Experiment: Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2804 and GFF2805 are separated by 6 nucleotides GFF2805 and GFF2806 are separated by 12 nucleotides GFF2806 and GFF2807 overlap by 4 nucleotides
GFF2804 - hypothetical protein, at 37,754 to 38,683
GFF2804
GFF2805 - Sepiapterin reductase, at 38,690 to 39,481
GFF2805
GFF2806 - hypothetical protein, at 39,494 to 40,192
GFF2806
GFF2807 - Long-chain-fatty-acid--CoA ligase, at 40,189 to 41,685
GFF2807
Position (kb) 38
39
40 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 37.698 kb on + strand at 37.698 kb on + strand at 37.699 kb on - strand at 37.699 kb on - strand at 37.699 kb on - strand at 37.721 kb on + strand at 37.722 kb on - strand at 37.722 kb on - strand at 37.745 kb on + strand at 37.746 kb on - strand at 37.758 kb on + strand at 37.759 kb on - strand at 37.791 kb on + strand at 37.791 kb on + strand at 37.791 kb on + strand at 37.792 kb on - strand at 37.792 kb on - strand at 37.792 kb on - strand at 37.793 kb on + strand at 37.793 kb on + strand at 37.793 kb on + strand at 37.794 kb on - strand at 37.794 kb on - strand at 37.797 kb on + strand at 37.797 kb on + strand at 37.797 kb on + strand at 37.797 kb on + strand at 37.798 kb on - strand at 37.798 kb on - strand at 37.798 kb on - strand at 37.798 kb on - strand at 37.804 kb on - strand at 37.813 kb on + strand at 37.813 kb on + strand at 37.813 kb on + strand at 37.814 kb on - strand at 37.843 kb on + strand at 37.843 kb on + strand at 37.844 kb on - strand at 37.865 kb on + strand, within GFF2804 at 37.865 kb on + strand, within GFF2804 at 37.865 kb on + strand, within GFF2804 at 37.865 kb on + strand, within GFF2804 at 37.865 kb on + strand, within GFF2804 at 37.865 kb on + strand, within GFF2804 at 37.865 kb on + strand, within GFF2804 at 37.865 kb on + strand, within GFF2804 at 37.865 kb on + strand, within GFF2804 at 37.865 kb on + strand, within GFF2804 at 37.866 kb on - strand, within GFF2804 at 37.866 kb on - strand, within GFF2804 at 37.866 kb on - strand, within GFF2804 at 37.866 kb on - strand, within GFF2804 at 37.866 kb on - strand, within GFF2804 at 37.866 kb on - strand, within GFF2804 at 37.866 kb on - strand, within GFF2804 at 37.866 kb on - strand, within GFF2804 at 37.866 kb on - strand, within GFF2804 at 37.985 kb on - strand, within GFF2804 at 37.998 kb on + strand, within GFF2804 at 37.999 kb on - strand, within GFF2804 at 38.039 kb on + strand, within GFF2804 at 38.040 kb on - strand, within GFF2804 at 38.075 kb on + strand, within GFF2804 at 38.076 kb on - strand, within GFF2804 at 38.076 kb on - strand, within GFF2804 at 38.076 kb on - strand, within GFF2804 at 38.162 kb on + strand, within GFF2804 at 38.163 kb on - strand, within GFF2804 at 38.280 kb on + strand, within GFF2804 at 38.280 kb on + strand, within GFF2804 at 38.280 kb on + strand, within GFF2804 at 38.280 kb on + strand, within GFF2804 at 38.281 kb on - strand, within GFF2804 at 38.281 kb on - strand, within GFF2804 at 38.281 kb on - strand, within GFF2804 at 38.281 kb on - strand, within GFF2804 at 38.281 kb on - strand, within GFF2804 at 38.302 kb on + strand, within GFF2804 at 38.321 kb on - strand, within GFF2804 at 38.325 kb on + strand, within GFF2804 at 38.326 kb on - strand, within GFF2804 at 38.388 kb on + strand, within GFF2804 at 38.388 kb on + strand, within GFF2804 at 38.389 kb on - strand, within GFF2804 at 38.389 kb on - strand, within GFF2804 at 38.445 kb on - strand, within GFF2804 at 38.445 kb on - strand, within GFF2804 at 38.511 kb on + strand, within GFF2804 at 38.511 kb on + strand, within GFF2804 at 38.511 kb on + strand, within GFF2804 at 38.511 kb on + strand, within GFF2804 at 38.511 kb on + strand, within GFF2804 at 38.512 kb on - strand, within GFF2804 at 38.532 kb on + strand, within GFF2804 at 38.533 kb on - strand, within GFF2804 at 38.661 kb on - strand at 38.681 kb on + strand at 38.681 kb on + strand at 38.681 kb on + strand at 38.682 kb on - strand at 38.682 kb on - strand at 38.682 kb on - strand at 38.692 kb on - strand at 38.692 kb on - strand at 38.795 kb on - strand, within GFF2805 at 38.795 kb on - strand, within GFF2805 at 38.796 kb on + strand, within GFF2805 at 38.796 kb on + strand, within GFF2805 at 38.796 kb on + strand, within GFF2805 at 38.796 kb on + strand, within GFF2805 at 38.797 kb on - strand, within GFF2805 at 38.797 kb on - strand, within GFF2805 at 38.797 kb on - strand, within GFF2805 at 38.797 kb on - strand, within GFF2805 at 38.797 kb on - strand, within GFF2805 at 38.797 kb on - strand, within GFF2805 at 38.797 kb on - strand, within GFF2805 at 38.797 kb on - strand, within GFF2805 at 38.911 kb on + strand, within GFF2805 at 38.911 kb on + strand, within GFF2805 at 38.911 kb on + strand, within GFF2805 at 38.912 kb on - strand, within GFF2805 at 38.940 kb on + strand, within GFF2805 at 38.940 kb on + strand, within GFF2805 at 38.940 kb on + strand, within GFF2805 at 38.940 kb on + strand, within GFF2805 at 38.940 kb on + strand, within GFF2805 at 38.940 kb on + strand, within GFF2805 at 38.940 kb on + strand, within GFF2805 at 38.940 kb on + strand, within GFF2805 at 38.940 kb on + strand, within GFF2805 at 38.941 kb on - strand, within GFF2805 at 38.941 kb on - strand, within GFF2805 at 38.941 kb on - strand, within GFF2805 at 38.941 kb on - strand, within GFF2805 at 38.941 kb on - strand, within GFF2805 at 38.941 kb on - strand, within GFF2805 at 38.941 kb on - strand, within GFF2805 at 38.941 kb on - strand, within GFF2805 at 38.941 kb on - strand, within GFF2805 at 38.941 kb on - strand, within GFF2805 at 38.941 kb on - strand, within GFF2805 at 38.941 kb on - strand, within GFF2805 at 38.960 kb on - strand, within GFF2805 at 38.960 kb on - strand, within GFF2805 at 38.990 kb on - strand, within GFF2805 at 39.021 kb on + strand, within GFF2805 at 39.022 kb on - strand, within GFF2805 at 39.022 kb on - strand, within GFF2805 at 39.102 kb on + strand, within GFF2805 at 39.103 kb on - strand, within GFF2805 at 39.122 kb on + strand, within GFF2805 at 39.123 kb on - strand, within GFF2805 at 39.123 kb on - strand, within GFF2805 at 39.125 kb on + strand, within GFF2805 at 39.125 kb on + strand, within GFF2805 at 39.125 kb on + strand, within GFF2805 at 39.148 kb on + strand, within GFF2805 at 39.280 kb on + strand, within GFF2805 at 39.281 kb on - strand, within GFF2805 at 39.307 kb on + strand, within GFF2805 at 39.307 kb on + strand, within GFF2805 at 39.307 kb on + strand, within GFF2805 at 39.307 kb on + strand, within GFF2805 at 39.308 kb on - strand, within GFF2805 at 39.308 kb on - strand, within GFF2805 at 39.308 kb on - strand, within GFF2805 at 39.464 kb on + strand at 39.464 kb on + strand at 39.464 kb on + strand at 39.464 kb on + strand at 39.464 kb on + strand at 39.464 kb on + strand at 39.465 kb on - strand at 39.465 kb on - strand at 39.465 kb on - strand at 39.465 kb on - strand at 39.465 kb on - strand at 39.465 kb on - strand at 39.469 kb on + strand at 39.469 kb on + strand at 39.469 kb on + strand at 39.495 kb on + strand at 39.496 kb on - strand at 39.561 kb on + strand at 39.561 kb on + strand at 39.562 kb on - strand at 39.611 kb on + strand, within GFF2806 at 39.612 kb on - strand, within GFF2806 at 39.612 kb on - strand, within GFF2806 at 39.612 kb on - strand, within GFF2806 at 39.612 kb on - strand, within GFF2806 at 39.612 kb on - strand, within GFF2806 at 39.658 kb on + strand, within GFF2806 at 39.658 kb on + strand, within GFF2806 at 39.659 kb on - strand, within GFF2806 at 39.659 kb on - strand, within GFF2806 at 39.816 kb on - strand, within GFF2806 at 39.816 kb on - strand, within GFF2806 at 39.816 kb on - strand, within GFF2806 at 39.816 kb on - strand, within GFF2806 at 39.816 kb on - strand, within GFF2806 at 39.826 kb on - strand, within GFF2806 at 39.831 kb on + strand, within GFF2806 at 39.831 kb on + strand, within GFF2806 at 39.831 kb on + strand, within GFF2806 at 39.831 kb on + strand, within GFF2806 at 39.832 kb on - strand, within GFF2806 at 39.832 kb on - strand, within GFF2806 at 39.922 kb on + strand, within GFF2806 at 39.964 kb on + strand, within GFF2806 at 39.965 kb on - strand, within GFF2806 at 39.965 kb on - strand, within GFF2806 at 39.965 kb on - strand, within GFF2806 at 39.966 kb on + strand, within GFF2806 at 39.966 kb on + strand, within GFF2806 at 39.966 kb on + strand, within GFF2806 at 39.967 kb on - strand, within GFF2806 at 39.967 kb on - strand, within GFF2806 at 39.996 kb on + strand, within GFF2806 at 39.996 kb on + strand, within GFF2806 at 40.084 kb on - strand, within GFF2806 at 40.173 kb on - strand at 40.208 kb on + strand at 40.313 kb on + strand at 40.313 kb on + strand at 40.313 kb on + strand at 40.314 kb on - strand at 40.314 kb on - strand at 40.314 kb on - strand at 40.314 kb on - strand at 40.314 kb on - strand at 40.314 kb on - strand at 40.314 kb on - strand at 40.315 kb on + strand at 40.315 kb on + strand at 40.315 kb on + strand at 40.316 kb on - strand at 40.316 kb on - strand at 40.316 kb on - strand at 40.316 kb on - strand at 40.316 kb on - strand at 40.338 kb on - strand at 40.425 kb on + strand, within GFF2807 at 40.425 kb on + strand, within GFF2807 at 40.427 kb on + strand, within GFF2807 at 40.428 kb on - strand, within GFF2807 at 40.428 kb on - strand, within GFF2807 at 40.459 kb on + strand, within GFF2807
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 1x remove 37,698 + -1.3 37,698 + +0.4 37,699 - -1.0 37,699 - +0.6 37,699 - -0.5 37,721 + +0.7 37,722 - -0.4 37,722 - -1.4 37,745 + -0.3 37,746 - -0.5 37,758 + -2.3 37,759 - -1.3 37,791 + -1.5 37,791 + -0.0 37,791 + -0.5 37,792 - -1.0 37,792 - -0.9 37,792 - +0.0 37,793 + +0.0 37,793 + -1.5 37,793 + -0.7 37,794 - -1.4 37,794 - +0.6 37,797 + -1.0 37,797 + -2.1 37,797 + -0.2 37,797 + +0.0 37,798 - -0.7 37,798 - +0.0 37,798 - -0.7 37,798 - -3.0 37,804 - -0.6 37,813 + +0.2 37,813 + -0.3 37,813 + -0.4 37,814 - -0.4 37,843 + -0.3 37,843 + -0.8 37,844 - -2.9 37,865 + GFF2804 0.12 +0.3 37,865 + GFF2804 0.12 +1.8 37,865 + GFF2804 0.12 +0.5 37,865 + GFF2804 0.12 +0.1 37,865 + GFF2804 0.12 -0.3 37,865 + GFF2804 0.12 +0.0 37,865 + GFF2804 0.12 +0.6 37,865 + GFF2804 0.12 -1.2 37,865 + GFF2804 0.12 -0.8 37,865 + GFF2804 0.12 -0.4 37,866 - GFF2804 0.12 +0.0 37,866 - GFF2804 0.12 +0.0 37,866 - GFF2804 0.12 -1.3 37,866 - GFF2804 0.12 -0.0 37,866 - GFF2804 0.12 -2.3 37,866 - GFF2804 0.12 -0.7 37,866 - GFF2804 0.12 -2.4 37,866 - GFF2804 0.12 +0.6 37,866 - GFF2804 0.12 +0.5 37,985 - GFF2804 0.25 +0.7 37,998 + GFF2804 0.26 +0.4 37,999 - GFF2804 0.26 -1.0 38,039 + GFF2804 0.31 -1.0 38,040 - GFF2804 0.31 -0.7 38,075 + GFF2804 0.35 +0.2 38,076 - GFF2804 0.35 -0.4 38,076 - GFF2804 0.35 -0.5 38,076 - GFF2804 0.35 +0.4 38,162 + GFF2804 0.44 -0.3 38,163 - GFF2804 0.44 +0.3 38,280 + GFF2804 0.57 +0.4 38,280 + GFF2804 0.57 -0.8 38,280 + GFF2804 0.57 +0.7 38,280 + GFF2804 0.57 -1.0 38,281 - GFF2804 0.57 -0.2 38,281 - GFF2804 0.57 +0.0 38,281 - GFF2804 0.57 -0.4 38,281 - GFF2804 0.57 -2.7 38,281 - GFF2804 0.57 +0.6 38,302 + GFF2804 0.59 -0.3 38,321 - GFF2804 0.61 +0.4 38,325 + GFF2804 0.61 -0.4 38,326 - GFF2804 0.62 -1.5 38,388 + GFF2804 0.68 +0.6 38,388 + GFF2804 0.68 -0.3 38,389 - GFF2804 0.68 -1.2 38,389 - GFF2804 0.68 -1.3 38,445 - GFF2804 0.74 -1.1 38,445 - GFF2804 0.74 -0.4 38,511 + GFF2804 0.81 +1.0 38,511 + GFF2804 0.81 -1.5 38,511 + GFF2804 0.81 -0.2 38,511 + GFF2804 0.81 -1.0 38,511 + GFF2804 0.81 -1.0 38,512 - GFF2804 0.82 -0.2 38,532 + GFF2804 0.84 -1.8 38,533 - GFF2804 0.84 -0.3 38,661 - -0.3 38,681 + -1.3 38,681 + -0.1 38,681 + -1.0 38,682 - -0.4 38,682 - -0.1 38,682 - -1.0 38,692 - -1.2 38,692 - -0.1 38,795 - GFF2805 0.13 -1.0 38,795 - GFF2805 0.13 -0.2 38,796 + GFF2805 0.13 +0.5 38,796 + GFF2805 0.13 +0.2 38,796 + GFF2805 0.13 -0.8 38,796 + GFF2805 0.13 -0.8 38,797 - GFF2805 0.14 -1.5 38,797 - GFF2805 0.14 -0.8 38,797 - GFF2805 0.14 +0.0 38,797 - GFF2805 0.14 -1.7 38,797 - GFF2805 0.14 +0.1 38,797 - GFF2805 0.14 +0.3 38,797 - GFF2805 0.14 -1.0 38,797 - GFF2805 0.14 -1.0 38,911 + GFF2805 0.28 +1.4 38,911 + GFF2805 0.28 +1.3 38,911 + GFF2805 0.28 -0.5 38,912 - GFF2805 0.28 -0.2 38,940 + GFF2805 0.32 -1.0 38,940 + GFF2805 0.32 -0.3 38,940 + GFF2805 0.32 -0.9 38,940 + GFF2805 0.32 +0.9 38,940 + GFF2805 0.32 +0.2 38,940 + GFF2805 0.32 +0.5 38,940 + GFF2805 0.32 -0.9 38,940 + GFF2805 0.32 +0.1 38,940 + GFF2805 0.32 -1.0 38,941 - GFF2805 0.32 +0.6 38,941 - GFF2805 0.32 -0.1 38,941 - GFF2805 0.32 +1.0 38,941 - GFF2805 0.32 +0.1 38,941 - GFF2805 0.32 -0.3 38,941 - GFF2805 0.32 -0.1 38,941 - GFF2805 0.32 +0.1 38,941 - GFF2805 0.32 -0.8 38,941 - GFF2805 0.32 -1.3 38,941 - GFF2805 0.32 -0.2 38,941 - GFF2805 0.32 +0.2 38,941 - GFF2805 0.32 -0.1 38,960 - GFF2805 0.34 -0.1 38,960 - GFF2805 0.34 +0.0 38,990 - GFF2805 0.38 +0.5 39,021 + GFF2805 0.42 -0.7 39,022 - GFF2805 0.42 -0.4 39,022 - GFF2805 0.42 -0.2 39,102 + GFF2805 0.52 +0.7 39,103 - GFF2805 0.52 -1.3 39,122 + GFF2805 0.55 -0.1 39,123 - GFF2805 0.55 +0.7 39,123 - GFF2805 0.55 -1.5 39,125 + GFF2805 0.55 -0.2 39,125 + GFF2805 0.55 +0.5 39,125 + GFF2805 0.55 -1.0 39,148 + GFF2805 0.58 -0.5 39,280 + GFF2805 0.74 -0.5 39,281 - GFF2805 0.75 +0.7 39,307 + GFF2805 0.78 -0.7 39,307 + GFF2805 0.78 -1.0 39,307 + GFF2805 0.78 +0.3 39,307 + GFF2805 0.78 +0.5 39,308 - GFF2805 0.78 -0.3 39,308 - GFF2805 0.78 -0.1 39,308 - GFF2805 0.78 -1.3 39,464 + -0.1 39,464 + -0.2 39,464 + +0.2 39,464 + +1.0 39,464 + -1.0 39,464 + +0.0 39,465 - -0.4 39,465 - -0.8 39,465 - -0.4 39,465 - -0.9 39,465 - -0.4 39,465 - -0.6 39,469 + -0.7 39,469 + -0.6 39,469 + -1.3 39,495 + +2.2 39,496 - -0.8 39,561 + -1.0 39,561 + -1.3 39,562 - -1.1 39,611 + GFF2806 0.17 -1.3 39,612 - GFF2806 0.17 -0.2 39,612 - GFF2806 0.17 -0.7 39,612 - GFF2806 0.17 -0.3 39,612 - GFF2806 0.17 -1.5 39,612 - GFF2806 0.17 -1.0 39,658 + GFF2806 0.23 -0.3 39,658 + GFF2806 0.23 +0.5 39,659 - GFF2806 0.24 -1.0 39,659 - GFF2806 0.24 +0.0 39,816 - GFF2806 0.46 -1.5 39,816 - GFF2806 0.46 +0.5 39,816 - GFF2806 0.46 -0.2 39,816 - GFF2806 0.46 -0.0 39,816 - GFF2806 0.46 -0.3 39,826 - GFF2806 0.47 +0.7 39,831 + GFF2806 0.48 -1.4 39,831 + GFF2806 0.48 +0.3 39,831 + GFF2806 0.48 -2.2 39,831 + GFF2806 0.48 +1.0 39,832 - GFF2806 0.48 -1.3 39,832 - GFF2806 0.48 -2.0 39,922 + GFF2806 0.61 -2.4 39,964 + GFF2806 0.67 +0.2 39,965 - GFF2806 0.67 -1.0 39,965 - GFF2806 0.67 +0.0 39,965 - GFF2806 0.67 +0.0 39,966 + GFF2806 0.68 +0.0 39,966 + GFF2806 0.68 +0.7 39,966 + GFF2806 0.68 -0.5 39,967 - GFF2806 0.68 +0.3 39,967 - GFF2806 0.68 -0.5 39,996 + GFF2806 0.72 -0.5 39,996 + GFF2806 0.72 +0.5 40,084 - GFF2806 0.84 -0.5 40,173 - -1.3 40,208 + +0.7 40,313 + +1.0 40,313 + +0.4 40,313 + -0.4 40,314 - -3.2 40,314 - -1.0 40,314 - -0.6 40,314 - -0.0 40,314 - -0.3 40,314 - -1.0 40,314 - +0.8 40,315 + -0.3 40,315 + -0.9 40,315 + -0.1 40,316 - +0.6 40,316 - +0.2 40,316 - +0.6 40,316 - +0.0 40,316 - -1.0 40,338 - -1.0 40,425 + GFF2807 0.16 +0.3 40,425 + GFF2807 0.16 -0.7 40,427 + GFF2807 0.16 -0.5 40,428 - GFF2807 0.16 +0.2 40,428 - GFF2807 0.16 -0.5 40,459 + GFF2807 0.18 +1.4
Or see this region's nucleotide sequence