Experiment: Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1983 and GFF1984 are separated by 32 nucleotides GFF1984 and GFF1985 overlap by 6 nucleotides GFF1985 and GFF1986 are separated by 8 nucleotides
GFF1983 - hypothetical protein, at 20,919 to 21,779
GFF1983
GFF1984 - hypothetical protein, at 21,812 to 21,943
GFF1984
GFF1985 - putative FMNH2-dependent monooxygenase SfnC, at 21,938 to 23,158
GFF1985
GFF1986 - Methanesulfonate monooxygenase, at 23,167 to 24,300
GFF1986
Position (kb)
21
22
23
24 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 20.962 kb on + strand at 20.979 kb on + strand at 21.097 kb on + strand, within GFF1983 at 21.097 kb on + strand, within GFF1983 at 21.097 kb on + strand, within GFF1983 at 21.098 kb on - strand, within GFF1983 at 21.124 kb on + strand, within GFF1983 at 21.124 kb on + strand, within GFF1983 at 21.124 kb on + strand, within GFF1983 at 21.125 kb on - strand, within GFF1983 at 21.125 kb on - strand, within GFF1983 at 21.125 kb on - strand, within GFF1983 at 21.128 kb on + strand, within GFF1983 at 21.142 kb on + strand, within GFF1983 at 21.213 kb on - strand, within GFF1983 at 21.213 kb on - strand, within GFF1983 at 21.213 kb on - strand, within GFF1983 at 21.229 kb on + strand, within GFF1983 at 21.319 kb on + strand, within GFF1983 at 21.337 kb on + strand, within GFF1983 at 21.338 kb on - strand, within GFF1983 at 21.338 kb on - strand, within GFF1983 at 21.338 kb on - strand, within GFF1983 at 21.338 kb on - strand, within GFF1983 at 21.418 kb on + strand, within GFF1983 at 21.418 kb on + strand, within GFF1983 at 21.426 kb on + strand, within GFF1983 at 21.603 kb on + strand, within GFF1983 at 21.616 kb on + strand, within GFF1983 at 21.616 kb on + strand, within GFF1983 at 21.617 kb on - strand, within GFF1983 at 21.624 kb on + strand, within GFF1983 at 21.624 kb on + strand, within GFF1983 at 21.631 kb on + strand, within GFF1983 at 21.631 kb on + strand, within GFF1983 at 21.632 kb on - strand, within GFF1983 at 21.697 kb on + strand at 21.698 kb on - strand at 21.756 kb on + strand at 21.756 kb on + strand at 21.757 kb on - strand at 21.779 kb on + strand at 21.779 kb on + strand at 21.779 kb on + strand at 21.779 kb on + strand at 21.780 kb on - strand at 21.783 kb on + strand at 21.783 kb on + strand at 21.783 kb on + strand at 21.783 kb on + strand at 21.784 kb on - strand at 21.784 kb on - strand at 21.806 kb on + strand at 21.806 kb on + strand at 21.807 kb on - strand at 21.807 kb on - strand at 21.807 kb on - strand at 21.807 kb on - strand at 21.807 kb on - strand at 21.807 kb on - strand at 21.807 kb on - strand at 21.807 kb on - strand at 21.807 kb on - strand at 21.863 kb on + strand, within GFF1984 at 21.863 kb on + strand, within GFF1984 at 21.863 kb on + strand, within GFF1984 at 21.863 kb on + strand, within GFF1984 at 21.863 kb on + strand, within GFF1984 at 21.864 kb on - strand, within GFF1984 at 21.864 kb on - strand, within GFF1984 at 21.864 kb on - strand, within GFF1984 at 21.864 kb on - strand, within GFF1984 at 21.864 kb on - strand, within GFF1984 at 21.872 kb on + strand, within GFF1984 at 21.872 kb on + strand, within GFF1984 at 21.872 kb on + strand, within GFF1984 at 21.872 kb on + strand, within GFF1984 at 21.872 kb on + strand, within GFF1984 at 21.872 kb on + strand, within GFF1984 at 21.872 kb on + strand, within GFF1984 at 21.872 kb on + strand, within GFF1984 at 21.872 kb on + strand, within GFF1984 at 21.873 kb on - strand, within GFF1984 at 21.873 kb on - strand, within GFF1984 at 21.873 kb on - strand, within GFF1984 at 21.873 kb on - strand, within GFF1984 at 21.873 kb on - strand, within GFF1984 at 21.939 kb on + strand at 21.939 kb on + strand at 21.940 kb on - strand at 21.946 kb on - strand at 21.946 kb on - strand at 21.946 kb on - strand at 21.953 kb on - strand at 22.006 kb on - strand at 22.027 kb on + strand at 22.028 kb on - strand at 22.029 kb on + strand at 22.029 kb on + strand at 22.029 kb on + strand at 22.029 kb on + strand at 22.029 kb on + strand at 22.030 kb on - strand at 22.030 kb on - strand at 22.030 kb on - strand at 22.030 kb on - strand at 22.030 kb on - strand at 22.030 kb on - strand at 22.030 kb on - strand at 22.030 kb on - strand at 22.030 kb on - strand at 22.030 kb on - strand at 22.030 kb on - strand at 22.030 kb on - strand at 22.251 kb on - strand, within GFF1985 at 22.265 kb on + strand, within GFF1985 at 22.265 kb on + strand, within GFF1985 at 22.265 kb on + strand, within GFF1985 at 22.266 kb on - strand, within GFF1985 at 22.287 kb on + strand, within GFF1985 at 22.300 kb on + strand, within GFF1985 at 22.300 kb on + strand, within GFF1985 at 22.300 kb on + strand, within GFF1985 at 22.301 kb on - strand, within GFF1985 at 22.301 kb on - strand, within GFF1985 at 22.301 kb on - strand, within GFF1985 at 22.301 kb on - strand, within GFF1985 at 22.301 kb on - strand, within GFF1985 at 22.302 kb on + strand, within GFF1985 at 22.303 kb on - strand, within GFF1985 at 22.315 kb on - strand, within GFF1985 at 22.321 kb on + strand, within GFF1985 at 22.321 kb on + strand, within GFF1985 at 22.321 kb on + strand, within GFF1985 at 22.321 kb on + strand, within GFF1985 at 22.322 kb on - strand, within GFF1985 at 22.322 kb on - strand, within GFF1985 at 22.322 kb on - strand, within GFF1985 at 22.365 kb on + strand, within GFF1985 at 22.407 kb on + strand, within GFF1985 at 22.408 kb on - strand, within GFF1985 at 22.408 kb on - strand, within GFF1985 at 22.411 kb on + strand, within GFF1985 at 22.411 kb on + strand, within GFF1985 at 22.411 kb on + strand, within GFF1985 at 22.411 kb on + strand, within GFF1985 at 22.411 kb on + strand, within GFF1985 at 22.412 kb on - strand, within GFF1985 at 22.418 kb on - strand, within GFF1985 at 22.418 kb on - strand, within GFF1985 at 22.487 kb on + strand, within GFF1985 at 22.488 kb on - strand, within GFF1985 at 22.488 kb on - strand, within GFF1985 at 22.488 kb on - strand, within GFF1985 at 22.488 kb on - strand, within GFF1985 at 22.488 kb on - strand, within GFF1985 at 22.488 kb on - strand, within GFF1985 at 22.555 kb on + strand, within GFF1985 at 22.638 kb on - strand, within GFF1985 at 22.638 kb on - strand, within GFF1985 at 22.707 kb on + strand, within GFF1985 at 22.707 kb on + strand, within GFF1985 at 22.708 kb on - strand, within GFF1985 at 22.718 kb on + strand, within GFF1985 at 22.719 kb on - strand, within GFF1985 at 22.800 kb on + strand, within GFF1985 at 22.801 kb on - strand, within GFF1985 at 22.801 kb on - strand, within GFF1985 at 22.827 kb on + strand, within GFF1985 at 22.829 kb on + strand, within GFF1985 at 22.829 kb on + strand, within GFF1985 at 22.829 kb on + strand, within GFF1985 at 22.829 kb on + strand, within GFF1985 at 22.829 kb on + strand, within GFF1985 at 22.830 kb on - strand, within GFF1985 at 22.830 kb on - strand, within GFF1985 at 23.010 kb on + strand, within GFF1985 at 23.010 kb on + strand, within GFF1985 at 23.011 kb on - strand, within GFF1985 at 23.223 kb on + strand at 23.224 kb on - strand at 23.224 kb on - strand at 23.230 kb on - strand at 23.275 kb on + strand at 23.326 kb on - strand, within GFF1986 at 23.359 kb on - strand, within GFF1986 at 23.723 kb on - strand, within GFF1986 at 23.723 kb on - strand, within GFF1986 at 23.723 kb on - strand, within GFF1986 at 23.736 kb on + strand, within GFF1986 at 23.736 kb on + strand, within GFF1986 at 23.737 kb on - strand, within GFF1986 at 23.737 kb on - strand, within GFF1986 at 23.737 kb on - strand, within GFF1986 at 23.737 kb on - strand, within GFF1986 at 23.737 kb on - strand, within GFF1986 at 23.737 kb on - strand, within GFF1986 at 23.842 kb on + strand, within GFF1986 at 23.842 kb on + strand, within GFF1986 at 23.842 kb on + strand, within GFF1986 at 23.846 kb on + strand, within GFF1986 at 23.847 kb on - strand, within GFF1986 at 23.882 kb on - strand, within GFF1986 at 23.895 kb on + strand, within GFF1986 at 23.991 kb on + strand, within GFF1986 at 23.992 kb on - strand, within GFF1986 at 23.992 kb on - strand, within GFF1986 at 24.061 kb on - strand, within GFF1986
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Methylosinus sp. DMC45 grown in dNMS with methane 1x remove 20,962 + +1.9 20,979 + -0.1 21,097 + GFF1983 0.21 -0.9 21,097 + GFF1983 0.21 +0.4 21,097 + GFF1983 0.21 -0.1 21,098 - GFF1983 0.21 -2.6 21,124 + GFF1983 0.24 -0.8 21,124 + GFF1983 0.24 -1.8 21,124 + GFF1983 0.24 -0.9 21,125 - GFF1983 0.24 -0.8 21,125 - GFF1983 0.24 -0.5 21,125 - GFF1983 0.24 -0.5 21,128 + GFF1983 0.24 +0.3 21,142 + GFF1983 0.26 -0.5 21,213 - GFF1983 0.34 -0.1 21,213 - GFF1983 0.34 -0.1 21,213 - GFF1983 0.34 -1.6 21,229 + GFF1983 0.36 +0.2 21,319 + GFF1983 0.46 -0.5 21,337 + GFF1983 0.49 -0.9 21,338 - GFF1983 0.49 -0.8 21,338 - GFF1983 0.49 -1.8 21,338 - GFF1983 0.49 -0.9 21,338 - GFF1983 0.49 -0.8 21,418 + GFF1983 0.58 +1.1 21,418 + GFF1983 0.58 +1.3 21,426 + GFF1983 0.59 -0.1 21,603 + GFF1983 0.79 +1.6 21,616 + GFF1983 0.81 -0.5 21,616 + GFF1983 0.81 -0.1 21,617 - GFF1983 0.81 -0.7 21,624 + GFF1983 0.82 -0.3 21,624 + GFF1983 0.82 +0.2 21,631 + GFF1983 0.83 -1.1 21,631 + GFF1983 0.83 -0.9 21,632 - GFF1983 0.83 -0.5 21,697 + -0.2 21,698 - +0.8 21,756 + -1.6 21,756 + -1.5 21,757 - +0.4 21,779 + -0.8 21,779 + -0.6 21,779 + -0.5 21,779 + -0.5 21,780 - +0.1 21,783 + -0.5 21,783 + -1.6 21,783 + -1.1 21,783 + +0.4 21,784 - +0.4 21,784 - -0.8 21,806 + +0.6 21,806 + +0.3 21,807 - +0.1 21,807 - -0.8 21,807 - -1.3 21,807 - -0.7 21,807 - -0.9 21,807 - -1.5 21,807 - -0.2 21,807 - -0.8 21,807 - -0.3 21,863 + GFF1984 0.39 -0.5 21,863 + GFF1984 0.39 -0.9 21,863 + GFF1984 0.39 +1.1 21,863 + GFF1984 0.39 -0.9 21,863 + GFF1984 0.39 -1.1 21,864 - GFF1984 0.39 -0.5 21,864 - GFF1984 0.39 -0.2 21,864 - GFF1984 0.39 +0.6 21,864 - GFF1984 0.39 -1.9 21,864 - GFF1984 0.39 -0.2 21,872 + GFF1984 0.45 -0.1 21,872 + GFF1984 0.45 -1.4 21,872 + GFF1984 0.45 -0.3 21,872 + GFF1984 0.45 -0.9 21,872 + GFF1984 0.45 +0.3 21,872 + GFF1984 0.45 -0.4 21,872 + GFF1984 0.45 -1.8 21,872 + GFF1984 0.45 -0.3 21,872 + GFF1984 0.45 +0.7 21,873 - GFF1984 0.46 +0.1 21,873 - GFF1984 0.46 +1.7 21,873 - GFF1984 0.46 -0.4 21,873 - GFF1984 0.46 -1.9 21,873 - GFF1984 0.46 +0.1 21,939 + -0.9 21,939 + -0.5 21,940 - +0.2 21,946 - -3.5 21,946 - -2.2 21,946 - -0.6 21,953 - -0.9 22,006 - -1.0 22,027 + +0.3 22,028 - -0.6 22,029 + +1.1 22,029 + +0.1 22,029 + -1.3 22,029 + -0.1 22,029 + +0.8 22,030 - -1.3 22,030 - +0.2 22,030 - -1.2 22,030 - -1.9 22,030 - -0.5 22,030 - -0.9 22,030 - -0.8 22,030 - -1.3 22,030 - -0.2 22,030 - -1.1 22,030 - -0.8 22,030 - +0.1 22,251 - GFF1985 0.26 +1.4 22,265 + GFF1985 0.27 -1.5 22,265 + GFF1985 0.27 -1.5 22,265 + GFF1985 0.27 -0.8 22,266 - GFF1985 0.27 -2.9 22,287 + GFF1985 0.29 +1.1 22,300 + GFF1985 0.30 +0.1 22,300 + GFF1985 0.30 -0.7 22,300 + GFF1985 0.30 -1.3 22,301 - GFF1985 0.30 -2.3 22,301 - GFF1985 0.30 -0.5 22,301 - GFF1985 0.30 +0.1 22,301 - GFF1985 0.30 +0.3 22,301 - GFF1985 0.30 +0.3 22,302 + GFF1985 0.30 -0.9 22,303 - GFF1985 0.30 +0.2 22,315 - GFF1985 0.31 -0.5 22,321 + GFF1985 0.31 -0.4 22,321 + GFF1985 0.31 -1.0 22,321 + GFF1985 0.31 +0.1 22,321 + GFF1985 0.31 -0.4 22,322 - GFF1985 0.31 +0.1 22,322 - GFF1985 0.31 -1.1 22,322 - GFF1985 0.31 +0.7 22,365 + GFF1985 0.35 -0.2 22,407 + GFF1985 0.38 +0.1 22,408 - GFF1985 0.38 -1.3 22,408 - GFF1985 0.38 -0.4 22,411 + GFF1985 0.39 -1.4 22,411 + GFF1985 0.39 -0.0 22,411 + GFF1985 0.39 -1.5 22,411 + GFF1985 0.39 +0.3 22,411 + GFF1985 0.39 -2.3 22,412 - GFF1985 0.39 +0.5 22,418 - GFF1985 0.39 -0.5 22,418 - GFF1985 0.39 -1.3 22,487 + GFF1985 0.45 -2.1 22,488 - GFF1985 0.45 -1.1 22,488 - GFF1985 0.45 -1.5 22,488 - GFF1985 0.45 -1.1 22,488 - GFF1985 0.45 +0.2 22,488 - GFF1985 0.45 -1.3 22,488 - GFF1985 0.45 -0.8 22,555 + GFF1985 0.51 -0.0 22,638 - GFF1985 0.57 -1.1 22,638 - GFF1985 0.57 +0.6 22,707 + GFF1985 0.63 -0.3 22,707 + GFF1985 0.63 -0.3 22,708 - GFF1985 0.63 -1.3 22,718 + GFF1985 0.64 -0.9 22,719 - GFF1985 0.64 +0.1 22,800 + GFF1985 0.71 -0.4 22,801 - GFF1985 0.71 -2.0 22,801 - GFF1985 0.71 +0.6 22,827 + GFF1985 0.73 -0.4 22,829 + GFF1985 0.73 -0.5 22,829 + GFF1985 0.73 -0.3 22,829 + GFF1985 0.73 -1.3 22,829 + GFF1985 0.73 -0.3 22,829 + GFF1985 0.73 -0.4 22,830 - GFF1985 0.73 -1.3 22,830 - GFF1985 0.73 +0.1 23,010 + GFF1985 0.88 +0.2 23,010 + GFF1985 0.88 +0.8 23,011 - GFF1985 0.88 -0.3 23,223 + +0.1 23,224 - -1.1 23,224 - +0.1 23,230 - +0.2 23,275 + +0.1 23,326 - GFF1986 0.14 +0.6 23,359 - GFF1986 0.17 -1.1 23,723 - GFF1986 0.49 -0.4 23,723 - GFF1986 0.49 -0.0 23,723 - GFF1986 0.49 -1.4 23,736 + GFF1986 0.50 -0.3 23,736 + GFF1986 0.50 +0.1 23,737 - GFF1986 0.50 -0.8 23,737 - GFF1986 0.50 -0.9 23,737 - GFF1986 0.50 +0.1 23,737 - GFF1986 0.50 +0.1 23,737 - GFF1986 0.50 +0.3 23,737 - GFF1986 0.50 -0.3 23,842 + GFF1986 0.60 -1.3 23,842 + GFF1986 0.60 -0.9 23,842 + GFF1986 0.60 -0.8 23,846 + GFF1986 0.60 +0.8 23,847 - GFF1986 0.60 -0.8 23,882 - GFF1986 0.63 -1.3 23,895 + GFF1986 0.64 -0.5 23,991 + GFF1986 0.73 +0.5 23,992 - GFF1986 0.73 -1.0 23,992 - GFF1986 0.73 -0.2 24,061 - GFF1986 0.79 -0.2
Or see this region's nucleotide sequence