Experiment: Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2068 and GFF2069 are separated by 12 nucleotides GFF2069 and GFF2070 are separated by 31 nucleotides GFF2070 and GFF2071 are separated by 15 nucleotides
GFF2068 - Biosynthetic peptidoglycan transglycosylase, at 108,607 to 109,893
GFF2068
GFF2069 - Ferredoxin, 2Fe-2S, at 109,906 to 110,220
GFF2069
GFF2070 - hypothetical protein, at 110,252 to 111,205
GFF2070
GFF2071 - UDP-N-acetylmuramate--L-alanyl-gamma-D-glutamyl-m eso-2,6-diaminoheptandioate ligase, at 111,221 to 112,591
GFF2071
Position (kb)
110
111
112 Strain fitness (log2 ratio)
-2
-1
0
1 at 109.411 kb on - strand, within GFF2068 at 109.411 kb on - strand, within GFF2068 at 109.411 kb on - strand, within GFF2068 at 109.556 kb on - strand, within GFF2068 at 109.556 kb on - strand, within GFF2068 at 109.584 kb on + strand, within GFF2068 at 109.599 kb on + strand, within GFF2068 at 109.600 kb on - strand, within GFF2068 at 109.600 kb on - strand, within GFF2068 at 109.606 kb on + strand, within GFF2068 at 109.649 kb on + strand, within GFF2068 at 109.649 kb on + strand, within GFF2068 at 109.649 kb on + strand, within GFF2068 at 109.649 kb on + strand, within GFF2068 at 109.649 kb on + strand, within GFF2068 at 109.649 kb on + strand, within GFF2068 at 109.649 kb on + strand, within GFF2068 at 109.650 kb on - strand, within GFF2068 at 109.650 kb on - strand, within GFF2068 at 109.650 kb on - strand, within GFF2068 at 109.650 kb on - strand, within GFF2068 at 109.677 kb on + strand, within GFF2068 at 109.678 kb on - strand, within GFF2068 at 109.777 kb on + strand at 109.777 kb on + strand at 109.795 kb on - strand at 109.800 kb on - strand at 109.865 kb on - strand at 109.865 kb on - strand at 109.907 kb on + strand at 109.907 kb on + strand at 109.907 kb on + strand at 109.907 kb on + strand at 109.908 kb on - strand at 109.908 kb on - strand at 109.908 kb on - strand at 109.908 kb on - strand at 109.908 kb on - strand at 109.908 kb on - strand at 109.989 kb on + strand, within GFF2069 at 109.989 kb on + strand, within GFF2069 at 109.990 kb on - strand, within GFF2069 at 109.990 kb on - strand, within GFF2069 at 109.990 kb on - strand, within GFF2069 at 109.990 kb on - strand, within GFF2069 at 109.990 kb on - strand, within GFF2069 at 109.990 kb on - strand, within GFF2069 at 110.117 kb on + strand, within GFF2069 at 110.149 kb on - strand, within GFF2069 at 110.149 kb on - strand, within GFF2069 at 110.199 kb on - strand at 110.223 kb on - strand at 110.223 kb on - strand at 110.235 kb on + strand at 110.240 kb on - strand at 110.240 kb on - strand at 110.253 kb on + strand at 110.254 kb on - strand at 110.282 kb on - strand at 110.299 kb on + strand at 110.300 kb on - strand at 110.300 kb on - strand at 110.302 kb on + strand at 110.302 kb on + strand at 110.302 kb on + strand at 110.302 kb on + strand at 110.303 kb on - strand at 110.303 kb on - strand at 110.330 kb on - strand at 110.330 kb on - strand at 110.419 kb on + strand, within GFF2070 at 110.420 kb on - strand, within GFF2070 at 110.425 kb on + strand, within GFF2070 at 110.426 kb on - strand, within GFF2070 at 110.426 kb on - strand, within GFF2070 at 110.433 kb on - strand, within GFF2070 at 110.478 kb on + strand, within GFF2070 at 110.479 kb on - strand, within GFF2070 at 110.479 kb on - strand, within GFF2070 at 110.479 kb on - strand, within GFF2070 at 110.479 kb on - strand, within GFF2070 at 110.479 kb on - strand, within GFF2070 at 110.479 kb on - strand, within GFF2070 at 110.480 kb on + strand, within GFF2070 at 110.480 kb on + strand, within GFF2070 at 110.481 kb on - strand, within GFF2070 at 110.481 kb on - strand, within GFF2070 at 110.509 kb on - strand, within GFF2070 at 110.556 kb on + strand, within GFF2070 at 110.557 kb on - strand, within GFF2070 at 110.557 kb on - strand, within GFF2070 at 110.572 kb on - strand, within GFF2070 at 110.619 kb on + strand, within GFF2070 at 110.619 kb on + strand, within GFF2070 at 110.768 kb on - strand, within GFF2070 at 110.779 kb on + strand, within GFF2070 at 110.780 kb on - strand, within GFF2070 at 110.790 kb on - strand, within GFF2070 at 110.801 kb on + strand, within GFF2070 at 110.930 kb on + strand, within GFF2070 at 110.930 kb on + strand, within GFF2070 at 110.930 kb on + strand, within GFF2070 at 110.930 kb on + strand, within GFF2070 at 110.931 kb on - strand, within GFF2070 at 110.931 kb on - strand, within GFF2070 at 111.088 kb on + strand, within GFF2070 at 111.129 kb on - strand at 111.144 kb on - strand at 111.222 kb on + strand at 111.243 kb on - strand at 111.243 kb on - strand at 111.725 kb on - strand, within GFF2071 at 111.759 kb on - strand, within GFF2071
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 1x remove 109,411 - GFF2068 0.62 +0.8 109,411 - GFF2068 0.62 -0.3 109,411 - GFF2068 0.62 -0.9 109,556 - GFF2068 0.74 -1.3 109,556 - GFF2068 0.74 -0.2 109,584 + GFF2068 0.76 +0.1 109,599 + GFF2068 0.77 -0.8 109,600 - GFF2068 0.77 -1.3 109,600 - GFF2068 0.77 -1.1 109,606 + GFF2068 0.78 -0.4 109,649 + GFF2068 0.81 -0.5 109,649 + GFF2068 0.81 -0.9 109,649 + GFF2068 0.81 +0.2 109,649 + GFF2068 0.81 -0.5 109,649 + GFF2068 0.81 -0.4 109,649 + GFF2068 0.81 -0.5 109,649 + GFF2068 0.81 -0.5 109,650 - GFF2068 0.81 +0.1 109,650 - GFF2068 0.81 +0.1 109,650 - GFF2068 0.81 -0.1 109,650 - GFF2068 0.81 -1.5 109,677 + GFF2068 0.83 -0.3 109,678 - GFF2068 0.83 -2.3 109,777 + -1.3 109,777 + -1.1 109,795 - -0.1 109,800 - -0.1 109,865 - +0.6 109,865 - -0.2 109,907 + -1.3 109,907 + -0.4 109,907 + +0.6 109,907 + +0.4 109,908 - -0.9 109,908 - -1.9 109,908 - +0.3 109,908 - -1.1 109,908 - +0.6 109,908 - -0.9 109,989 + GFF2069 0.26 +0.1 109,989 + GFF2069 0.26 -1.3 109,990 - GFF2069 0.27 +0.1 109,990 - GFF2069 0.27 -1.8 109,990 - GFF2069 0.27 -1.9 109,990 - GFF2069 0.27 -0.8 109,990 - GFF2069 0.27 -0.3 109,990 - GFF2069 0.27 -0.7 110,117 + GFF2069 0.67 -1.3 110,149 - GFF2069 0.77 -1.3 110,149 - GFF2069 0.77 -0.9 110,199 - -0.5 110,223 - +1.1 110,223 - -0.1 110,235 + +0.3 110,240 - -0.2 110,240 - -0.9 110,253 + -1.5 110,254 - -2.3 110,282 - -0.3 110,299 + +1.1 110,300 - -0.0 110,300 - -0.7 110,302 + +0.1 110,302 + +0.6 110,302 + +0.9 110,302 + +0.4 110,303 - +0.3 110,303 - -0.3 110,330 - +0.9 110,330 - -0.4 110,419 + GFF2070 0.18 -1.5 110,420 - GFF2070 0.18 +0.4 110,425 + GFF2070 0.18 -2.5 110,426 - GFF2070 0.18 -2.1 110,426 - GFF2070 0.18 +0.3 110,433 - GFF2070 0.19 -0.2 110,478 + GFF2070 0.24 -0.7 110,479 - GFF2070 0.24 +0.7 110,479 - GFF2070 0.24 -2.4 110,479 - GFF2070 0.24 -0.3 110,479 - GFF2070 0.24 -0.5 110,479 - GFF2070 0.24 -0.9 110,479 - GFF2070 0.24 -0.6 110,480 + GFF2070 0.24 -1.3 110,480 + GFF2070 0.24 -0.7 110,481 - GFF2070 0.24 -0.3 110,481 - GFF2070 0.24 -0.8 110,509 - GFF2070 0.27 +1.1 110,556 + GFF2070 0.32 +0.4 110,557 - GFF2070 0.32 +1.3 110,557 - GFF2070 0.32 -1.5 110,572 - GFF2070 0.34 -1.3 110,619 + GFF2070 0.38 +0.1 110,619 + GFF2070 0.38 -1.6 110,768 - GFF2070 0.54 +0.8 110,779 + GFF2070 0.55 +0.5 110,780 - GFF2070 0.55 +0.9 110,790 - GFF2070 0.56 -0.5 110,801 + GFF2070 0.58 -1.3 110,930 + GFF2070 0.71 +0.5 110,930 + GFF2070 0.71 -1.3 110,930 + GFF2070 0.71 +0.2 110,930 + GFF2070 0.71 -0.4 110,931 - GFF2070 0.71 -1.4 110,931 - GFF2070 0.71 -0.3 111,088 + GFF2070 0.88 -1.3 111,129 - +0.2 111,144 - -0.8 111,222 + -0.9 111,243 - -0.4 111,243 - +0.1 111,725 - GFF2071 0.37 -0.9 111,759 - GFF2071 0.39 +0.6
Or see this region's nucleotide sequence