Experiment: Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2087 and GFF2088 are separated by 102 nucleotides GFF2088 and GFF2089 are separated by 4 nucleotides
GFF2087 - Cyclic di-GMP phosphodiesterase CdgJ, at 132,058 to 133,278
GFF2087
GFF2088 - Glutamine--fructose-6-phosphate aminotransferase [isomerizing], at 133,381 to 135,216
GFF2088
GFF2089 - Bifunctional protein GlmU, at 135,221 to 136,591
GFF2089
Position (kb)
133
134
135
136 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 132.393 kb on + strand, within GFF2087 at 132.393 kb on + strand, within GFF2087 at 132.447 kb on + strand, within GFF2087 at 132.447 kb on + strand, within GFF2087 at 132.447 kb on + strand, within GFF2087 at 132.451 kb on + strand, within GFF2087 at 132.451 kb on + strand, within GFF2087 at 132.451 kb on + strand, within GFF2087 at 132.452 kb on - strand, within GFF2087 at 132.452 kb on - strand, within GFF2087 at 132.452 kb on - strand, within GFF2087 at 132.452 kb on - strand, within GFF2087 at 132.468 kb on - strand, within GFF2087 at 132.551 kb on + strand, within GFF2087 at 132.551 kb on + strand, within GFF2087 at 132.565 kb on - strand, within GFF2087 at 132.565 kb on - strand, within GFF2087 at 132.567 kb on - strand, within GFF2087 at 132.567 kb on - strand, within GFF2087 at 132.688 kb on - strand, within GFF2087 at 132.711 kb on - strand, within GFF2087 at 132.729 kb on - strand, within GFF2087 at 132.826 kb on - strand, within GFF2087 at 132.864 kb on + strand, within GFF2087 at 132.902 kb on + strand, within GFF2087 at 132.938 kb on - strand, within GFF2087 at 133.045 kb on - strand, within GFF2087 at 133.126 kb on - strand, within GFF2087 at 133.152 kb on + strand, within GFF2087 at 133.209 kb on - strand at 133.257 kb on + strand at 133.257 kb on + strand at 133.312 kb on + strand at 133.376 kb on + strand at 133.376 kb on + strand at 133.376 kb on - strand at 133.377 kb on - strand at 133.377 kb on - strand at 133.377 kb on - strand at 133.382 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 1x remove 132,393 + GFF2087 0.27 +0.1 132,393 + GFF2087 0.27 -0.1 132,447 + GFF2087 0.32 -0.8 132,447 + GFF2087 0.32 +0.1 132,447 + GFF2087 0.32 -0.9 132,451 + GFF2087 0.32 +0.3 132,451 + GFF2087 0.32 -0.0 132,451 + GFF2087 0.32 -0.9 132,452 - GFF2087 0.32 -0.5 132,452 - GFF2087 0.32 -0.9 132,452 - GFF2087 0.32 +0.5 132,452 - GFF2087 0.32 -1.3 132,468 - GFF2087 0.34 -0.3 132,551 + GFF2087 0.40 -1.7 132,551 + GFF2087 0.40 -2.1 132,565 - GFF2087 0.42 -3.3 132,565 - GFF2087 0.42 -0.5 132,567 - GFF2087 0.42 +0.1 132,567 - GFF2087 0.42 -1.1 132,688 - GFF2087 0.52 +0.1 132,711 - GFF2087 0.53 -0.9 132,729 - GFF2087 0.55 +0.5 132,826 - GFF2087 0.63 -1.3 132,864 + GFF2087 0.66 +0.2 132,902 + GFF2087 0.69 -0.9 132,938 - GFF2087 0.72 -0.7 133,045 - GFF2087 0.81 -0.9 133,126 - GFF2087 0.87 +0.0 133,152 + GFF2087 0.90 -0.2 133,209 - -0.4 133,257 + +0.3 133,257 + +0.1 133,312 + -0.6 133,376 + -1.5 133,376 + -0.1 133,376 - -0.8 133,377 - +0.7 133,377 - -1.5 133,377 - +0.3 133,382 + -0.5
Or see this region's nucleotide sequence