Experiment: Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2026 and GFF2027 are separated by 277 nucleotides GFF2027 and GFF2028 are separated by 16 nucleotides GFF2028 and GFF2029 are separated by 24 nucleotides
GFF2026 - hypothetical protein, at 64,818 to 66,173
GFF2026
GFF2027 - hypothetical protein, at 66,451 to 66,933
GFF2027
GFF2028 - Adenine DNA glycosylase, at 66,950 to 67,993
GFF2028
GFF2029 - hypothetical protein, at 68,018 to 70,708
GFF2029
Position (kb) 66
67
68 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 66.289 kb on - strand at 66.289 kb on - strand at 66.359 kb on - strand at 66.359 kb on - strand at 66.359 kb on - strand at 66.359 kb on - strand at 66.360 kb on + strand at 66.361 kb on - strand at 66.376 kb on + strand at 66.376 kb on + strand at 66.377 kb on - strand at 66.377 kb on - strand at 66.398 kb on + strand at 66.422 kb on + strand at 66.423 kb on - strand at 66.470 kb on + strand at 66.470 kb on + strand at 66.470 kb on + strand at 66.471 kb on - strand at 66.471 kb on - strand at 66.474 kb on + strand at 66.475 kb on - strand at 66.475 kb on - strand at 66.475 kb on - strand at 66.475 kb on - strand at 66.540 kb on + strand, within GFF2027 at 66.576 kb on + strand, within GFF2027 at 66.576 kb on + strand, within GFF2027 at 66.577 kb on - strand, within GFF2027 at 66.577 kb on - strand, within GFF2027 at 66.577 kb on - strand, within GFF2027 at 66.671 kb on + strand, within GFF2027 at 66.709 kb on + strand, within GFF2027 at 66.711 kb on + strand, within GFF2027 at 66.711 kb on + strand, within GFF2027 at 66.711 kb on + strand, within GFF2027 at 66.711 kb on + strand, within GFF2027 at 66.711 kb on + strand, within GFF2027 at 66.711 kb on + strand, within GFF2027 at 66.712 kb on - strand, within GFF2027 at 66.712 kb on - strand, within GFF2027 at 66.734 kb on - strand, within GFF2027 at 66.734 kb on - strand, within GFF2027 at 66.783 kb on + strand, within GFF2027 at 66.784 kb on - strand, within GFF2027 at 66.788 kb on + strand, within GFF2027 at 66.789 kb on - strand, within GFF2027 at 66.789 kb on - strand, within GFF2027 at 66.843 kb on + strand, within GFF2027 at 66.843 kb on + strand, within GFF2027 at 66.844 kb on - strand, within GFF2027 at 66.844 kb on - strand, within GFF2027 at 66.869 kb on + strand, within GFF2027 at 66.869 kb on + strand, within GFF2027 at 66.920 kb on + strand at 66.931 kb on + strand at 66.932 kb on - strand at 66.932 kb on - strand at 66.932 kb on - strand at 66.945 kb on - strand at 66.951 kb on + strand at 66.951 kb on + strand at 66.959 kb on + strand at 66.959 kb on + strand at 66.960 kb on - strand at 66.960 kb on - strand at 66.989 kb on + strand at 67.076 kb on + strand, within GFF2028 at 67.076 kb on + strand, within GFF2028 at 67.076 kb on + strand, within GFF2028 at 67.076 kb on + strand, within GFF2028 at 67.076 kb on + strand, within GFF2028 at 67.077 kb on - strand, within GFF2028 at 67.095 kb on - strand, within GFF2028 at 67.114 kb on + strand, within GFF2028 at 67.114 kb on + strand, within GFF2028 at 67.115 kb on - strand, within GFF2028 at 67.115 kb on - strand, within GFF2028 at 67.115 kb on - strand, within GFF2028 at 67.120 kb on + strand, within GFF2028 at 67.120 kb on + strand, within GFF2028 at 67.120 kb on + strand, within GFF2028 at 67.120 kb on + strand, within GFF2028 at 67.120 kb on + strand, within GFF2028 at 67.172 kb on - strand, within GFF2028 at 67.178 kb on + strand, within GFF2028 at 67.178 kb on + strand, within GFF2028 at 67.178 kb on + strand, within GFF2028 at 67.178 kb on + strand, within GFF2028 at 67.179 kb on - strand, within GFF2028 at 67.179 kb on - strand, within GFF2028 at 67.239 kb on - strand, within GFF2028 at 67.239 kb on - strand, within GFF2028 at 67.239 kb on - strand, within GFF2028 at 67.240 kb on + strand, within GFF2028 at 67.240 kb on + strand, within GFF2028 at 67.240 kb on + strand, within GFF2028 at 67.240 kb on + strand, within GFF2028 at 67.240 kb on + strand, within GFF2028 at 67.240 kb on + strand, within GFF2028 at 67.240 kb on + strand, within GFF2028 at 67.240 kb on + strand, within GFF2028 at 67.240 kb on + strand, within GFF2028 at 67.240 kb on + strand, within GFF2028 at 67.240 kb on + strand, within GFF2028 at 67.240 kb on + strand, within GFF2028 at 67.240 kb on + strand, within GFF2028 at 67.240 kb on + strand, within GFF2028 at 67.240 kb on + strand, within GFF2028 at 67.240 kb on + strand, within GFF2028 at 67.241 kb on - strand, within GFF2028 at 67.241 kb on - strand, within GFF2028 at 67.241 kb on - strand, within GFF2028 at 67.241 kb on - strand, within GFF2028 at 67.241 kb on - strand, within GFF2028 at 67.241 kb on - strand, within GFF2028 at 67.241 kb on - strand, within GFF2028 at 67.241 kb on - strand, within GFF2028 at 67.241 kb on - strand, within GFF2028 at 67.241 kb on - strand, within GFF2028 at 67.241 kb on - strand, within GFF2028 at 67.241 kb on - strand, within GFF2028 at 67.241 kb on - strand, within GFF2028 at 67.241 kb on - strand, within GFF2028 at 67.241 kb on - strand, within GFF2028 at 67.241 kb on - strand, within GFF2028 at 67.241 kb on - strand, within GFF2028 at 67.296 kb on - strand, within GFF2028 at 67.324 kb on + strand, within GFF2028 at 67.325 kb on - strand, within GFF2028 at 67.369 kb on - strand, within GFF2028 at 67.417 kb on + strand, within GFF2028 at 67.418 kb on - strand, within GFF2028 at 67.423 kb on + strand, within GFF2028 at 67.423 kb on + strand, within GFF2028 at 67.461 kb on + strand, within GFF2028 at 67.462 kb on - strand, within GFF2028 at 67.462 kb on - strand, within GFF2028 at 67.520 kb on + strand, within GFF2028 at 67.520 kb on + strand, within GFF2028 at 67.521 kb on - strand, within GFF2028 at 67.521 kb on - strand, within GFF2028 at 67.521 kb on - strand, within GFF2028 at 67.521 kb on - strand, within GFF2028 at 67.554 kb on + strand, within GFF2028 at 67.555 kb on - strand, within GFF2028 at 67.555 kb on - strand, within GFF2028 at 67.555 kb on - strand, within GFF2028 at 67.555 kb on - strand, within GFF2028 at 67.568 kb on + strand, within GFF2028 at 67.568 kb on + strand, within GFF2028 at 67.569 kb on - strand, within GFF2028 at 67.610 kb on - strand, within GFF2028 at 67.658 kb on + strand, within GFF2028 at 67.711 kb on + strand, within GFF2028 at 67.712 kb on - strand, within GFF2028 at 67.752 kb on + strand, within GFF2028 at 67.752 kb on + strand, within GFF2028 at 67.752 kb on + strand, within GFF2028 at 67.752 kb on + strand, within GFF2028 at 67.752 kb on + strand, within GFF2028 at 67.752 kb on + strand, within GFF2028 at 67.755 kb on + strand, within GFF2028 at 67.755 kb on + strand, within GFF2028 at 67.756 kb on - strand, within GFF2028 at 67.805 kb on + strand, within GFF2028 at 67.805 kb on + strand, within GFF2028 at 67.806 kb on - strand, within GFF2028 at 67.845 kb on + strand, within GFF2028 at 67.845 kb on + strand, within GFF2028 at 67.845 kb on + strand, within GFF2028 at 67.845 kb on + strand, within GFF2028 at 67.846 kb on - strand, within GFF2028 at 67.846 kb on - strand, within GFF2028 at 67.846 kb on - strand, within GFF2028 at 67.846 kb on - strand, within GFF2028 at 67.846 kb on - strand, within GFF2028 at 67.848 kb on + strand, within GFF2028 at 67.848 kb on + strand, within GFF2028 at 67.848 kb on + strand, within GFF2028 at 67.848 kb on + strand, within GFF2028 at 67.849 kb on - strand, within GFF2028 at 67.849 kb on - strand, within GFF2028 at 67.849 kb on - strand, within GFF2028 at 67.849 kb on - strand, within GFF2028 at 67.849 kb on - strand, within GFF2028 at 67.849 kb on - strand, within GFF2028 at 67.849 kb on - strand, within GFF2028 at 67.903 kb on + strand at 67.903 kb on + strand at 67.903 kb on + strand at 67.904 kb on - strand at 67.909 kb on - strand at 67.997 kb on + strand at 67.997 kb on + strand at 68.086 kb on + strand at 68.086 kb on + strand at 68.087 kb on - strand at 68.087 kb on - strand at 68.087 kb on - strand at 68.087 kb on - strand at 68.087 kb on - strand at 68.089 kb on + strand at 68.089 kb on + strand at 68.089 kb on + strand at 68.090 kb on - strand at 68.090 kb on - strand at 68.122 kb on + strand at 68.123 kb on - strand at 68.155 kb on + strand at 68.232 kb on - strand at 68.243 kb on - strand at 68.267 kb on - strand at 68.273 kb on - strand at 68.306 kb on + strand, within GFF2029 at 68.356 kb on + strand, within GFF2029 at 68.357 kb on - strand, within GFF2029 at 68.371 kb on - strand, within GFF2029 at 68.391 kb on - strand, within GFF2029 at 68.393 kb on - strand, within GFF2029 at 68.420 kb on - strand, within GFF2029 at 68.468 kb on + strand, within GFF2029 at 68.469 kb on - strand, within GFF2029 at 68.479 kb on + strand, within GFF2029 at 68.480 kb on - strand, within GFF2029 at 68.483 kb on - strand, within GFF2029 at 68.483 kb on - strand, within GFF2029 at 68.485 kb on + strand, within GFF2029 at 68.485 kb on + strand, within GFF2029 at 68.506 kb on + strand, within GFF2029 at 68.522 kb on - strand, within GFF2029 at 68.528 kb on - strand, within GFF2029 at 68.528 kb on - strand, within GFF2029 at 68.528 kb on - strand, within GFF2029 at 68.587 kb on + strand, within GFF2029 at 68.587 kb on + strand, within GFF2029 at 68.633 kb on + strand, within GFF2029 at 68.677 kb on + strand, within GFF2029 at 68.723 kb on + strand, within GFF2029 at 68.724 kb on - strand, within GFF2029 at 68.724 kb on - strand, within GFF2029 at 68.724 kb on - strand, within GFF2029 at 68.724 kb on - strand, within GFF2029 at 68.750 kb on + strand, within GFF2029 at 68.789 kb on + strand, within GFF2029 at 68.790 kb on - strand, within GFF2029 at 68.850 kb on + strand, within GFF2029 at 68.850 kb on + strand, within GFF2029 at 68.851 kb on - strand, within GFF2029 at 68.851 kb on - strand, within GFF2029 at 68.909 kb on - strand, within GFF2029 at 68.938 kb on - strand, within GFF2029
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 1x remove 66,289 - -0.3 66,289 - -0.7 66,359 - -0.5 66,359 - -1.7 66,359 - +0.3 66,359 - -0.9 66,360 + +0.4 66,361 - -1.3 66,376 + -2.9 66,376 + -1.5 66,377 - -1.3 66,377 - -0.9 66,398 + +0.1 66,422 + -0.7 66,423 - -1.3 66,470 + -0.9 66,470 + -1.8 66,470 + -0.6 66,471 - -0.5 66,471 - +0.1 66,474 + -1.1 66,475 - -1.3 66,475 - -0.5 66,475 - -0.5 66,475 - -0.4 66,540 + GFF2027 0.18 +0.7 66,576 + GFF2027 0.26 +0.5 66,576 + GFF2027 0.26 -0.9 66,577 - GFF2027 0.26 -0.3 66,577 - GFF2027 0.26 +1.1 66,577 - GFF2027 0.26 -0.5 66,671 + GFF2027 0.46 -0.3 66,709 + GFF2027 0.53 -1.3 66,711 + GFF2027 0.54 +0.5 66,711 + GFF2027 0.54 -0.3 66,711 + GFF2027 0.54 -0.3 66,711 + GFF2027 0.54 -2.3 66,711 + GFF2027 0.54 -0.9 66,711 + GFF2027 0.54 -0.3 66,712 - GFF2027 0.54 -1.4 66,712 - GFF2027 0.54 +0.8 66,734 - GFF2027 0.59 -0.3 66,734 - GFF2027 0.59 -0.2 66,783 + GFF2027 0.69 -0.9 66,784 - GFF2027 0.69 -0.9 66,788 + GFF2027 0.70 -1.5 66,789 - GFF2027 0.70 -0.9 66,789 - GFF2027 0.70 +1.5 66,843 + GFF2027 0.81 -0.7 66,843 + GFF2027 0.81 -1.5 66,844 - GFF2027 0.81 +0.1 66,844 - GFF2027 0.81 +0.1 66,869 + GFF2027 0.87 -0.3 66,869 + GFF2027 0.87 +0.7 66,920 + -2.1 66,931 + -0.2 66,932 - -1.1 66,932 - -0.5 66,932 - -1.3 66,945 - +0.1 66,951 + -0.5 66,951 + -0.5 66,959 + -0.8 66,959 + -0.6 66,960 - -0.1 66,960 - -0.5 66,989 + -0.9 67,076 + GFF2028 0.12 +0.9 67,076 + GFF2028 0.12 -1.7 67,076 + GFF2028 0.12 -0.7 67,076 + GFF2028 0.12 -1.6 67,076 + GFF2028 0.12 -1.7 67,077 - GFF2028 0.12 -2.8 67,095 - GFF2028 0.14 +0.1 67,114 + GFF2028 0.16 -0.2 67,114 + GFF2028 0.16 +0.5 67,115 - GFF2028 0.16 +1.1 67,115 - GFF2028 0.16 -2.7 67,115 - GFF2028 0.16 +0.8 67,120 + GFF2028 0.16 -0.3 67,120 + GFF2028 0.16 +0.4 67,120 + GFF2028 0.16 -0.6 67,120 + GFF2028 0.16 -0.4 67,120 + GFF2028 0.16 -0.4 67,172 - GFF2028 0.21 -0.3 67,178 + GFF2028 0.22 -0.7 67,178 + GFF2028 0.22 -0.1 67,178 + GFF2028 0.22 +0.5 67,178 + GFF2028 0.22 -1.1 67,179 - GFF2028 0.22 -1.6 67,179 - GFF2028 0.22 -0.7 67,239 - GFF2028 0.28 +0.1 67,239 - GFF2028 0.28 -0.4 67,239 - GFF2028 0.28 +0.1 67,240 + GFF2028 0.28 +0.9 67,240 + GFF2028 0.28 -0.9 67,240 + GFF2028 0.28 -0.5 67,240 + GFF2028 0.28 +0.0 67,240 + GFF2028 0.28 +0.6 67,240 + GFF2028 0.28 -0.2 67,240 + GFF2028 0.28 -0.7 67,240 + GFF2028 0.28 +0.2 67,240 + GFF2028 0.28 -0.7 67,240 + GFF2028 0.28 -0.5 67,240 + GFF2028 0.28 -1.1 67,240 + GFF2028 0.28 -0.9 67,240 + GFF2028 0.28 +1.1 67,240 + GFF2028 0.28 +0.1 67,240 + GFF2028 0.28 -0.4 67,240 + GFF2028 0.28 -0.3 67,241 - GFF2028 0.28 +0.3 67,241 - GFF2028 0.28 +0.7 67,241 - GFF2028 0.28 -0.9 67,241 - GFF2028 0.28 -0.9 67,241 - GFF2028 0.28 +0.1 67,241 - GFF2028 0.28 -0.9 67,241 - GFF2028 0.28 +0.3 67,241 - GFF2028 0.28 +0.2 67,241 - GFF2028 0.28 +0.7 67,241 - GFF2028 0.28 -1.1 67,241 - GFF2028 0.28 -0.3 67,241 - GFF2028 0.28 -1.7 67,241 - GFF2028 0.28 -0.0 67,241 - GFF2028 0.28 +0.2 67,241 - GFF2028 0.28 -0.9 67,241 - GFF2028 0.28 -0.7 67,241 - GFF2028 0.28 +0.8 67,296 - GFF2028 0.33 -1.4 67,324 + GFF2028 0.36 -0.9 67,325 - GFF2028 0.36 -0.6 67,369 - GFF2028 0.40 -1.5 67,417 + GFF2028 0.45 -0.5 67,418 - GFF2028 0.45 -1.4 67,423 + GFF2028 0.45 -2.7 67,423 + GFF2028 0.45 -1.9 67,461 + GFF2028 0.49 -1.7 67,462 - GFF2028 0.49 -3.2 67,462 - GFF2028 0.49 -0.5 67,520 + GFF2028 0.55 -1.3 67,520 + GFF2028 0.55 -1.5 67,521 - GFF2028 0.55 -0.9 67,521 - GFF2028 0.55 -0.3 67,521 - GFF2028 0.55 -1.3 67,521 - GFF2028 0.55 -0.5 67,554 + GFF2028 0.58 -0.9 67,555 - GFF2028 0.58 -0.4 67,555 - GFF2028 0.58 -0.9 67,555 - GFF2028 0.58 -0.7 67,555 - GFF2028 0.58 +0.6 67,568 + GFF2028 0.59 -1.6 67,568 + GFF2028 0.59 +0.1 67,569 - GFF2028 0.59 -0.9 67,610 - GFF2028 0.63 -0.4 67,658 + GFF2028 0.68 -0.3 67,711 + GFF2028 0.73 +0.8 67,712 - GFF2028 0.73 -0.1 67,752 + GFF2028 0.77 -0.8 67,752 + GFF2028 0.77 -0.5 67,752 + GFF2028 0.77 -1.3 67,752 + GFF2028 0.77 -0.4 67,752 + GFF2028 0.77 -0.3 67,752 + GFF2028 0.77 -0.3 67,755 + GFF2028 0.77 -1.0 67,755 + GFF2028 0.77 -0.4 67,756 - GFF2028 0.77 +1.5 67,805 + GFF2028 0.82 -0.5 67,805 + GFF2028 0.82 -0.3 67,806 - GFF2028 0.82 -0.7 67,845 + GFF2028 0.86 -0.3 67,845 + GFF2028 0.86 +1.4 67,845 + GFF2028 0.86 +0.2 67,845 + GFF2028 0.86 -0.0 67,846 - GFF2028 0.86 -1.0 67,846 - GFF2028 0.86 -0.7 67,846 - GFF2028 0.86 -0.9 67,846 - GFF2028 0.86 -0.9 67,846 - GFF2028 0.86 +0.4 67,848 + GFF2028 0.86 +1.7 67,848 + GFF2028 0.86 -0.6 67,848 + GFF2028 0.86 +0.2 67,848 + GFF2028 0.86 +0.3 67,849 - GFF2028 0.86 -0.5 67,849 - GFF2028 0.86 -0.8 67,849 - GFF2028 0.86 -0.1 67,849 - GFF2028 0.86 -0.7 67,849 - GFF2028 0.86 -0.8 67,849 - GFF2028 0.86 -0.6 67,849 - GFF2028 0.86 -0.5 67,903 + +1.4 67,903 + -0.8 67,903 + -0.9 67,904 - -0.8 67,909 - -1.0 67,997 + -1.3 67,997 + -0.3 68,086 + +0.5 68,086 + -0.2 68,087 - -0.5 68,087 - -0.9 68,087 - -0.7 68,087 - -0.3 68,087 - -2.4 68,089 + -2.1 68,089 + -2.8 68,089 + -1.1 68,090 - -0.8 68,090 - +1.1 68,122 + -1.9 68,123 - -0.9 68,155 + -0.7 68,232 - +0.3 68,243 - -0.6 68,267 - -1.9 68,273 - -0.9 68,306 + GFF2029 0.11 -0.4 68,356 + GFF2029 0.13 -1.3 68,357 - GFF2029 0.13 -2.3 68,371 - GFF2029 0.13 -2.1 68,391 - GFF2029 0.14 -1.5 68,393 - GFF2029 0.14 -0.9 68,420 - GFF2029 0.15 -1.1 68,468 + GFF2029 0.17 -1.7 68,469 - GFF2029 0.17 +0.7 68,479 + GFF2029 0.17 -0.9 68,480 - GFF2029 0.17 -0.3 68,483 - GFF2029 0.17 -0.1 68,483 - GFF2029 0.17 -0.9 68,485 + GFF2029 0.17 -1.1 68,485 + GFF2029 0.17 +0.2 68,506 + GFF2029 0.18 -1.3 68,522 - GFF2029 0.19 -0.9 68,528 - GFF2029 0.19 -1.7 68,528 - GFF2029 0.19 +0.1 68,528 - GFF2029 0.19 -1.7 68,587 + GFF2029 0.21 +0.7 68,587 + GFF2029 0.21 -1.5 68,633 + GFF2029 0.23 -1.3 68,677 + GFF2029 0.24 -0.3 68,723 + GFF2029 0.26 -0.2 68,724 - GFF2029 0.26 -2.9 68,724 - GFF2029 0.26 -0.5 68,724 - GFF2029 0.26 -1.0 68,724 - GFF2029 0.26 -0.2 68,750 + GFF2029 0.27 -1.9 68,789 + GFF2029 0.29 -1.7 68,790 - GFF2029 0.29 -1.3 68,850 + GFF2029 0.31 -0.1 68,850 + GFF2029 0.31 +1.1 68,851 - GFF2029 0.31 -1.3 68,851 - GFF2029 0.31 -1.7 68,909 - GFF2029 0.33 -1.1 68,938 - GFF2029 0.34 -2.1
Or see this region's nucleotide sequence