Experiment: Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF345 and GFF346 are separated by 46 nucleotides GFF346 and GFF347 are separated by 60 nucleotides GFF347 and GFF348 are separated by 294 nucleotides GFF348 and GFF349 are separated by 109 nucleotides
GFF345 - hypothetical protein, at 373,761 to 374,363
GFF345
GFF346 - putative DNA endonuclease SmrA, at 374,410 to 374,955
GFF346
GFF347 - Thioredoxin reductase, at 375,016 to 375,966
GFF347
GFF348 - putative protein, at 376,261 to 376,725
GFF348
GFF349 - DNA translocase FtsK, at 376,835 to 379,150
GFF349
Position (kb) 375
376 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 374.181 kb on + strand, within GFF345 at 374.181 kb on + strand, within GFF345 at 374.182 kb on - strand, within GFF345 at 374.182 kb on - strand, within GFF345 at 374.202 kb on + strand, within GFF345 at 374.207 kb on + strand, within GFF345 at 374.278 kb on + strand, within GFF345 at 374.278 kb on + strand, within GFF345 at 374.279 kb on - strand, within GFF345 at 374.356 kb on - strand at 374.356 kb on - strand at 374.361 kb on + strand at 374.362 kb on - strand at 374.472 kb on - strand, within GFF346 at 374.509 kb on + strand, within GFF346 at 374.521 kb on - strand, within GFF346 at 374.521 kb on - strand, within GFF346 at 374.521 kb on - strand, within GFF346 at 374.535 kb on + strand, within GFF346 at 374.536 kb on - strand, within GFF346 at 374.572 kb on + strand, within GFF346 at 374.573 kb on - strand, within GFF346 at 374.607 kb on + strand, within GFF346 at 374.608 kb on - strand, within GFF346 at 374.701 kb on + strand, within GFF346 at 374.729 kb on - strand, within GFF346 at 374.732 kb on + strand, within GFF346 at 374.733 kb on - strand, within GFF346 at 374.733 kb on - strand, within GFF346 at 374.733 kb on - strand, within GFF346 at 374.737 kb on + strand, within GFF346 at 374.737 kb on + strand, within GFF346 at 374.737 kb on + strand, within GFF346 at 374.738 kb on - strand, within GFF346 at 374.762 kb on + strand, within GFF346 at 374.774 kb on + strand, within GFF346 at 374.774 kb on + strand, within GFF346 at 374.774 kb on + strand, within GFF346 at 374.775 kb on - strand, within GFF346 at 374.775 kb on - strand, within GFF346 at 374.775 kb on - strand, within GFF346 at 374.775 kb on - strand, within GFF346 at 374.775 kb on - strand, within GFF346 at 374.775 kb on - strand, within GFF346 at 374.775 kb on - strand, within GFF346 at 374.775 kb on - strand, within GFF346 at 374.775 kb on - strand, within GFF346 at 374.775 kb on - strand, within GFF346 at 374.775 kb on - strand, within GFF346 at 374.775 kb on - strand, within GFF346 at 374.906 kb on + strand at 374.907 kb on - strand at 374.955 kb on + strand at 374.956 kb on - strand at 374.956 kb on - strand at 374.956 kb on - strand at 376.025 kb on + strand at 376.026 kb on - strand at 376.026 kb on - strand at 376.055 kb on + strand at 376.055 kb on + strand at 376.055 kb on + strand at 376.055 kb on + strand at 376.056 kb on - strand at 376.056 kb on - strand at 376.056 kb on - strand at 376.056 kb on - strand at 376.056 kb on - strand at 376.056 kb on - strand at 376.057 kb on + strand at 376.057 kb on + strand at 376.057 kb on + strand at 376.057 kb on + strand at 376.057 kb on + strand at 376.057 kb on + strand at 376.057 kb on + strand at 376.057 kb on + strand at 376.057 kb on + strand at 376.057 kb on + strand at 376.057 kb on + strand at 376.057 kb on + strand at 376.058 kb on - strand at 376.058 kb on - strand at 376.058 kb on - strand at 376.058 kb on - strand at 376.058 kb on - strand at 376.058 kb on - strand at 376.058 kb on - strand at 376.058 kb on - strand at 376.058 kb on - strand at 376.058 kb on - strand at 376.058 kb on - strand at 376.058 kb on - strand at 376.058 kb on - strand at 376.058 kb on - strand at 376.059 kb on + strand at 376.059 kb on + strand at 376.059 kb on + strand at 376.060 kb on - strand at 376.083 kb on + strand at 376.108 kb on - strand at 376.121 kb on + strand at 376.122 kb on - strand at 376.131 kb on + strand at 376.131 kb on + strand at 376.132 kb on - strand at 376.146 kb on + strand at 376.146 kb on + strand at 376.147 kb on - strand at 376.147 kb on - strand at 376.147 kb on - strand at 376.147 kb on - strand at 376.168 kb on - strand at 376.168 kb on - strand at 376.172 kb on + strand at 376.173 kb on - strand at 376.183 kb on + strand at 376.183 kb on + strand at 376.183 kb on + strand at 376.184 kb on - strand at 376.184 kb on - strand at 376.184 kb on - strand at 376.184 kb on - strand at 376.184 kb on - strand at 376.188 kb on + strand at 376.188 kb on + strand at 376.188 kb on + strand at 376.188 kb on + strand at 376.188 kb on + strand at 376.188 kb on + strand at 376.189 kb on - strand at 376.189 kb on - strand at 376.189 kb on - strand at 376.189 kb on - strand at 376.189 kb on - strand at 376.189 kb on - strand at 376.189 kb on - strand at 376.189 kb on - strand at 376.192 kb on + strand at 376.192 kb on + strand at 376.193 kb on - strand at 376.193 kb on - strand at 376.193 kb on - strand at 376.235 kb on - strand at 376.239 kb on + strand at 376.239 kb on + strand at 376.240 kb on - strand at 376.240 kb on - strand at 376.240 kb on - strand at 376.240 kb on - strand at 376.240 kb on - strand at 376.282 kb on + strand at 376.377 kb on + strand, within GFF348 at 376.377 kb on + strand, within GFF348 at 376.378 kb on - strand, within GFF348 at 376.378 kb on - strand, within GFF348 at 376.378 kb on - strand, within GFF348 at 376.378 kb on - strand, within GFF348 at 376.428 kb on + strand, within GFF348 at 376.429 kb on - strand, within GFF348 at 376.429 kb on - strand, within GFF348 at 376.440 kb on + strand, within GFF348 at 376.490 kb on + strand, within GFF348 at 376.490 kb on + strand, within GFF348 at 376.491 kb on - strand, within GFF348 at 376.491 kb on - strand, within GFF348 at 376.505 kb on + strand, within GFF348 at 376.506 kb on - strand, within GFF348 at 376.506 kb on - strand, within GFF348 at 376.506 kb on - strand, within GFF348 at 376.565 kb on + strand, within GFF348 at 376.566 kb on - strand, within GFF348 at 376.566 kb on - strand, within GFF348 at 376.566 kb on - strand, within GFF348 at 376.566 kb on - strand, within GFF348 at 376.566 kb on - strand, within GFF348 at 376.593 kb on + strand, within GFF348 at 376.594 kb on - strand, within GFF348 at 376.594 kb on - strand, within GFF348 at 376.594 kb on - strand, within GFF348 at 376.594 kb on - strand, within GFF348 at 376.594 kb on - strand, within GFF348 at 376.629 kb on - strand, within GFF348 at 376.643 kb on + strand, within GFF348 at 376.643 kb on + strand, within GFF348 at 376.644 kb on - strand, within GFF348 at 376.644 kb on - strand, within GFF348 at 376.644 kb on - strand, within GFF348 at 376.644 kb on - strand, within GFF348 at 376.644 kb on - strand, within GFF348 at 376.644 kb on - strand, within GFF348 at 376.644 kb on - strand, within GFF348 at 376.645 kb on + strand, within GFF348 at 376.646 kb on - strand, within GFF348 at 376.646 kb on - strand, within GFF348 at 376.646 kb on - strand, within GFF348 at 376.646 kb on - strand, within GFF348 at 376.646 kb on - strand, within GFF348 at 376.646 kb on - strand, within GFF348 at 376.646 kb on - strand, within GFF348 at 376.652 kb on + strand, within GFF348 at 376.652 kb on + strand, within GFF348 at 376.653 kb on - strand, within GFF348 at 376.653 kb on - strand, within GFF348 at 376.653 kb on - strand, within GFF348 at 376.653 kb on - strand, within GFF348 at 376.653 kb on - strand, within GFF348 at 376.653 kb on - strand, within GFF348 at 376.710 kb on + strand at 376.711 kb on - strand at 376.746 kb on + strand at 376.747 kb on - strand at 376.747 kb on - strand at 376.751 kb on + strand at 376.752 kb on - strand at 376.752 kb on - strand at 376.756 kb on + strand at 376.756 kb on + strand at 376.756 kb on + strand at 376.757 kb on - strand at 376.795 kb on - strand at 376.803 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 1x remove 374,181 + GFF345 0.70 -2.0 374,181 + GFF345 0.70 +0.4 374,182 - GFF345 0.70 -1.0 374,182 - GFF345 0.70 -0.6 374,202 + GFF345 0.73 +0.6 374,207 + GFF345 0.74 -0.2 374,278 + GFF345 0.86 -0.7 374,278 + GFF345 0.86 -0.6 374,279 - GFF345 0.86 -0.2 374,356 - -0.0 374,356 - -0.0 374,361 + -0.2 374,362 - -0.7 374,472 - GFF346 0.11 +0.1 374,509 + GFF346 0.18 -0.3 374,521 - GFF346 0.20 -1.8 374,521 - GFF346 0.20 -0.1 374,521 - GFF346 0.20 -0.4 374,535 + GFF346 0.23 +0.6 374,536 - GFF346 0.23 -0.5 374,572 + GFF346 0.30 -2.0 374,573 - GFF346 0.30 +0.4 374,607 + GFF346 0.36 -1.4 374,608 - GFF346 0.36 -2.2 374,701 + GFF346 0.53 +0.6 374,729 - GFF346 0.58 +0.5 374,732 + GFF346 0.59 -0.5 374,733 - GFF346 0.59 +1.5 374,733 - GFF346 0.59 -0.1 374,733 - GFF346 0.59 -0.0 374,737 + GFF346 0.60 +0.2 374,737 + GFF346 0.60 +0.4 374,737 + GFF346 0.60 +0.3 374,738 - GFF346 0.60 +0.6 374,762 + GFF346 0.64 -1.8 374,774 + GFF346 0.67 -0.8 374,774 + GFF346 0.67 +0.2 374,774 + GFF346 0.67 -0.1 374,775 - GFF346 0.67 +0.1 374,775 - GFF346 0.67 +0.7 374,775 - GFF346 0.67 -0.3 374,775 - GFF346 0.67 +0.3 374,775 - GFF346 0.67 +0.5 374,775 - GFF346 0.67 -1.0 374,775 - GFF346 0.67 -1.6 374,775 - GFF346 0.67 -0.6 374,775 - GFF346 0.67 -0.6 374,775 - GFF346 0.67 -0.4 374,775 - GFF346 0.67 -1.4 374,775 - GFF346 0.67 +1.5 374,906 + -0.4 374,907 - +0.1 374,955 + +0.7 374,956 - +0.2 374,956 - -0.7 374,956 - -1.8 376,025 + -1.0 376,026 - -0.5 376,026 - -1.4 376,055 + -1.6 376,055 + -1.4 376,055 + -1.4 376,055 + +0.3 376,056 - -0.2 376,056 - -1.7 376,056 - -0.4 376,056 - -0.5 376,056 - -1.1 376,056 - -0.2 376,057 + -0.6 376,057 + -0.4 376,057 + -1.6 376,057 + +1.2 376,057 + -0.8 376,057 + -0.7 376,057 + -0.3 376,057 + -1.0 376,057 + -1.6 376,057 + -0.0 376,057 + -2.5 376,057 + -0.0 376,058 - +1.0 376,058 - -1.8 376,058 - -1.0 376,058 - +0.3 376,058 - -0.6 376,058 - -0.5 376,058 - +0.2 376,058 - -1.0 376,058 - -0.8 376,058 - -0.8 376,058 - -0.9 376,058 - -0.0 376,058 - +0.5 376,058 - -0.6 376,059 + -0.6 376,059 + -0.9 376,059 + +0.2 376,060 - -0.5 376,083 + -0.8 376,108 - +0.6 376,121 + +0.5 376,122 - -0.4 376,131 + -0.4 376,131 + -0.4 376,132 - -0.8 376,146 + -1.2 376,146 + +0.7 376,147 - +1.0 376,147 - +0.1 376,147 - +0.1 376,147 - -1.2 376,168 - -0.2 376,168 - +0.5 376,172 + +0.6 376,173 - +0.5 376,183 + -0.6 376,183 + -2.0 376,183 + -0.3 376,184 - -0.2 376,184 - -0.7 376,184 - +0.5 376,184 - +0.4 376,184 - +0.6 376,188 + -0.9 376,188 + -0.0 376,188 + +0.1 376,188 + +0.1 376,188 + -0.4 376,188 + -0.0 376,189 - -0.5 376,189 - -0.6 376,189 - -0.2 376,189 - +0.5 376,189 - -0.4 376,189 - -0.8 376,189 - -0.4 376,189 - +0.6 376,192 + -0.0 376,192 + +1.0 376,193 - -0.0 376,193 - -0.3 376,193 - -0.2 376,235 - -1.0 376,239 + -0.8 376,239 + -1.0 376,240 - -0.6 376,240 - +0.2 376,240 - -1.9 376,240 - -0.3 376,240 - -0.5 376,282 + -1.4 376,377 + GFF348 0.25 -2.3 376,377 + GFF348 0.25 -1.0 376,378 - GFF348 0.25 -0.4 376,378 - GFF348 0.25 -1.2 376,378 - GFF348 0.25 -0.7 376,378 - GFF348 0.25 -1.0 376,428 + GFF348 0.36 -2.9 376,429 - GFF348 0.36 +0.4 376,429 - GFF348 0.36 -0.4 376,440 + GFF348 0.38 -0.7 376,490 + GFF348 0.49 -0.0 376,490 + GFF348 0.49 -0.0 376,491 - GFF348 0.49 +0.7 376,491 - GFF348 0.49 -0.7 376,505 + GFF348 0.52 -1.6 376,506 - GFF348 0.53 -0.9 376,506 - GFF348 0.53 -0.1 376,506 - GFF348 0.53 -0.5 376,565 + GFF348 0.65 -0.0 376,566 - GFF348 0.66 +0.2 376,566 - GFF348 0.66 -0.9 376,566 - GFF348 0.66 -0.6 376,566 - GFF348 0.66 -1.0 376,566 - GFF348 0.66 +0.3 376,593 + GFF348 0.71 -1.4 376,594 - GFF348 0.72 -0.8 376,594 - GFF348 0.72 -0.5 376,594 - GFF348 0.72 -1.1 376,594 - GFF348 0.72 -0.8 376,594 - GFF348 0.72 +1.5 376,629 - GFF348 0.79 +0.4 376,643 + GFF348 0.82 -0.3 376,643 + GFF348 0.82 +0.2 376,644 - GFF348 0.82 -0.0 376,644 - GFF348 0.82 -0.6 376,644 - GFF348 0.82 -1.1 376,644 - GFF348 0.82 -2.2 376,644 - GFF348 0.82 -2.2 376,644 - GFF348 0.82 -0.4 376,644 - GFF348 0.82 +0.7 376,645 + GFF348 0.83 -0.4 376,646 - GFF348 0.83 -0.8 376,646 - GFF348 0.83 -1.4 376,646 - GFF348 0.83 -0.8 376,646 - GFF348 0.83 -1.5 376,646 - GFF348 0.83 -1.4 376,646 - GFF348 0.83 +1.0 376,646 - GFF348 0.83 -0.8 376,652 + GFF348 0.84 +1.2 376,652 + GFF348 0.84 -1.0 376,653 - GFF348 0.84 -1.4 376,653 - GFF348 0.84 +0.6 376,653 - GFF348 0.84 -0.0 376,653 - GFF348 0.84 -1.4 376,653 - GFF348 0.84 +0.7 376,653 - GFF348 0.84 -0.8 376,710 + -1.4 376,711 - -0.3 376,746 + -0.2 376,747 - +1.0 376,747 - -1.5 376,751 + +0.1 376,752 - +0.7 376,752 - +0.4 376,756 + -0.7 376,756 + -0.1 376,756 + -0.6 376,757 - -0.2 376,795 - -1.0 376,803 - -1.0
Or see this region's nucleotide sequence