Strain Fitness in Methylophilus sp. DMC18 around GFF435

Experiment: Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 1x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF433 and GFF434 are separated by 27 nucleotidesGFF434 and GFF435 are separated by 152 nucleotidesGFF435 and GFF436 are separated by 200 nucleotidesGFF436 and GFF437 are separated by 98 nucleotides GFF433 - Cysteine desulfurase NifS, at 474,298 to 475,443 GFF433 GFF434 - HTH-type transcriptional regulator IscR, at 475,471 to 475,941 GFF434 GFF435 - Serine acetyltransferase, at 476,094 to 476,828 GFF435 GFF436 - hypothetical protein, at 477,029 to 477,337 GFF436 GFF437 - tRNA (cytidine/uridine/adenosine-2'-O-)-methyltransferase TrmJ, at 477,436 to 478,188 GFF437 Position (kb) 476 477Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 475.553 kb on - strand, within GFF434at 475.553 kb on - strand, within GFF434at 475.584 kb on - strand, within GFF434at 475.604 kb on - strand, within GFF434at 475.616 kb on - strand, within GFF434at 475.694 kb on - strand, within GFF434at 476.002 kb on - strandat 476.002 kb on - strandat 476.002 kb on - strandat 476.002 kb on - strandat 476.004 kb on + strandat 476.004 kb on + strandat 476.005 kb on - strandat 476.031 kb on - strandat 476.038 kb on + strandat 476.038 kb on + strandat 476.038 kb on + strandat 476.038 kb on + strandat 476.038 kb on + strandat 476.039 kb on - strandat 476.039 kb on - strandat 476.039 kb on - strandat 476.095 kb on + strandat 476.267 kb on - strand, within GFF435at 476.331 kb on - strand, within GFF435at 476.998 kb on - strandat 477.011 kb on - strandat 477.158 kb on + strand, within GFF436at 477.158 kb on + strand, within GFF436at 477.158 kb on + strand, within GFF436at 477.158 kb on + strand, within GFF436at 477.158 kb on + strand, within GFF436at 477.159 kb on - strand, within GFF436at 477.159 kb on - strand, within GFF436at 477.159 kb on - strand, within GFF436at 477.159 kb on - strand, within GFF436at 477.159 kb on - strand, within GFF436at 477.159 kb on - strand, within GFF436at 477.207 kb on + strand, within GFF436at 477.208 kb on - strand, within GFF436at 477.208 kb on - strand, within GFF436at 477.208 kb on - strand, within GFF436at 477.241 kb on + strand, within GFF436at 477.241 kb on + strand, within GFF436at 477.241 kb on + strand, within GFF436at 477.241 kb on + strand, within GFF436at 477.242 kb on - strand, within GFF436at 477.242 kb on - strand, within GFF436at 477.245 kb on + strand, within GFF436at 477.245 kb on + strand, within GFF436at 477.245 kb on + strand, within GFF436at 477.246 kb on - strand, within GFF436at 477.356 kb on + strandat 477.356 kb on + strandat 477.356 kb on + strandat 477.357 kb on - strandat 477.357 kb on - strandat 477.357 kb on - strandat 477.357 kb on - strandat 477.440 kb on + strandat 477.440 kb on + strandat 477.440 kb on + strandat 477.440 kb on + strandat 477.441 kb on - strandat 477.441 kb on - strandat 477.441 kb on - strandat 477.441 kb on - strandat 477.441 kb on - strandat 477.577 kb on - strand, within GFF437at 477.577 kb on - strand, within GFF437at 477.577 kb on - strand, within GFF437at 477.578 kb on + strand, within GFF437at 477.578 kb on + strand, within GFF437at 477.579 kb on - strand, within GFF437at 477.579 kb on - strand, within GFF437at 477.579 kb on - strand, within GFF437at 477.579 kb on - strand, within GFF437at 477.606 kb on + strand, within GFF437at 477.606 kb on + strand, within GFF437at 477.606 kb on + strand, within GFF437at 477.607 kb on - strand, within GFF437at 477.607 kb on - strand, within GFF437at 477.607 kb on - strand, within GFF437at 477.607 kb on - strand, within GFF437at 477.607 kb on - strand, within GFF437at 477.615 kb on - strand, within GFF437at 477.615 kb on - strand, within GFF437at 477.704 kb on + strand, within GFF437at 477.705 kb on - strand, within GFF437at 477.705 kb on - strand, within GFF437at 477.712 kb on + strand, within GFF437at 477.713 kb on - strand, within GFF437at 477.746 kb on + strand, within GFF437at 477.747 kb on - strand, within GFF437at 477.747 kb on - strand, within GFF437at 477.747 kb on - strand, within GFF437at 477.775 kb on + strand, within GFF437at 477.775 kb on + strand, within GFF437at 477.775 kb on + strand, within GFF437at 477.775 kb on + strand, within GFF437at 477.775 kb on + strand, within GFF437at 477.776 kb on - strand, within GFF437at 477.776 kb on - strand, within GFF437at 477.776 kb on - strand, within GFF437

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Per-strain Table

Position Strand Gene LocusTag Fraction Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 1x
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475,553 - GFF434 0.17 -0.7
475,553 - GFF434 0.17 +0.2
475,584 - GFF434 0.24 -2.1
475,604 - GFF434 0.28 +0.2
475,616 - GFF434 0.31 -0.1
475,694 - GFF434 0.47 -0.8
476,002 - -1.2
476,002 - -0.6
476,002 - +0.9
476,002 - -0.9
476,004 + -0.7
476,004 + -1.4
476,005 - -2.6
476,031 - -1.1
476,038 + -3.1
476,038 + -0.9
476,038 + -1.6
476,038 + -0.9
476,038 + +1.7
476,039 - -0.7
476,039 - -1.0
476,039 - -0.0
476,095 + -0.2
476,267 - GFF435 0.24 -1.4
476,331 - GFF435 0.32 -1.1
476,998 - -0.8
477,011 - -0.3
477,158 + GFF436 0.42 -0.5
477,158 + GFF436 0.42 -0.3
477,158 + GFF436 0.42 -0.2
477,158 + GFF436 0.42 +0.1
477,158 + GFF436 0.42 -0.8
477,159 - GFF436 0.42 -0.1
477,159 - GFF436 0.42 +0.0
477,159 - GFF436 0.42 -1.1
477,159 - GFF436 0.42 -1.9
477,159 - GFF436 0.42 -0.4
477,159 - GFF436 0.42 +0.4
477,207 + GFF436 0.58 -0.4
477,208 - GFF436 0.58 +0.1
477,208 - GFF436 0.58 +0.1
477,208 - GFF436 0.58 -1.6
477,241 + GFF436 0.69 -0.8
477,241 + GFF436 0.69 -0.9
477,241 + GFF436 0.69 -1.4
477,241 + GFF436 0.69 -1.1
477,242 - GFF436 0.69 +0.1
477,242 - GFF436 0.69 +0.4
477,245 + GFF436 0.70 -1.0
477,245 + GFF436 0.70 -0.9
477,245 + GFF436 0.70 -0.5
477,246 - GFF436 0.70 -2.5
477,356 + +0.4
477,356 + -0.1
477,356 + -0.8
477,357 - -1.1
477,357 - -1.1
477,357 - -1.8
477,357 - -0.6
477,440 + -0.7
477,440 + -1.1
477,440 + -0.9
477,440 + +0.6
477,441 - -1.8
477,441 - -0.1
477,441 - -0.9
477,441 - -1.3
477,441 - -1.1
477,577 - GFF437 0.19 -2.9
477,577 - GFF437 0.19 -0.3
477,577 - GFF437 0.19 -0.5
477,578 + GFF437 0.19 -0.1
477,578 + GFF437 0.19 +1.8
477,579 - GFF437 0.19 +0.5
477,579 - GFF437 0.19 -1.0
477,579 - GFF437 0.19 -1.1
477,579 - GFF437 0.19 -0.6
477,606 + GFF437 0.23 -0.4
477,606 + GFF437 0.23 -0.3
477,606 + GFF437 0.23 +0.1
477,607 - GFF437 0.23 -0.9
477,607 - GFF437 0.23 -1.6
477,607 - GFF437 0.23 +0.4
477,607 - GFF437 0.23 +0.5
477,607 - GFF437 0.23 +0.1
477,615 - GFF437 0.24 -0.5
477,615 - GFF437 0.24 -0.5
477,704 + GFF437 0.36 -0.7
477,705 - GFF437 0.36 -1.4
477,705 - GFF437 0.36 +0.0
477,712 + GFF437 0.37 -1.4
477,713 - GFF437 0.37 -0.8
477,746 + GFF437 0.41 -1.6
477,747 - GFF437 0.41 -0.1
477,747 - GFF437 0.41 -2.1
477,747 - GFF437 0.41 +0.3
477,775 + GFF437 0.45 +0.8
477,775 + GFF437 0.45 +0.3
477,775 + GFF437 0.45 -0.1
477,775 + GFF437 0.45 -0.5
477,775 + GFF437 0.45 -0.2
477,776 - GFF437 0.45 +0.6
477,776 - GFF437 0.45 -0.9
477,776 - GFF437 0.45 -0.1

Or see this region's nucleotide sequence