Experiment: Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1746 and GFF1747 are separated by 84 nucleotides GFF1747 and GFF1748 are separated by 24 nucleotides GFF1748 and GFF1749 are separated by 33 nucleotides
GFF1746 - 2-isopropylmalate synthase, at 384,105 to 385,637
GFF1746
GFF1747 - putative inner membrane transporter YedA, at 385,722 to 386,615
GFF1747
GFF1748 - putative phosphatase, at 386,640 to 387,311
GFF1748
GFF1749 - Cytochrome c biogenesis protein CcsA, at 387,345 to 388,178
GFF1749
Position (kb) 385
386
387 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 384.823 kb on + strand, within GFF1746 at 384.960 kb on - strand, within GFF1746 at 385.073 kb on + strand, within GFF1746 at 385.237 kb on + strand, within GFF1746 at 385.287 kb on + strand, within GFF1746 at 385.287 kb on + strand, within GFF1746 at 385.287 kb on + strand, within GFF1746 at 385.288 kb on - strand, within GFF1746 at 385.449 kb on + strand, within GFF1746 at 385.553 kb on + strand at 385.578 kb on + strand at 385.578 kb on + strand at 385.578 kb on + strand at 385.579 kb on - strand at 385.579 kb on - strand at 385.602 kb on + strand at 385.603 kb on - strand at 385.603 kb on - strand at 385.603 kb on - strand at 385.635 kb on + strand at 385.635 kb on + strand at 385.635 kb on + strand at 385.635 kb on + strand at 385.635 kb on + strand at 385.635 kb on + strand at 385.636 kb on - strand at 385.672 kb on - strand at 385.769 kb on + strand at 385.769 kb on + strand at 385.769 kb on + strand at 385.769 kb on + strand at 385.769 kb on + strand at 385.770 kb on - strand at 385.770 kb on - strand at 385.770 kb on - strand at 385.770 kb on - strand at 385.770 kb on - strand at 385.770 kb on - strand at 385.770 kb on - strand at 385.773 kb on + strand at 385.773 kb on + strand at 385.773 kb on + strand at 385.773 kb on + strand at 385.773 kb on + strand at 385.773 kb on + strand at 385.774 kb on - strand at 385.778 kb on + strand at 385.779 kb on - strand at 385.795 kb on - strand at 385.805 kb on + strand at 385.806 kb on - strand at 385.810 kb on - strand at 385.810 kb on - strand at 385.856 kb on + strand, within GFF1747 at 385.857 kb on - strand, within GFF1747 at 385.876 kb on + strand, within GFF1747 at 385.876 kb on + strand, within GFF1747 at 385.876 kb on + strand, within GFF1747 at 385.876 kb on + strand, within GFF1747 at 385.876 kb on + strand, within GFF1747 at 385.876 kb on + strand, within GFF1747 at 385.876 kb on + strand, within GFF1747 at 385.876 kb on + strand, within GFF1747 at 385.876 kb on + strand, within GFF1747 at 385.876 kb on + strand, within GFF1747 at 385.876 kb on + strand, within GFF1747 at 385.876 kb on + strand, within GFF1747 at 385.876 kb on + strand, within GFF1747 at 385.876 kb on + strand, within GFF1747 at 385.876 kb on + strand, within GFF1747 at 385.876 kb on + strand, within GFF1747 at 385.876 kb on + strand, within GFF1747 at 385.877 kb on - strand, within GFF1747 at 385.877 kb on - strand, within GFF1747 at 385.877 kb on - strand, within GFF1747 at 385.877 kb on - strand, within GFF1747 at 385.877 kb on - strand, within GFF1747 at 385.877 kb on - strand, within GFF1747 at 385.877 kb on - strand, within GFF1747 at 385.878 kb on + strand, within GFF1747 at 385.878 kb on + strand, within GFF1747 at 385.878 kb on + strand, within GFF1747 at 385.878 kb on + strand, within GFF1747 at 385.878 kb on + strand, within GFF1747 at 385.878 kb on + strand, within GFF1747 at 385.878 kb on + strand, within GFF1747 at 385.878 kb on + strand, within GFF1747 at 385.878 kb on + strand, within GFF1747 at 385.878 kb on + strand, within GFF1747 at 385.878 kb on + strand, within GFF1747 at 385.878 kb on + strand, within GFF1747 at 385.878 kb on + strand, within GFF1747 at 385.879 kb on - strand, within GFF1747 at 385.879 kb on - strand, within GFF1747 at 385.879 kb on - strand, within GFF1747 at 385.879 kb on - strand, within GFF1747 at 385.879 kb on - strand, within GFF1747 at 385.879 kb on - strand, within GFF1747 at 385.879 kb on - strand, within GFF1747 at 385.879 kb on - strand, within GFF1747 at 385.879 kb on - strand, within GFF1747 at 386.142 kb on - strand, within GFF1747 at 386.241 kb on - strand, within GFF1747 at 386.241 kb on - strand, within GFF1747 at 386.241 kb on - strand, within GFF1747 at 386.302 kb on + strand, within GFF1747 at 386.302 kb on + strand, within GFF1747 at 386.303 kb on - strand, within GFF1747 at 386.327 kb on + strand, within GFF1747 at 386.327 kb on + strand, within GFF1747 at 386.327 kb on + strand, within GFF1747 at 386.327 kb on + strand, within GFF1747 at 386.327 kb on + strand, within GFF1747 at 386.327 kb on + strand, within GFF1747 at 386.327 kb on + strand, within GFF1747 at 386.327 kb on + strand, within GFF1747 at 386.327 kb on + strand, within GFF1747 at 386.327 kb on + strand, within GFF1747 at 386.328 kb on - strand, within GFF1747 at 386.328 kb on - strand, within GFF1747 at 386.328 kb on - strand, within GFF1747 at 386.328 kb on - strand, within GFF1747 at 386.328 kb on - strand, within GFF1747 at 386.328 kb on - strand, within GFF1747 at 386.328 kb on - strand, within GFF1747 at 386.372 kb on + strand, within GFF1747 at 386.390 kb on + strand, within GFF1747 at 386.390 kb on + strand, within GFF1747 at 386.390 kb on + strand, within GFF1747 at 386.390 kb on + strand, within GFF1747 at 386.390 kb on + strand, within GFF1747 at 386.390 kb on + strand, within GFF1747 at 386.391 kb on - strand, within GFF1747 at 386.391 kb on - strand, within GFF1747 at 386.391 kb on - strand, within GFF1747 at 386.391 kb on - strand, within GFF1747 at 386.391 kb on - strand, within GFF1747 at 386.393 kb on + strand, within GFF1747 at 386.393 kb on + strand, within GFF1747 at 386.409 kb on + strand, within GFF1747 at 386.450 kb on + strand, within GFF1747 at 386.450 kb on + strand, within GFF1747 at 386.515 kb on + strand, within GFF1747 at 386.515 kb on + strand, within GFF1747 at 386.515 kb on + strand, within GFF1747 at 386.515 kb on + strand, within GFF1747 at 386.515 kb on + strand, within GFF1747 at 386.515 kb on + strand, within GFF1747 at 386.515 kb on + strand, within GFF1747 at 386.516 kb on - strand, within GFF1747 at 386.516 kb on - strand, within GFF1747 at 386.516 kb on - strand, within GFF1747 at 386.516 kb on - strand, within GFF1747 at 386.517 kb on + strand, within GFF1747 at 386.517 kb on + strand, within GFF1747 at 386.517 kb on + strand, within GFF1747 at 386.517 kb on + strand, within GFF1747 at 386.517 kb on + strand, within GFF1747 at 386.517 kb on + strand, within GFF1747 at 386.517 kb on + strand, within GFF1747 at 386.517 kb on + strand, within GFF1747 at 386.518 kb on - strand, within GFF1747 at 386.518 kb on - strand, within GFF1747 at 386.518 kb on - strand, within GFF1747 at 386.518 kb on - strand, within GFF1747 at 386.518 kb on - strand, within GFF1747 at 386.518 kb on - strand, within GFF1747 at 386.518 kb on - strand, within GFF1747 at 386.529 kb on + strand at 386.529 kb on + strand at 386.529 kb on + strand at 386.529 kb on + strand at 386.529 kb on + strand at 386.529 kb on + strand at 386.530 kb on - strand at 386.531 kb on + strand at 386.531 kb on + strand at 386.531 kb on + strand at 386.531 kb on + strand at 386.531 kb on + strand at 386.532 kb on - strand at 386.532 kb on - strand at 386.532 kb on - strand at 386.532 kb on - strand at 386.613 kb on + strand at 386.613 kb on + strand at 387.309 kb on + strand at 387.309 kb on + strand at 387.310 kb on - strand at 387.313 kb on + strand at 387.313 kb on - strand at 387.313 kb on - strand at 387.323 kb on + strand at 387.323 kb on + strand at 387.323 kb on + strand at 387.324 kb on - strand at 387.324 kb on - strand at 387.324 kb on - strand at 387.324 kb on - strand at 387.342 kb on + strand at 387.343 kb on - strand at 387.343 kb on - strand at 387.343 kb on - strand at 387.346 kb on + strand at 387.346 kb on + strand at 387.347 kb on - strand at 387.384 kb on + strand at 387.384 kb on + strand at 387.384 kb on + strand at 387.384 kb on + strand at 387.385 kb on - strand at 387.385 kb on - strand at 387.385 kb on - strand at 387.385 kb on - strand at 387.385 kb on - strand at 387.385 kb on - strand at 387.385 kb on - strand at 387.385 kb on - strand at 387.385 kb on - strand at 387.386 kb on + strand at 387.386 kb on + strand at 387.386 kb on + strand at 387.386 kb on + strand at 387.387 kb on - strand at 387.387 kb on - strand at 387.388 kb on + strand at 387.388 kb on + strand at 387.388 kb on + strand at 387.388 kb on + strand at 387.388 kb on + strand at 387.388 kb on + strand at 387.389 kb on - strand at 387.389 kb on - strand at 387.389 kb on - strand at 387.389 kb on - strand at 387.389 kb on - strand at 387.458 kb on + strand, within GFF1749 at 387.459 kb on - strand, within GFF1749 at 387.459 kb on - strand, within GFF1749 at 387.484 kb on + strand, within GFF1749 at 387.484 kb on + strand, within GFF1749 at 387.484 kb on + strand, within GFF1749 at 387.485 kb on - strand, within GFF1749 at 387.561 kb on + strand, within GFF1749 at 387.567 kb on - strand, within GFF1749
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 0.5x remove 384,823 + GFF1746 0.47 -0.8 384,960 - GFF1746 0.56 -1.6 385,073 + GFF1746 0.63 -0.8 385,237 + GFF1746 0.74 -0.6 385,287 + GFF1746 0.77 -0.8 385,287 + GFF1746 0.77 -0.3 385,287 + GFF1746 0.77 -0.5 385,288 - GFF1746 0.77 -0.0 385,449 + GFF1746 0.88 -0.4 385,553 + -0.2 385,578 + -0.1 385,578 + -0.3 385,578 + -1.1 385,579 - -1.0 385,579 - -0.5 385,602 + -0.4 385,603 - -0.4 385,603 - +0.5 385,603 - -0.6 385,635 + -0.8 385,635 + +0.5 385,635 + -0.5 385,635 + -0.6 385,635 + -0.4 385,635 + -0.7 385,636 - -1.6 385,672 - +0.2 385,769 + -0.4 385,769 + -0.1 385,769 + -1.1 385,769 + -1.1 385,769 + +1.0 385,770 - -0.7 385,770 - -0.2 385,770 - -0.5 385,770 - +0.4 385,770 - -0.4 385,770 - -1.1 385,770 - -1.6 385,773 + -1.2 385,773 + -0.5 385,773 + -0.9 385,773 + +0.8 385,773 + +0.9 385,773 + -0.7 385,774 - +0.7 385,778 + -0.8 385,779 - -0.8 385,795 - -0.5 385,805 + -0.0 385,806 - -3.0 385,810 - -1.3 385,810 - -0.4 385,856 + GFF1747 0.15 +0.5 385,857 - GFF1747 0.15 -1.6 385,876 + GFF1747 0.17 -0.4 385,876 + GFF1747 0.17 +0.2 385,876 + GFF1747 0.17 -0.8 385,876 + GFF1747 0.17 -0.2 385,876 + GFF1747 0.17 -0.7 385,876 + GFF1747 0.17 +1.5 385,876 + GFF1747 0.17 +0.0 385,876 + GFF1747 0.17 -0.8 385,876 + GFF1747 0.17 -1.1 385,876 + GFF1747 0.17 -0.5 385,876 + GFF1747 0.17 -0.1 385,876 + GFF1747 0.17 -0.3 385,876 + GFF1747 0.17 -1.4 385,876 + GFF1747 0.17 -0.2 385,876 + GFF1747 0.17 -0.0 385,876 + GFF1747 0.17 -0.1 385,876 + GFF1747 0.17 -1.2 385,877 - GFF1747 0.17 -1.4 385,877 - GFF1747 0.17 -0.4 385,877 - GFF1747 0.17 -1.2 385,877 - GFF1747 0.17 -0.4 385,877 - GFF1747 0.17 +0.2 385,877 - GFF1747 0.17 -0.0 385,877 - GFF1747 0.17 -2.1 385,878 + GFF1747 0.17 -0.8 385,878 + GFF1747 0.17 -0.8 385,878 + GFF1747 0.17 -2.0 385,878 + GFF1747 0.17 -1.5 385,878 + GFF1747 0.17 -0.8 385,878 + GFF1747 0.17 -0.6 385,878 + GFF1747 0.17 -1.0 385,878 + GFF1747 0.17 -0.3 385,878 + GFF1747 0.17 -0.4 385,878 + GFF1747 0.17 -0.5 385,878 + GFF1747 0.17 -1.7 385,878 + GFF1747 0.17 -1.7 385,878 + GFF1747 0.17 -0.3 385,879 - GFF1747 0.18 -0.5 385,879 - GFF1747 0.18 -2.0 385,879 - GFF1747 0.18 +0.4 385,879 - GFF1747 0.18 +0.6 385,879 - GFF1747 0.18 -0.1 385,879 - GFF1747 0.18 +1.0 385,879 - GFF1747 0.18 -0.7 385,879 - GFF1747 0.18 -2.3 385,879 - GFF1747 0.18 +0.3 386,142 - GFF1747 0.47 +0.8 386,241 - GFF1747 0.58 +0.2 386,241 - GFF1747 0.58 +0.2 386,241 - GFF1747 0.58 -2.5 386,302 + GFF1747 0.65 +0.2 386,302 + GFF1747 0.65 -0.2 386,303 - GFF1747 0.65 -1.0 386,327 + GFF1747 0.68 -1.6 386,327 + GFF1747 0.68 -1.0 386,327 + GFF1747 0.68 -0.4 386,327 + GFF1747 0.68 -1.0 386,327 + GFF1747 0.68 -0.2 386,327 + GFF1747 0.68 -0.8 386,327 + GFF1747 0.68 +0.8 386,327 + GFF1747 0.68 -0.4 386,327 + GFF1747 0.68 -0.6 386,327 + GFF1747 0.68 -1.4 386,328 - GFF1747 0.68 -1.3 386,328 - GFF1747 0.68 -2.1 386,328 - GFF1747 0.68 -1.8 386,328 - GFF1747 0.68 +0.2 386,328 - GFF1747 0.68 -1.1 386,328 - GFF1747 0.68 -1.7 386,328 - GFF1747 0.68 -1.5 386,372 + GFF1747 0.73 -0.7 386,390 + GFF1747 0.75 -0.8 386,390 + GFF1747 0.75 +0.5 386,390 + GFF1747 0.75 +0.2 386,390 + GFF1747 0.75 -0.4 386,390 + GFF1747 0.75 -0.2 386,390 + GFF1747 0.75 +0.0 386,391 - GFF1747 0.75 -1.7 386,391 - GFF1747 0.75 -0.6 386,391 - GFF1747 0.75 -1.8 386,391 - GFF1747 0.75 -0.8 386,391 - GFF1747 0.75 -0.8 386,393 + GFF1747 0.75 -0.4 386,393 + GFF1747 0.75 -1.1 386,409 + GFF1747 0.77 -1.1 386,450 + GFF1747 0.81 +1.0 386,450 + GFF1747 0.81 +0.2 386,515 + GFF1747 0.89 +0.3 386,515 + GFF1747 0.89 -1.8 386,515 + GFF1747 0.89 -0.6 386,515 + GFF1747 0.89 -0.2 386,515 + GFF1747 0.89 -0.2 386,515 + GFF1747 0.89 -0.8 386,515 + GFF1747 0.89 -0.3 386,516 - GFF1747 0.89 -0.2 386,516 - GFF1747 0.89 +0.7 386,516 - GFF1747 0.89 +0.5 386,516 - GFF1747 0.89 -1.4 386,517 + GFF1747 0.89 -1.1 386,517 + GFF1747 0.89 +0.4 386,517 + GFF1747 0.89 +0.4 386,517 + GFF1747 0.89 +0.1 386,517 + GFF1747 0.89 -1.3 386,517 + GFF1747 0.89 -0.3 386,517 + GFF1747 0.89 -1.9 386,517 + GFF1747 0.89 -0.8 386,518 - GFF1747 0.89 -1.4 386,518 - GFF1747 0.89 -0.7 386,518 - GFF1747 0.89 -1.6 386,518 - GFF1747 0.89 -0.1 386,518 - GFF1747 0.89 -0.6 386,518 - GFF1747 0.89 -0.8 386,518 - GFF1747 0.89 +0.2 386,529 + -0.5 386,529 + -0.8 386,529 + -0.8 386,529 + -0.6 386,529 + -0.8 386,529 + -0.1 386,530 - +0.0 386,531 + -0.7 386,531 + +0.2 386,531 + +0.2 386,531 + +0.2 386,531 + -1.4 386,532 - +0.3 386,532 - +0.8 386,532 - -1.0 386,532 - -1.0 386,613 + -1.6 386,613 + -0.6 387,309 + -1.1 387,309 + -0.6 387,310 - +0.5 387,313 + +0.7 387,313 - +0.6 387,313 - -2.9 387,323 + -1.1 387,323 + -0.8 387,323 + +0.7 387,324 - -2.4 387,324 - -0.4 387,324 - -0.7 387,324 - -0.0 387,342 + -0.7 387,343 - -0.3 387,343 - -1.1 387,343 - -1.9 387,346 + -1.6 387,346 + +0.2 387,347 - -1.3 387,384 + -1.6 387,384 + -1.6 387,384 + -1.4 387,384 + -0.4 387,385 - -0.5 387,385 - +0.7 387,385 - -0.4 387,385 - -1.1 387,385 - -1.0 387,385 - -0.1 387,385 - -2.7 387,385 - -0.4 387,385 - -0.7 387,386 + -1.3 387,386 + +1.2 387,386 + -1.1 387,386 + -1.7 387,387 - -1.6 387,387 - -1.0 387,388 + -3.4 387,388 + -0.6 387,388 + -0.1 387,388 + -2.0 387,388 + -0.4 387,388 + +0.2 387,389 - +0.5 387,389 - -0.8 387,389 - -1.1 387,389 - -1.0 387,389 - -1.5 387,458 + GFF1749 0.14 -0.4 387,459 - GFF1749 0.14 -1.6 387,459 - GFF1749 0.14 -0.2 387,484 + GFF1749 0.17 -1.1 387,484 + GFF1749 0.17 -1.1 387,484 + GFF1749 0.17 +0.2 387,485 - GFF1749 0.17 -1.4 387,561 + GFF1749 0.26 +0.9 387,567 - GFF1749 0.27 -1.1
Or see this region's nucleotide sequence