Experiment: Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2078 and GFF2079 are separated by 66 nucleotides GFF2079 and GFF2080 are separated by 40 nucleotides GFF2080 and GFF2081 are separated by 13 nucleotides GFF2081 and GFF2082 are separated by 59 nucleotides
GFF2078 - Cell division protein FtsN, at 124,049 to 124,642
GFF2078
GFF2079 - Thiol:disulfide interchange protein DsbA, at 124,709 to 125,353
GFF2079
GFF2080 - Glutathione-regulated potassium-efflux system protein KefB, at 125,394 to 127,085
GFF2080
GFF2081 - hypothetical protein, at 127,099 to 127,473
GFF2081
GFF2082 - putative oxidoreductase, at 127,533 to 128,357
GFF2082
Position (kb) 125
126
127
128 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 124.600 kb on + strand at 124.600 kb on + strand at 124.640 kb on + strand at 124.691 kb on + strand at 124.693 kb on + strand at 124.693 kb on + strand at 124.693 kb on + strand at 124.693 kb on + strand at 124.693 kb on + strand at 124.693 kb on + strand at 125.345 kb on - strand at 125.381 kb on + strand at 125.381 kb on + strand at 125.381 kb on + strand at 125.381 kb on + strand at 125.381 kb on + strand at 125.382 kb on - strand at 125.382 kb on - strand at 125.382 kb on - strand at 125.452 kb on + strand at 125.453 kb on - strand at 125.482 kb on + strand at 125.482 kb on + strand at 125.483 kb on - strand at 125.483 kb on - strand at 125.565 kb on + strand, within GFF2080 at 125.565 kb on + strand, within GFF2080 at 125.646 kb on + strand, within GFF2080 at 125.646 kb on + strand, within GFF2080 at 125.646 kb on + strand, within GFF2080 at 125.647 kb on - strand, within GFF2080 at 125.647 kb on - strand, within GFF2080 at 125.721 kb on - strand, within GFF2080 at 125.723 kb on + strand, within GFF2080 at 125.724 kb on - strand, within GFF2080 at 125.846 kb on + strand, within GFF2080 at 125.846 kb on + strand, within GFF2080 at 125.846 kb on + strand, within GFF2080 at 125.847 kb on - strand, within GFF2080 at 125.847 kb on - strand, within GFF2080 at 125.847 kb on - strand, within GFF2080 at 125.848 kb on + strand, within GFF2080 at 125.848 kb on + strand, within GFF2080 at 125.848 kb on + strand, within GFF2080 at 125.848 kb on + strand, within GFF2080 at 125.848 kb on + strand, within GFF2080 at 125.848 kb on + strand, within GFF2080 at 125.849 kb on - strand, within GFF2080 at 125.849 kb on - strand, within GFF2080 at 125.849 kb on - strand, within GFF2080 at 125.849 kb on - strand, within GFF2080 at 125.849 kb on - strand, within GFF2080 at 125.849 kb on - strand, within GFF2080 at 125.927 kb on + strand, within GFF2080 at 125.993 kb on + strand, within GFF2080 at 126.002 kb on + strand, within GFF2080 at 126.003 kb on - strand, within GFF2080 at 126.003 kb on - strand, within GFF2080 at 126.003 kb on - strand, within GFF2080 at 126.003 kb on - strand, within GFF2080 at 126.005 kb on + strand, within GFF2080 at 126.006 kb on - strand, within GFF2080 at 126.006 kb on - strand, within GFF2080 at 126.028 kb on - strand, within GFF2080 at 126.028 kb on - strand, within GFF2080 at 126.286 kb on + strand, within GFF2080 at 126.286 kb on + strand, within GFF2080 at 126.286 kb on + strand, within GFF2080 at 126.287 kb on - strand, within GFF2080 at 126.344 kb on + strand, within GFF2080 at 126.344 kb on + strand, within GFF2080 at 126.344 kb on + strand, within GFF2080 at 126.399 kb on + strand, within GFF2080 at 126.399 kb on + strand, within GFF2080 at 126.400 kb on - strand, within GFF2080 at 126.513 kb on - strand, within GFF2080 at 126.559 kb on - strand, within GFF2080 at 126.559 kb on - strand, within GFF2080 at 126.569 kb on + strand, within GFF2080 at 126.580 kb on + strand, within GFF2080 at 126.581 kb on - strand, within GFF2080 at 126.650 kb on + strand, within GFF2080 at 126.809 kb on - strand, within GFF2080 at 126.833 kb on - strand, within GFF2080 at 126.833 kb on - strand, within GFF2080 at 126.833 kb on - strand, within GFF2080 at 126.911 kb on + strand, within GFF2080 at 126.911 kb on + strand, within GFF2080 at 126.912 kb on - strand, within GFF2080 at 126.912 kb on - strand, within GFF2080 at 126.913 kb on + strand, within GFF2080 at 126.913 kb on + strand, within GFF2080 at 126.914 kb on - strand, within GFF2080 at 126.914 kb on - strand, within GFF2080 at 126.923 kb on + strand at 126.923 kb on + strand at 126.925 kb on + strand at 126.925 kb on + strand at 126.926 kb on - strand at 126.926 kb on - strand at 126.926 kb on - strand at 126.952 kb on + strand at 126.953 kb on - strand at 127.012 kb on + strand at 127.012 kb on + strand at 127.012 kb on + strand at 127.013 kb on - strand at 127.013 kb on - strand at 127.013 kb on - strand at 127.013 kb on - strand at 127.013 kb on - strand at 127.092 kb on + strand at 127.093 kb on - strand at 127.093 kb on - strand at 127.093 kb on - strand at 127.125 kb on - strand at 127.125 kb on - strand at 127.236 kb on + strand, within GFF2081 at 127.236 kb on + strand, within GFF2081 at 127.258 kb on + strand, within GFF2081 at 127.259 kb on - strand, within GFF2081 at 127.324 kb on + strand, within GFF2081 at 127.358 kb on + strand, within GFF2081 at 127.358 kb on + strand, within GFF2081 at 127.359 kb on - strand, within GFF2081 at 127.359 kb on - strand, within GFF2081 at 127.359 kb on - strand, within GFF2081 at 127.359 kb on - strand, within GFF2081 at 127.359 kb on - strand, within GFF2081 at 127.359 kb on - strand, within GFF2081 at 127.359 kb on - strand, within GFF2081 at 127.359 kb on - strand, within GFF2081 at 127.359 kb on - strand, within GFF2081 at 127.359 kb on - strand, within GFF2081 at 127.359 kb on - strand, within GFF2081 at 127.359 kb on - strand, within GFF2081 at 127.419 kb on + strand, within GFF2081 at 127.419 kb on + strand, within GFF2081 at 127.433 kb on - strand, within GFF2081 at 127.437 kb on + strand at 127.438 kb on - strand at 127.438 kb on - strand at 127.440 kb on + strand at 127.441 kb on - strand at 127.475 kb on + strand at 127.476 kb on - strand at 127.476 kb on - strand at 127.497 kb on + strand at 127.536 kb on + strand at 127.537 kb on - strand at 127.571 kb on + strand at 127.571 kb on + strand at 127.572 kb on - strand at 127.572 kb on - strand at 127.654 kb on + strand, within GFF2082 at 127.654 kb on + strand, within GFF2082 at 127.654 kb on + strand, within GFF2082 at 127.654 kb on + strand, within GFF2082 at 127.654 kb on + strand, within GFF2082 at 127.654 kb on + strand, within GFF2082 at 127.654 kb on + strand, within GFF2082 at 127.654 kb on + strand, within GFF2082 at 127.655 kb on - strand, within GFF2082 at 127.655 kb on - strand, within GFF2082 at 127.655 kb on - strand, within GFF2082 at 127.655 kb on - strand, within GFF2082 at 127.730 kb on + strand, within GFF2082 at 127.730 kb on + strand, within GFF2082 at 127.730 kb on + strand, within GFF2082 at 127.731 kb on - strand, within GFF2082 at 127.737 kb on + strand, within GFF2082 at 127.737 kb on + strand, within GFF2082 at 127.737 kb on + strand, within GFF2082 at 127.738 kb on - strand, within GFF2082 at 127.750 kb on + strand, within GFF2082 at 127.750 kb on + strand, within GFF2082 at 127.750 kb on + strand, within GFF2082 at 127.750 kb on + strand, within GFF2082 at 127.751 kb on - strand, within GFF2082 at 127.751 kb on - strand, within GFF2082 at 127.751 kb on - strand, within GFF2082 at 127.801 kb on - strand, within GFF2082 at 127.815 kb on - strand, within GFF2082 at 127.937 kb on + strand, within GFF2082 at 127.984 kb on + strand, within GFF2082 at 127.985 kb on - strand, within GFF2082 at 128.048 kb on + strand, within GFF2082 at 128.063 kb on + strand, within GFF2082 at 128.064 kb on - strand, within GFF2082 at 128.067 kb on + strand, within GFF2082 at 128.068 kb on - strand, within GFF2082 at 128.068 kb on - strand, within GFF2082 at 128.068 kb on - strand, within GFF2082
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 0.5x remove 124,600 + -0.2 124,600 + -0.9 124,640 + -0.2 124,691 + +0.7 124,693 + -1.2 124,693 + +0.7 124,693 + +0.3 124,693 + +0.1 124,693 + -1.5 124,693 + -0.7 125,345 - -0.1 125,381 + -0.4 125,381 + -0.2 125,381 + -0.5 125,381 + -0.1 125,381 + +0.5 125,382 - -1.0 125,382 - -0.5 125,382 - -0.7 125,452 + -1.2 125,453 - -1.2 125,482 + +0.7 125,482 + -1.8 125,483 - +0.8 125,483 - -0.1 125,565 + GFF2080 0.10 +1.4 125,565 + GFF2080 0.10 -0.6 125,646 + GFF2080 0.15 -1.5 125,646 + GFF2080 0.15 +0.3 125,646 + GFF2080 0.15 +0.2 125,647 - GFF2080 0.15 -1.6 125,647 - GFF2080 0.15 -1.0 125,721 - GFF2080 0.19 -0.2 125,723 + GFF2080 0.19 +0.1 125,724 - GFF2080 0.20 -0.6 125,846 + GFF2080 0.27 -0.7 125,846 + GFF2080 0.27 -0.1 125,846 + GFF2080 0.27 -2.7 125,847 - GFF2080 0.27 -1.9 125,847 - GFF2080 0.27 -0.4 125,847 - GFF2080 0.27 +0.2 125,848 + GFF2080 0.27 -1.9 125,848 + GFF2080 0.27 -0.9 125,848 + GFF2080 0.27 -1.5 125,848 + GFF2080 0.27 -1.0 125,848 + GFF2080 0.27 -0.4 125,848 + GFF2080 0.27 +0.1 125,849 - GFF2080 0.27 -0.6 125,849 - GFF2080 0.27 -0.7 125,849 - GFF2080 0.27 -0.7 125,849 - GFF2080 0.27 -0.4 125,849 - GFF2080 0.27 -0.9 125,849 - GFF2080 0.27 -1.2 125,927 + GFF2080 0.32 -1.3 125,993 + GFF2080 0.35 -1.6 126,002 + GFF2080 0.36 -1.1 126,003 - GFF2080 0.36 -0.6 126,003 - GFF2080 0.36 -1.5 126,003 - GFF2080 0.36 -1.1 126,003 - GFF2080 0.36 -0.8 126,005 + GFF2080 0.36 -1.4 126,006 - GFF2080 0.36 +0.1 126,006 - GFF2080 0.36 -1.0 126,028 - GFF2080 0.37 -0.7 126,028 - GFF2080 0.37 -0.6 126,286 + GFF2080 0.53 -1.9 126,286 + GFF2080 0.53 -0.9 126,286 + GFF2080 0.53 -1.7 126,287 - GFF2080 0.53 -0.0 126,344 + GFF2080 0.56 -1.2 126,344 + GFF2080 0.56 -0.7 126,344 + GFF2080 0.56 -0.2 126,399 + GFF2080 0.59 -0.5 126,399 + GFF2080 0.59 -1.5 126,400 - GFF2080 0.59 -0.5 126,513 - GFF2080 0.66 -1.3 126,559 - GFF2080 0.69 +0.1 126,559 - GFF2080 0.69 -0.9 126,569 + GFF2080 0.69 +0.1 126,580 + GFF2080 0.70 -0.2 126,581 - GFF2080 0.70 -0.8 126,650 + GFF2080 0.74 -0.5 126,809 - GFF2080 0.84 -0.8 126,833 - GFF2080 0.85 -1.2 126,833 - GFF2080 0.85 -1.1 126,833 - GFF2080 0.85 -1.0 126,911 + GFF2080 0.90 -2.9 126,911 + GFF2080 0.90 -0.2 126,912 - GFF2080 0.90 -0.6 126,912 - GFF2080 0.90 +0.3 126,913 + GFF2080 0.90 +0.1 126,913 + GFF2080 0.90 +0.3 126,914 - GFF2080 0.90 +0.5 126,914 - GFF2080 0.90 -1.3 126,923 + -1.2 126,923 + -1.2 126,925 + -0.5 126,925 + -1.6 126,926 - -0.4 126,926 - +1.1 126,926 - +0.3 126,952 + -0.1 126,953 - +0.2 127,012 + +0.2 127,012 + -1.5 127,012 + -0.2 127,013 - -0.4 127,013 - -0.4 127,013 - -0.3 127,013 - -0.1 127,013 - -2.5 127,092 + -0.5 127,093 - +0.3 127,093 - -0.8 127,093 - +0.4 127,125 - +1.3 127,125 - +0.4 127,236 + GFF2081 0.37 -0.9 127,236 + GFF2081 0.37 -1.7 127,258 + GFF2081 0.42 -1.2 127,259 - GFF2081 0.43 -0.9 127,324 + GFF2081 0.60 -0.9 127,358 + GFF2081 0.69 -0.8 127,358 + GFF2081 0.69 -1.2 127,359 - GFF2081 0.69 -0.9 127,359 - GFF2081 0.69 -1.2 127,359 - GFF2081 0.69 -0.1 127,359 - GFF2081 0.69 +0.3 127,359 - GFF2081 0.69 +0.1 127,359 - GFF2081 0.69 -2.3 127,359 - GFF2081 0.69 +0.0 127,359 - GFF2081 0.69 -0.2 127,359 - GFF2081 0.69 -0.7 127,359 - GFF2081 0.69 -1.1 127,359 - GFF2081 0.69 -0.5 127,359 - GFF2081 0.69 -1.0 127,419 + GFF2081 0.85 +1.1 127,419 + GFF2081 0.85 +1.1 127,433 - GFF2081 0.89 -1.6 127,437 + -1.2 127,438 - -0.4 127,438 - -0.9 127,440 + -0.4 127,441 - -0.4 127,475 + -0.5 127,476 - -0.7 127,476 - -1.2 127,497 + +0.7 127,536 + -0.8 127,537 - -0.2 127,571 + -0.9 127,571 + +0.1 127,572 - +0.3 127,572 - -0.9 127,654 + GFF2082 0.15 -0.4 127,654 + GFF2082 0.15 -1.8 127,654 + GFF2082 0.15 -1.2 127,654 + GFF2082 0.15 +1.1 127,654 + GFF2082 0.15 -0.5 127,654 + GFF2082 0.15 +0.1 127,654 + GFF2082 0.15 -1.3 127,654 + GFF2082 0.15 +0.3 127,655 - GFF2082 0.15 -0.6 127,655 - GFF2082 0.15 -1.7 127,655 - GFF2082 0.15 -0.7 127,655 - GFF2082 0.15 -0.3 127,730 + GFF2082 0.24 +0.2 127,730 + GFF2082 0.24 -0.5 127,730 + GFF2082 0.24 -0.5 127,731 - GFF2082 0.24 -1.4 127,737 + GFF2082 0.25 -1.2 127,737 + GFF2082 0.25 -0.5 127,737 + GFF2082 0.25 -0.9 127,738 - GFF2082 0.25 -1.2 127,750 + GFF2082 0.26 -1.2 127,750 + GFF2082 0.26 -1.6 127,750 + GFF2082 0.26 -1.2 127,750 + GFF2082 0.26 +0.1 127,751 - GFF2082 0.26 -1.7 127,751 - GFF2082 0.26 -0.2 127,751 - GFF2082 0.26 +0.7 127,801 - GFF2082 0.32 +0.3 127,815 - GFF2082 0.34 -2.4 127,937 + GFF2082 0.49 -0.7 127,984 + GFF2082 0.55 -0.8 127,985 - GFF2082 0.55 +0.1 128,048 + GFF2082 0.62 -0.9 128,063 + GFF2082 0.64 +0.1 128,064 - GFF2082 0.64 -0.9 128,067 + GFF2082 0.65 -0.7 128,068 - GFF2082 0.65 -1.0 128,068 - GFF2082 0.65 -0.9 128,068 - GFF2082 0.65 -1.1
Or see this region's nucleotide sequence