Experiment: Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2822 and GFF2823 are separated by 148 nucleotides GFF2823 and GFF2824 are separated by 24 nucleotides
GFF2822 - Multidrug resistance protein MexB, at 54,367 to 57,567
GFF2822
GFF2823 - Protein-L-isoaspartate O-methyltransferase, at 57,716 to 58,372
GFF2823
GFF2824 - Outer membrane protein TolC, at 58,397 to 59,743
GFF2824
Position (kb) 57
58
59 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 56.747 kb on + strand, within GFF2822 at 56.747 kb on + strand, within GFF2822 at 56.807 kb on + strand, within GFF2822 at 56.807 kb on + strand, within GFF2822 at 56.808 kb on - strand, within GFF2822 at 56.933 kb on + strand, within GFF2822 at 56.933 kb on + strand, within GFF2822 at 57.023 kb on + strand, within GFF2822 at 57.024 kb on - strand, within GFF2822 at 57.158 kb on + strand, within GFF2822 at 57.158 kb on + strand, within GFF2822 at 57.182 kb on + strand, within GFF2822 at 57.182 kb on + strand, within GFF2822 at 57.182 kb on + strand, within GFF2822 at 57.183 kb on - strand, within GFF2822 at 57.189 kb on + strand, within GFF2822 at 57.189 kb on + strand, within GFF2822 at 57.213 kb on + strand, within GFF2822 at 57.273 kb on + strand at 57.273 kb on + strand at 57.273 kb on + strand at 57.273 kb on + strand at 57.273 kb on + strand at 57.273 kb on + strand at 57.274 kb on - strand at 57.274 kb on - strand at 57.274 kb on - strand at 57.274 kb on - strand at 57.377 kb on + strand at 57.509 kb on + strand at 57.509 kb on + strand at 57.511 kb on + strand at 57.512 kb on - strand at 57.512 kb on - strand at 57.599 kb on + strand at 57.599 kb on + strand at 57.599 kb on + strand at 57.600 kb on - strand at 57.600 kb on - strand at 57.604 kb on + strand at 57.604 kb on + strand at 57.604 kb on + strand at 57.604 kb on + strand at 57.604 kb on + strand at 57.604 kb on + strand at 57.605 kb on - strand at 57.605 kb on - strand at 57.605 kb on - strand at 57.605 kb on - strand at 57.605 kb on - strand at 57.608 kb on + strand at 57.608 kb on + strand at 57.612 kb on + strand at 57.612 kb on + strand at 57.612 kb on + strand at 57.613 kb on - strand at 57.641 kb on + strand at 57.642 kb on - strand at 57.664 kb on + strand at 57.664 kb on + strand at 57.664 kb on + strand at 57.665 kb on - strand at 57.665 kb on - strand at 57.665 kb on - strand at 57.675 kb on + strand at 57.675 kb on + strand at 57.675 kb on + strand at 57.675 kb on + strand at 57.675 kb on + strand at 57.676 kb on - strand at 57.676 kb on - strand at 57.676 kb on - strand at 57.676 kb on - strand at 57.677 kb on + strand at 58.098 kb on + strand, within GFF2823 at 58.130 kb on + strand, within GFF2823 at 58.148 kb on + strand, within GFF2823 at 58.363 kb on + strand at 58.370 kb on + strand at 58.388 kb on + strand at 58.388 kb on + strand at 58.389 kb on - strand at 58.389 kb on - strand at 58.396 kb on + strand at 58.396 kb on + strand at 58.397 kb on - strand at 58.397 kb on - strand at 58.412 kb on + strand at 58.493 kb on + strand at 58.566 kb on + strand, within GFF2824 at 58.566 kb on + strand, within GFF2824 at 58.566 kb on + strand, within GFF2824 at 58.638 kb on + strand, within GFF2824 at 58.685 kb on + strand, within GFF2824 at 58.685 kb on + strand, within GFF2824 at 58.707 kb on + strand, within GFF2824 at 58.707 kb on + strand, within GFF2824 at 58.707 kb on + strand, within GFF2824 at 58.707 kb on + strand, within GFF2824 at 58.752 kb on + strand, within GFF2824 at 58.793 kb on + strand, within GFF2824 at 58.794 kb on - strand, within GFF2824 at 59.029 kb on + strand, within GFF2824 at 59.244 kb on + strand, within GFF2824 at 59.244 kb on + strand, within GFF2824 at 59.244 kb on + strand, within GFF2824 at 59.261 kb on + strand, within GFF2824 at 59.262 kb on - strand, within GFF2824 at 59.262 kb on - strand, within GFF2824 at 59.273 kb on + strand, within GFF2824 at 59.323 kb on + strand, within GFF2824 at 59.325 kb on + strand, within GFF2824 at 59.325 kb on + strand, within GFF2824 at 59.351 kb on + strand, within GFF2824 at 59.351 kb on + strand, within GFF2824
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 1x remove 56,747 + GFF2822 0.74 -0.4 56,747 + GFF2822 0.74 -1.5 56,807 + GFF2822 0.76 -1.0 56,807 + GFF2822 0.76 -1.3 56,808 - GFF2822 0.76 -0.1 56,933 + GFF2822 0.80 -0.7 56,933 + GFF2822 0.80 -0.4 57,023 + GFF2822 0.83 -1.5 57,024 - GFF2822 0.83 -1.3 57,158 + GFF2822 0.87 +0.4 57,158 + GFF2822 0.87 -1.3 57,182 + GFF2822 0.88 -1.8 57,182 + GFF2822 0.88 +0.0 57,182 + GFF2822 0.88 -1.0 57,183 - GFF2822 0.88 -1.0 57,189 + GFF2822 0.88 -1.0 57,189 + GFF2822 0.88 +0.4 57,213 + GFF2822 0.89 +0.3 57,273 + -0.7 57,273 + -1.0 57,273 + -1.3 57,273 + -0.8 57,273 + +1.0 57,273 + -1.0 57,274 - -1.0 57,274 - +0.2 57,274 - +0.3 57,274 - +1.0 57,377 + +0.3 57,509 + +0.6 57,509 + +0.3 57,511 + +0.0 57,512 - -1.3 57,512 - -2.0 57,599 + -0.3 57,599 + -0.3 57,599 + -1.0 57,600 - -1.0 57,600 - -0.3 57,604 + -1.7 57,604 + +0.4 57,604 + +0.0 57,604 + +0.0 57,604 + +0.6 57,604 + -0.1 57,605 - -1.1 57,605 - -1.9 57,605 - +0.0 57,605 - -1.1 57,605 - -0.2 57,608 + +0.5 57,608 + +0.3 57,612 + -2.8 57,612 + -0.5 57,612 + -0.5 57,613 - -0.6 57,641 + -1.3 57,642 - +0.5 57,664 + +1.5 57,664 + -0.5 57,664 + +0.3 57,665 - -1.0 57,665 - -0.8 57,665 - +1.6 57,675 + +0.4 57,675 + -0.1 57,675 + +1.0 57,675 + -0.8 57,675 + -0.3 57,676 - +0.5 57,676 - -1.8 57,676 - +0.3 57,676 - -0.3 57,677 + +0.0 58,098 + GFF2823 0.58 -1.0 58,130 + GFF2823 0.63 +0.7 58,148 + GFF2823 0.66 +0.6 58,363 + +0.0 58,370 + -2.7 58,388 + -0.8 58,388 + -1.3 58,389 - -1.8 58,389 - -1.0 58,396 + -0.5 58,396 + -2.3 58,397 - -1.2 58,397 - -1.3 58,412 + +0.6 58,493 + -1.0 58,566 + GFF2824 0.13 -1.8 58,566 + GFF2824 0.13 +0.0 58,566 + GFF2824 0.13 +0.6 58,638 + GFF2824 0.18 -1.1 58,685 + GFF2824 0.21 -1.3 58,685 + GFF2824 0.21 +0.7 58,707 + GFF2824 0.23 +1.0 58,707 + GFF2824 0.23 +1.0 58,707 + GFF2824 0.23 +1.6 58,707 + GFF2824 0.23 +0.0 58,752 + GFF2824 0.26 -0.1 58,793 + GFF2824 0.29 -0.2 58,794 - GFF2824 0.29 -0.7 59,029 + GFF2824 0.47 +0.0 59,244 + GFF2824 0.63 -1.0 59,244 + GFF2824 0.63 -0.5 59,244 + GFF2824 0.63 -0.1 59,261 + GFF2824 0.64 -2.1 59,262 - GFF2824 0.64 +1.0 59,262 - GFF2824 0.64 +0.0 59,273 + GFF2824 0.65 +0.2 59,323 + GFF2824 0.69 -1.0 59,325 + GFF2824 0.69 -0.8 59,325 + GFF2824 0.69 -1.0 59,351 + GFF2824 0.71 +0.0 59,351 + GFF2824 0.71 -0.4
Or see this region's nucleotide sequence