Experiment: Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2056 and GFF2057 are separated by 42 nucleotides GFF2057 and GFF2058 are separated by 52 nucleotides GFF2058 and GFF2059 overlap by 4 nucleotides GFF2059 and GFF2060 are separated by 19 nucleotides
GFF2056 - Release factor glutamine methyltransferase, at 95,186 to 96,058
GFF2056
GFF2057 - Sugar-phosphatase AraL, at 96,101 to 96,904
GFF2057
GFF2058 - Homoserine O-succinyltransferase, at 96,957 to 98,084
GFF2058
GFF2059 - Bifunctional methionine biosynthesis protein MetXA/MetW, at 98,081 to 98,686
GFF2059
GFF2060 - Acetoin catabolism regulatory protein, at 98,706 to 100,721
GFF2060
Position (kb) 96
97
98
99 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 96.042 kb on + strand at 96.042 kb on + strand at 96.055 kb on - strand at 96.085 kb on + strand at 96.085 kb on + strand at 96.085 kb on + strand at 96.085 kb on + strand at 96.090 kb on - strand at 96.090 kb on - strand at 96.090 kb on - strand at 96.090 kb on - strand at 96.110 kb on - strand at 96.118 kb on + strand at 96.118 kb on + strand at 96.119 kb on - strand at 96.121 kb on + strand at 96.121 kb on + strand at 96.122 kb on - strand at 96.132 kb on + strand at 96.132 kb on + strand at 96.133 kb on - strand at 96.155 kb on + strand at 96.155 kb on + strand at 96.155 kb on + strand at 96.155 kb on + strand at 96.155 kb on + strand at 96.156 kb on - strand at 96.156 kb on - strand at 96.156 kb on - strand at 96.156 kb on - strand at 96.156 kb on - strand at 96.236 kb on - strand, within GFF2057 at 96.244 kb on + strand, within GFF2057 at 96.244 kb on + strand, within GFF2057 at 96.244 kb on + strand, within GFF2057 at 96.245 kb on - strand, within GFF2057 at 96.245 kb on - strand, within GFF2057 at 96.288 kb on + strand, within GFF2057 at 96.288 kb on + strand, within GFF2057 at 96.300 kb on + strand, within GFF2057 at 96.300 kb on + strand, within GFF2057 at 96.344 kb on + strand, within GFF2057 at 96.344 kb on + strand, within GFF2057 at 96.374 kb on - strand, within GFF2057 at 96.443 kb on + strand, within GFF2057 at 96.444 kb on - strand, within GFF2057 at 96.462 kb on + strand, within GFF2057 at 96.462 kb on + strand, within GFF2057 at 96.462 kb on + strand, within GFF2057 at 96.462 kb on + strand, within GFF2057 at 96.463 kb on - strand, within GFF2057 at 96.479 kb on + strand, within GFF2057 at 96.479 kb on + strand, within GFF2057 at 96.479 kb on + strand, within GFF2057 at 96.479 kb on + strand, within GFF2057 at 96.480 kb on - strand, within GFF2057 at 96.486 kb on + strand, within GFF2057 at 96.486 kb on + strand, within GFF2057 at 96.486 kb on + strand, within GFF2057 at 96.487 kb on - strand, within GFF2057 at 96.487 kb on - strand, within GFF2057 at 96.487 kb on - strand, within GFF2057 at 96.528 kb on + strand, within GFF2057 at 96.541 kb on + strand, within GFF2057 at 96.583 kb on + strand, within GFF2057 at 96.584 kb on - strand, within GFF2057 at 96.623 kb on - strand, within GFF2057 at 96.636 kb on + strand, within GFF2057 at 96.637 kb on - strand, within GFF2057 at 96.667 kb on + strand, within GFF2057 at 96.667 kb on + strand, within GFF2057 at 96.668 kb on - strand, within GFF2057 at 96.684 kb on + strand, within GFF2057 at 96.685 kb on - strand, within GFF2057 at 96.724 kb on + strand, within GFF2057 at 96.725 kb on - strand, within GFF2057 at 96.725 kb on - strand, within GFF2057 at 96.792 kb on - strand, within GFF2057 at 96.803 kb on + strand, within GFF2057 at 96.803 kb on + strand, within GFF2057 at 96.803 kb on + strand, within GFF2057 at 96.803 kb on + strand, within GFF2057 at 96.804 kb on - strand, within GFF2057 at 96.804 kb on - strand, within GFF2057 at 96.814 kb on - strand, within GFF2057 at 96.870 kb on + strand at 96.870 kb on + strand at 96.870 kb on + strand at 96.870 kb on + strand at 96.870 kb on + strand at 96.870 kb on + strand at 96.871 kb on - strand at 96.943 kb on - strand at 96.945 kb on + strand at 96.945 kb on + strand at 96.945 kb on + strand at 96.945 kb on + strand at 96.946 kb on - strand at 96.946 kb on - strand at 96.946 kb on - strand at 98.095 kb on + strand at 98.095 kb on + strand at 98.098 kb on + strand at 98.124 kb on + strand at 98.124 kb on + strand at 98.166 kb on + strand, within GFF2059 at 98.166 kb on + strand, within GFF2059 at 98.166 kb on + strand, within GFF2059 at 98.166 kb on + strand, within GFF2059 at 98.211 kb on + strand, within GFF2059 at 98.218 kb on + strand, within GFF2059 at 98.219 kb on - strand, within GFF2059 at 98.338 kb on + strand, within GFF2059 at 98.338 kb on + strand, within GFF2059 at 98.338 kb on + strand, within GFF2059 at 98.338 kb on + strand, within GFF2059 at 98.339 kb on - strand, within GFF2059 at 98.339 kb on - strand, within GFF2059 at 98.377 kb on + strand, within GFF2059 at 98.378 kb on - strand, within GFF2059 at 98.409 kb on + strand, within GFF2059 at 98.409 kb on + strand, within GFF2059 at 98.507 kb on + strand, within GFF2059 at 98.516 kb on + strand, within GFF2059 at 98.517 kb on - strand, within GFF2059 at 98.538 kb on + strand, within GFF2059 at 98.538 kb on + strand, within GFF2059 at 98.538 kb on + strand, within GFF2059 at 98.538 kb on + strand, within GFF2059 at 98.538 kb on + strand, within GFF2059 at 98.538 kb on + strand, within GFF2059 at 98.538 kb on + strand, within GFF2059 at 98.538 kb on + strand, within GFF2059 at 98.538 kb on + strand, within GFF2059 at 98.538 kb on + strand, within GFF2059 at 98.538 kb on + strand, within GFF2059 at 98.539 kb on - strand, within GFF2059 at 98.539 kb on - strand, within GFF2059 at 98.539 kb on - strand, within GFF2059 at 98.539 kb on - strand, within GFF2059 at 98.539 kb on - strand, within GFF2059 at 98.539 kb on - strand, within GFF2059 at 98.539 kb on - strand, within GFF2059 at 98.539 kb on - strand, within GFF2059 at 98.539 kb on - strand, within GFF2059 at 98.604 kb on + strand, within GFF2059 at 98.604 kb on + strand, within GFF2059 at 98.604 kb on + strand, within GFF2059 at 98.604 kb on + strand, within GFF2059 at 98.604 kb on + strand, within GFF2059 at 98.604 kb on + strand, within GFF2059 at 98.604 kb on + strand, within GFF2059 at 98.605 kb on - strand, within GFF2059 at 98.605 kb on - strand, within GFF2059 at 98.605 kb on - strand, within GFF2059 at 98.609 kb on - strand, within GFF2059 at 98.663 kb on + strand at 98.663 kb on + strand at 98.663 kb on + strand at 98.663 kb on + strand at 98.664 kb on - strand at 98.670 kb on + strand at 98.670 kb on + strand at 98.671 kb on - strand at 98.727 kb on + strand at 98.727 kb on + strand at 98.728 kb on - strand at 98.728 kb on - strand at 98.728 kb on - strand at 98.738 kb on - strand at 98.745 kb on - strand at 98.783 kb on + strand at 98.783 kb on + strand at 98.783 kb on + strand at 98.784 kb on - strand at 98.784 kb on - strand at 98.987 kb on + strand, within GFF2060 at 98.987 kb on + strand, within GFF2060 at 98.987 kb on + strand, within GFF2060 at 98.987 kb on + strand, within GFF2060 at 98.988 kb on - strand, within GFF2060 at 98.988 kb on - strand, within GFF2060 at 98.991 kb on + strand, within GFF2060 at 98.991 kb on + strand, within GFF2060 at 98.992 kb on - strand, within GFF2060 at 99.000 kb on + strand, within GFF2060 at 99.000 kb on + strand, within GFF2060 at 99.001 kb on - strand, within GFF2060
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 1x remove 96,042 + -0.5 96,042 + +0.5 96,055 - -0.9 96,085 + -0.2 96,085 + -1.3 96,085 + +0.9 96,085 + -0.7 96,090 - -2.6 96,090 - -0.0 96,090 - -1.4 96,090 - +0.1 96,110 - +0.3 96,118 + -1.5 96,118 + -1.5 96,119 - -0.3 96,121 + -1.3 96,121 + +0.1 96,122 - -1.3 96,132 + +1.6 96,132 + +0.6 96,133 - -1.5 96,155 + +1.2 96,155 + +0.2 96,155 + -1.0 96,155 + +0.1 96,155 + -0.6 96,156 - +0.6 96,156 - -0.3 96,156 - -1.1 96,156 - -1.8 96,156 - -0.3 96,236 - GFF2057 0.17 +0.1 96,244 + GFF2057 0.18 +0.5 96,244 + GFF2057 0.18 +0.4 96,244 + GFF2057 0.18 -1.3 96,245 - GFF2057 0.18 -2.1 96,245 - GFF2057 0.18 -1.3 96,288 + GFF2057 0.23 -1.1 96,288 + GFF2057 0.23 +0.6 96,300 + GFF2057 0.25 -1.5 96,300 + GFF2057 0.25 -0.5 96,344 + GFF2057 0.30 +0.6 96,344 + GFF2057 0.30 +0.7 96,374 - GFF2057 0.34 -0.9 96,443 + GFF2057 0.43 -0.6 96,444 - GFF2057 0.43 -1.9 96,462 + GFF2057 0.45 +0.2 96,462 + GFF2057 0.45 +0.5 96,462 + GFF2057 0.45 -2.6 96,462 + GFF2057 0.45 -1.3 96,463 - GFF2057 0.45 -0.3 96,479 + GFF2057 0.47 -1.5 96,479 + GFF2057 0.47 -0.8 96,479 + GFF2057 0.47 +1.4 96,479 + GFF2057 0.47 +0.1 96,480 - GFF2057 0.47 -0.4 96,486 + GFF2057 0.48 -0.8 96,486 + GFF2057 0.48 -1.3 96,486 + GFF2057 0.48 -1.5 96,487 - GFF2057 0.48 +0.1 96,487 - GFF2057 0.48 -1.3 96,487 - GFF2057 0.48 -1.9 96,528 + GFF2057 0.53 -1.1 96,541 + GFF2057 0.55 -0.8 96,583 + GFF2057 0.60 -0.8 96,584 - GFF2057 0.60 -0.1 96,623 - GFF2057 0.65 -1.1 96,636 + GFF2057 0.67 +1.1 96,637 - GFF2057 0.67 -1.3 96,667 + GFF2057 0.70 -1.0 96,667 + GFF2057 0.70 +0.1 96,668 - GFF2057 0.71 +0.1 96,684 + GFF2057 0.73 -1.9 96,685 - GFF2057 0.73 -1.5 96,724 + GFF2057 0.77 -1.3 96,725 - GFF2057 0.78 -0.8 96,725 - GFF2057 0.78 -0.5 96,792 - GFF2057 0.86 +0.6 96,803 + GFF2057 0.87 -1.3 96,803 + GFF2057 0.87 -1.8 96,803 + GFF2057 0.87 +0.7 96,803 + GFF2057 0.87 +0.1 96,804 - GFF2057 0.87 -0.1 96,804 - GFF2057 0.87 -0.3 96,814 - GFF2057 0.89 -0.1 96,870 + -1.9 96,870 + -1.1 96,870 + -0.2 96,870 + +0.7 96,870 + -0.3 96,870 + +0.4 96,871 - -0.5 96,943 - -1.3 96,945 + -0.9 96,945 + -1.3 96,945 + -0.2 96,945 + -0.8 96,946 - +0.2 96,946 - -0.6 96,946 - +0.6 98,095 + +0.1 98,095 + -1.0 98,098 + -0.5 98,124 + +1.1 98,124 + -0.9 98,166 + GFF2059 0.14 -1.1 98,166 + GFF2059 0.14 -0.1 98,166 + GFF2059 0.14 -0.1 98,166 + GFF2059 0.14 +1.3 98,211 + GFF2059 0.21 -0.5 98,218 + GFF2059 0.23 -1.1 98,219 - GFF2059 0.23 +0.1 98,338 + GFF2059 0.42 -0.4 98,338 + GFF2059 0.42 -1.3 98,338 + GFF2059 0.42 +1.1 98,338 + GFF2059 0.42 -0.1 98,339 - GFF2059 0.43 -0.7 98,339 - GFF2059 0.43 -0.4 98,377 + GFF2059 0.49 -1.4 98,378 - GFF2059 0.49 +1.1 98,409 + GFF2059 0.54 +0.4 98,409 + GFF2059 0.54 +0.4 98,507 + GFF2059 0.70 -0.8 98,516 + GFF2059 0.72 -0.5 98,517 - GFF2059 0.72 -0.3 98,538 + GFF2059 0.75 -1.0 98,538 + GFF2059 0.75 -0.4 98,538 + GFF2059 0.75 -0.3 98,538 + GFF2059 0.75 -0.7 98,538 + GFF2059 0.75 -0.7 98,538 + GFF2059 0.75 -0.5 98,538 + GFF2059 0.75 -1.4 98,538 + GFF2059 0.75 +0.3 98,538 + GFF2059 0.75 -0.7 98,538 + GFF2059 0.75 -0.5 98,538 + GFF2059 0.75 +0.3 98,539 - GFF2059 0.76 -0.9 98,539 - GFF2059 0.76 +0.8 98,539 - GFF2059 0.76 -0.7 98,539 - GFF2059 0.76 +1.2 98,539 - GFF2059 0.76 -0.4 98,539 - GFF2059 0.76 -1.1 98,539 - GFF2059 0.76 -1.3 98,539 - GFF2059 0.76 -1.5 98,539 - GFF2059 0.76 -2.8 98,604 + GFF2059 0.86 +0.5 98,604 + GFF2059 0.86 +1.1 98,604 + GFF2059 0.86 +0.7 98,604 + GFF2059 0.86 -0.3 98,604 + GFF2059 0.86 -0.2 98,604 + GFF2059 0.86 +0.1 98,604 + GFF2059 0.86 -0.3 98,605 - GFF2059 0.86 +0.2 98,605 - GFF2059 0.86 -0.2 98,605 - GFF2059 0.86 -0.0 98,609 - GFF2059 0.87 -0.4 98,663 + -0.7 98,663 + -0.3 98,663 + -0.2 98,663 + -0.1 98,664 - -1.8 98,670 + -1.0 98,670 + -2.3 98,671 - +0.7 98,727 + -0.2 98,727 + -0.3 98,728 - -0.4 98,728 - -1.9 98,728 - -0.7 98,738 - +0.1 98,745 - +0.5 98,783 + -0.3 98,783 + +0.1 98,783 + -1.2 98,784 - +0.4 98,784 - +0.1 98,987 + GFF2060 0.14 -0.0 98,987 + GFF2060 0.14 -0.9 98,987 + GFF2060 0.14 -0.8 98,987 + GFF2060 0.14 -0.9 98,988 - GFF2060 0.14 -0.2 98,988 - GFF2060 0.14 -0.6 98,991 + GFF2060 0.14 -0.6 98,991 + GFF2060 0.14 -0.2 98,992 - GFF2060 0.14 -0.5 99,000 + GFF2060 0.15 -0.1 99,000 + GFF2060 0.15 -0.9 99,001 - GFF2060 0.15 +0.1
Or see this region's nucleotide sequence