.24 -0.3 252,622 - GFF240 0.25 +1.7 252,622 - GFF240 0.25 -0.8 252,622 - GFF240 0.25 -0.2 252,649 + GFF240 0.48 +0.5 252,666 + GFF240 0.63 -0.8 252,666 + GFF240 0.63 -0.4 252,667 - GFF240 0.64 -0.7 252,678 + GFF240 0.74 -0.7 252,678 + GFF240 0.74 -0.0 252,678 + GFF240 0.74 +0.8 252,679 - GFF240 0.75 -0.1 252,679 - GFF240 0.75 -0.8 252,679 - GFF240 0.75 +0.4 252,679 - GFF240 0.75 -0.0 252,679 - GFF240 0.75 -0.3 252,679 - GFF240 0.75 -1.6 252,679 - GFF240 0.75 +0.8 252,683 + GFF240 0.78 -0.2 252,683 + GFF240 0.78 -2.5 252,683 + GFF240 0.78 -0.8 252,684 - GFF240 0.79 -0.6 252,684 - GFF240 0.79 -0.3 252,684 - GFF240 0.79 -0.6 252,684 - GFF240 0.79 -0.1 252,684 - GFF240 0.79 -0.3 252,684 - GFF240 0.79 -0.0 252,708 - -1.4 252,747 + +0.2 252,747 + +0.3 252,774 - -0.8 252,782 + +0.1 252,814 + -0.9 252,913 - GFF241 0.44 -0.9 252,958 + GFF241 0.63 +0.5 252,958 + GFF241 0.63 +0.2 252,967 + GFF241 0.66 -0.8 252,967 + GFF241 0.66 -1.2 252,967 + GFF241 0.66 -1.1 252,967 + GFF241 0.66 -0.6 253,046 + -1.3 253,047 - -2.4 253,073 + -0.3 253,073 + -1.2 253,073 + -0.4 253,073 + +0.6 253,073 + -1.8 253,139 + GFF242 0.23 -0.8 253,140 - GFF242 0.23 -0.0 253,155 + GFF242 0.27 -0.7 253,156 - GFF242 0.27 -0.4 253,217 + GFF242 0.43 -1.3 253,218 - GFF242 0.43 -1.6 253,234 + GFF242 0.48 -0.4 253,283 + GFF242 0.60 -1.0 253,283 + GFF242 0.60 -0.4 253,284 - GFF242 0.60 -1.4 253,325 + GFF242 0.71 -1.2 253,434 + +0.2 253,435 - -1.6 253,442 + -0.8 253,443 - -1.1 253,448 + -1.1 253,448 + -1.4 253,602 + -0.1 253,602 + -0.5 253,602 + +0.0
Or see this region's nucleotide sequence