Experiment: Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF211 and GFF212 are separated by 5 nucleotides GFF212 and GFF213 are separated by 62 nucleotides GFF213 and GFF214 are separated by 348 nucleotides
GFF211 - Serine/threonine-protein kinase PknD, at 216,445 to 218,235
GFF211
GFF212 - Cyanate hydratase, at 218,241 to 218,684
GFF212
GFF213 - putative formate transporter 1, at 218,747 to 219,556
GFF213
GFF214 - Nitrate regulatory protein, at 219,905 to 221,182
GFF214
Position (kb) 218
219
220 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 217.760 kb on + strand, within GFF211 at 217.760 kb on + strand, within GFF211 at 217.760 kb on + strand, within GFF211 at 217.761 kb on - strand, within GFF211 at 217.761 kb on - strand, within GFF211 at 217.761 kb on - strand, within GFF211 at 217.764 kb on + strand, within GFF211 at 217.764 kb on + strand, within GFF211 at 217.764 kb on + strand, within GFF211 at 217.764 kb on + strand, within GFF211 at 217.764 kb on + strand, within GFF211 at 217.764 kb on + strand, within GFF211 at 217.764 kb on + strand, within GFF211 at 217.764 kb on + strand, within GFF211 at 217.765 kb on - strand, within GFF211 at 217.765 kb on - strand, within GFF211 at 217.765 kb on - strand, within GFF211 at 217.765 kb on - strand, within GFF211 at 217.765 kb on - strand, within GFF211 at 217.765 kb on - strand, within GFF211 at 217.765 kb on - strand, within GFF211 at 217.765 kb on - strand, within GFF211 at 217.765 kb on - strand, within GFF211 at 217.765 kb on - strand, within GFF211 at 217.765 kb on - strand, within GFF211 at 217.765 kb on - strand, within GFF211 at 217.765 kb on - strand, within GFF211 at 217.858 kb on + strand, within GFF211 at 217.858 kb on + strand, within GFF211 at 217.859 kb on - strand, within GFF211 at 217.878 kb on + strand, within GFF211 at 217.878 kb on + strand, within GFF211 at 217.900 kb on - strand, within GFF211 at 217.907 kb on + strand, within GFF211 at 217.907 kb on + strand, within GFF211 at 217.907 kb on + strand, within GFF211 at 217.907 kb on + strand, within GFF211 at 217.907 kb on + strand, within GFF211 at 217.907 kb on + strand, within GFF211 at 217.969 kb on - strand, within GFF211 at 217.972 kb on - strand, within GFF211 at 217.972 kb on - strand, within GFF211 at 218.015 kb on - strand, within GFF211 at 218.015 kb on - strand, within GFF211 at 218.030 kb on + strand, within GFF211 at 218.039 kb on - strand, within GFF211 at 218.040 kb on + strand, within GFF211 at 218.040 kb on + strand, within GFF211 at 218.061 kb on + strand at 218.061 kb on + strand at 218.062 kb on - strand at 218.064 kb on + strand at 218.064 kb on + strand at 218.065 kb on - strand at 218.065 kb on - strand at 218.089 kb on - strand at 218.113 kb on + strand at 218.113 kb on + strand at 218.114 kb on - strand at 218.114 kb on - strand at 218.114 kb on - strand at 218.114 kb on - strand at 218.216 kb on - strand at 218.242 kb on + strand at 218.242 kb on + strand at 218.246 kb on - strand at 218.255 kb on - strand at 218.328 kb on - strand, within GFF212 at 218.330 kb on + strand, within GFF212 at 218.330 kb on + strand, within GFF212 at 218.330 kb on + strand, within GFF212 at 218.331 kb on - strand, within GFF212 at 218.331 kb on - strand, within GFF212 at 218.331 kb on - strand, within GFF212 at 218.384 kb on + strand, within GFF212 at 218.385 kb on - strand, within GFF212 at 218.416 kb on + strand, within GFF212 at 218.469 kb on - strand, within GFF212 at 218.469 kb on - strand, within GFF212 at 218.470 kb on + strand, within GFF212 at 218.470 kb on + strand, within GFF212 at 218.471 kb on - strand, within GFF212 at 218.471 kb on - strand, within GFF212 at 218.471 kb on - strand, within GFF212 at 218.476 kb on - strand, within GFF212 at 218.484 kb on + strand, within GFF212 at 218.484 kb on + strand, within GFF212 at 218.484 kb on + strand, within GFF212 at 218.485 kb on - strand, within GFF212 at 218.485 kb on - strand, within GFF212 at 218.493 kb on + strand, within GFF212 at 218.532 kb on - strand, within GFF212 at 218.568 kb on + strand, within GFF212 at 218.568 kb on + strand, within GFF212 at 218.569 kb on - strand, within GFF212 at 218.569 kb on - strand, within GFF212 at 218.688 kb on + strand at 218.688 kb on + strand at 218.688 kb on + strand at 218.689 kb on - strand at 218.748 kb on + strand at 218.749 kb on - strand at 218.755 kb on + strand at 218.755 kb on + strand at 218.755 kb on + strand at 218.755 kb on + strand at 218.756 kb on - strand at 218.756 kb on - strand at 218.785 kb on + strand at 218.787 kb on + strand at 218.787 kb on + strand at 218.787 kb on + strand at 218.788 kb on - strand at 218.788 kb on - strand at 218.863 kb on - strand, within GFF213 at 218.863 kb on - strand, within GFF213 at 218.863 kb on - strand, within GFF213 at 218.917 kb on + strand, within GFF213 at 218.918 kb on - strand, within GFF213 at 218.918 kb on - strand, within GFF213 at 218.918 kb on - strand, within GFF213 at 219.062 kb on - strand, within GFF213 at 219.122 kb on + strand, within GFF213 at 219.163 kb on + strand, within GFF213 at 219.164 kb on - strand, within GFF213 at 219.164 kb on - strand, within GFF213 at 219.172 kb on - strand, within GFF213 at 219.182 kb on - strand, within GFF213 at 219.229 kb on + strand, within GFF213 at 219.233 kb on + strand, within GFF213 at 219.234 kb on - strand, within GFF213 at 219.247 kb on + strand, within GFF213 at 219.412 kb on + strand, within GFF213 at 219.412 kb on + strand, within GFF213 at 219.413 kb on - strand, within GFF213 at 219.413 kb on - strand, within GFF213 at 219.457 kb on + strand, within GFF213 at 219.458 kb on - strand, within GFF213 at 219.459 kb on + strand, within GFF213 at 219.459 kb on + strand, within GFF213 at 219.460 kb on - strand, within GFF213 at 219.490 kb on + strand at 219.515 kb on + strand at 219.523 kb on + strand at 219.524 kb on - strand at 219.545 kb on + strand at 219.546 kb on - strand at 219.549 kb on + strand at 219.549 kb on + strand at 219.550 kb on - strand at 219.550 kb on - strand at 219.550 kb on - strand at 219.561 kb on + strand at 219.561 kb on + strand at 219.561 kb on + strand at 219.561 kb on + strand at 219.568 kb on + strand at 219.568 kb on + strand at 219.627 kb on - strand at 219.676 kb on + strand at 219.677 kb on - strand at 219.718 kb on + strand at 219.718 kb on + strand at 219.718 kb on + strand at 219.718 kb on + strand at 219.718 kb on + strand at 219.719 kb on - strand at 219.719 kb on - strand at 219.719 kb on - strand at 219.719 kb on - strand at 219.719 kb on - strand at 219.719 kb on - strand at 219.719 kb on - strand at 219.719 kb on - strand at 219.719 kb on - strand at 219.723 kb on - strand at 219.764 kb on + strand at 219.764 kb on + strand at 219.765 kb on - strand at 219.775 kb on - strand at 219.783 kb on + strand at 219.830 kb on - strand at 219.843 kb on - strand at 219.844 kb on + strand at 219.850 kb on + strand at 219.850 kb on + strand at 219.850 kb on + strand at 219.850 kb on + strand at 219.851 kb on - strand at 219.851 kb on - strand at 219.851 kb on - strand at 219.851 kb on - strand at 219.854 kb on + strand at 219.856 kb on + strand at 219.857 kb on - strand at 219.857 kb on - strand at 219.857 kb on - strand at 219.865 kb on + strand at 219.865 kb on + strand at 219.865 kb on + strand at 219.866 kb on - strand at 219.866 kb on - strand at 219.866 kb on - strand at 219.867 kb on + strand at 219.867 kb on + strand at 220.018 kb on + strand at 220.035 kb on - strand, within GFF214 at 220.085 kb on - strand, within GFF214 at 220.150 kb on + strand, within GFF214 at 220.150 kb on + strand, within GFF214 at 220.150 kb on + strand, within GFF214 at 220.150 kb on + strand, within GFF214 at 220.150 kb on + strand, within GFF214 at 220.151 kb on - strand, within GFF214 at 220.151 kb on - strand, within GFF214 at 220.151 kb on - strand, within GFF214 at 220.151 kb on - strand, within GFF214 at 220.151 kb on - strand, within GFF214 at 220.169 kb on - strand, within GFF214 at 220.174 kb on + strand, within GFF214 at 220.174 kb on + strand, within GFF214 at 220.175 kb on - strand, within GFF214 at 220.175 kb on - strand, within GFF214 at 220.175 kb on - strand, within GFF214 at 220.234 kb on + strand, within GFF214 at 220.235 kb on - strand, within GFF214 at 220.235 kb on - strand, within GFF214 at 220.235 kb on - strand, within GFF214 at 220.235 kb on - strand, within GFF214 at 220.235 kb on - strand, within GFF214 at 220.237 kb on + strand, within GFF214 at 220.238 kb on - strand, within GFF214 at 220.238 kb on - strand, within GFF214 at 220.284 kb on + strand, within GFF214 at 220.418 kb on - strand, within GFF214 at 220.432 kb on + strand, within GFF214 at 220.496 kb on + strand, within GFF214 at 220.497 kb on - strand, within GFF214 at 220.533 kb on - strand, within GFF214
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 1x remove 217,760 + GFF211 0.73 +0.8 217,760 + GFF211 0.73 -0.5 217,760 + GFF211 0.73 +0.4 217,761 - GFF211 0.73 -0.0 217,761 - GFF211 0.73 -0.2 217,761 - GFF211 0.73 +0.3 217,764 + GFF211 0.74 +1.0 217,764 + GFF211 0.74 -0.4 217,764 + GFF211 0.74 +0.3 217,764 + GFF211 0.74 +0.2 217,764 + GFF211 0.74 -0.2 217,764 + GFF211 0.74 -0.0 217,764 + GFF211 0.74 -0.2 217,764 + GFF211 0.74 +1.3 217,765 - GFF211 0.74 -1.3 217,765 - GFF211 0.74 +0.2 217,765 - GFF211 0.74 -1.2 217,765 - GFF211 0.74 -0.6 217,765 - GFF211 0.74 -1.0 217,765 - GFF211 0.74 -0.4 217,765 - GFF211 0.74 -1.6 217,765 - GFF211 0.74 -0.6 217,765 - GFF211 0.74 +1.5 217,765 - GFF211 0.74 -0.6 217,765 - GFF211 0.74 +1.0 217,765 - GFF211 0.74 +0.5 217,765 - GFF211 0.74 -1.0 217,858 + GFF211 0.79 -1.0 217,858 + GFF211 0.79 -1.7 217,859 - GFF211 0.79 +0.2 217,878 + GFF211 0.80 -0.6 217,878 + GFF211 0.80 -1.0 217,900 - GFF211 0.81 +0.4 217,907 + GFF211 0.82 +0.7 217,907 + GFF211 0.82 -1.3 217,907 + GFF211 0.82 -1.2 217,907 + GFF211 0.82 -0.2 217,907 + GFF211 0.82 -1.3 217,907 + GFF211 0.82 -0.9 217,969 - GFF211 0.85 -2.3 217,972 - GFF211 0.85 +0.6 217,972 - GFF211 0.85 +0.3 218,015 - GFF211 0.88 -1.6 218,015 - GFF211 0.88 +1.2 218,030 + GFF211 0.88 -0.7 218,039 - GFF211 0.89 -0.6 218,040 + GFF211 0.89 -1.8 218,040 + GFF211 0.89 -1.3 218,061 + -1.3 218,061 + +0.4 218,062 - +0.3 218,064 + -0.2 218,064 + -0.5 218,065 - -1.6 218,065 - -0.1 218,089 - -0.3 218,113 + -0.1 218,113 + -2.0 218,114 - -2.6 218,114 - -1.3 218,114 - +1.0 218,114 - +0.3 218,216 - -0.5 218,242 + -0.0 218,242 + -1.3 218,246 - -1.6 218,255 - +0.0 218,328 - GFF212 0.20 -0.0 218,330 + GFF212 0.20 -0.2 218,330 + GFF212 0.20 +0.7 218,330 + GFF212 0.20 +0.2 218,331 - GFF212 0.20 -0.0 218,331 - GFF212 0.20 +0.7 218,331 - GFF212 0.20 +0.6 218,384 + GFF212 0.32 -0.5 218,385 - GFF212 0.32 -1.5 218,416 + GFF212 0.39 +1.3 218,469 - GFF212 0.51 +0.4 218,469 - GFF212 0.51 -2.0 218,470 + GFF212 0.52 -0.0 218,470 + GFF212 0.52 -1.6 218,471 - GFF212 0.52 -0.0 218,471 - GFF212 0.52 -1.0 218,471 - GFF212 0.52 -0.3 218,476 - GFF212 0.53 +0.3 218,484 + GFF212 0.55 +0.3 218,484 + GFF212 0.55 +0.6 218,484 + GFF212 0.55 -0.3 218,485 - GFF212 0.55 -1.5 218,485 - GFF212 0.55 +1.8 218,493 + GFF212 0.57 -1.0 218,532 - GFF212 0.66 +0.8 218,568 + GFF212 0.74 -0.8 218,568 + GFF212 0.74 -0.0 218,569 - GFF212 0.74 -0.1 218,569 - GFF212 0.74 +0.6 218,688 + +0.2 218,688 + -0.4 218,688 + +1.3 218,689 - -1.0 218,748 + +0.2 218,749 - -0.0 218,755 + +0.1 218,755 + -0.7 218,755 + +0.8 218,755 + -0.3 218,756 - -0.3 218,756 - -0.7 218,785 + -0.0 218,787 + +0.6 218,787 + -1.0 218,787 + -3.0 218,788 - -0.0 218,788 - +1.3 218,863 - GFF213 0.14 -2.8 218,863 - GFF213 0.14 -3.2 218,863 - GFF213 0.14 -0.3 218,917 + GFF213 0.21 +0.1 218,918 - GFF213 0.21 +0.2 218,918 - GFF213 0.21 -1.0 218,918 - GFF213 0.21 -1.3 219,062 - GFF213 0.39 +0.8 219,122 + GFF213 0.46 -0.2 219,163 + GFF213 0.51 -1.0 219,164 - GFF213 0.51 +0.4 219,164 - GFF213 0.51 -0.3 219,172 - GFF213 0.52 -1.0 219,182 - GFF213 0.54 +0.8 219,229 + GFF213 0.60 -2.3 219,233 + GFF213 0.60 -0.7 219,234 - GFF213 0.60 +0.8 219,247 + GFF213 0.62 -0.3 219,412 + GFF213 0.82 -1.0 219,412 + GFF213 0.82 -0.6 219,413 - GFF213 0.82 -1.8 219,413 - GFF213 0.82 +1.3 219,457 + GFF213 0.88 -0.0 219,458 - GFF213 0.88 -1.0 219,459 + GFF213 0.88 +0.8 219,459 + GFF213 0.88 -0.4 219,460 - GFF213 0.88 +0.3 219,490 + -1.2 219,515 + -2.6 219,523 + -0.0 219,524 - -0.3 219,545 + -1.9 219,546 - -1.0 219,549 + +0.4 219,549 + +0.3 219,550 - +0.6 219,550 - -1.6 219,550 - +0.5 219,561 + +0.3 219,561 + -0.9 219,561 + -0.0 219,561 + -0.5 219,568 + +0.3 219,568 + -0.3 219,627 - -0.3 219,676 + -1.3 219,677 - -0.6 219,718 + +0.3 219,718 + +0.4 219,718 + +0.2 219,718 + -1.4 219,718 + -0.6 219,719 - +0.6 219,719 - -0.0 219,719 - +0.7 219,719 - -0.7 219,719 - +0.7 219,719 - -1.3 219,719 - -1.0 219,719 - +0.8 219,719 - -1.6 219,723 - -1.0 219,764 + -1.6 219,764 + -0.3 219,765 - +0.6 219,775 - -2.3 219,783 + -1.4 219,830 - -1.6 219,843 - -1.3 219,844 + -0.6 219,850 + -0.2 219,850 + +1.3 219,850 + -1.1 219,850 + -0.2 219,851 - -0.8 219,851 - -0.8 219,851 - -0.3 219,851 - -0.8 219,854 + -1.7 219,856 + -1.0 219,857 - +0.6 219,857 - -1.3 219,857 - +0.5 219,865 + +0.2 219,865 + -2.3 219,865 + -0.7 219,866 - -1.2 219,866 - -1.6 219,866 - -0.1 219,867 + -1.0 219,867 + -0.9 220,018 + +1.0 220,035 - GFF214 0.10 -0.9 220,085 - GFF214 0.14 +0.7 220,150 + GFF214 0.19 -1.3 220,150 + GFF214 0.19 -0.6 220,150 + GFF214 0.19 -1.2 220,150 + GFF214 0.19 -0.3 220,150 + GFF214 0.19 +0.7 220,151 - GFF214 0.19 -0.8 220,151 - GFF214 0.19 +0.2 220,151 - GFF214 0.19 -0.4 220,151 - GFF214 0.19 -0.3 220,151 - GFF214 0.19 -1.0 220,169 - GFF214 0.21 -1.0 220,174 + GFF214 0.21 -0.9 220,174 + GFF214 0.21 +0.4 220,175 - GFF214 0.21 -0.3 220,175 - GFF214 0.21 -1.0 220,175 - GFF214 0.21 +0.6 220,234 + GFF214 0.26 -0.0 220,235 - GFF214 0.26 -1.0 220,235 - GFF214 0.26 -0.5 220,235 - GFF214 0.26 -0.0 220,235 - GFF214 0.26 +0.7 220,235 - GFF214 0.26 +0.4 220,237 + GFF214 0.26 +1.6 220,238 - GFF214 0.26 -0.3 220,238 - GFF214 0.26 -0.5 220,284 + GFF214 0.30 -0.5 220,418 - GFF214 0.40 -0.6 220,432 + GFF214 0.41 +0.3 220,496 + GFF214 0.46 -0.8 220,497 - GFF214 0.46 -1.0 220,533 - GFF214 0.49 +0.6
Or see this region's nucleotide sequence