Experiment: Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF901 and GFF902 are separated by 122 nucleotides GFF902 and GFF903 are separated by 214 nucleotides
GFF901 - Low affinity potassium transport system protein kup, at 137,459 to 139,342
GFF901
GFF902 - Exodeoxyribonuclease 7 large subunit, at 139,465 to 140,679
GFF902
GFF903 - Membrane-bound lytic murein transglycosylase C, at 140,894 to 141,880
GFF903
Position (kb)
139
140
141 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 138.495 kb on + strand, within GFF901 at 138.496 kb on - strand, within GFF901 at 138.532 kb on + strand, within GFF901 at 138.532 kb on + strand, within GFF901 at 138.552 kb on + strand, within GFF901 at 138.552 kb on + strand, within GFF901 at 138.553 kb on - strand, within GFF901 at 138.633 kb on + strand, within GFF901 at 138.633 kb on + strand, within GFF901 at 138.634 kb on - strand, within GFF901 at 138.723 kb on + strand, within GFF901 at 138.723 kb on + strand, within GFF901 at 138.723 kb on + strand, within GFF901 at 138.723 kb on + strand, within GFF901 at 138.724 kb on - strand, within GFF901 at 138.724 kb on - strand, within GFF901 at 138.789 kb on - strand, within GFF901 at 138.792 kb on - strand, within GFF901 at 138.815 kb on - strand, within GFF901 at 138.815 kb on - strand, within GFF901 at 138.815 kb on - strand, within GFF901 at 138.869 kb on + strand, within GFF901 at 138.869 kb on + strand, within GFF901 at 138.870 kb on - strand, within GFF901 at 138.870 kb on - strand, within GFF901 at 138.870 kb on - strand, within GFF901 at 138.870 kb on - strand, within GFF901 at 138.889 kb on + strand, within GFF901 at 138.889 kb on + strand, within GFF901 at 138.890 kb on - strand, within GFF901 at 138.908 kb on - strand, within GFF901 at 138.987 kb on + strand, within GFF901 at 138.988 kb on - strand, within GFF901 at 138.994 kb on + strand, within GFF901 at 138.995 kb on - strand, within GFF901 at 139.014 kb on - strand, within GFF901 at 139.030 kb on + strand, within GFF901 at 139.057 kb on - strand, within GFF901 at 139.073 kb on + strand, within GFF901 at 139.073 kb on + strand, within GFF901 at 139.073 kb on + strand, within GFF901 at 139.074 kb on - strand, within GFF901 at 139.120 kb on + strand, within GFF901 at 139.131 kb on + strand, within GFF901 at 139.131 kb on + strand, within GFF901 at 139.132 kb on - strand, within GFF901 at 139.132 kb on - strand, within GFF901 at 139.132 kb on - strand, within GFF901 at 139.186 kb on - strand at 139.249 kb on - strand at 139.249 kb on - strand at 139.287 kb on - strand at 139.307 kb on - strand at 139.333 kb on + strand at 139.333 kb on + strand at 139.333 kb on + strand at 139.334 kb on - strand at 139.355 kb on + strand at 139.355 kb on + strand at 139.355 kb on + strand at 139.355 kb on + strand at 139.428 kb on + strand at 139.429 kb on - strand at 139.446 kb on + strand at 139.446 kb on + strand at 139.447 kb on - strand at 139.467 kb on - strand at 139.596 kb on - strand, within GFF902 at 139.649 kb on + strand, within GFF902 at 139.650 kb on - strand, within GFF902 at 139.675 kb on + strand, within GFF902 at 139.675 kb on + strand, within GFF902 at 139.778 kb on + strand, within GFF902 at 139.779 kb on - strand, within GFF902 at 139.779 kb on - strand, within GFF902 at 139.834 kb on + strand, within GFF902 at 139.834 kb on + strand, within GFF902 at 139.834 kb on + strand, within GFF902 at 139.835 kb on - strand, within GFF902 at 139.841 kb on - strand, within GFF902 at 140.022 kb on + strand, within GFF902 at 140.023 kb on - strand, within GFF902 at 140.023 kb on - strand, within GFF902 at 140.023 kb on - strand, within GFF902 at 140.032 kb on + strand, within GFF902 at 140.032 kb on + strand, within GFF902 at 140.033 kb on - strand, within GFF902 at 140.033 kb on - strand, within GFF902 at 140.033 kb on - strand, within GFF902 at 140.033 kb on - strand, within GFF902 at 140.041 kb on + strand, within GFF902 at 140.041 kb on + strand, within GFF902 at 140.041 kb on + strand, within GFF902 at 140.041 kb on + strand, within GFF902 at 140.042 kb on - strand, within GFF902 at 140.042 kb on - strand, within GFF902 at 140.074 kb on - strand, within GFF902 at 140.278 kb on - strand, within GFF902 at 140.309 kb on - strand, within GFF902 at 140.309 kb on - strand, within GFF902 at 140.330 kb on + strand, within GFF902 at 140.330 kb on + strand, within GFF902 at 140.330 kb on + strand, within GFF902 at 140.331 kb on - strand, within GFF902 at 140.331 kb on - strand, within GFF902 at 140.331 kb on - strand, within GFF902 at 140.331 kb on - strand, within GFF902 at 140.331 kb on - strand, within GFF902 at 140.339 kb on + strand, within GFF902 at 140.339 kb on + strand, within GFF902 at 140.386 kb on + strand, within GFF902 at 140.406 kb on - strand, within GFF902 at 140.406 kb on - strand, within GFF902 at 140.406 kb on - strand, within GFF902 at 140.461 kb on + strand, within GFF902 at 140.461 kb on + strand, within GFF902 at 140.462 kb on - strand, within GFF902 at 140.462 kb on - strand, within GFF902 at 140.462 kb on - strand, within GFF902 at 140.464 kb on + strand, within GFF902 at 140.464 kb on + strand, within GFF902 at 140.464 kb on + strand, within GFF902 at 140.464 kb on + strand, within GFF902 at 140.465 kb on - strand, within GFF902 at 140.465 kb on - strand, within GFF902 at 140.465 kb on - strand, within GFF902 at 140.465 kb on - strand, within GFF902 at 140.481 kb on + strand, within GFF902 at 140.481 kb on + strand, within GFF902 at 140.481 kb on + strand, within GFF902 at 140.481 kb on + strand, within GFF902 at 140.666 kb on - strand at 140.673 kb on + strand at 140.679 kb on + strand at 140.679 kb on + strand at 140.680 kb on - strand at 140.680 kb on - strand at 140.680 kb on - strand at 140.680 kb on - strand at 140.680 kb on - strand at 140.680 kb on - strand at 140.717 kb on - strand at 140.785 kb on - strand at 140.785 kb on - strand at 140.787 kb on - strand at 140.787 kb on - strand at 140.787 kb on - strand at 140.887 kb on + strand at 140.887 kb on + strand at 140.887 kb on + strand at 140.887 kb on + strand at 140.887 kb on + strand at 140.887 kb on - strand at 140.888 kb on - strand at 140.929 kb on + strand at 140.930 kb on - strand at 140.942 kb on + strand at 140.942 kb on + strand at 140.943 kb on - strand at 141.027 kb on + strand, within GFF903 at 141.028 kb on - strand, within GFF903 at 141.028 kb on - strand, within GFF903 at 141.028 kb on - strand, within GFF903 at 141.065 kb on + strand, within GFF903 at 141.066 kb on - strand, within GFF903 at 141.066 kb on - strand, within GFF903 at 141.102 kb on - strand, within GFF903 at 141.116 kb on - strand, within GFF903 at 141.170 kb on + strand, within GFF903 at 141.170 kb on + strand, within GFF903 at 141.171 kb on - strand, within GFF903 at 141.171 kb on - strand, within GFF903 at 141.171 kb on - strand, within GFF903 at 141.191 kb on + strand, within GFF903 at 141.191 kb on + strand, within GFF903 at 141.192 kb on - strand, within GFF903 at 141.192 kb on - strand, within GFF903 at 141.251 kb on + strand, within GFF903 at 141.453 kb on - strand, within GFF903 at 141.489 kb on + strand, within GFF903 at 141.490 kb on - strand, within GFF903 at 141.535 kb on + strand, within GFF903 at 141.623 kb on + strand, within GFF903 at 141.623 kb on + strand, within GFF903 at 141.623 kb on + strand, within GFF903 at 141.623 kb on + strand, within GFF903 at 141.623 kb on + strand, within GFF903 at 141.624 kb on - strand, within GFF903 at 141.640 kb on + strand, within GFF903 at 141.641 kb on - strand, within GFF903 at 141.659 kb on + strand, within GFF903 at 141.659 kb on + strand, within GFF903 at 141.660 kb on - strand, within GFF903
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 0.5x remove 138,495 + GFF901 0.55 -1.3 138,496 - GFF901 0.55 -1.6 138,532 + GFF901 0.57 +0.2 138,532 + GFF901 0.57 -0.4 138,552 + GFF901 0.58 -0.0 138,552 + GFF901 0.58 -1.0 138,553 - GFF901 0.58 -2.6 138,633 + GFF901 0.62 -0.3 138,633 + GFF901 0.62 -0.9 138,634 - GFF901 0.62 -0.0 138,723 + GFF901 0.67 -1.7 138,723 + GFF901 0.67 -0.6 138,723 + GFF901 0.67 -1.1 138,723 + GFF901 0.67 -1.3 138,724 - GFF901 0.67 -1.0 138,724 - GFF901 0.67 -1.0 138,789 - GFF901 0.71 -1.5 138,792 - GFF901 0.71 +0.7 138,815 - GFF901 0.72 +0.6 138,815 - GFF901 0.72 +0.7 138,815 - GFF901 0.72 -1.3 138,869 + GFF901 0.75 -0.5 138,869 + GFF901 0.75 -1.0 138,870 - GFF901 0.75 -1.0 138,870 - GFF901 0.75 +1.0 138,870 - GFF901 0.75 -0.4 138,870 - GFF901 0.75 -0.3 138,889 + GFF901 0.76 -2.6 138,889 + GFF901 0.76 +0.2 138,890 - GFF901 0.76 -0.0 138,908 - GFF901 0.77 -0.0 138,987 + GFF901 0.81 -0.0 138,988 - GFF901 0.81 -0.5 138,994 + GFF901 0.81 -1.6 138,995 - GFF901 0.82 -0.0 139,014 - GFF901 0.83 -0.5 139,030 + GFF901 0.83 -0.8 139,057 - GFF901 0.85 -1.3 139,073 + GFF901 0.86 -0.0 139,073 + GFF901 0.86 -0.3 139,073 + GFF901 0.86 -1.0 139,074 - GFF901 0.86 -1.0 139,120 + GFF901 0.88 +0.6 139,131 + GFF901 0.89 -1.6 139,131 + GFF901 0.89 -0.8 139,132 - GFF901 0.89 -1.3 139,132 - GFF901 0.89 -1.6 139,132 - GFF901 0.89 -0.8 139,186 - -1.0 139,249 - -0.0 139,249 - -0.3 139,287 - -1.2 139,307 - -1.0 139,333 + +1.0 139,333 + -0.9 139,333 + +0.7 139,334 - +0.3 139,355 + -1.0 139,355 + -0.0 139,355 + -1.6 139,355 + -2.3 139,428 + -0.3 139,429 - +0.2 139,446 + -0.8 139,446 + -0.6 139,447 - -3.2 139,467 - -1.8 139,596 - GFF902 0.11 -1.3 139,649 + GFF902 0.15 +1.3 139,650 - GFF902 0.15 -1.6 139,675 + GFF902 0.17 -0.1 139,675 + GFF902 0.17 -0.8 139,778 + GFF902 0.26 -0.8 139,779 - GFF902 0.26 -0.0 139,779 - GFF902 0.26 -0.0 139,834 + GFF902 0.30 -0.6 139,834 + GFF902 0.30 -0.6 139,834 + GFF902 0.30 -0.3 139,835 - GFF902 0.30 -0.0 139,841 - GFF902 0.31 -0.4 140,022 + GFF902 0.46 -0.0 140,023 - GFF902 0.46 +0.3 140,023 - GFF902 0.46 -1.0 140,023 - GFF902 0.46 +1.0 140,032 + GFF902 0.47 -1.0 140,032 + GFF902 0.47 -0.0 140,033 - GFF902 0.47 -1.1 140,033 - GFF902 0.47 +0.7 140,033 - GFF902 0.47 -0.4 140,033 - GFF902 0.47 -0.3 140,041 + GFF902 0.47 +0.8 140,041 + GFF902 0.47 -0.5 140,041 + GFF902 0.47 +0.4 140,041 + GFF902 0.47 -0.6 140,042 - GFF902 0.47 +0.5 140,042 - GFF902 0.47 -0.2 140,074 - GFF902 0.50 +0.6 140,278 - GFF902 0.67 -0.9 140,309 - GFF902 0.69 -0.4 140,309 - GFF902 0.69 -1.0 140,330 + GFF902 0.71 -1.0 140,330 + GFF902 0.71 -0.3 140,330 + GFF902 0.71 -0.0 140,331 - GFF902 0.71 -0.5 140,331 - GFF902 0.71 -0.0 140,331 - GFF902 0.71 +0.3 140,331 - GFF902 0.71 -1.5 140,331 - GFF902 0.71 -0.2 140,339 + GFF902 0.72 -2.2 140,339 + GFF902 0.72 -1.0 140,386 + GFF902 0.76 -1.2 140,406 - GFF902 0.77 -0.6 140,406 - GFF902 0.77 -1.0 140,406 - GFF902 0.77 +0.4 140,461 + GFF902 0.82 -1.6 140,461 + GFF902 0.82 -0.2 140,462 - GFF902 0.82 -1.1 140,462 - GFF902 0.82 -0.6 140,462 - GFF902 0.82 +1.0 140,464 + GFF902 0.82 -1.8 140,464 + GFF902 0.82 +0.4 140,464 + GFF902 0.82 +0.7 140,464 + GFF902 0.82 -0.9 140,465 - GFF902 0.82 -0.6 140,465 - GFF902 0.82 -0.5 140,465 - GFF902 0.82 -1.2 140,465 - GFF902 0.82 +0.1 140,481 + GFF902 0.84 +0.7 140,481 + GFF902 0.84 -1.3 140,481 + GFF902 0.84 +0.2 140,481 + GFF902 0.84 -0.3 140,666 - -0.4 140,673 + -0.3 140,679 + -1.6 140,679 + -0.7 140,680 - -2.5 140,680 - -0.6 140,680 - +0.6 140,680 - -0.0 140,680 - -1.0 140,680 - -1.0 140,717 - +0.2 140,785 - -0.4 140,785 - -0.1 140,787 - -1.9 140,787 - -1.0 140,787 - -0.0 140,887 + +1.0 140,887 + -0.4 140,887 + -1.0 140,887 + +1.6 140,887 + -0.0 140,887 - -0.7 140,888 - +0.1 140,929 + -0.6 140,930 - +0.4 140,942 + +0.6 140,942 + +0.2 140,943 - -0.8 141,027 + GFF903 0.13 -1.0 141,028 - GFF903 0.14 -1.0 141,028 - GFF903 0.14 +0.1 141,028 - GFF903 0.14 -2.5 141,065 + GFF903 0.17 -3.2 141,066 - GFF903 0.17 -0.7 141,066 - GFF903 0.17 -2.6 141,102 - GFF903 0.21 +0.4 141,116 - GFF903 0.22 -1.2 141,170 + GFF903 0.28 +0.6 141,170 + GFF903 0.28 -0.3 141,171 - GFF903 0.28 -2.2 141,171 - GFF903 0.28 +0.2 141,171 - GFF903 0.28 +0.4 141,191 + GFF903 0.30 +0.2 141,191 + GFF903 0.30 -0.2 141,192 - GFF903 0.30 -0.6 141,192 - GFF903 0.30 -0.3 141,251 + GFF903 0.36 -0.0 141,453 - GFF903 0.57 -0.9 141,489 + GFF903 0.60 -1.3 141,490 - GFF903 0.60 -1.3 141,535 + GFF903 0.65 -0.2 141,623 + GFF903 0.74 -0.0 141,623 + GFF903 0.74 -0.7 141,623 + GFF903 0.74 -1.3 141,623 + GFF903 0.74 +0.2 141,623 + GFF903 0.74 -0.1 141,624 - GFF903 0.74 +0.7 141,640 + GFF903 0.76 -0.7 141,641 - GFF903 0.76 -0.6 141,659 + GFF903 0.78 -1.6 141,659 + GFF903 0.78 -0.8 141,660 - GFF903 0.78 -0.2
Or see this region's nucleotide sequence