Experiment: Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1515 and GFF1516 are separated by 12 nucleotides GFF1516 and GFF1517 overlap by 4 nucleotides GFF1517 and GFF1518 are separated by 277 nucleotides
GFF1515 - DNA polymerase III subunit chi, at 157,173 to 157,601
GFF1515
GFF1516 - hypothetical protein, at 157,614 to 158,567
GFF1516
GFF1517 - Exodeoxyribonuclease III, at 158,564 to 159,340
GFF1517
GFF1518 - hypothetical protein, at 159,618 to 163,307
GFF1518
Position (kb) 158
159
160 Strain fitness (log2 ratio) -2
-1
0
1 at 157.574 kb on + strand at 157.574 kb on + strand at 157.574 kb on + strand at 157.574 kb on + strand at 157.575 kb on - strand at 157.729 kb on + strand, within GFF1516 at 157.819 kb on - strand, within GFF1516 at 157.879 kb on + strand, within GFF1516 at 157.879 kb on + strand, within GFF1516 at 157.879 kb on + strand, within GFF1516 at 157.879 kb on + strand, within GFF1516 at 157.879 kb on + strand, within GFF1516 at 157.879 kb on + strand, within GFF1516 at 157.879 kb on + strand, within GFF1516 at 157.879 kb on + strand, within GFF1516 at 157.879 kb on + strand, within GFF1516 at 157.879 kb on + strand, within GFF1516 at 157.879 kb on + strand, within GFF1516 at 157.879 kb on + strand, within GFF1516 at 157.879 kb on + strand, within GFF1516 at 157.880 kb on - strand, within GFF1516 at 157.880 kb on - strand, within GFF1516 at 157.880 kb on - strand, within GFF1516 at 157.880 kb on - strand, within GFF1516 at 157.880 kb on - strand, within GFF1516 at 158.063 kb on + strand, within GFF1516 at 158.063 kb on + strand, within GFF1516 at 158.063 kb on + strand, within GFF1516 at 158.145 kb on - strand, within GFF1516 at 158.215 kb on + strand, within GFF1516 at 158.215 kb on + strand, within GFF1516 at 158.215 kb on + strand, within GFF1516 at 158.215 kb on + strand, within GFF1516 at 158.216 kb on - strand, within GFF1516 at 158.216 kb on - strand, within GFF1516 at 158.269 kb on + strand, within GFF1516 at 158.269 kb on + strand, within GFF1516 at 158.269 kb on + strand, within GFF1516 at 158.270 kb on - strand, within GFF1516 at 158.337 kb on + strand, within GFF1516 at 158.338 kb on - strand, within GFF1516 at 158.338 kb on - strand, within GFF1516 at 158.339 kb on + strand, within GFF1516 at 158.339 kb on + strand, within GFF1516 at 158.339 kb on + strand, within GFF1516 at 158.340 kb on - strand, within GFF1516 at 158.340 kb on - strand, within GFF1516 at 158.340 kb on - strand, within GFF1516 at 158.364 kb on - strand, within GFF1516 at 158.510 kb on + strand at 158.510 kb on + strand at 158.510 kb on + strand at 158.511 kb on - strand at 158.511 kb on - strand at 158.511 kb on - strand at 158.511 kb on - strand at 158.566 kb on - strand at 158.602 kb on + strand at 158.602 kb on + strand at 158.602 kb on + strand at 158.602 kb on + strand at 158.603 kb on - strand at 158.603 kb on - strand at 158.639 kb on - strand at 158.639 kb on - strand at 158.642 kb on + strand, within GFF1517 at 158.642 kb on + strand, within GFF1517 at 158.642 kb on + strand, within GFF1517 at 158.643 kb on - strand, within GFF1517 at 158.643 kb on - strand, within GFF1517 at 158.643 kb on - strand, within GFF1517 at 158.643 kb on - strand, within GFF1517 at 158.710 kb on + strand, within GFF1517 at 158.710 kb on + strand, within GFF1517 at 158.711 kb on - strand, within GFF1517 at 158.711 kb on - strand, within GFF1517 at 158.737 kb on - strand, within GFF1517 at 158.749 kb on + strand, within GFF1517 at 158.770 kb on + strand, within GFF1517 at 158.785 kb on + strand, within GFF1517 at 158.786 kb on - strand, within GFF1517 at 158.786 kb on - strand, within GFF1517 at 158.786 kb on - strand, within GFF1517 at 158.786 kb on - strand, within GFF1517 at 158.798 kb on + strand, within GFF1517 at 158.840 kb on + strand, within GFF1517 at 158.841 kb on - strand, within GFF1517 at 158.841 kb on - strand, within GFF1517 at 158.899 kb on + strand, within GFF1517 at 158.899 kb on + strand, within GFF1517 at 158.904 kb on + strand, within GFF1517 at 158.904 kb on + strand, within GFF1517 at 158.904 kb on + strand, within GFF1517 at 158.905 kb on - strand, within GFF1517 at 158.905 kb on - strand, within GFF1517 at 158.971 kb on + strand, within GFF1517 at 158.971 kb on + strand, within GFF1517 at 158.971 kb on + strand, within GFF1517 at 158.972 kb on - strand, within GFF1517 at 158.972 kb on - strand, within GFF1517 at 158.988 kb on + strand, within GFF1517 at 158.988 kb on + strand, within GFF1517 at 158.988 kb on + strand, within GFF1517 at 158.988 kb on + strand, within GFF1517 at 158.988 kb on + strand, within GFF1517 at 158.988 kb on + strand, within GFF1517 at 158.989 kb on - strand, within GFF1517 at 158.989 kb on - strand, within GFF1517 at 158.994 kb on + strand, within GFF1517 at 158.994 kb on + strand, within GFF1517 at 158.994 kb on + strand, within GFF1517 at 158.994 kb on + strand, within GFF1517 at 158.995 kb on - strand, within GFF1517 at 158.995 kb on - strand, within GFF1517 at 158.995 kb on - strand, within GFF1517 at 158.995 kb on - strand, within GFF1517 at 158.995 kb on - strand, within GFF1517 at 158.995 kb on - strand, within GFF1517 at 159.008 kb on + strand, within GFF1517 at 159.008 kb on + strand, within GFF1517 at 159.009 kb on - strand, within GFF1517 at 159.031 kb on - strand, within GFF1517 at 159.056 kb on + strand, within GFF1517 at 159.056 kb on + strand, within GFF1517 at 159.056 kb on + strand, within GFF1517 at 159.056 kb on + strand, within GFF1517 at 159.056 kb on + strand, within GFF1517 at 159.056 kb on + strand, within GFF1517 at 159.056 kb on + strand, within GFF1517 at 159.056 kb on + strand, within GFF1517 at 159.056 kb on + strand, within GFF1517 at 159.056 kb on + strand, within GFF1517 at 159.056 kb on + strand, within GFF1517 at 159.057 kb on - strand, within GFF1517 at 159.057 kb on - strand, within GFF1517 at 159.057 kb on - strand, within GFF1517 at 159.057 kb on - strand, within GFF1517 at 159.057 kb on - strand, within GFF1517 at 159.057 kb on - strand, within GFF1517 at 159.057 kb on - strand, within GFF1517 at 159.057 kb on - strand, within GFF1517 at 159.057 kb on - strand, within GFF1517 at 159.057 kb on - strand, within GFF1517 at 159.057 kb on - strand, within GFF1517 at 159.071 kb on + strand, within GFF1517 at 159.072 kb on - strand, within GFF1517 at 159.093 kb on + strand, within GFF1517 at 159.093 kb on + strand, within GFF1517 at 159.094 kb on - strand, within GFF1517 at 159.094 kb on - strand, within GFF1517 at 159.094 kb on - strand, within GFF1517 at 159.094 kb on - strand, within GFF1517 at 159.122 kb on + strand, within GFF1517 at 159.122 kb on + strand, within GFF1517 at 159.123 kb on - strand, within GFF1517 at 159.154 kb on + strand, within GFF1517 at 159.154 kb on + strand, within GFF1517 at 159.154 kb on + strand, within GFF1517 at 159.154 kb on + strand, within GFF1517 at 159.157 kb on - strand, within GFF1517 at 159.157 kb on - strand, within GFF1517 at 159.157 kb on - strand, within GFF1517 at 159.157 kb on - strand, within GFF1517 at 159.157 kb on - strand, within GFF1517 at 159.157 kb on - strand, within GFF1517 at 159.186 kb on + strand, within GFF1517 at 159.187 kb on - strand, within GFF1517 at 159.211 kb on - strand, within GFF1517 at 159.211 kb on - strand, within GFF1517 at 159.223 kb on + strand, within GFF1517 at 159.254 kb on + strand, within GFF1517 at 159.254 kb on + strand, within GFF1517 at 159.254 kb on + strand, within GFF1517 at 159.254 kb on + strand, within GFF1517 at 159.254 kb on + strand, within GFF1517 at 159.254 kb on + strand, within GFF1517 at 159.254 kb on + strand, within GFF1517 at 159.254 kb on + strand, within GFF1517 at 159.254 kb on + strand, within GFF1517 at 159.254 kb on + strand, within GFF1517 at 159.254 kb on + strand, within GFF1517 at 159.255 kb on - strand, within GFF1517 at 159.255 kb on - strand, within GFF1517 at 159.255 kb on - strand, within GFF1517 at 159.255 kb on - strand, within GFF1517 at 159.255 kb on - strand, within GFF1517 at 159.255 kb on - strand, within GFF1517 at 159.316 kb on + strand at 159.316 kb on + strand at 159.316 kb on + strand at 159.319 kb on + strand at 159.319 kb on + strand at 159.319 kb on + strand at 159.320 kb on - strand at 159.320 kb on - strand at 159.328 kb on + strand at 159.328 kb on + strand at 159.400 kb on - strand at 159.455 kb on + strand at 159.455 kb on + strand at 159.475 kb on - strand at 159.515 kb on + strand at 159.537 kb on - strand at 159.537 kb on - strand at 159.537 kb on - strand at 159.547 kb on + strand at 159.547 kb on + strand at 159.548 kb on - strand at 159.549 kb on + strand at 159.549 kb on + strand at 159.550 kb on - strand at 159.553 kb on + strand at 159.553 kb on + strand at 159.553 kb on + strand at 159.553 kb on + strand at 159.553 kb on + strand at 159.553 kb on + strand at 159.553 kb on + strand at 159.554 kb on - strand at 159.554 kb on - strand at 159.554 kb on - strand at 159.594 kb on - strand at 159.715 kb on + strand at 159.716 kb on - strand at 159.754 kb on - strand at 159.756 kb on + strand at 159.757 kb on - strand at 159.757 kb on - strand at 159.777 kb on + strand at 159.777 kb on + strand at 159.777 kb on + strand at 159.777 kb on + strand at 159.868 kb on - strand at 159.954 kb on + strand at 159.954 kb on + strand at 159.954 kb on + strand at 159.954 kb on + strand at 160.019 kb on + strand, within GFF1518 at 160.019 kb on + strand, within GFF1518 at 160.019 kb on + strand, within GFF1518 at 160.019 kb on + strand, within GFF1518 at 160.019 kb on + strand, within GFF1518 at 160.019 kb on + strand, within GFF1518 at 160.020 kb on - strand, within GFF1518 at 160.020 kb on - strand, within GFF1518 at 160.063 kb on + strand, within GFF1518 at 160.063 kb on + strand, within GFF1518 at 160.063 kb on + strand, within GFF1518 at 160.161 kb on + strand, within GFF1518 at 160.162 kb on - strand, within GFF1518 at 160.186 kb on + strand, within GFF1518 at 160.186 kb on + strand, within GFF1518 at 160.186 kb on + strand, within GFF1518 at 160.187 kb on - strand, within GFF1518 at 160.201 kb on - strand, within GFF1518 at 160.201 kb on - strand, within GFF1518 at 160.245 kb on + strand, within GFF1518 at 160.255 kb on + strand, within GFF1518 at 160.255 kb on + strand, within GFF1518 at 160.255 kb on + strand, within GFF1518 at 160.255 kb on + strand, within GFF1518 at 160.256 kb on - strand, within GFF1518 at 160.339 kb on - strand, within GFF1518 at 160.339 kb on - strand, within GFF1518
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 0.5x remove 157,574 + +0.3 157,574 + -0.6 157,574 + -0.7 157,574 + -0.2 157,575 - -1.0 157,729 + GFF1516 0.12 -0.3 157,819 - GFF1516 0.21 -0.6 157,879 + GFF1516 0.28 -0.9 157,879 + GFF1516 0.28 -0.4 157,879 + GFF1516 0.28 -1.3 157,879 + GFF1516 0.28 -2.5 157,879 + GFF1516 0.28 +0.3 157,879 + GFF1516 0.28 -1.0 157,879 + GFF1516 0.28 -0.6 157,879 + GFF1516 0.28 -1.0 157,879 + GFF1516 0.28 -1.0 157,879 + GFF1516 0.28 -1.8 157,879 + GFF1516 0.28 -0.0 157,879 + GFF1516 0.28 +0.8 157,879 + GFF1516 0.28 -1.4 157,880 - GFF1516 0.28 -1.2 157,880 - GFF1516 0.28 +1.0 157,880 - GFF1516 0.28 +0.3 157,880 - GFF1516 0.28 -0.0 157,880 - GFF1516 0.28 -2.2 158,063 + GFF1516 0.47 -0.4 158,063 + GFF1516 0.47 -2.7 158,063 + GFF1516 0.47 +0.6 158,145 - GFF1516 0.56 -0.3 158,215 + GFF1516 0.63 -0.6 158,215 + GFF1516 0.63 -0.2 158,215 + GFF1516 0.63 -1.2 158,215 + GFF1516 0.63 +0.1 158,216 - GFF1516 0.63 -0.7 158,216 - GFF1516 0.63 -0.6 158,269 + GFF1516 0.69 -1.2 158,269 + GFF1516 0.69 +0.5 158,269 + GFF1516 0.69 -1.5 158,270 - GFF1516 0.69 -0.0 158,337 + GFF1516 0.76 -0.5 158,338 - GFF1516 0.76 -0.7 158,338 - GFF1516 0.76 -0.4 158,339 + GFF1516 0.76 -1.6 158,339 + GFF1516 0.76 -2.2 158,339 + GFF1516 0.76 -0.7 158,340 - GFF1516 0.76 -0.5 158,340 - GFF1516 0.76 -1.8 158,340 - GFF1516 0.76 -2.5 158,364 - GFF1516 0.79 -2.1 158,510 + -0.3 158,510 + -0.4 158,510 + -1.3 158,511 - -0.0 158,511 - -0.1 158,511 - -0.7 158,511 - -0.8 158,566 - +0.6 158,602 + -1.5 158,602 + +0.4 158,602 + -0.2 158,602 + +0.7 158,603 - -0.6 158,603 - -2.3 158,639 - +0.1 158,639 - -1.0 158,642 + GFF1517 0.10 -0.9 158,642 + GFF1517 0.10 -0.4 158,642 + GFF1517 0.10 -1.0 158,643 - GFF1517 0.10 +0.3 158,643 - GFF1517 0.10 -1.2 158,643 - GFF1517 0.10 -1.8 158,643 - GFF1517 0.10 -0.6 158,710 + GFF1517 0.19 -1.0 158,710 + GFF1517 0.19 -1.0 158,711 - GFF1517 0.19 -1.3 158,711 - GFF1517 0.19 -0.3 158,737 - GFF1517 0.22 -1.0 158,749 + GFF1517 0.24 -0.8 158,770 + GFF1517 0.27 -1.0 158,785 + GFF1517 0.28 -0.8 158,786 - GFF1517 0.29 -1.2 158,786 - GFF1517 0.29 +0.2 158,786 - GFF1517 0.29 -1.2 158,786 - GFF1517 0.29 -0.8 158,798 + GFF1517 0.30 -1.5 158,840 + GFF1517 0.36 -1.9 158,841 - GFF1517 0.36 -0.0 158,841 - GFF1517 0.36 -1.5 158,899 + GFF1517 0.43 -1.0 158,899 + GFF1517 0.43 -0.5 158,904 + GFF1517 0.44 -1.6 158,904 + GFF1517 0.44 +0.1 158,904 + GFF1517 0.44 -0.0 158,905 - GFF1517 0.44 -1.3 158,905 - GFF1517 0.44 -1.6 158,971 + GFF1517 0.52 -2.4 158,971 + GFF1517 0.52 -0.1 158,971 + GFF1517 0.52 -1.0 158,972 - GFF1517 0.53 -0.0 158,972 - GFF1517 0.53 -1.0 158,988 + GFF1517 0.55 -0.6 158,988 + GFF1517 0.55 -0.7 158,988 + GFF1517 0.55 -0.6 158,988 + GFF1517 0.55 -0.5 158,988 + GFF1517 0.55 -1.0 158,988 + GFF1517 0.55 -0.6 158,989 - GFF1517 0.55 -1.1 158,989 - GFF1517 0.55 -2.3 158,994 + GFF1517 0.55 -2.0 158,994 + GFF1517 0.55 -0.5 158,994 + GFF1517 0.55 -1.7 158,994 + GFF1517 0.55 -0.0 158,995 - GFF1517 0.55 -1.6 158,995 - GFF1517 0.55 +0.5 158,995 - GFF1517 0.55 -1.8 158,995 - GFF1517 0.55 -0.2 158,995 - GFF1517 0.55 +1.3 158,995 - GFF1517 0.55 -0.5 159,008 + GFF1517 0.57 +0.2 159,008 + GFF1517 0.57 -0.3 159,009 - GFF1517 0.57 -2.2 159,031 - GFF1517 0.60 -0.5 159,056 + GFF1517 0.63 -0.6 159,056 + GFF1517 0.63 -0.6 159,056 + GFF1517 0.63 -1.0 159,056 + GFF1517 0.63 -0.8 159,056 + GFF1517 0.63 -1.8 159,056 + GFF1517 0.63 -0.0 159,056 + GFF1517 0.63 -1.6 159,056 + GFF1517 0.63 -2.2 159,056 + GFF1517 0.63 -0.7 159,056 + GFF1517 0.63 -0.0 159,056 + GFF1517 0.63 -2.0 159,057 - GFF1517 0.63 -0.4 159,057 - GFF1517 0.63 -0.0 159,057 - GFF1517 0.63 -0.6 159,057 - GFF1517 0.63 -1.0 159,057 - GFF1517 0.63 -0.9 159,057 - GFF1517 0.63 -1.0 159,057 - GFF1517 0.63 -0.6 159,057 - GFF1517 0.63 -0.0 159,057 - GFF1517 0.63 -0.4 159,057 - GFF1517 0.63 -0.0 159,057 - GFF1517 0.63 -0.6 159,071 + GFF1517 0.65 -0.5 159,072 - GFF1517 0.65 +0.7 159,093 + GFF1517 0.68 +0.7 159,093 + GFF1517 0.68 +0.6 159,094 - GFF1517 0.68 -0.3 159,094 - GFF1517 0.68 -0.6 159,094 - GFF1517 0.68 +0.7 159,094 - GFF1517 0.68 -0.3 159,122 + GFF1517 0.72 -1.6 159,122 + GFF1517 0.72 -0.0 159,123 - GFF1517 0.72 +0.7 159,154 + GFF1517 0.76 -0.3 159,154 + GFF1517 0.76 +1.0 159,154 + GFF1517 0.76 -1.4 159,154 + GFF1517 0.76 -1.3 159,157 - GFF1517 0.76 -1.0 159,157 - GFF1517 0.76 -0.2 159,157 - GFF1517 0.76 -2.2 159,157 - GFF1517 0.76 -0.3 159,157 - GFF1517 0.76 -1.2 159,157 - GFF1517 0.76 -0.1 159,186 + GFF1517 0.80 -1.0 159,187 - GFF1517 0.80 -1.7 159,211 - GFF1517 0.83 -1.6 159,211 - GFF1517 0.83 -1.3 159,223 + GFF1517 0.85 -2.0 159,254 + GFF1517 0.89 -0.0 159,254 + GFF1517 0.89 -1.5 159,254 + GFF1517 0.89 -1.4 159,254 + GFF1517 0.89 -1.0 159,254 + GFF1517 0.89 -0.4 159,254 + GFF1517 0.89 -1.1 159,254 + GFF1517 0.89 -0.6 159,254 + GFF1517 0.89 -0.2 159,254 + GFF1517 0.89 -0.8 159,254 + GFF1517 0.89 -0.3 159,254 + GFF1517 0.89 -0.2 159,255 - GFF1517 0.89 -1.4 159,255 - GFF1517 0.89 -1.3 159,255 - GFF1517 0.89 +1.3 159,255 - GFF1517 0.89 +0.7 159,255 - GFF1517 0.89 -0.7 159,255 - GFF1517 0.89 +0.4 159,316 + -0.6 159,316 + -0.0 159,316 + -0.6 159,319 + +0.2 159,319 + -1.3 159,319 + -0.3 159,320 - -0.2 159,320 - +0.1 159,328 + +1.0 159,328 + -1.2 159,400 - +1.3 159,455 + +1.0 159,455 + -1.3 159,475 - -0.0 159,515 + -1.3 159,537 - -1.3 159,537 - -0.4 159,537 - -2.7 159,547 + -0.6 159,547 + -0.4 159,548 - +0.6 159,549 + -1.6 159,549 + -1.1 159,550 - +1.3 159,553 + -0.1 159,553 + -1.8 159,553 + -1.0 159,553 + -0.5 159,553 + -0.3 159,553 + -1.0 159,553 + -1.4 159,554 - -0.1 159,554 - -0.6 159,554 - -0.8 159,594 - -0.4 159,715 + -0.8 159,716 - -0.6 159,754 - -0.3 159,756 + -1.0 159,757 - -0.2 159,757 - -1.0 159,777 + -0.0 159,777 + -1.3 159,777 + -1.3 159,777 + -1.1 159,868 - +0.6 159,954 + -1.8 159,954 + +0.3 159,954 + -0.7 159,954 + +0.1 160,019 + GFF1518 0.11 -1.8 160,019 + GFF1518 0.11 +0.4 160,019 + GFF1518 0.11 -0.7 160,019 + GFF1518 0.11 -0.6 160,019 + GFF1518 0.11 -0.2 160,019 + GFF1518 0.11 -1.3 160,020 - GFF1518 0.11 -0.3 160,020 - GFF1518 0.11 -1.8 160,063 + GFF1518 0.12 +0.2 160,063 + GFF1518 0.12 -0.0 160,063 + GFF1518 0.12 -0.6 160,161 + GFF1518 0.15 -0.9 160,162 - GFF1518 0.15 -1.0 160,186 + GFF1518 0.15 -0.3 160,186 + GFF1518 0.15 -1.0 160,186 + GFF1518 0.15 -0.3 160,187 - GFF1518 0.15 -1.4 160,201 - GFF1518 0.16 -1.0 160,201 - GFF1518 0.16 -0.4 160,245 + GFF1518 0.17 -1.8 160,255 + GFF1518 0.17 -0.5 160,255 + GFF1518 0.17 -0.0 160,255 + GFF1518 0.17 -0.6 160,255 + GFF1518 0.17 -0.8 160,256 - GFF1518 0.17 -1.2 160,339 - GFF1518 0.20 -2.5 160,339 - GFF1518 0.20 -0.5
Or see this region's nucleotide sequence