Experiment: Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2005 and GFF2006 are separated by 239 nucleotides GFF2006 and GFF2007 are separated by 4 nucleotides GFF2007 and GFF2008 are separated by 78 nucleotides
GFF2005 - hypothetical protein, at 41,517 to 42,239
GFF2005
GFF2006 - Plasmid-derived single-stranded DNA-binding protein, at 42,479 to 43,009
GFF2006
GFF2007 - Inner membrane transport protein YajR, at 43,014 to 44,411
GFF2007
GFF2008 - UvrABC system protein A, at 44,490 to 47,318
GFF2008
Position (kb) 43
44
45 Strain fitness (log2 ratio) -2
-1
0
1 at 42.114 kb on + strand, within GFF2005 at 42.115 kb on - strand, within GFF2005 at 42.138 kb on + strand, within GFF2005 at 42.145 kb on + strand, within GFF2005 at 42.145 kb on + strand, within GFF2005 at 42.145 kb on + strand, within GFF2005 at 42.145 kb on + strand, within GFF2005 at 42.145 kb on + strand, within GFF2005 at 42.146 kb on - strand, within GFF2005 at 42.146 kb on - strand, within GFF2005 at 42.146 kb on - strand, within GFF2005 at 42.146 kb on - strand, within GFF2005 at 42.146 kb on - strand, within GFF2005 at 42.164 kb on - strand, within GFF2005 at 42.247 kb on + strand at 42.247 kb on + strand at 42.247 kb on + strand at 42.247 kb on + strand at 42.247 kb on + strand at 42.247 kb on + strand at 42.247 kb on + strand at 42.252 kb on + strand at 42.253 kb on - strand at 42.281 kb on + strand at 42.281 kb on + strand at 42.281 kb on + strand at 42.281 kb on + strand at 42.281 kb on + strand at 42.282 kb on - strand at 42.282 kb on - strand at 42.282 kb on - strand at 42.282 kb on - strand at 42.282 kb on - strand at 42.282 kb on - strand at 42.282 kb on - strand at 42.284 kb on + strand at 42.284 kb on + strand at 42.284 kb on + strand at 42.285 kb on - strand at 42.285 kb on - strand at 42.285 kb on - strand at 42.291 kb on + strand at 42.291 kb on + strand at 42.291 kb on + strand at 42.291 kb on + strand at 42.292 kb on - strand at 42.292 kb on - strand at 42.292 kb on - strand at 42.292 kb on - strand at 42.292 kb on - strand at 42.292 kb on - strand at 42.292 kb on - strand at 42.338 kb on - strand at 42.338 kb on - strand at 42.354 kb on - strand at 42.368 kb on + strand at 42.368 kb on + strand at 42.368 kb on + strand at 42.368 kb on + strand at 42.368 kb on + strand at 42.368 kb on + strand at 42.369 kb on - strand at 42.369 kb on - strand at 42.372 kb on - strand at 42.372 kb on - strand at 42.378 kb on + strand at 42.378 kb on + strand at 42.378 kb on + strand at 42.378 kb on + strand at 42.378 kb on + strand at 42.378 kb on + strand at 42.379 kb on - strand at 42.379 kb on - strand at 42.379 kb on - strand at 42.379 kb on - strand at 42.379 kb on - strand at 42.394 kb on + strand at 42.394 kb on + strand at 42.394 kb on + strand at 42.401 kb on + strand at 42.401 kb on + strand at 42.401 kb on + strand at 42.402 kb on - strand at 42.402 kb on - strand at 42.430 kb on + strand at 42.448 kb on + strand at 42.449 kb on - strand at 42.449 kb on - strand at 42.451 kb on + strand at 42.451 kb on + strand at 42.451 kb on + strand at 42.452 kb on - strand at 42.452 kb on - strand at 42.452 kb on - strand at 42.452 kb on - strand at 42.456 kb on + strand at 42.457 kb on - strand at 42.457 kb on - strand at 42.457 kb on - strand at 42.463 kb on + strand at 42.463 kb on + strand at 42.463 kb on + strand at 42.464 kb on - strand at 42.469 kb on + strand at 42.469 kb on + strand at 42.469 kb on + strand at 42.469 kb on + strand at 42.470 kb on - strand at 42.470 kb on - strand at 42.470 kb on - strand at 42.470 kb on - strand at 42.470 kb on - strand at 42.470 kb on - strand at 42.470 kb on - strand at 42.475 kb on + strand at 42.475 kb on + strand at 42.476 kb on - strand at 42.476 kb on - strand at 43.132 kb on + strand at 43.155 kb on - strand, within GFF2007 at 43.155 kb on - strand, within GFF2007 at 43.187 kb on + strand, within GFF2007 at 43.188 kb on - strand, within GFF2007 at 43.188 kb on - strand, within GFF2007 at 43.188 kb on - strand, within GFF2007 at 43.188 kb on - strand, within GFF2007 at 43.189 kb on + strand, within GFF2007 at 43.189 kb on + strand, within GFF2007 at 43.189 kb on + strand, within GFF2007 at 43.190 kb on - strand, within GFF2007 at 43.190 kb on - strand, within GFF2007 at 43.190 kb on - strand, within GFF2007 at 43.190 kb on - strand, within GFF2007 at 44.436 kb on - strand at 44.436 kb on - strand at 44.445 kb on + strand at 44.453 kb on + strand at 44.453 kb on + strand at 44.453 kb on + strand at 44.453 kb on + strand at 44.454 kb on - strand at 44.454 kb on - strand at 44.454 kb on - strand at 44.454 kb on - strand at 44.455 kb on + strand at 44.486 kb on + strand at 44.486 kb on + strand at 44.486 kb on + strand at 44.618 kb on + strand at 44.618 kb on + strand at 44.625 kb on + strand at 44.625 kb on + strand at 44.625 kb on + strand at 44.625 kb on + strand at 44.625 kb on + strand at 44.625 kb on + strand at 44.625 kb on + strand at 44.625 kb on + strand at 44.625 kb on + strand at 44.625 kb on + strand at 44.625 kb on + strand at 44.625 kb on + strand at 44.625 kb on + strand at 44.626 kb on - strand at 44.626 kb on - strand at 44.626 kb on - strand at 44.626 kb on - strand at 44.626 kb on - strand at 44.626 kb on - strand at 44.646 kb on + strand at 44.647 kb on - strand at 44.659 kb on + strand at 44.667 kb on + strand at 44.668 kb on + strand at 44.668 kb on - strand at 44.668 kb on - strand at 44.668 kb on - strand at 44.808 kb on + strand, within GFF2008 at 44.808 kb on + strand, within GFF2008 at 44.809 kb on - strand, within GFF2008 at 44.809 kb on - strand, within GFF2008 at 44.809 kb on - strand, within GFF2008 at 44.809 kb on - strand, within GFF2008 at 44.809 kb on - strand, within GFF2008 at 44.823 kb on + strand, within GFF2008 at 44.823 kb on + strand, within GFF2008 at 44.823 kb on + strand, within GFF2008 at 44.823 kb on + strand, within GFF2008 at 44.823 kb on + strand, within GFF2008 at 44.823 kb on + strand, within GFF2008 at 44.824 kb on - strand, within GFF2008 at 44.958 kb on - strand, within GFF2008 at 45.039 kb on + strand, within GFF2008 at 45.039 kb on + strand, within GFF2008 at 45.088 kb on + strand, within GFF2008 at 45.089 kb on - strand, within GFF2008 at 45.110 kb on + strand, within GFF2008 at 45.111 kb on - strand, within GFF2008 at 45.111 kb on - strand, within GFF2008 at 45.127 kb on + strand, within GFF2008 at 45.128 kb on - strand, within GFF2008 at 45.255 kb on + strand, within GFF2008 at 45.256 kb on - strand, within GFF2008 at 45.256 kb on - strand, within GFF2008 at 45.283 kb on + strand, within GFF2008 at 45.293 kb on + strand, within GFF2008 at 45.382 kb on + strand, within GFF2008 at 45.382 kb on + strand, within GFF2008 at 45.382 kb on + strand, within GFF2008 at 45.382 kb on + strand, within GFF2008
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 1x remove 42,114 + GFF2005 0.83 -0.5 42,115 - GFF2005 0.83 -0.9 42,138 + GFF2005 0.86 -0.2 42,145 + GFF2005 0.87 +0.1 42,145 + GFF2005 0.87 -2.4 42,145 + GFF2005 0.87 -1.4 42,145 + GFF2005 0.87 -1.9 42,145 + GFF2005 0.87 -1.3 42,146 - GFF2005 0.87 -1.6 42,146 - GFF2005 0.87 +0.1 42,146 - GFF2005 0.87 -1.6 42,146 - GFF2005 0.87 -0.5 42,146 - GFF2005 0.87 -1.7 42,164 - GFF2005 0.89 -2.1 42,247 + -1.1 42,247 + -0.5 42,247 + +0.1 42,247 + -0.3 42,247 + -1.3 42,247 + -0.9 42,247 + +1.2 42,252 + -0.5 42,253 - +0.3 42,281 + -1.9 42,281 + +0.8 42,281 + -1.5 42,281 + +0.1 42,281 + -1.4 42,282 - -0.2 42,282 - +0.7 42,282 - -1.9 42,282 - -0.1 42,282 - -1.7 42,282 - +0.7 42,282 - -0.4 42,284 + +0.7 42,284 + -2.5 42,284 + -0.8 42,285 - -1.1 42,285 - +0.1 42,285 - -0.9 42,291 + -0.3 42,291 + -2.5 42,291 + +0.7 42,291 + -1.1 42,292 - -0.9 42,292 - -0.3 42,292 - -1.3 42,292 - +0.7 42,292 - -0.7 42,292 - -1.0 42,292 - -0.1 42,338 - -0.2 42,338 - +0.1 42,354 - -0.7 42,368 + -0.2 42,368 + +0.7 42,368 + -0.9 42,368 + +0.7 42,368 + -0.6 42,368 + +0.7 42,369 - -1.5 42,369 - +0.1 42,372 - +0.3 42,372 - +0.5 42,378 + +0.1 42,378 + -2.5 42,378 + -0.4 42,378 + +0.1 42,378 + +0.7 42,378 + -1.9 42,379 - +0.1 42,379 - +0.7 42,379 - +1.1 42,379 - -0.9 42,379 - -2.3 42,394 + -0.8 42,394 + +0.4 42,394 + -0.1 42,401 + -0.7 42,401 + -0.3 42,401 + +0.7 42,402 - -1.4 42,402 - +0.5 42,430 + -0.1 42,448 + -0.4 42,449 - -1.0 42,449 - -0.9 42,451 + -1.5 42,451 + -0.0 42,451 + -0.2 42,452 - -1.0 42,452 - -1.1 42,452 - +0.1 42,452 - -1.1 42,456 + -1.6 42,457 - +0.0 42,457 - -0.3 42,457 - -0.5 42,463 + -1.9 42,463 + -0.3 42,463 + -0.5 42,464 - -0.3 42,469 + -0.3 42,469 + -1.5 42,469 + +0.1 42,469 + +1.1 42,470 - +0.9 42,470 - -2.5 42,470 - -0.0 42,470 - -1.0 42,470 - -1.5 42,470 - -1.3 42,470 - -0.1 42,475 + +0.1 42,475 + +0.1 42,476 - -0.4 42,476 - -0.1 43,132 + -1.5 43,155 - GFF2007 0.10 -1.9 43,155 - GFF2007 0.10 -0.4 43,187 + GFF2007 0.12 -0.5 43,188 - GFF2007 0.12 -2.4 43,188 - GFF2007 0.12 -0.9 43,188 - GFF2007 0.12 -0.3 43,188 - GFF2007 0.12 -1.5 43,189 + GFF2007 0.13 -1.9 43,189 + GFF2007 0.13 +0.4 43,189 + GFF2007 0.13 -0.5 43,190 - GFF2007 0.13 +0.5 43,190 - GFF2007 0.13 -0.2 43,190 - GFF2007 0.13 -0.0 43,190 - GFF2007 0.13 +1.3 44,436 - -2.0 44,436 - -0.8 44,445 + +0.5 44,453 + -1.3 44,453 + -0.7 44,453 + +0.2 44,453 + +0.1 44,454 - +0.3 44,454 - -0.5 44,454 - -0.9 44,454 - -1.3 44,455 + -1.4 44,486 + -0.3 44,486 + -0.2 44,486 + -2.1 44,618 + -1.0 44,618 + -1.3 44,625 + +0.7 44,625 + +0.1 44,625 + -1.5 44,625 + +0.0 44,625 + -0.5 44,625 + -1.1 44,625 + -1.0 44,625 + -1.3 44,625 + -0.9 44,625 + -0.3 44,625 + +0.3 44,625 + -0.3 44,625 + -0.7 44,626 - -0.9 44,626 - -1.9 44,626 - -0.3 44,626 - +0.0 44,626 - -0.2 44,626 - -1.4 44,646 + -0.9 44,647 - -0.7 44,659 + -0.9 44,667 + -0.6 44,668 + -0.1 44,668 - -0.5 44,668 - +0.3 44,668 - -0.9 44,808 + GFF2008 0.11 -1.0 44,808 + GFF2008 0.11 -0.6 44,809 - GFF2008 0.11 +1.1 44,809 - GFF2008 0.11 -0.9 44,809 - GFF2008 0.11 -1.6 44,809 - GFF2008 0.11 -0.5 44,809 - GFF2008 0.11 -1.5 44,823 + GFF2008 0.12 -0.3 44,823 + GFF2008 0.12 -0.3 44,823 + GFF2008 0.12 -0.3 44,823 + GFF2008 0.12 -0.7 44,823 + GFF2008 0.12 +1.2 44,823 + GFF2008 0.12 -0.1 44,824 - GFF2008 0.12 -0.5 44,958 - GFF2008 0.17 -1.0 45,039 + GFF2008 0.19 -0.6 45,039 + GFF2008 0.19 +0.8 45,088 + GFF2008 0.21 -0.7 45,089 - GFF2008 0.21 -0.8 45,110 + GFF2008 0.22 -0.9 45,111 - GFF2008 0.22 +0.1 45,111 - GFF2008 0.22 -0.6 45,127 + GFF2008 0.23 -1.1 45,128 - GFF2008 0.23 -0.9 45,255 + GFF2008 0.27 -0.5 45,256 - GFF2008 0.27 -2.3 45,256 - GFF2008 0.27 +0.7 45,283 + GFF2008 0.28 -1.9 45,293 + GFF2008 0.28 -0.3 45,382 + GFF2008 0.32 -1.0 45,382 + GFF2008 0.32 -0.8 45,382 + GFF2008 0.32 -0.6 45,382 + GFF2008 0.32 +0.2
Or see this region's nucleotide sequence