Experiment: Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF32 and GFF33 are separated by 516 nucleotides GFF33 and GFF34 overlap by 8 nucleotides GFF34 and GFF35 are separated by 14 nucleotides
GFF32 - hypothetical protein, at 43,345 to 44,301
GFF32
GFF33 - hypothetical protein, at 44,818 to 46,215
GFF33
GFF34 - hypothetical protein, at 46,208 to 46,954
GFF34
GFF35 - Transcriptional activator protein ExaE, at 46,969 to 47,601
GFF35
Position (kb) 44
45
46
47 Strain fitness (log2 ratio) -2
-1
0
1 at 43.830 kb on + strand, within GFF32 at 43.878 kb on + strand, within GFF32 at 43.878 kb on + strand, within GFF32 at 43.878 kb on + strand, within GFF32 at 43.879 kb on - strand, within GFF32 at 43.882 kb on + strand, within GFF32 at 43.883 kb on - strand, within GFF32 at 43.950 kb on + strand, within GFF32 at 44.011 kb on + strand, within GFF32 at 44.012 kb on - strand, within GFF32 at 44.055 kb on + strand, within GFF32 at 44.056 kb on - strand, within GFF32 at 44.056 kb on - strand, within GFF32 at 44.152 kb on + strand, within GFF32 at 44.265 kb on + strand at 44.265 kb on + strand at 44.265 kb on + strand at 44.266 kb on - strand at 44.266 kb on - strand at 44.266 kb on - strand at 44.299 kb on + strand at 44.299 kb on + strand at 44.299 kb on + strand at 44.299 kb on + strand at 44.299 kb on + strand at 44.300 kb on - strand at 44.300 kb on - strand at 44.300 kb on - strand at 44.300 kb on - strand at 44.300 kb on - strand at 44.331 kb on + strand at 44.331 kb on + strand at 44.331 kb on + strand at 44.331 kb on + strand at 44.406 kb on + strand at 44.407 kb on - strand at 44.429 kb on - strand at 44.432 kb on + strand at 44.433 kb on - strand at 44.433 kb on - strand at 44.433 kb on - strand at 44.433 kb on - strand at 44.433 kb on - strand at 44.458 kb on + strand at 44.477 kb on + strand at 44.477 kb on + strand at 44.478 kb on - strand at 44.478 kb on - strand at 44.492 kb on - strand at 44.519 kb on + strand at 44.519 kb on + strand at 44.529 kb on + strand at 44.530 kb on - strand at 44.571 kb on + strand at 44.590 kb on + strand at 44.591 kb on - strand at 44.596 kb on + strand at 44.597 kb on - strand at 44.635 kb on - strand at 44.635 kb on - strand at 44.635 kb on - strand at 44.635 kb on - strand at 44.670 kb on + strand at 44.670 kb on + strand at 44.671 kb on - strand at 44.671 kb on - strand at 44.675 kb on + strand at 44.693 kb on + strand at 44.694 kb on - strand at 44.708 kb on + strand at 44.708 kb on + strand at 44.708 kb on + strand at 44.708 kb on + strand at 44.709 kb on - strand at 44.709 kb on - strand at 44.721 kb on - strand at 44.727 kb on + strand at 44.728 kb on - strand at 44.814 kb on + strand at 44.815 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 1x remove 43,830 + GFF32 0.51 -0.6 43,878 + GFF32 0.56 -1.0 43,878 + GFF32 0.56 +0.6 43,878 + GFF32 0.56 +1.6 43,879 - GFF32 0.56 -0.4 43,882 + GFF32 0.56 -1.0 43,883 - GFF32 0.56 -0.0 43,950 + GFF32 0.63 -1.7 44,011 + GFF32 0.70 -0.4 44,012 - GFF32 0.70 -1.6 44,055 + GFF32 0.74 +0.1 44,056 - GFF32 0.74 -0.3 44,056 - GFF32 0.74 -1.0 44,152 + GFF32 0.84 -1.1 44,265 + -1.3 44,265 + +1.1 44,265 + +0.1 44,266 - -0.2 44,266 - -0.5 44,266 - -0.6 44,299 + +1.6 44,299 + -1.8 44,299 + -1.2 44,299 + -2.3 44,299 + -1.0 44,300 - +0.5 44,300 - -1.5 44,300 - -0.7 44,300 - -1.1 44,300 - -0.9 44,331 + +0.6 44,331 + -0.3 44,331 + +0.4 44,331 + -0.5 44,406 + -1.3 44,407 - -1.3 44,429 - -0.5 44,432 + -1.6 44,433 - +0.3 44,433 - -0.4 44,433 - -0.8 44,433 - +0.8 44,433 - -0.6 44,458 + +0.7 44,477 + -0.5 44,477 + -0.4 44,478 - +1.0 44,478 - +0.7 44,492 - -0.3 44,519 + -1.3 44,519 + -1.3 44,529 + -0.6 44,530 - -0.6 44,571 + -0.6 44,590 + -1.0 44,591 - -0.4 44,596 + -1.8 44,597 - -0.6 44,635 - -1.0 44,635 - +0.6 44,635 - +0.2 44,635 - -0.3 44,670 + -0.6 44,670 + +0.2 44,671 - -0.4 44,671 - -0.8 44,675 + +1.0 44,693 + -1.2 44,694 - -1.3 44,708 + -0.4 44,708 + -1.0 44,708 + -1.6 44,708 + -0.3 44,709 - -1.3 44,709 - -1.2 44,721 - -0.2 44,727 + +0.6 44,728 - +0.4 44,814 + +0.6 44,815 - -1.0
Or see this region's nucleotide sequence