Experiment: Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2151 and GFF2152 overlap by 8 nucleotides GFF2152 and GFF2153 are separated by 17 nucleotides GFF2153 and GFF2154 are separated by 192 nucleotides
GFF2151 - hypothetical protein, at 197,601 to 198,620
GFF2151
GFF2152 - Transcriptional regulatory protein YpdB, at 198,613 to 199,359
GFF2152
GFF2153 - hypothetical protein, at 199,377 to 200,048
GFF2153
GFF2154 - Porphobilinogen deaminase, at 200,241 to 201,185
GFF2154
Position (kb) 198
199
200 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 197.644 kb on + strand at 197.644 kb on + strand at 197.644 kb on + strand at 197.644 kb on + strand at 197.645 kb on - strand at 197.645 kb on - strand at 197.645 kb on - strand at 197.645 kb on - strand at 197.645 kb on - strand at 197.645 kb on - strand at 197.650 kb on + strand at 197.650 kb on + strand at 197.650 kb on + strand at 197.650 kb on + strand at 197.650 kb on + strand at 197.650 kb on + strand at 197.650 kb on + strand at 197.650 kb on + strand at 197.650 kb on + strand at 197.650 kb on + strand at 197.650 kb on + strand at 197.650 kb on + strand at 197.650 kb on + strand at 197.650 kb on + strand at 197.651 kb on - strand at 197.651 kb on - strand at 197.651 kb on - strand at 197.651 kb on - strand at 197.656 kb on + strand at 197.656 kb on + strand at 197.657 kb on - strand at 197.657 kb on - strand at 197.657 kb on - strand at 197.657 kb on - strand at 197.697 kb on + strand at 197.697 kb on + strand at 197.697 kb on + strand at 197.720 kb on + strand, within GFF2151 at 197.720 kb on + strand, within GFF2151 at 197.720 kb on + strand, within GFF2151 at 197.721 kb on - strand, within GFF2151 at 197.832 kb on + strand, within GFF2151 at 197.898 kb on + strand, within GFF2151 at 197.899 kb on - strand, within GFF2151 at 197.947 kb on + strand, within GFF2151 at 197.947 kb on + strand, within GFF2151 at 197.947 kb on + strand, within GFF2151 at 197.948 kb on - strand, within GFF2151 at 197.948 kb on - strand, within GFF2151 at 197.982 kb on + strand, within GFF2151 at 197.982 kb on + strand, within GFF2151 at 197.983 kb on - strand, within GFF2151 at 197.983 kb on - strand, within GFF2151 at 197.983 kb on - strand, within GFF2151 at 197.985 kb on + strand, within GFF2151 at 198.077 kb on + strand, within GFF2151 at 198.077 kb on + strand, within GFF2151 at 198.078 kb on - strand, within GFF2151 at 198.080 kb on + strand, within GFF2151 at 198.081 kb on - strand, within GFF2151 at 198.081 kb on - strand, within GFF2151 at 198.157 kb on + strand, within GFF2151 at 198.228 kb on + strand, within GFF2151 at 198.229 kb on - strand, within GFF2151 at 198.229 kb on - strand, within GFF2151 at 198.351 kb on + strand, within GFF2151 at 198.352 kb on - strand, within GFF2151 at 198.352 kb on - strand, within GFF2151 at 198.392 kb on + strand, within GFF2151 at 198.392 kb on + strand, within GFF2151 at 198.393 kb on - strand, within GFF2151 at 198.393 kb on - strand, within GFF2151 at 198.393 kb on - strand, within GFF2151 at 198.433 kb on + strand, within GFF2151 at 198.476 kb on + strand, within GFF2151 at 198.477 kb on - strand, within GFF2151 at 198.494 kb on + strand, within GFF2151 at 198.495 kb on - strand, within GFF2151 at 198.495 kb on - strand, within GFF2151 at 198.519 kb on + strand at 198.564 kb on + strand at 198.564 kb on + strand at 198.564 kb on + strand at 198.564 kb on + strand at 198.564 kb on + strand at 198.564 kb on + strand at 198.564 kb on + strand at 198.565 kb on - strand at 198.565 kb on - strand at 198.566 kb on + strand at 198.567 kb on - strand at 198.697 kb on + strand, within GFF2152 at 198.868 kb on + strand, within GFF2152 at 198.868 kb on + strand, within GFF2152 at 198.868 kb on + strand, within GFF2152 at 198.869 kb on - strand, within GFF2152 at 198.869 kb on - strand, within GFF2152 at 198.869 kb on - strand, within GFF2152 at 198.869 kb on - strand, within GFF2152 at 198.878 kb on - strand, within GFF2152 at 198.913 kb on + strand, within GFF2152 at 198.913 kb on + strand, within GFF2152 at 198.913 kb on + strand, within GFF2152 at 198.913 kb on + strand, within GFF2152 at 198.913 kb on + strand, within GFF2152 at 198.913 kb on + strand, within GFF2152 at 198.913 kb on + strand, within GFF2152 at 198.914 kb on - strand, within GFF2152 at 198.914 kb on - strand, within GFF2152 at 199.028 kb on + strand, within GFF2152 at 199.028 kb on + strand, within GFF2152 at 199.028 kb on + strand, within GFF2152 at 199.028 kb on + strand, within GFF2152 at 199.028 kb on + strand, within GFF2152 at 199.029 kb on - strand, within GFF2152 at 199.029 kb on - strand, within GFF2152 at 199.029 kb on - strand, within GFF2152 at 199.029 kb on - strand, within GFF2152 at 199.081 kb on + strand, within GFF2152 at 199.081 kb on + strand, within GFF2152 at 199.081 kb on + strand, within GFF2152 at 199.082 kb on - strand, within GFF2152 at 199.082 kb on - strand, within GFF2152 at 199.082 kb on - strand, within GFF2152 at 199.082 kb on - strand, within GFF2152 at 199.102 kb on + strand, within GFF2152 at 199.102 kb on + strand, within GFF2152 at 199.102 kb on + strand, within GFF2152 at 199.102 kb on + strand, within GFF2152 at 199.102 kb on + strand, within GFF2152 at 199.102 kb on + strand, within GFF2152 at 199.103 kb on - strand, within GFF2152 at 199.103 kb on - strand, within GFF2152 at 199.103 kb on - strand, within GFF2152 at 199.103 kb on - strand, within GFF2152 at 199.103 kb on - strand, within GFF2152 at 199.103 kb on - strand, within GFF2152 at 199.103 kb on - strand, within GFF2152 at 199.108 kb on - strand, within GFF2152 at 199.133 kb on - strand, within GFF2152 at 199.136 kb on + strand, within GFF2152 at 199.136 kb on + strand, within GFF2152 at 199.137 kb on - strand, within GFF2152 at 199.137 kb on - strand, within GFF2152 at 199.192 kb on - strand, within GFF2152 at 199.192 kb on - strand, within GFF2152 at 199.192 kb on - strand, within GFF2152 at 199.193 kb on + strand, within GFF2152 at 199.193 kb on + strand, within GFF2152 at 199.193 kb on + strand, within GFF2152 at 199.194 kb on - strand, within GFF2152 at 199.194 kb on - strand, within GFF2152 at 199.194 kb on - strand, within GFF2152 at 199.194 kb on - strand, within GFF2152 at 199.194 kb on - strand, within GFF2152 at 199.296 kb on + strand at 199.296 kb on + strand at 199.296 kb on + strand at 199.297 kb on - strand at 199.297 kb on - strand at 199.303 kb on - strand at 199.347 kb on + strand at 199.347 kb on + strand at 199.348 kb on - strand at 199.348 kb on - strand at 199.348 kb on - strand at 199.357 kb on + strand at 199.357 kb on + strand at 199.357 kb on + strand at 199.357 kb on + strand at 199.357 kb on + strand at 199.357 kb on + strand at 199.357 kb on + strand at 199.357 kb on + strand at 199.357 kb on + strand at 199.357 kb on + strand at 199.358 kb on - strand at 199.358 kb on - strand at 199.358 kb on - strand at 199.358 kb on - strand at 199.358 kb on - strand at 199.358 kb on - strand at 199.358 kb on - strand at 199.358 kb on - strand at 199.358 kb on - strand at 199.358 kb on - strand at 199.358 kb on - strand at 199.358 kb on - strand at 199.358 kb on - strand at 199.358 kb on - strand at 199.361 kb on + strand at 199.361 kb on + strand at 199.361 kb on + strand at 199.361 kb on + strand at 199.361 kb on + strand at 199.370 kb on + strand at 199.370 kb on + strand at 199.370 kb on + strand at 199.404 kb on + strand at 199.404 kb on + strand at 199.404 kb on + strand at 199.405 kb on - strand at 199.405 kb on - strand at 199.459 kb on - strand, within GFF2153 at 199.488 kb on + strand, within GFF2153 at 199.488 kb on + strand, within GFF2153 at 199.488 kb on + strand, within GFF2153 at 199.489 kb on - strand, within GFF2153 at 199.489 kb on - strand, within GFF2153 at 199.489 kb on - strand, within GFF2153 at 199.497 kb on + strand, within GFF2153 at 199.497 kb on + strand, within GFF2153 at 199.498 kb on - strand, within GFF2153 at 199.498 kb on - strand, within GFF2153 at 199.498 kb on - strand, within GFF2153 at 199.518 kb on + strand, within GFF2153 at 199.518 kb on + strand, within GFF2153 at 199.518 kb on + strand, within GFF2153 at 199.519 kb on - strand, within GFF2153 at 199.519 kb on - strand, within GFF2153 at 199.561 kb on + strand, within GFF2153 at 199.561 kb on + strand, within GFF2153 at 199.561 kb on + strand, within GFF2153 at 199.562 kb on - strand, within GFF2153 at 199.562 kb on - strand, within GFF2153 at 199.581 kb on + strand, within GFF2153 at 199.581 kb on + strand, within GFF2153 at 199.637 kb on + strand, within GFF2153 at 199.638 kb on - strand, within GFF2153 at 199.650 kb on + strand, within GFF2153 at 199.650 kb on + strand, within GFF2153 at 199.651 kb on - strand, within GFF2153 at 199.651 kb on - strand, within GFF2153 at 199.651 kb on - strand, within GFF2153 at 199.651 kb on - strand, within GFF2153 at 199.699 kb on + strand, within GFF2153 at 199.699 kb on + strand, within GFF2153 at 199.700 kb on - strand, within GFF2153 at 199.700 kb on - strand, within GFF2153 at 199.716 kb on + strand, within GFF2153 at 199.717 kb on - strand, within GFF2153 at 199.717 kb on - strand, within GFF2153 at 199.789 kb on + strand, within GFF2153 at 199.789 kb on + strand, within GFF2153 at 199.789 kb on + strand, within GFF2153 at 199.790 kb on - strand, within GFF2153 at 199.790 kb on - strand, within GFF2153 at 199.790 kb on - strand, within GFF2153 at 199.794 kb on + strand, within GFF2153 at 199.794 kb on + strand, within GFF2153 at 199.794 kb on + strand, within GFF2153 at 199.794 kb on + strand, within GFF2153 at 199.795 kb on - strand, within GFF2153 at 199.795 kb on - strand, within GFF2153 at 199.795 kb on - strand, within GFF2153 at 199.805 kb on + strand, within GFF2153 at 199.806 kb on - strand, within GFF2153 at 199.806 kb on - strand, within GFF2153 at 199.806 kb on - strand, within GFF2153 at 199.829 kb on - strand, within GFF2153 at 199.829 kb on - strand, within GFF2153 at 199.829 kb on - strand, within GFF2153 at 199.829 kb on - strand, within GFF2153 at 199.829 kb on - strand, within GFF2153 at 199.844 kb on + strand, within GFF2153 at 199.844 kb on + strand, within GFF2153 at 199.845 kb on - strand, within GFF2153 at 199.845 kb on - strand, within GFF2153 at 199.902 kb on - strand, within GFF2153 at 199.972 kb on - strand, within GFF2153 at 199.990 kb on - strand at 200.041 kb on + strand at 200.042 kb on - strand at 200.042 kb on - strand at 200.052 kb on + strand at 200.052 kb on + strand at 200.052 kb on + strand at 200.053 kb on - strand at 200.053 kb on - strand at 200.053 kb on - strand at 200.053 kb on - strand at 200.065 kb on + strand at 200.066 kb on - strand at 200.071 kb on + strand at 200.071 kb on + strand at 200.121 kb on + strand at 200.122 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 1x remove 197,644 + -2.0 197,644 + -0.1 197,644 + -1.5 197,644 + -0.1 197,645 - -0.3 197,645 - -0.3 197,645 - -0.4 197,645 - -0.4 197,645 - -1.5 197,645 - +0.0 197,650 + -0.3 197,650 + -0.1 197,650 + +0.1 197,650 + +0.8 197,650 + -0.7 197,650 + -1.3 197,650 + +1.4 197,650 + -0.0 197,650 + +0.3 197,650 + -0.5 197,650 + +0.6 197,650 + +0.8 197,650 + +0.1 197,650 + +0.1 197,651 - -1.1 197,651 - -0.1 197,651 - -1.2 197,651 - +0.8 197,656 + -0.5 197,656 + +0.2 197,657 - -0.5 197,657 - -1.3 197,657 - -0.7 197,657 - -0.7 197,697 + +0.4 197,697 + -1.5 197,697 + -1.6 197,720 + GFF2151 0.12 -1.3 197,720 + GFF2151 0.12 +0.3 197,720 + GFF2151 0.12 -0.2 197,721 - GFF2151 0.12 -0.5 197,832 + GFF2151 0.23 +0.8 197,898 + GFF2151 0.29 -0.2 197,899 - GFF2151 0.29 -0.1 197,947 + GFF2151 0.34 +0.4 197,947 + GFF2151 0.34 -0.4 197,947 + GFF2151 0.34 +0.5 197,948 - GFF2151 0.34 -1.2 197,948 - GFF2151 0.34 -0.9 197,982 + GFF2151 0.37 +0.2 197,982 + GFF2151 0.37 -0.7 197,983 - GFF2151 0.37 -0.2 197,983 - GFF2151 0.37 +1.1 197,983 - GFF2151 0.37 -0.1 197,985 + GFF2151 0.38 -1.6 198,077 + GFF2151 0.47 -0.3 198,077 + GFF2151 0.47 -0.7 198,078 - GFF2151 0.47 -1.6 198,080 + GFF2151 0.47 -0.4 198,081 - GFF2151 0.47 -0.9 198,081 - GFF2151 0.47 -1.3 198,157 + GFF2151 0.55 -0.6 198,228 + GFF2151 0.61 -1.8 198,229 - GFF2151 0.62 +0.1 198,229 - GFF2151 0.62 +1.3 198,351 + GFF2151 0.74 +0.1 198,352 - GFF2151 0.74 -0.6 198,352 - GFF2151 0.74 +0.1 198,392 + GFF2151 0.78 -0.9 198,392 + GFF2151 0.78 +0.1 198,393 - GFF2151 0.78 -0.2 198,393 - GFF2151 0.78 -0.5 198,393 - GFF2151 0.78 -1.6 198,433 + GFF2151 0.82 -0.2 198,476 + GFF2151 0.86 -2.3 198,477 - GFF2151 0.86 +0.1 198,494 + GFF2151 0.88 -0.2 198,495 - GFF2151 0.88 +0.2 198,495 - GFF2151 0.88 -0.6 198,519 + -0.9 198,564 + +0.6 198,564 + -1.2 198,564 + +0.6 198,564 + -0.4 198,564 + -0.1 198,564 + -0.0 198,564 + +1.2 198,565 - -0.4 198,565 - -0.9 198,566 + -0.6 198,567 - +0.2 198,697 + GFF2152 0.11 -0.4 198,868 + GFF2152 0.34 -2.1 198,868 + GFF2152 0.34 +0.2 198,868 + GFF2152 0.34 +1.3 198,869 - GFF2152 0.34 -0.0 198,869 - GFF2152 0.34 +0.1 198,869 - GFF2152 0.34 +0.9 198,869 - GFF2152 0.34 +0.6 198,878 - GFF2152 0.35 -0.6 198,913 + GFF2152 0.40 -1.3 198,913 + GFF2152 0.40 -0.8 198,913 + GFF2152 0.40 -0.8 198,913 + GFF2152 0.40 -0.2 198,913 + GFF2152 0.40 -1.2 198,913 + GFF2152 0.40 -0.1 198,913 + GFF2152 0.40 +0.1 198,914 - GFF2152 0.40 +0.7 198,914 - GFF2152 0.40 -0.9 199,028 + GFF2152 0.56 -1.0 199,028 + GFF2152 0.56 +0.5 199,028 + GFF2152 0.56 -0.4 199,028 + GFF2152 0.56 -1.5 199,028 + GFF2152 0.56 +0.1 199,029 - GFF2152 0.56 -1.4 199,029 - GFF2152 0.56 -0.2 199,029 - GFF2152 0.56 +0.3 199,029 - GFF2152 0.56 -1.5 199,081 + GFF2152 0.63 +0.7 199,081 + GFF2152 0.63 -0.9 199,081 + GFF2152 0.63 -0.6 199,082 - GFF2152 0.63 -0.9 199,082 - GFF2152 0.63 -0.0 199,082 - GFF2152 0.63 -0.9 199,082 - GFF2152 0.63 -0.5 199,102 + GFF2152 0.65 -0.7 199,102 + GFF2152 0.65 -1.0 199,102 + GFF2152 0.65 -0.3 199,102 + GFF2152 0.65 +0.2 199,102 + GFF2152 0.65 -2.9 199,102 + GFF2152 0.65 -0.1 199,103 - GFF2152 0.66 -1.0 199,103 - GFF2152 0.66 -1.4 199,103 - GFF2152 0.66 -0.3 199,103 - GFF2152 0.66 -0.2 199,103 - GFF2152 0.66 +0.3 199,103 - GFF2152 0.66 +1.4 199,103 - GFF2152 0.66 +1.1 199,108 - GFF2152 0.66 -0.5 199,133 - GFF2152 0.70 -1.0 199,136 + GFF2152 0.70 -0.1 199,136 + GFF2152 0.70 -0.9 199,137 - GFF2152 0.70 -0.7 199,137 - GFF2152 0.70 -1.1 199,192 - GFF2152 0.78 -0.4 199,192 - GFF2152 0.78 +0.5 199,192 - GFF2152 0.78 +1.1 199,193 + GFF2152 0.78 -1.2 199,193 + GFF2152 0.78 -0.6 199,193 + GFF2152 0.78 +1.1 199,194 - GFF2152 0.78 -0.8 199,194 - GFF2152 0.78 -1.3 199,194 - GFF2152 0.78 -0.7 199,194 - GFF2152 0.78 +0.1 199,194 - GFF2152 0.78 -0.5 199,296 + -0.5 199,296 + -0.6 199,296 + -1.4 199,297 - -0.8 199,297 - +0.1 199,303 - +0.4 199,347 + -0.6 199,347 + +1.3 199,348 - -2.3 199,348 - -1.3 199,348 - -1.3 199,357 + +0.3 199,357 + +2.4 199,357 + -1.3 199,357 + -1.8 199,357 + -0.9 199,357 + -0.8 199,357 + -0.9 199,357 + -0.3 199,357 + -0.8 199,357 + -0.8 199,358 - -0.8 199,358 - +0.2 199,358 - -0.4 199,358 - -0.2 199,358 - +0.1 199,358 - +0.9 199,358 - -0.9 199,358 - -1.3 199,358 - -1.9 199,358 - -0.5 199,358 - -1.9 199,358 - +1.2 199,358 - -0.8 199,358 - -0.6 199,361 + -0.1 199,361 + +0.2 199,361 + -0.2 199,361 + -1.4 199,361 + -1.6 199,370 + -0.3 199,370 + -0.4 199,370 + -0.5 199,404 + -0.9 199,404 + -0.8 199,404 + -0.5 199,405 - -0.5 199,405 - -0.5 199,459 - GFF2153 0.12 -0.7 199,488 + GFF2153 0.17 -0.6 199,488 + GFF2153 0.17 -0.4 199,488 + GFF2153 0.17 +0.3 199,489 - GFF2153 0.17 -0.4 199,489 - GFF2153 0.17 +0.6 199,489 - GFF2153 0.17 +0.6 199,497 + GFF2153 0.18 -0.2 199,497 + GFF2153 0.18 +1.1 199,498 - GFF2153 0.18 -1.6 199,498 - GFF2153 0.18 -0.9 199,498 - GFF2153 0.18 -0.7 199,518 + GFF2153 0.21 +0.4 199,518 + GFF2153 0.21 -1.3 199,518 + GFF2153 0.21 -0.3 199,519 - GFF2153 0.21 -1.7 199,519 - GFF2153 0.21 +0.3 199,561 + GFF2153 0.27 +0.2 199,561 + GFF2153 0.27 -0.4 199,561 + GFF2153 0.27 +0.1 199,562 - GFF2153 0.28 +1.1 199,562 - GFF2153 0.28 +0.4 199,581 + GFF2153 0.30 -0.6 199,581 + GFF2153 0.30 -0.8 199,637 + GFF2153 0.39 +0.4 199,638 - GFF2153 0.39 -0.1 199,650 + GFF2153 0.41 -0.7 199,650 + GFF2153 0.41 -1.5 199,651 - GFF2153 0.41 -1.9 199,651 - GFF2153 0.41 -0.9 199,651 - GFF2153 0.41 -1.5 199,651 - GFF2153 0.41 -0.1 199,699 + GFF2153 0.48 -0.2 199,699 + GFF2153 0.48 -1.8 199,700 - GFF2153 0.48 -1.8 199,700 - GFF2153 0.48 +0.3 199,716 + GFF2153 0.50 -1.8 199,717 - GFF2153 0.51 -1.3 199,717 - GFF2153 0.51 -0.5 199,789 + GFF2153 0.61 -0.2 199,789 + GFF2153 0.61 +0.1 199,789 + GFF2153 0.61 +0.6 199,790 - GFF2153 0.61 -1.3 199,790 - GFF2153 0.61 -0.6 199,790 - GFF2153 0.61 -1.5 199,794 + GFF2153 0.62 -0.2 199,794 + GFF2153 0.62 -0.2 199,794 + GFF2153 0.62 -0.5 199,794 + GFF2153 0.62 -1.1 199,795 - GFF2153 0.62 -0.5 199,795 - GFF2153 0.62 -0.8 199,795 - GFF2153 0.62 -0.9 199,805 + GFF2153 0.64 -1.1 199,806 - GFF2153 0.64 -2.1 199,806 - GFF2153 0.64 -1.1 199,806 - GFF2153 0.64 +1.2 199,829 - GFF2153 0.67 -1.5 199,829 - GFF2153 0.67 -0.5 199,829 - GFF2153 0.67 -1.4 199,829 - GFF2153 0.67 -1.8 199,829 - GFF2153 0.67 -0.9 199,844 + GFF2153 0.69 -0.4 199,844 + GFF2153 0.69 -1.0 199,845 - GFF2153 0.70 -0.7 199,845 - GFF2153 0.70 -1.0 199,902 - GFF2153 0.78 -0.4 199,972 - GFF2153 0.89 -0.3 199,990 - -0.9 200,041 + -1.3 200,042 - -0.3 200,042 - -0.3 200,052 + +0.1 200,052 + -1.6 200,052 + -0.3 200,053 - -0.5 200,053 - -0.9 200,053 - +0.5 200,053 - -0.6 200,065 + +0.1 200,066 - -0.9 200,071 + -2.1 200,071 + -0.6 200,121 + -0.4 200,122 - +0.1
Or see this region's nucleotide sequence